##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937343_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 991065 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.282244857804486 33.0 31.0 34.0 31.0 34.0 2 32.486935771114915 34.0 31.0 34.0 31.0 34.0 3 32.54795699575709 34.0 31.0 34.0 31.0 34.0 4 36.16162713848234 37.0 35.0 37.0 35.0 37.0 5 36.099109543773615 37.0 35.0 37.0 35.0 37.0 6 36.14135904304965 37.0 35.0 37.0 35.0 37.0 7 36.087337359305394 37.0 35.0 37.0 35.0 37.0 8 36.059472385766824 37.0 35.0 37.0 35.0 37.0 9 37.76464813105094 39.0 37.0 39.0 35.0 39.0 10-11 37.68864857501778 39.0 37.0 39.0 35.0 39.0 12-13 37.58183620650512 39.0 37.0 39.0 35.0 39.0 14-15 38.799392068128725 40.0 38.0 41.0 35.0 41.0 16-17 38.76375767482456 40.0 38.0 41.0 35.0 41.0 18-19 38.77325755626523 40.0 38.0 41.0 35.0 41.0 20-21 38.683850201550854 40.0 38.0 41.0 34.0 41.0 22-23 38.622914743230766 40.0 38.0 41.0 34.0 41.0 24-25 38.65357014928385 40.0 38.0 41.0 34.0 41.0 26-27 38.49626765146584 40.0 38.0 41.0 34.0 41.0 28-29 38.352760414301784 40.0 38.0 41.0 34.0 41.0 30-31 38.36639120542043 40.0 38.0 41.0 34.0 41.0 32-33 38.24154117035714 40.0 38.0 41.0 34.0 41.0 34-35 38.10114170109932 40.0 37.0 41.0 34.0 41.0 36-37 37.930609495845374 40.0 37.0 41.0 33.0 41.0 38-39 37.80515657398859 40.0 37.0 41.0 33.0 41.0 40-41 37.608726975526324 40.0 37.0 41.0 33.0 41.0 42-43 37.38092556996766 40.0 37.0 41.0 33.0 41.0 44-45 37.04379934716694 40.0 36.0 41.0 31.5 41.0 46-47 36.625622436469854 39.5 36.0 41.0 31.0 41.0 48-49 36.23693198730659 39.0 36.0 41.0 30.0 41.0 50-51 35.96923814280597 39.0 35.0 41.0 29.0 41.0 52-53 35.71986549822665 39.0 35.0 40.0 28.5 41.0 54-55 35.50198271556356 39.0 35.0 40.0 28.0 41.0 56-57 35.19662887903417 38.0 35.0 40.0 27.5 41.0 58-59 34.93004142008849 38.0 34.0 40.0 27.0 41.0 60-61 34.57158006790675 38.0 34.0 40.0 26.0 41.0 62-63 34.18046697239838 37.0 34.0 40.0 26.0 41.0 64-65 33.76069430360269 37.0 33.0 39.5 25.0 41.0 66-67 33.32578387895849 36.0 33.0 39.0 23.5 40.0 68-69 32.86583927391241 35.5 32.0 39.0 22.0 40.0 70-71 32.42346011613769 35.0 32.0 38.0 21.5 40.0 72-73 31.937046006064186 35.0 31.0 37.5 20.0 39.5 74-75 31.461246235110714 35.0 31.0 37.0 18.5 39.0 76-77 30.17025523048438 33.5 29.5 35.5 18.0 38.0 78-79 30.799329004656606 34.0 31.0 36.0 18.0 38.0 80-81 30.621026370621504 34.0 31.0 35.5 16.0 37.0 82-83 30.23187530585784 34.0 30.0 35.0 10.0 37.0 84-85 29.804648030149387 34.0 30.0 35.0 7.0 36.5 86-87 29.443913365924537 34.0 29.5 35.0 2.0 36.0 88-89 28.988462411648072 34.0 29.0 35.0 2.0 36.0 90-91 28.437602982649977 34.0 29.0 35.0 2.0 35.0 92-93 27.935905818488195 33.0 27.5 35.0 2.0 35.0 94-95 27.45497419442721 33.0 27.0 35.0 2.0 35.0 96-97 26.79289098091447 33.0 25.5 35.0 2.0 35.0 98-99 25.848099771457974 32.0 24.5 34.0 2.0 35.0 100-101 22.94855130591838 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0878512570029315 1101 2 0.1380725075563518 1101 3 0.07970334468283369 1101 4 0.07523819060788384 1101 5 0.042523468412944965 1101 6 0.1434969818855123 1101 7 0.09637540760633811 1101 8 0.11468687546144452 1101 9 0.19172363085183264 1101 10-11 0.15999450973404095 1101 12-13 0.16423180473992716 1101 14-15 0.25500262216484515 1101 16-17 0.17580674719107492 1101 18-19 0.2907058919657146 1101 20-21 0.21325023332831705 1101 22-23 0.10454905886912513 1101 24-25 0.06921355318807798 1101 26-27 0.042576827862937705 1101 28-29 0.057816080330610475 1101 30-31 0.20079780254653912 1101 32-33 0.09872645829253202 1101 34-35 0.09155368761012284 1101 36-37 0.25716262508458243 1101 38-39 0.0735757762700402 1101 40-41 0.26095323868330667 1101 42-43 0.3094898324649691 1101 44-45 0.4125726947360775 1101 46-47 0.38212050973668 1101 48-49 0.5292417546643904 1101 50-51 0.452794020914709 1101 52-53 0.3598016862160165 1101 54-55 0.4723869259288449 1101 56-57 0.4174830390649049 1101 58-59 0.5757528073944513 1101 60-61 0.6940663849803954 1101 62-63 0.6514021694272714 1101 64-65 0.5291088301650291 1101 66-67 0.3456151591772212 1101 68-69 0.5056272938900079 1101 70-71 0.3950464471920583 1101 72-73 0.35211246989275224 1101 74-75 0.4269382683092289 1101 76-77 0.47158936972536836 1101 78-79 0.5197170139730467 1101 80-81 0.3941398845356261 1101 82-83 0.36523498265330545 1101 84-85 0.5008066987586375 1101 86-87 0.762178636563533 1101 88-89 0.950439821882707 1101 90-91 1.0572602340099344 1101 92-93 1.0473382479339044 1101 94-95 1.0984591224978146 1101 96-97 1.180018231365917 1101 98-99 1.446543384477824 1101 100-101 2.0209979625197256 1102 1 0.20227902513325802 1102 2 0.20189041874463953 1102 3 0.09250413853051498 1102 4 0.14965520871753313 1102 5 -0.0014802940942928444 1102 6 0.14814016389163243 1102 7 0.074582841659101 1102 8 0.08716785619409961 1102 9 0.11637632249162522 1102 10-11 0.13798487606167242 1102 12-13 0.12367786857851115 1102 14-15 0.20465583063255366 1102 16-17 0.222984866764385 1102 18-19 0.2899172125644114 1102 20-21 0.14085866382530554 1102 22-23 0.13655517811165652 1102 24-25 0.17917644494363572 1102 26-27 0.20201875707818573 1102 28-29 0.1262296253566575 1102 30-31 0.24421806633551313 1102 32-33 0.17771223518825963 1102 34-35 0.29429701019343923 1102 36-37 0.3945411705241 1102 38-39 0.3583458912125721 1102 40-41 0.3496098995615 1102 42-43 0.4153149999172072 1102 44-45 0.32479774228424674 1102 46-47 0.3912538926854907 1102 48-49 0.566803257186514 1102 50-51 0.6539315591599788 1102 52-53 0.6831938345663104 1102 54-55 0.7047088270286537 1102 56-57 0.7685079088242688 1102 58-59 0.6471195071381004 1102 60-61 0.61240395630265 1102 62-63 0.6167038935652087 1102 64-65 0.6837742357715726 1102 66-67 0.6771550996386395 1102 68-69 0.5929743128265841 1102 70-71 0.5367022604724667 1102 72-73 0.5775042254538221 1102 74-75 0.44853702378760474 1102 76-77 0.3599121597691983 1102 78-79 0.5105055900087656 1102 80-81 0.6303328267335928 1102 82-83 0.5933850488072814 1102 84-85 0.7028383493002721 1102 86-87 0.8835091583529966 1102 88-89 0.8868100360561968 1102 90-91 0.9310586091029585 1102 92-93 0.898473202039586 1102 94-95 0.7677543759502257 1102 96-97 0.6884730192597388 1102 98-99 1.2279220746291521 1102 100-101 1.8786470156909836 1103 1 0.04818757882165414 1103 2 0.07235534920482678 1103 3 0.04010582646279914 1103 4 0.06861385937048681 1103 5 0.010039448387836103 1103 6 0.012009007891066403 1103 7 -0.03866540840124344 1103 8 0.004172130125198237 1103 9 -0.044716963233362605 1103 10-11 0.07988469461727021 1103 12-13 0.11537579903037454 1103 14-15 0.09415707702239473 1103 16-17 0.016665732703607716 1103 18-19 0.029981987381930253 1103 20-21 0.08070916987206544 1103 22-23 -0.013471455928311116 1103 24-25 -0.08615035264598703 1103 26-27 -0.12052544918580566 1103 28-29 -0.08305462643490102 1103 30-31 -0.02963994718126628 1103 32-33 -0.009540671667508605 1103 34-35 -0.033163309248585904 1103 36-37 -0.14203303648897503 1103 38-39 -0.15799554885668243 1103 40-41 -0.2309695156548628 1103 42-43 -0.14571570621777852 1103 44-45 -0.1090983391826228 1103 46-47 -0.1576778326176722 1103 48-49 -0.08446950659452312 1103 50-51 -0.2102585059269586 1103 52-53 -0.3409430040563848 1103 54-55 -0.2931919216375576 1103 56-57 -0.28712940421726074 1103 58-59 -0.34788296899529314 1103 60-61 -0.2311447278093084 1103 62-63 -0.4026528631578117 1103 64-65 -0.38079276340443613 1103 66-67 -0.175395837962796 1103 68-69 -0.1023681634732796 1103 70-71 0.07891175176230547 1103 72-73 -0.17059133664926662 1103 74-75 -0.03869866457748117 1103 76-77 -0.1462266743257068 1103 78-79 -0.15048547718392413 1103 80-81 -0.0053985585694107385 1103 82-83 -0.06914735940932104 1103 84-85 -0.2912108674924916 1103 86-87 -0.31857037754885553 1103 88-89 -0.08824448049436029 1103 90-91 0.20536087008325765 1103 92-93 0.249177963706547 1103 94-95 0.3220540047382947 1103 96-97 0.48869977273673015 1103 98-99 0.5873427218363219 1103 100-101 1.4641089914373602 1104 1 0.14129948742957765 1104 2 0.15533869460671212 1104 3 0.171107144719727 1104 4 0.07094799638963423 1104 5 0.07707692454913229 1104 6 0.09126887426172203 1104 7 0.07820005777631422 1104 8 0.05888536282540002 1104 9 0.10201166416145213 1104 10-11 -0.009992577519234658 1104 12-13 -0.01552021781785129 1104 14-15 0.053196155250461175 1104 16-17 0.12990983834452408 1104 18-19 0.08061286748420571 1104 20-21 0.1034431339528723 1104 22-23 0.09252966339649049 1104 24-25 -0.07958081790845029 1104 26-27 -0.040419192902866996 1104 28-29 -0.13221738740070066 1104 30-31 0.04309627146371753 1104 32-33 0.12465867954849585 1104 34-35 0.12563148032101168 1104 36-37 0.10651303486665142 1104 38-39 0.055993358687622674 1104 40-41 0.03924928316639864 1104 42-43 0.010875976297150203 1104 44-45 0.22908124613672243 1104 46-47 0.018991672147414818 1104 48-49 0.021681209693774406 1104 50-51 0.0571580022571041 1104 52-53 0.041835839113481654 1104 54-55 0.1559758789422574 1104 56-57 0.023649534440032483 1104 58-59 -0.03910475710340222 1104 60-61 0.1363200545721952 1104 62-63 0.2816219496470538 1104 64-65 0.18850219459436346 1104 66-67 0.2477800206945986 1104 68-69 0.34457371916737145 1104 70-71 0.3041591570241877 1104 72-73 0.5101223257443337 1104 74-75 0.43824350983029703 1104 76-77 0.35285929883702494 1104 78-79 0.3779798542502739 1104 80-81 0.511350731242878 1104 82-83 0.2003550184927647 1104 84-85 0.23076954167111907 1104 86-87 0.252910446719806 1104 88-89 0.4860869086089288 1104 90-91 0.5674494452782817 1104 92-93 0.5938597120812688 1104 94-95 0.8241093715293566 1104 96-97 1.0262722171882501 1104 98-99 1.0542435979334321 1104 100-101 1.6456547206297216 1105 1 -0.021932698310159537 1105 2 -0.006594840136493474 1105 3 0.007938037895030448 1105 4 0.03489064990932178 1105 5 -0.004790100671364428 1105 6 -0.018352873577548223 1105 7 -0.003274660858522793 1105 8 -0.03175295152892943 1105 9 0.15936257105918372 1105 10-11 0.022935999442573518 1105 12-13 0.04103362612269734 1105 14-15 0.09165686372627846 1105 16-17 0.07446679196492312 1105 18-19 0.10960980207026694 1105 20-21 0.09042480786808227 1105 22-23 0.10210714533046428 1105 24-25 0.027479870647695748 1105 26-27 0.046259259561090005 1105 28-29 0.03353767827883303 1105 30-31 0.0950174117534317 1105 32-33 0.09888457952968821 1105 34-35 0.21790016329966733 1105 36-37 0.03426167092344201 1105 38-39 0.30643076116430024 1105 40-41 0.21561156640179036 1105 42-43 0.2440178595900946 1105 44-45 0.44507285774766814 1105 46-47 0.4723387279113851 1105 48-49 0.6573194133833411 1105 50-51 0.5710312136378732 1105 52-53 0.4834106540266703 1105 54-55 0.6015871910943602 1105 56-57 0.7413664097097765 1105 58-59 0.731114801786859 1105 60-61 0.6836776566881042 1105 62-63 0.8628070032641304 1105 64-65 0.8034969933504925 1105 66-67 1.0267943849808674 1105 68-69 0.9785331383986957 1105 70-71 0.8934592577291269 1105 72-73 0.7259072709099428 1105 74-75 0.775759694551919 1105 76-77 0.7174385514072164 1105 78-79 0.9061490164073511 1105 80-81 0.8709080168575625 1105 82-83 0.6780737750634884 1105 84-85 0.6903641766433992 1105 86-87 0.9264997476504924 1105 88-89 1.2098037506792458 1105 90-91 1.3262918365224827 1105 92-93 1.2295623779954177 1105 94-95 1.2553724438045144 1105 96-97 1.5819222066750953 1105 98-99 1.7555226059344946 1105 100-101 2.2189081540040014 1106 1 -0.1417813475930032 1106 2 0.030257788457845436 1106 3 -0.1161221308590612 1106 4 0.023204477772324594 1106 5 -0.14234766380749164 1106 6 -0.06821403764837441 1106 7 -0.11736699652860239 1106 8 -0.0773854860668095 1106 9 0.026696041273012838 1106 10-11 -0.24447104403653697 1106 12-13 -0.3938320354500604 1106 14-15 -0.10513451084718639 1106 16-17 -0.30658772558022207 1106 18-19 -0.2748007318614327 1106 20-21 -0.2825280954649543 1106 22-23 -0.1669265761568397 1106 24-25 0.026525373634626703 1106 26-27 0.035599691125732136 1106 28-29 -0.06907502814183886 1106 30-31 -0.17902222492663356 1106 32-33 -0.10152557309206856 1106 34-35 -0.4936730824301563 1106 36-37 0.04172410211655375 1106 38-39 0.04461137497554546 1106 40-41 -0.016211437342867896 1106 42-43 0.13128210738289425 1106 44-45 -0.07118903374521324 1106 46-47 0.1879841683243697 1106 48-49 0.29745204080440857 1106 50-51 0.3268021930178051 1106 52-53 0.1381479412508213 1106 54-55 0.019562777480615523 1106 56-57 -0.10375453398345513 1106 58-59 -0.11467465645971231 1106 60-61 -0.16523316567034385 1106 62-63 -0.13654281439149685 1106 64-65 -0.263493786770546 1106 66-67 -0.32572543824775124 1106 68-69 -0.025685713956143275 1106 70-71 0.13716886576204956 1106 72-73 -0.053138280962869544 1106 74-75 -0.20335380870876563 1106 76-77 -0.1898102484441715 1106 78-79 0.03413227787842388 1106 80-81 -0.25642995085819464 1106 82-83 -0.15461670880905842 1106 84-85 -0.06639429772572925 1106 86-87 0.005551899118536596 1106 88-89 0.24865581219725286 1106 90-91 0.08500696866104462 1106 92-93 -0.053024956648059174 1106 94-95 -0.2832095231681784 1106 96-97 -0.1518733427534258 1106 98-99 0.02195315374978435 1106 100-101 0.7048522383223847 1107 1 0.15391734728522977 1107 2 0.09416975945859463 1107 3 0.07672599877733433 1107 4 0.05763331534076599 1107 5 -0.03285424382879398 1107 6 0.11534381186748277 1107 7 0.07846928414642207 1107 8 0.05432376482904999 1107 9 0.14671438604592169 1107 10-11 0.14953607104064304 1107 12-13 0.09641841733935763 1107 14-15 0.11916013522564839 1107 16-17 0.11446011592922645 1107 18-19 0.09887069719118813 1107 20-21 -0.009552757265595346 1107 22-23 -0.005901315819116348 1107 24-25 -0.10538079855231075 1107 26-27 -0.11794612287174999 1107 28-29 -0.11218524447023981 1107 30-31 0.020380663079571093 1107 32-33 -0.014642798263786005 1107 34-35 0.054188831696613704 1107 36-37 0.06058061127209413 1107 38-39 0.1014380661019132 1107 40-41 0.056061267552443894 1107 42-43 -0.15408793622480488 1107 44-45 -0.23503920773553943 1107 46-47 -0.3260969995225267 1107 48-49 -0.4022118974659463 1107 50-51 -0.23662427631300176 1107 52-53 -0.07487555197741358 1107 54-55 0.014306792757565745 1107 56-57 0.10168910895678351 1107 58-59 0.09205188982586776 1107 60-61 0.11302082911124245 1107 62-63 0.09867999168508135 1107 64-65 0.3633808520991195 1107 66-67 0.2221134801361444 1107 68-69 0.2535159645387566 1107 70-71 0.23749249035752484 1107 72-73 0.2924284636949892 1107 74-75 0.3513854519554265 1107 76-77 0.21722962293513248 1107 78-79 0.33054351690200434 1107 80-81 0.2958367816216416 1107 82-83 0.2839820666514399 1107 84-85 0.4076697431509828 1107 86-87 0.6313603128398064 1107 88-89 0.851458766534833 1107 90-91 0.7813776574450806 1107 92-93 0.5871862414366227 1107 94-95 0.9613822667686271 1107 96-97 0.7869285077884953 1107 98-99 0.9530452766390347 1107 100-101 1.453345855222377 1108 1 -0.013967735307524265 1108 2 -0.010926365634254864 1108 3 0.010570492616807314 1108 4 -0.05392512245938974 1108 5 -0.05225358429945004 1108 6 0.0016576822408893577 1108 7 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72-73 -0.8685011476730651 2316 74-75 -0.8141890901077247 2316 76-77 -0.5068333584695175 2316 78-79 -0.39197636451288886 2316 80-81 -0.5273944714936505 2316 82-83 -0.3190001021638693 2316 84-85 -0.2674855654776991 2316 86-87 -0.29503199406428493 2316 88-89 -0.24261562529967406 2316 90-91 0.1818837456127973 2316 92-93 0.30657216232083684 2316 94-95 0.27773860563581465 2316 96-97 0.7993662236878905 2316 98-99 0.7232050052896319 2316 100-101 1.9084049133148397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 10.0 5 17.0 6 32.0 7 84.0 8 194.0 9 395.0 10 741.0 11 1255.0 12 2007.0 13 2998.0 14 3956.0 15 5699.0 16 8009.0 17 11776.0 18 10798.0 19 6171.0 20 5215.0 21 5303.0 22 5398.0 23 5949.0 24 6602.0 25 7904.0 26 9486.0 27 11598.0 28 14490.0 29 18121.0 30 22558.0 31 29015.0 32 36990.0 33 49355.0 34 68541.0 35 99323.0 36 150571.0 37 218138.0 38 159046.0 39 13315.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61674959093052 19.89889599187615 15.436228061409379 21.048126355783957 2 15.812686352560124 21.27751459288745 39.79254640210279 23.117252652449636 3 19.645230131222473 25.471084136761966 30.230711406416326 24.652974325599228 4 12.888155670919668 17.242461392542367 37.230050501228476 32.63933243530949 5 13.160029625025476 38.57024945159396 33.66960831599489 14.600112607385674 6 31.156382275632776 37.284638242698506 17.06638817837377 14.49259130329494 7 28.228017334887216 34.016134158708056 19.992735088011383 17.763113418393345 8 27.09035229778067 34.89478490310928 19.116001473162708 18.89886132594734 9 26.97592993395993 16.667019822110557 20.046919223259827 36.31013102066969 10-11 24.96879619399333 27.64011442236382 26.548107339074633 20.842982044568217 12-13 26.27605233575449 24.75312466573695 27.677030755227623 21.293792243280933 14-15 23.064077532755167 25.773687901399 26.729881491123187 24.432353074722645 16-17 21.503281823089303 29.357912952228162 27.232421687780317 21.906383536902222 18-19 21.411764112343793 27.331052958181353 30.541589098595956 20.715593830878902 20-21 22.572950600087076 26.04911183883592 30.919026965449774 20.458910595627227 22-23 22.124324648847672 26.04896683697279 30.807969458916908 21.018739055262632 24-25 21.905032417163564 26.17523884671482 31.01291591011483 20.906812826006785 26-27 21.63721801286922 26.377003310640163 30.963869523847006 21.02190915264362 28-29 21.453567042806934 26.778425700104236 30.881627315391746 20.88637994169708 30-31 21.676955272313393 26.44465947708725 30.798313731504727 21.080071519094627 32-33 21.591239314514503 26.35652187951535 30.8369590662157 21.215279739754443 34-35 21.87959417394419 26.571011992149856 30.655961011638993 20.893432822266956 36-37 22.077008067079355 26.272494740506424 30.67528365949761 20.97521353291661 38-39 21.823806624089553 26.635854679488567 30.575608348397104 20.964730348024776 40-41 21.897201495361053 26.64169353170579 30.42938656899396 21.0317184039392 42-43 21.792938301081115 26.648405015109 30.201253222236296 21.357403461573586 44-45 21.91596918466498 26.863929207468733 30.007769419765605 21.21233218810068 46-47 22.187898876461183 27.19993138694233 29.391462719397822 21.22070701719867 48-49 22.268973276223054 27.43225721824502 28.96712122817373 21.331648277358195 50-51 22.183661011134486 27.666399277544862 28.867834097662616 21.282105613658032 52-53 22.386775842149607 27.720028454238623 28.53773465917977 21.355461044432 54-55 22.27038589799862 27.72850418489201 28.484307285596806 21.516802631512565 56-57 22.24939837447594 27.964159767522816 28.382043559201463 21.404398298799777 58-59 22.3591792667484 27.49032606337627 28.497878544797768 21.652616125077568 60-61 22.236079369163477 27.60812862930282 28.91016230015186 21.245629701381848 62-63 22.173722207927835 27.559342727268145 28.940634569881894 21.32630049492213 64-65 22.117469590793743 27.62008546361742 28.8123886929717 21.450056252617134 66-67 22.15672029584336 27.675682220641427 28.54343559705973 21.62416188645548 68-69 22.20999631709323 27.739098848208744 28.589446706320977 21.461458128377046 70-71 22.311906888044682 27.599047489317048 28.378461553984856 21.710584068653418 72-73 22.26668624159804 27.696106253534715 28.274525295301462 21.762682209565785 74-75 22.08881354906086 28.088672286883305 28.02737459197934 21.7951395720765 76-77 22.18028080902867 27.78758204557723 28.267570744603027 21.764566400791068 78-79 22.180633964472563 27.890400730527258 28.106330059077862 21.822635245922317 80-81 22.121606554565037 27.94529117666349 28.125501354603383 21.807600914168095 82-83 22.170997633343273 27.907630260715667 28.15186358451376 21.7695085214273 84-85 22.078846572976115 27.85788808795396 28.05737066425579 22.00589467481414 86-87 22.105411854923744 27.87319701533199 28.019403369102935 22.00198776064133 88-89 22.167163606827 27.725123982786194 27.974703980061854 22.133008430324956 90-91 22.147692320112377 27.58064971865439 28.10403531149844 22.1676226497348 92-93 22.102937600335807 27.595411721139364 28.062241245874315 22.23940943265052 94-95 22.00632652752342 27.607371867637337 28.06723070636134 22.3190708984779 96-97 21.800184649846376 27.624676484388004 28.070913613133346 22.50422525263227 98-99 21.69853061739295 27.716516365012296 27.877908809989275 22.707044207605474 100-101 21.766834018719884 27.749526957136013 28.1647450614325 22.31889396271161 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 385.0 1 278.0 2 211.5 3 286.0 4 268.0 5 246.0 6 452.0 7 1049.5 8 1808.5 9 2144.5 10 1951.5 11 1719.5 12 1723.0 13 1827.0 14 1943.0 15 2022.5 16 2135.5 17 2217.0 18 2269.0 19 2447.0 20 2630.0 21 2841.0 22 3223.0 23 3785.5 24 4426.5 25 5259.5 26 6537.5 27 8017.0 28 9371.0 29 10801.5 30 12343.5 31 14199.5 32 16250.5 33 18116.5 34 19810.5 35 21588.0 36 24100.5 37 26145.0 38 27347.0 39 28654.0 40 29951.5 41 31123.0 42 32178.0 43 33507.0 44 35366.0 45 36520.0 46 36721.5 47 38022.0 48 40291.0 49 39274.5 50 36161.5 51 33957.5 52 32504.0 53 32066.5 54 31371.0 55 28921.5 56 25551.0 57 22664.5 58 20595.5 59 18457.5 60 15270.5 61 12336.5 62 9881.5 63 7367.5 64 5129.0 65 3594.5 66 2588.5 67 1985.5 68 1512.5 69 1141.0 70 823.5 71 494.5 72 337.0 73 272.5 74 186.5 75 119.5 76 76.0 77 37.5 78 28.5 79 15.5 80 8.0 81 6.5 82 3.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04056242526978553 2 0.0 3 0.0 4 0.0 5 0.0011099171093722408 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00797122287640064 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.045077769873822E-5 22-23 6.558601100835969E-4 24-25 5.045077769873822E-5 26-27 0.001816227997154576 28-29 0.003430652883514199 30-31 1.0090155539747644E-4 32-33 1.0090155539747644E-4 34-35 0.0 36-37 0.0 38-39 5.045077769873822E-5 40-41 0.0 42-43 0.010039704762048908 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.5135233309621467E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.54056999288644E-4 84-85 1.0090155539747644E-4 86-87 0.0 88-89 0.0 90-91 0.01140187575991484 92-93 0.001816227997154576 94-95 0.0 96-97 0.0 98-99 2.5225388849369114E-4 100-101 0.014126217755646703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 991065.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.56849552739312 #Duplication Level Percentage of deduplicated Percentage of total 1 80.28638516484658 50.23398331097264 2 11.755904170215258 14.710984749891509 3 3.4406876743870094 6.458359540981206 4 1.4673242511552809 3.6723308338257863 5 0.7997963030692175 2.502102570570594 6 0.5039324471558388 1.8918177039587023 7 0.33904891439053597 1.484964633852822 8 0.2382789739065239 1.192700553051373 9 0.1846980926678249 1.0400653606504358 >10 0.9106164107752455 10.115626455433622 >50 0.051045093724233805 2.1741541247115928 >100 0.021313699350542146 2.563275509059282 >500 1.6146739266946687E-4 0.10102771836059367 >1k 4.8440217800840065E-4 0.6378890137287884 >5k 3.2293478533893373E-4 1.2207179209510532 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6771 0.683204431596313 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5312 0.5359890622713949 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3786 0.38201328873484586 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1348 0.13601529667579826 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1180 0.11906383536902222 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1000 0.10090155539747646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.0450777698738226E-5 0.0 18-19 0.0 0.0 0.0 1.5135233309621467E-4 0.0 20-21 0.0 0.0 0.0 2.018031107949529E-4 0.0 22-23 0.0 0.0 0.0 3.0270466619242934E-4 0.0 24-25 0.0 0.0 0.0 5.549585546861205E-4 0.0 26-27 0.0 0.0 0.0 9.08113998577288E-4 0.0 28-29 0.0 0.0 0.0 0.0016648756640583614 0.0 30-31 0.0 0.0 0.0 0.003733357549706629 0.0 32-33 0.0 5.0450777698738226E-5 0.0 0.005499134769162467 0.0 34-35 0.0 1.0090155539747645E-4 0.0 0.00711355965552209 0.0 36-37 0.0 1.0090155539747645E-4 0.0 0.009636098540459001 0.0 38-39 0.0 1.0090155539747645E-4 0.0 0.014328020866441657 0.0 40-41 0.0 1.0090155539747645E-4 0.0 0.01957490174711043 0.0 42-43 0.0 1.0090155539747645E-4 0.0 0.024720881072381733 0.0 44-45 0.0 1.0090155539747645E-4 0.0 0.027495673845812335 0.0 46-47 0.0 1.0090155539747645E-4 0.0 0.03067407284083284 0.0 48-49 0.0 1.0090155539747645E-4 0.0 0.037131772386271335 0.0 50-51 0.0 1.0090155539747645E-4 0.0 0.044800290596479546 0.0 52-53 0.0 2.018031107949529E-4 0.0 0.06442564312128872 0.0 54-55 0.0 4.036062215899058E-4 0.0 0.07562571577040861 0.0 56-57 0.0 4.036062215899058E-4 0.0 0.10463491294718308 0.0 58-59 0.0 4.036062215899058E-4 0.0 0.15740642642006325 0.0 60-61 0.0 4.036062215899058E-4 0.0 0.20467880512378098 0.0 62-63 0.0 4.036062215899058E-4 0.0 0.2278357120875018 0.0 64-65 0.0 4.036062215899058E-4 0.0 0.26991166068824946 0.0 66-67 0.0 4.036062215899058E-4 0.0 0.36395191031869756 0.0 68-69 0.0 4.036062215899058E-4 0.0 0.5062231034291393 0.0 70-71 0.0 4.036062215899058E-4 0.0 0.6374960270012562 0.0 72-73 0.0 4.036062215899058E-4 0.0 0.7961637228637879 0.0 74-75 0.0 4.036062215899058E-4 0.0 0.9799054552425925 0.0 76-77 0.0 4.036062215899058E-4 0.0 1.0949332283957158 0.0 78-79 0.0 4.036062215899058E-4 0.0 1.2735794322269478 0.0 80-81 0.0 4.036062215899058E-4 0.0 1.535065813039508 0.0 82-83 0.0 4.036062215899058E-4 0.0 1.7701159863379294 0.0 84-85 0.0 4.036062215899058E-4 0.0 2.0445682170190653 0.0 86-87 0.0 4.036062215899058E-4 0.0 2.3730027798378512 0.0 88-89 0.0 4.036062215899058E-4 0.0 2.831903053785574 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5060 0.0 33.62216 1 GTATCAA 7460 0.0 31.532583 1 TATCAAC 9175 0.0 25.625546 2 ATCAACG 9230 0.0 25.318468 3 TCAACGC 9240 0.0 25.291067 4 CAACGCA 9355 0.0 25.081709 5 AACGCAG 9655 0.0 24.351566 6 GAGTACT 5765 0.0 23.113905 12-13 TACCTGG 840 0.0 22.052576 2 GTACTTT 6000 0.0 21.928177 14-15 AGTACTT 5855 0.0 21.784979 12-13 GTGTACG 110 0.0028694274 21.600834 1 ACGCAGA 10915 0.0 21.543745 7 CGCAGAG 10920 0.0 21.53388 8 GTACATG 5345 0.0 21.427055 1 TACATGG 5460 0.0 21.139156 2 TGCACCG 160 4.990132E-5 20.780312 5 ACATGGG 5465 0.0 20.772165 3 GCAGAGT 11375 0.0 20.505474 9 AGAGTAC 9290 0.0 20.045185 10-11 >>END_MODULE