FastQCFastQC Report
Thu 26 May 2016
SRR937342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937342_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968877
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69360.7158803439445873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55870.5766469840856993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38650.39891544540741497No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.13231813738998863No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11870.12251297120274297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.10496688434135602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47000.035.7985341
GTATCAA68900.031.8700391
TATAACG700.00838697327.1454832
GTACATG52700.026.876051
TACATGG52750.025.8461022
ATCAACG86650.025.0543563
TCAACGC87200.024.896334
TATCAAC87500.024.8652612
CAACGCA88550.024.516775
AACGCAG90350.024.0246146
ACATGGG52750.023.9549243
TAAGGTG4000.023.7522985
ATAACGT1000.001652527823.7522983
GAGTACT55300.022.67850512-13
GTACTTT57050.021.9794414-15
AGTACTT56650.021.92842312-13
CGCAGAG100150.021.629658
ACGCAGA100400.021.5284777
GTATAGG2657.858034E-1021.5226231
CATGGGA31650.021.3132784
Produced by FastQC (version 0.11.5)