FastQCFastQC Report
Thu 26 May 2016
SRR937343_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937343_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991065
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59460.5999606483933949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.4447740561920762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30930.3120885108443947No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17710.1786966546089308No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.10271778339463103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59500.035.392781
GGTATCA40300.034.679291
TATCAAC74650.028.0089552
ATCAACG75650.027.513083
TCAACGC76850.027.0230034
CAACGCA76950.026.931585
AACGCAG79100.026.0648636
GTACATG51950.025.4382461
TACATGG52200.025.0343482
GTACCTG10300.024.922091
TACCTGG9850.024.1216952
ACATGGG52200.023.5778033
CGCAGAG88200.022.9986068
ACGCAGA88650.022.9890377
GTGTAGA6000.022.9760131
GAGTACT46800.022.32816912-13
AGTACTT48350.022.20180112-13
TTAGCGC1302.9318492E-421.9332984
GCAGAGT90850.021.8571519
CATGGGA31700.021.2875214
Produced by FastQC (version 0.11.5)