FastQCFastQC Report
Thu 26 May 2016
SRR937341_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937341_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090821
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64020.5868973919644012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47530.43572685160993413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34650.3176506502900109No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17250.1581377696248972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63500.034.750121
GGTATCA42250.033.4304121
GTACATG60900.027.7219051
TACATGG60100.027.2920882
TATCAAC80850.027.2853832
ATCAACG80700.027.2182753
TCAACGC81050.027.1593974
CAACGCA81600.026.9738565
AACGCAG84450.026.1006326
ACATGGG61000.025.7982543
GTAAGGT3150.025.6584554
CTATCGG1151.2713452E-424.812211
CATGGGA36500.023.967194
GTTCGAC601.7125897E-423.7477716-17
GTACCTG10350.023.4337521
CGCAGAG92400.023.3408478
TACCTGG9600.023.2765582
ACGCAGA93050.023.228857
GAGTACT49250.022.61618612-13
AGTACTT50300.022.04964812-13
Produced by FastQC (version 0.11.5)