FastQCFastQC Report
Thu 26 May 2016
SRR937343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937343_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991065
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67710.683204431596313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53120.5359890622713949No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37860.38201328873484586No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13480.13601529667579826No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11800.11906383536902222No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10000.10090155539747646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50600.033.622161
GTATCAA74600.031.5325831
TATCAAC91750.025.6255462
ATCAACG92300.025.3184683
TCAACGC92400.025.2910674
CAACGCA93550.025.0817095
AACGCAG96550.024.3515666
GAGTACT57650.023.11390512-13
TACCTGG8400.022.0525762
GTACTTT60000.021.92817714-15
AGTACTT58550.021.78497912-13
GTGTACG1100.002869427421.6008341
ACGCAGA109150.021.5437457
CGCAGAG109200.021.533888
GTACATG53450.021.4270551
TACATGG54600.021.1391562
TGCACCG1604.990132E-520.7803125
ACATGGG54650.020.7721653
GCAGAGT113750.020.5054749
AGAGTAC92900.020.04518510-11
Produced by FastQC (version 0.11.5)