##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937344_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1697618 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04876597679808 31.0 31.0 34.0 30.0 34.0 2 32.26737758435643 33.0 31.0 34.0 30.0 34.0 3 32.3274888696986 34.0 31.0 34.0 30.0 34.0 4 36.00433254124308 37.0 35.0 37.0 35.0 37.0 5 35.91532959711785 37.0 35.0 37.0 35.0 37.0 6 35.95524493731806 37.0 35.0 37.0 35.0 37.0 7 35.889596481658415 37.0 35.0 37.0 35.0 37.0 8 35.85856653263573 37.0 35.0 37.0 35.0 37.0 9 37.540706448682805 39.0 37.0 39.0 35.0 39.0 10-11 37.44623171997469 39.0 37.0 39.0 34.5 39.0 12-13 37.343928080404424 39.0 37.0 39.0 34.0 39.0 14-15 38.48954888555612 40.0 38.0 41.0 34.0 41.0 16-17 38.45517925705312 40.0 38.0 41.0 34.0 41.0 18-19 38.44590538036237 40.0 38.0 41.0 34.0 41.0 20-21 38.34033775560815 40.0 38.0 41.0 34.0 41.0 22-23 38.18018717991916 40.0 37.5 41.0 33.5 41.0 24-25 38.11894018560123 40.0 37.5 41.0 33.0 41.0 26-27 38.0725227937027 40.0 37.0 41.0 33.0 41.0 28-29 37.941300398558454 40.0 37.0 41.0 33.0 41.0 30-31 38.04193846907843 40.0 37.0 41.0 33.0 41.0 32-33 37.99987305742516 40.0 37.0 41.0 33.0 41.0 34-35 37.87747155131484 40.0 37.0 41.0 33.0 41.0 36-37 37.71025018584864 40.0 37.0 41.0 33.0 41.0 38-39 37.59295230140114 40.0 37.0 41.0 33.0 41.0 40-41 37.42926412184602 40.0 37.0 41.0 32.5 41.0 42-43 37.22093692456136 40.0 36.0 41.0 32.0 41.0 44-45 36.93243120654941 39.0 36.0 41.0 31.0 41.0 46-47 36.59885675104765 39.0 36.0 41.0 30.5 41.0 48-49 36.28562256055249 39.0 35.5 40.5 30.0 41.0 50-51 36.04935916089485 39.0 35.0 40.0 29.5 41.0 52-53 35.80815118595585 39.0 35.0 40.0 28.5 41.0 54-55 35.581716263611725 38.5 35.0 40.0 28.0 41.0 56-57 35.30789582815451 38.0 34.5 40.0 28.0 41.0 58-59 35.030297157546634 38.0 34.0 40.0 27.0 41.0 60-61 34.692097397647764 38.0 34.0 40.0 26.0 41.0 62-63 34.324358895817554 37.0 33.5 40.0 26.0 41.0 64-65 33.921629895535986 37.0 33.0 39.5 25.5 41.0 66-67 33.48894833820094 36.0 32.5 39.0 24.5 40.0 68-69 33.04158532720553 36.0 32.0 39.0 23.0 40.0 70-71 32.606196152491314 35.0 31.5 38.0 22.0 40.0 72-73 32.11474577908575 35.0 31.0 37.5 21.5 39.5 74-75 31.620534478310198 35.0 31.0 37.0 20.0 39.0 76-77 30.382523924699196 33.5 29.5 35.5 20.0 38.0 78-79 31.016544947096463 34.0 30.5 36.0 20.0 38.5 80-81 30.81303008097228 34.0 31.0 36.0 18.5 37.5 82-83 30.435160030112783 34.0 30.0 35.0 18.0 37.0 84-85 30.037955240813893 34.0 30.0 35.0 14.0 36.5 86-87 29.600052249681614 34.0 29.5 35.0 7.5 36.0 88-89 29.089638835120738 34.0 29.0 35.0 4.5 36.0 90-91 28.52493729449146 33.0 29.0 35.0 2.0 35.0 92-93 28.094485626330542 33.0 27.5 35.0 2.0 35.0 94-95 27.582233753412133 33.0 27.0 35.0 2.0 35.0 96-97 27.021560504188813 33.0 26.0 34.0 2.0 35.0 98-99 26.15875656360854 32.0 25.0 34.0 2.0 35.0 100-101 23.595930297628797 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13738028790924162 1101 2 -0.10633751485200094 1101 3 -0.0810233504574569 1101 4 -0.1123644800783623 1101 5 -0.14569491149109837 1101 6 -0.1898625347111107 1101 7 -0.1806974478381349 1101 8 -0.11599672924587168 1101 9 -0.15548460504342643 1101 10-11 -0.12604136346710249 1101 12-13 -0.15435133993478445 1101 14-15 -0.3814261267059038 1101 16-17 -0.21067929597003143 1101 18-19 -0.09156232795404406 1101 20-21 -0.08843398927248813 1101 22-23 -0.039800319727028466 1101 24-25 -0.12379771893230895 1101 26-27 -0.32894549051814437 1101 28-29 -0.40256769228980005 1101 30-31 -0.23747839212667543 1101 32-33 -0.061558282772153916 1101 34-35 -0.10866522159174963 1101 36-37 -0.06605581683304962 1101 38-39 0.016416585877344403 1101 40-41 -0.060438051311002994 1101 42-43 -0.06904751444405122 1101 44-45 0.013078645232056374 1101 46-47 -0.11612000625752472 1101 48-49 -0.10257556272230062 1101 50-51 -0.14982187196163466 1101 52-53 -0.07395083534918712 1101 54-55 -0.1757476419798465 1101 56-57 -0.08559000883514045 1101 58-59 -0.12084728500209252 1101 60-61 0.05143686712085582 1101 62-63 -0.11623067593085779 1101 64-65 -0.168224303754549 1101 66-67 -0.1364380269053882 1101 68-69 -0.18288592249302127 1101 70-71 -0.1656933300122745 1101 72-73 -0.1466795037259203 1101 74-75 -0.3046870919455138 1101 76-77 -0.19425717779312635 1101 78-79 -0.33162126769429534 1101 80-81 -0.24920378373071728 1101 82-83 -0.051186446527516694 1101 84-85 -0.10392474236428129 1101 86-87 -0.15228799677968752 1101 88-89 -0.12004636860022799 1101 90-91 -0.04879951694410778 1101 92-93 -0.16310815952625646 1101 94-95 -0.09681607697233474 1101 96-97 -0.18989779786150152 1101 98-99 0.034022371200297385 1101 100-101 0.8111742482682338 1102 1 -0.12254392829733973 1102 2 -0.06511869167599826 1102 3 -0.10830512421789251 1102 4 -0.007072911026753559 1102 5 -0.0313788079096895 1102 6 -0.06656368120926714 1102 7 -0.16210160269641705 1102 8 -0.16932574860062033 1102 9 -0.312327465361804 1102 10-11 -0.17193526330939335 1102 12-13 -0.15382489150047007 1102 14-15 -0.149048738654308 1102 16-17 -0.2486097456759424 1102 18-19 -0.18508042454839568 1102 20-21 -0.0582830997779098 1102 22-23 -0.15505422428232407 1102 24-25 -0.22294403301216192 1102 26-27 -0.2607954513968238 1102 28-29 -0.24636345890657907 1102 30-31 -0.2444067320862615 1102 32-33 -0.25899780849048426 1102 34-35 -0.2869219413380506 1102 36-37 -0.3734654703200633 1102 38-39 -0.25251725346385 1102 40-41 -0.1787070354048126 1102 42-43 -0.187501611886141 1102 44-45 -0.1809834794475691 1102 46-47 -0.3534302368426907 1102 48-49 -0.1183386183893802 1102 50-51 -0.26143392783781394 1102 52-53 -0.16676339316769884 1102 54-55 -0.12224687282306235 1102 56-57 -0.13271702520134454 1102 58-59 -0.1215319314732426 1102 60-61 -0.08554955156355959 1102 62-63 -0.16531827510578267 1102 64-65 -0.032150182374785174 1102 66-67 -0.2609043085137088 1102 68-69 -0.31623924468002684 1102 70-71 -0.179180818439157 1102 72-73 -0.17691895024171345 1102 74-75 -0.19864979936986416 1102 76-77 -0.18672879578542734 1102 78-79 -0.21205385509706431 1102 80-81 -0.340770237916999 1102 82-83 -0.2008811271397022 1102 84-85 -0.28829754314335787 1102 86-87 -0.2000055501364706 1102 88-89 -0.13030618417199236 1102 90-91 0.018474739351017178 1102 92-93 -0.1521427110691711 1102 94-95 -0.04358662544803593 1102 96-97 0.08779499905531907 1102 98-99 0.14405762272953027 1102 100-101 0.6829973239505129 1103 1 -0.046380655698769147 1103 2 0.01986079012788622 1103 3 -0.0048379693512146105 1103 4 -0.050153772734454094 1103 5 -0.01301834879360797 1103 6 -0.1596089041653741 1103 7 -0.13961300389301812 1103 8 -0.10496114551378355 1103 9 -0.16277719964485016 1103 10-11 -0.21646862559097002 1103 12-13 -0.1699532641306618 1103 14-15 -0.2109942036373056 1103 16-17 -0.2575897310585873 1103 18-19 -0.20242430288134727 1103 20-21 -0.24170657597155554 1103 22-23 -0.12062947067217777 1103 24-25 -0.13375640808335731 1103 26-27 -0.16115432665215224 1103 28-29 -0.20473914977254282 1103 30-31 -0.35642794119545584 1103 32-33 -0.21847488943738114 1103 34-35 -0.22113461516412514 1103 36-37 -0.27609789438464816 1103 38-39 -0.24859180799830227 1103 40-41 -0.14312199636803058 1103 42-43 -0.12168611017883535 1103 44-45 -0.19722276379278725 1103 46-47 -0.07615990136349637 1103 48-49 -0.11118825225821638 1103 50-51 0.05939173025406319 1103 52-53 0.08928822139402115 1103 54-55 -0.03191432237377967 1103 56-57 0.007887932712513646 1103 58-59 -0.11364777645089674 1103 60-61 -0.14668734933371752 1103 62-63 -0.06808554595388472 1103 64-65 -0.16753550493846348 1103 66-67 0.14838573966174096 1103 68-69 0.11000691487226533 1103 70-71 0.06888845077985195 1103 72-73 0.262508364609225 1103 74-75 0.342114543484346 1103 76-77 0.4170593151571964 1103 78-79 0.2711828333297852 1103 80-81 0.1605711624767423 1103 82-83 0.13061203289619527 1103 84-85 0.06899545338021795 1103 86-87 -0.0421470829080981 1103 88-89 -0.07435127105629391 1103 90-91 -0.0948521560142872 1103 92-93 -0.04658378198821467 1103 94-95 0.004552733943992138 1103 96-97 0.1373169384668067 1103 98-99 0.46044634105749793 1103 100-101 1.1676590912782778 1104 1 -0.020751225426984377 1104 2 -0.0017718242536801654 1104 3 0.006033491169617378 1104 4 -0.05136942035964154 1104 5 -0.11150362228589472 1104 6 -0.10438506882933751 1104 7 -0.09080514975556753 1104 8 -0.024829611455182032 1104 9 -0.004732773732264661 1104 10-11 -0.0323717376792132 1104 12-13 -0.06596402945418589 1104 14-15 -0.10250040127954918 1104 16-17 -0.14541297505994777 1104 18-19 -0.09446781904829038 1104 20-21 -0.1201264614952322 1104 22-23 -0.21896463613217065 1104 24-25 -0.08908240027470526 1104 26-27 -0.36303806873925737 1104 28-29 -0.30053233426136927 1104 30-31 -0.26405653753372604 1104 32-33 -0.18309450203735622 1104 34-35 -0.153445748176523 1104 36-37 -0.26960515339681734 1104 38-39 -0.2881793899701748 1104 40-41 -0.3705784991541279 1104 42-43 -0.30632884548076333 1104 44-45 -0.2236902972448931 1104 46-47 -0.12380195435618191 1104 48-49 -0.10746360194170279 1104 50-51 -0.1256636126063242 1104 52-53 -0.08219883368349912 1104 54-55 -0.1539883529260706 1104 56-57 0.03161068772558906 1104 58-59 0.09479807472988 1104 60-61 0.10143554214120343 1104 62-63 0.22314109443124153 1104 64-65 0.09304521603495886 1104 66-67 0.13274209461416575 1104 68-69 0.09063748232612312 1104 70-71 0.131423798188969 1104 72-73 0.28215659629110945 1104 74-75 0.32498118993444436 1104 76-77 0.22362976358598985 1104 78-79 0.06388629415386049 1104 80-81 0.09926416122583959 1104 82-83 0.09835889616900673 1104 84-85 0.11991050588012087 1104 86-87 0.030467109124042935 1104 88-89 0.07046469497986863 1104 90-91 0.033654723937193154 1104 92-93 0.20349752652927933 1104 94-95 0.2772969437919848 1104 96-97 0.2854801999049634 1104 98-99 0.04218907256881366 1104 100-101 0.4373584961529957 1105 1 0.010806045146864562 1105 2 -0.012096574005340699 1105 3 -0.028130182705218942 1105 4 0.006255703809380009 1105 5 -0.11163505042068778 1105 6 -0.13150893220520743 1105 7 -0.07756300105838676 1105 8 -0.012214038749441158 1105 9 0.050093389635961216 1105 10-11 -0.06390646679971468 1105 12-13 -0.03969215660327308 1105 14-15 0.008862019443306224 1105 16-17 -0.09810801594021967 1105 18-19 -0.06134334437876987 1105 20-21 -0.12764804780225347 1105 22-23 -0.09250440842528462 1105 24-25 -0.1584711066245177 1105 26-27 -0.08338727023693338 1105 28-29 -0.025708845302865768 1105 30-31 -0.16612156389576427 1105 32-33 -0.07990928006135078 1105 34-35 -0.14844269170826863 1105 36-37 -0.04204688947078239 1105 38-39 -0.19096688901503 1105 40-41 -0.12029692201651443 1105 42-43 -0.06631050667125038 1105 44-45 -0.20112171773851628 1105 46-47 -0.14042608517302568 1105 48-49 -0.023402701811797044 1105 50-51 -0.2244914570320944 1105 52-53 -0.35963063623564295 1105 54-55 -0.05396581147271462 1105 56-57 -0.15871444408460178 1105 58-59 -0.06609785252617684 1105 60-61 0.033871547337199104 1105 62-63 -0.044563497912299965 1105 64-65 0.031934189825747694 1105 66-67 -0.025849255580681074 1105 68-69 -0.053811589271653304 1105 70-71 -0.07324419294727136 1105 72-73 -0.11076615147383961 1105 74-75 0.18975080864003147 1105 76-77 0.14142834232825408 1105 78-79 -0.0731290645990974 1105 80-81 -0.02944715534785658 1105 82-83 -0.12332177931047639 1105 84-85 -0.03109851070122005 1105 86-87 -0.010968666836834728 1105 88-89 0.050986968992248194 1105 90-91 0.26863753130328405 1105 92-93 0.31323123173291734 1105 94-95 0.5058993736842474 1105 96-97 0.4981137293616733 1105 98-99 0.7238361268975346 1105 100-101 1.1149675738637015 1106 1 -0.2075780038058035 1106 2 -0.0659061228667639 1106 3 -0.07832409617329006 1106 4 -0.06806970913012833 1106 5 -0.1157221117315359 1106 6 -0.1822400328347058 1106 7 -0.14228357331273145 1106 8 -0.21200779504813738 1106 9 -0.2371722742813347 1106 10-11 -0.26698027064379204 1106 12-13 -0.28901604881401255 1106 14-15 -0.33046538277383064 1106 16-17 -0.2013081635742111 1106 18-19 -0.17426608642535513 1106 20-21 -0.22673503513170346 1106 22-23 -0.26261127866683864 1106 24-25 -0.2782866545554228 1106 26-27 -0.261178752840614 1106 28-29 -0.33386336285371954 1106 30-31 -0.29904250572830193 1106 32-33 -0.2684034051162243 1106 34-35 -0.2112161146303393 1106 36-37 -0.22524004175244983 1106 38-39 -0.29614213450997084 1106 40-41 -0.19742561348284227 1106 42-43 -0.38214655402592257 1106 44-45 -0.1239026045617706 1106 46-47 -0.24898741821920112 1106 48-49 -0.22565695615327996 1106 50-51 0.0120622181172223 1106 52-53 0.01137703974555393 1106 54-55 0.032644623850274 1106 56-57 -0.20404880712941775 1106 58-59 -0.1436112472695541 1106 60-61 0.023916528028060213 1106 62-63 0.07417212709078314 1106 64-65 0.10948642480382631 1106 66-67 0.061149993097110666 1106 68-69 -0.062447684632026323 1106 70-71 -0.06493464326383247 1106 72-73 -0.12007547756905268 1106 74-75 -0.15574582027420192 1106 76-77 -0.013208606284269564 1106 78-79 0.02788678221665819 1106 80-81 -0.02714695033717618 1106 82-83 -0.05309349737229496 1106 84-85 -0.1439232273935609 1106 86-87 0.1754260305052604 1106 88-89 0.31573536647293565 1106 90-91 0.2551425020313083 1106 92-93 0.4726574484392323 1106 94-95 0.37330682706363305 1106 96-97 0.4800844623200611 1106 98-99 0.5697937010310063 1106 100-101 1.1763006200396013 1107 1 -0.04947580877729507 1107 2 -0.05239771561915063 1107 3 -0.007079258922068732 1107 4 0.0021235098388032725 1107 5 0.04091640949764752 1107 6 -0.01858823903430107 1107 7 -0.021781882570046207 1107 8 -0.028922761693557675 1107 9 -0.024722586698324278 1107 10-11 -0.006975869540184476 1107 12-13 -0.015629487259495534 1107 14-15 0.05417923687701176 1107 16-17 0.0733969756348003 1107 18-19 0.10958865786789573 1107 20-21 0.06644071180658528 1107 22-23 0.0775383588558114 1107 24-25 0.10671259585421922 1107 26-27 0.09636028503605587 1107 28-29 0.036427039847261256 1107 30-31 0.025821625540309867 1107 32-33 0.08900850924987225 1107 34-35 -0.06575372258669177 1107 36-37 -0.0329964169965038 1107 38-39 0.008455221496355136 1107 40-41 -0.07689323775483814 1107 42-43 -0.20536881940195428 1107 44-45 0.01904186525866436 1107 46-47 0.10864761982579552 1107 48-49 0.23779087016620082 1107 50-51 0.2735721561072495 1107 52-53 0.14756971690531628 1107 54-55 0.22981976410257943 1107 56-57 0.28038294639475936 1107 58-59 0.15017030895015893 1107 60-61 0.3119799372351366 1107 62-63 0.19531622991879516 1107 64-65 0.1868299028855418 1107 66-67 0.3011396741749053 1107 68-69 0.40233362347766644 1107 70-71 0.5253077067501479 1107 72-73 0.6846772759700173 1107 74-75 0.6471031491048116 1107 76-77 0.5991957551918716 1107 78-79 0.5557912695012384 1107 80-81 0.5689573277634743 1107 82-83 0.44318266048178856 1107 84-85 0.4589720763132732 1107 86-87 0.4237992879268653 1107 88-89 0.5960427072858074 1107 90-91 0.4874243339775326 1107 92-93 0.7273211278495211 1107 94-95 0.8201674462165016 1107 96-97 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0.24174032372241072 2316 26-27 0.34878873885141815 2316 28-29 0.4331957464496483 2316 30-31 0.4259987110850574 2316 32-33 0.41076569493597503 2316 34-35 0.28197342069208275 2316 36-37 0.5304657089620619 2316 38-39 0.6176049002051727 2316 40-41 0.3618116041367685 2316 42-43 0.553478985410429 2316 44-45 0.3438139064543577 2316 46-47 0.2227474090836452 2316 48-49 -0.019961800356306014 2316 50-51 0.012394278337708897 2316 52-53 0.6081086368906128 2316 54-55 0.6863923821207649 2316 56-57 0.5905609639686844 2316 58-59 0.4153649736008873 2316 60-61 0.2985794287614354 2316 62-63 0.8270805611619707 2316 64-65 0.3957740173801412 2316 66-67 0.2359092817388344 2316 68-69 0.5630504056986823 2316 70-71 0.8695009060438821 2316 72-73 0.6396343767379093 2316 74-75 0.8489146513805146 2316 76-77 0.7483463141504849 2316 78-79 0.5427765175504504 2316 80-81 0.5914140531307197 2316 82-83 0.7070569504567779 2316 84-85 0.5532830737398378 2316 86-87 0.31953629000321726 2316 88-89 0.4278463908722543 2316 90-91 0.41980173330543735 2316 92-93 0.5066839305648294 2316 94-95 0.5974759012745707 2316 96-97 0.7099373221725536 2316 98-99 0.18788568558827023 2316 100-101 -0.4273112131912278 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 13.0 5 43.0 6 76.0 7 190.0 8 435.0 9 837.0 10 1338.0 11 2193.0 12 3389.0 13 4779.0 14 6066.0 15 8576.0 16 11438.0 17 16099.0 18 14379.0 19 9367.0 20 8575.0 21 8866.0 22 9519.0 23 10777.0 24 12400.0 25 14785.0 26 18159.0 27 22827.0 28 28013.0 29 35536.0 30 44013.0 31 55486.0 32 69839.0 33 91091.0 34 122616.0 35 173012.0 36 255251.0 37 361614.0 38 257431.0 39 18580.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.04345245358 20.544691165037154 15.435824254459568 21.976032126923272 2 15.631726336549212 21.739343008851225 39.34012245393251 23.288808200667052 3 19.432875947356827 25.157956619215867 30.54191225587853 24.867255177548778 4 12.854717610204416 17.66752001922694 37.90063488959236 31.577127480976287 5 13.18674392192161 38.43615838112697 34.45141778630235 13.925679910649066 6 31.247783659221334 37.72792230054111 17.187965726093857 13.836328314143701 7 28.011484326862696 33.409165077184625 20.801087170376377 17.778263425576306 8 26.29272309789364 35.48371895208462 19.641639049538824 18.581918900482915 9 26.060927723433657 16.996992256208404 20.63603237006205 36.30604765029589 10-11 24.553240870279776 27.499344668844234 27.482998206307087 20.46441625456891 12-13 25.648668025121395 24.69872172119631 28.154003759608887 21.498606494073403 14-15 22.301807591578317 26.157239143317284 26.840254992583727 24.700698272520675 16-17 21.52736952600644 29.59596917563315 27.23731133859325 21.639349959767156 18-19 21.36637924432941 28.208819652006518 29.65313751385765 20.771663589806423 20-21 22.37175854638676 26.908674389644784 29.86693708478586 20.852629979182595 22-23 21.920339584096663 26.976813229924968 29.78647577824301 21.316371407735364 24-25 21.90619838313293 27.143786183350183 29.815699067627076 21.13431636588981 26-27 21.743431874725168 27.269725729355628 29.93125930167179 21.055583094247417 28-29 21.430786799017763 27.735705842901748 29.813634959210468 21.01987239887002 30-31 21.72234342191956 27.206195292621427 29.769060824576176 21.302400460882833 32-33 21.508460678432957 27.29860310152225 29.80010815153939 21.392828068505402 34-35 22.068745736673385 27.14035195197035 29.69569714741479 21.095205163941475 36-37 21.99011261370715 27.018041461047616 29.71676481893006 21.275081106315174 38-39 21.634611278159834 27.48375140675839 29.79579802999337 21.085839285088408 40-41 21.90963035526575 27.41926695458014 29.495707658355112 21.175395031798995 42-43 21.752038927783353 27.47894647076307 29.212038768716496 21.556975832737077 44-45 21.963254330339918 27.330519581883994 29.160528152731917 21.545697935044164 46-47 22.079172110569047 27.756803945292756 28.81720151412155 21.346822430016648 48-49 22.170859404176912 27.81786008395293 28.575981168908438 21.435299342961724 50-51 21.94324636048864 27.987273933240576 28.61309199124891 21.456387715021872 52-53 22.355205941501563 28.14844093311923 28.079992083024568 21.41636104235464 54-55 22.063149660288712 27.897029838279288 28.25906652733418 21.780753974097824 56-57 22.214449893910174 28.10122772025273 28.187760732979978 21.49656165285712 58-59 22.569300042765803 27.82227803899346 28.163815416660288 21.44460650158045 60-61 22.205525624728295 27.84133415173496 28.712613791795327 21.240526431741415 62-63 21.97317064262985 27.949338425959198 28.680215454831416 21.397275476579537 64-65 21.90740201859311 28.04087845439905 28.424356951917336 21.62736257509051 66-67 22.012696613725822 27.939884002172455 28.204607868201208 21.842811515900515 68-69 21.90301351658618 27.986802684702923 28.27927130838622 21.83091249032468 70-71 22.135309592617418 27.845604841607475 28.295411570800972 21.723673994974135 72-73 22.1036475814936 27.667708518642005 28.431661304251016 21.796982595613382 74-75 21.858568888878416 28.317324627802016 27.91408314473574 21.910023338583827 76-77 21.95876810919771 27.850935840689715 28.08632448524933 22.103971564863237 78-79 22.04583127652982 27.900947091748556 28.072363747321244 21.98085788440038 80-81 22.07644598281002 27.989293226917493 28.03317827283775 21.901082517434734 82-83 21.991596997539183 27.866600495108557 28.21738829499752 21.92441421235474 84-85 21.931993762992537 27.904128689232916 28.10423366040119 22.05964388737336 86-87 21.905281400173653 27.882862929115976 28.069330084860077 22.142525585850294 88-89 21.845962990496094 27.884099956527326 27.834353782770915 22.435583270205665 90-91 21.953688040382534 27.77456874701209 27.96340899805681 22.308334214548566 92-93 22.027505811989325 27.74689209890499 27.985144634811498 22.240457454294184 94-95 21.77450993097387 27.697750612917627 27.99269329142363 22.53504616468487 96-97 21.605626236291087 27.626974973168288 28.130268411385835 22.637130379154794 98-99 21.714870063332953 27.716700277536344 27.739114069116354 22.829315590014346 100-101 21.608757958734614 27.520321191921305 28.072686462308404 22.798234387035677 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 230.0 1 170.0 2 153.0 3 214.0 4 209.0 5 207.5 6 398.5 7 908.5 8 1510.0 9 1869.5 10 1825.5 11 1703.5 12 1814.0 13 1966.0 14 2160.0 15 2312.0 16 2443.5 17 2592.5 18 2722.0 19 2937.0 20 3219.5 21 3665.5 22 4284.0 23 5088.0 24 6278.0 25 7799.0 26 9871.0 27 12663.5 28 15107.0 29 17848.5 30 21702.0 31 24974.5 32 28588.0 33 32813.0 34 37168.5 35 41168.5 36 45285.0 37 49457.0 38 52181.0 39 53724.5 40 55062.5 41 57297.0 42 59195.5 43 61559.0 44 64269.5 45 65856.5 46 66361.0 47 69937.0 48 77059.0 49 74920.0 50 66199.5 51 60197.0 52 55253.0 53 52196.0 54 49262.0 55 44432.0 56 38659.0 57 32980.0 58 29041.0 59 26099.5 60 22102.5 61 17536.5 62 13518.5 63 10166.0 64 7553.5 65 5709.0 66 4045.5 67 3047.5 68 2226.0 69 1363.5 70 943.0 71 682.5 72 468.5 73 349.0 74 289.0 75 225.5 76 155.5 77 103.5 78 66.5 79 41.5 80 24.0 81 12.5 82 10.5 83 9.5 84 6.0 85 4.5 86 2.0 87 0.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06461995572619988 2 0.0 3 0.0 4 0.0 5 0.0024740548226986282 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.767182016213306E-4 12-13 0.005949512787918131 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.068728064853224E-4 24-25 8.246849408995429E-4 26-27 3.828894368462163E-4 28-29 0.0036816292004443873 30-31 3.828894368462163E-4 32-33 0.0 34-35 0.0 36-37 2.94530336035551E-5 38-39 5.596076384675469E-4 40-41 8.83591008106653E-5 42-43 0.012870975684753578 44-45 1.767182016213306E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.89060672071102E-5 82-83 6.185137056746571E-4 84-85 5.301546048639917E-4 86-87 0.0 88-89 0.0 90-91 0.008924269181877194 92-93 0.0029158503267519547 94-95 0.0 96-97 0.0 98-99 1.472651680177755E-4 100-101 0.011221605802954492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1697618.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.4882815218592 #Duplication Level Percentage of deduplicated Percentage of total 1 78.38352368109737 45.8451759973534 2 12.618512901242074 14.760702699101172 3 3.915246700640923 6.8698815376385 4 1.7001643707891039 3.977587694085908 5 0.9011143404144267 2.6352314612771726 6 0.52595252712095 1.8457235684030042 7 0.3427692959261379 1.4033590954024193 8 0.25716232529111815 1.203278598275428 9 0.18199252364112165 0.9579986961835985 >10 1.05649750789544 11.397610674881156 >50 0.0794852152253726 3.157097566949249 >100 0.03516746321195009 3.499176876856195 >500 0.001004644793324752 0.37112782841914654 >1k 0.0012055737519897026 1.3097004698597778 >5k 2.009289586649504E-4 0.7663472353138867 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7420 0.43708301867675764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5622 0.3311699098383735 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4964 0.292409717616095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4092 0.24104362701149493 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1732 0.10202530840271486 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1698 0.10002250211767312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.89060672071102E-5 0.0 0.0 0.0 0.0 3 5.89060672071102E-5 0.0 0.0 0.0 0.0 4 5.89060672071102E-5 0.0 0.0 0.0 0.0 5 1.767182016213306E-4 0.0 0.0 0.0 0.0 6 2.94530336035551E-4 0.0 0.0 0.0 0.0 7 4.123424704497714E-4 0.0 0.0 0.0 0.0 8 4.712485376568816E-4 0.0 0.0 0.0 0.0 9 4.712485376568816E-4 0.0 0.0 0.0 0.0 10-11 4.712485376568816E-4 0.0 0.0 0.0 0.0 12-13 6.479667392782121E-4 0.0 0.0 0.0 0.0 14-15 8.246849408995427E-4 0.0 0.0 0.0 0.0 16-17 0.0010014031425208735 0.0 0.0 0.0 0.0 18-19 0.001207574377745759 2.94530336035551E-5 0.0 0.0 0.0 20-21 0.0013548395457635345 5.89060672071102E-5 0.0 0.0 0.0 22-23 0.0013548395457635345 5.89060672071102E-5 0.0 0.0 0.0 24-25 0.0014137456129706448 5.89060672071102E-5 0.0 1.767182016213306E-4 0.0 26-27 0.00150210471378131 5.89060672071102E-5 0.0 3.828894368462163E-4 0.0 28-29 0.0017966350498168611 5.89060672071102E-5 0.0 0.0013548395457635347 0.0 30-31 0.0019439002178346365 5.89060672071102E-5 0.0 0.0035049109988230572 0.0 32-33 0.0019439002178346365 5.89060672071102E-5 0.0 0.006155684023143016 0.0 34-35 0.002061712352248857 5.89060672071102E-5 0.0 0.009719501089173181 0.0 36-37 0.0022973366210772977 5.89060672071102E-5 0.0 0.015521748709073538 0.0 38-39 0.002503507856302183 5.89060672071102E-5 0.0 0.025388514966264496 0.0 40-41 0.0025329608899057384 5.89060672071102E-5 0.0 0.042206197153894454 0.0 42-43 0.0028569442595448445 1.178121344142204E-4 0.0 0.05872934900548887 0.0 44-45 0.003534364032426612 1.178121344142204E-4 0.0 0.07946428466239167 0.0 46-47 0.0037994413348586076 1.178121344142204E-4 0.0 0.11368870970972268 0.0 48-49 0.003946706502876383 1.178121344142204E-4 0.0 0.14290611904444933 0.0 50-51 0.003946706502876383 1.178121344142204E-4 0.0 0.18290333867807718 0.0 52-53 0.0039761595364799385 1.178121344142204E-4 0.0 0.22770140278908446 0.0 54-55 0.004005612570083494 1.178121344142204E-4 0.0 0.2823956861908863 0.0 56-57 0.004300142906119044 1.178121344142204E-4 0.0 0.37682211192388393 0.0 58-59 0.004359048973326155 1.178121344142204E-4 0.0 0.4598502136523058 0.0 60-61 0.004417955040533265 1.178121344142204E-4 0.0 0.5421714425742423 0.0 62-63 0.004535767174947485 1.178121344142204E-4 0.0 0.6344477968541804 0.0 64-65 0.0045946732421545955 1.178121344142204E-4 0.0 0.7333510836949184 0.0 66-67 0.0045946732421545955 1.178121344142204E-4 0.0 0.8671856683894728 0.0 68-69 0.0045946732421545955 1.178121344142204E-4 0.0 1.0372180313827963 0.0 70-71 0.004800844477379482 1.767182016213306E-4 0.0 1.1873990497273237 0.0 72-73 0.005007015712604367 1.767182016213306E-4 0.0 1.357519771821458 0.0 74-75 0.005124827847018587 1.767182016213306E-4 0.0 1.5647218632224682 0.0 76-77 0.005124827847018587 1.767182016213306E-4 0.0 1.8001105077820805 0.0 78-79 0.005124827847018587 1.767182016213306E-4 0.0 2.071196229069202 0.0 80-81 0.005566623351071914 1.767182016213306E-4 0.0 2.36669851521367 0.0 82-83 0.005654982451882579 1.767182016213306E-4 0.0 2.6864111949802605 0.0 84-85 0.005772794586296799 1.767182016213306E-4 0.0 3.0268882634373577 0.0 86-87 0.00589060672071102 1.767182016213306E-4 0.0 3.3550834168817723 0.0 88-89 0.006096777955935905 1.767182016213306E-4 0.0 3.7721972787753195 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGCG 60 1.7121753E-4 23.749182 80-81 GTACATG 7215 0.0 23.71937 1 TACATGG 7170 0.0 22.921108 2 GAGTACT 6495 0.0 22.489666 12-13 TCGCGGA 150 7.2759576E-12 22.165903 82-83 GTACTTT 6750 0.0 21.602324 14-15 ACATGGG 7245 0.0 21.503746 3 ATTCGCG 145 1.05501385E-10 21.292372 80-81 GTATCAA 12980 0.0 21.13188 1 GGTATCA 10125 0.0 20.658302 1 CGCGGAA 150 1.6734703E-10 20.582624 82-83 AGTACTT 6735 0.0 20.207104 12-13 TATCGTT 120 0.00476212 19.793318 7 AATTCGC 180 3.6379788E-12 19.790985 78-79 AGAGTAC 10510 0.0 19.390295 10-11 AGGATCG 205 1.5901898E-5 18.536493 5 CATGGGG 3415 0.0 18.49863 4 TCAACGC 14780 0.0 18.382318 4 ATCAACG 14785 0.0 18.3761 3 CAACGCA 14805 0.0 18.351816 5 >>END_MODULE