FastQCFastQC Report
Thu 26 May 2016
SRR937342_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937342_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968877
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58260.6013147179672962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.4431935116635032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.32852467341055674No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14750.15223810659144557No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.10114803014211297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58400.038.5289341
GGTATCA36950.034.245691
TATCAAC72750.030.8540882
ATCAACG73600.030.4977573
TCAACGC73750.030.372824
CAACGCA75050.030.0352575
AACGCAG77050.029.3456216
ACGCAGA86950.025.949747
CGCAGAG86600.025.9449148
GGACCGA2101.2641976E-924.8818326
GTGTACG1351.327075E-524.666241
GTACATG53900.024.4472331
GCAGAGT90750.024.1315789
TACATGG53150.024.0687072
GAGTACT45600.023.54249812-13
ACATGGG54000.022.7211153
AGTACTT47400.022.64848112-13
GTACTTT48300.022.07836714-15
AGAGTAC76600.021.73543210-11
ACCGCAT1100.002876446621.5916758
Produced by FastQC (version 0.11.5)