##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937341_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1090821 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52824982283986 31.0 31.0 34.0 30.0 34.0 2 31.621860965272944 33.0 31.0 34.0 30.0 34.0 3 31.65747817469594 33.0 31.0 34.0 30.0 34.0 4 35.29974212084292 37.0 35.0 37.0 33.0 37.0 5 35.296302509761 37.0 35.0 37.0 33.0 37.0 6 35.346902012337495 37.0 35.0 37.0 33.0 37.0 7 35.28884849118233 37.0 35.0 37.0 33.0 37.0 8 35.27291095422622 37.0 35.0 37.0 33.0 37.0 9 36.86525836961334 39.0 37.0 39.0 33.0 39.0 10-11 36.760302102728126 39.0 37.0 39.0 33.0 39.0 12-13 36.6413206199734 39.0 37.0 39.0 32.0 39.0 14-15 37.753370167974396 40.0 37.5 41.0 32.5 41.0 16-17 37.69482069010406 40.0 37.0 41.0 32.0 41.0 18-19 37.67780414935173 40.0 37.0 41.0 32.0 41.0 20-21 37.61355162762726 40.0 37.0 41.0 32.0 41.0 22-23 37.47410528400168 40.0 37.0 41.0 32.0 41.0 24-25 37.3219597899197 40.0 37.0 41.0 32.0 41.0 26-27 37.136441267632364 39.0 36.5 41.0 31.0 41.0 28-29 36.95466579759649 39.0 36.0 41.0 31.0 41.0 30-31 36.92311295803803 39.0 36.0 41.0 31.0 41.0 32-33 36.75150047533005 39.0 36.0 41.0 30.0 41.0 34-35 36.54050618754131 39.0 35.0 40.5 30.0 41.0 36-37 36.5384760652756 39.0 35.0 41.0 30.0 41.0 38-39 36.50515941662289 39.0 35.0 41.0 30.0 41.0 40-41 36.329276755764695 39.0 35.0 41.0 30.0 41.0 42-43 36.073126571637324 39.0 35.0 41.0 29.5 41.0 44-45 35.817222532386154 39.0 35.0 41.0 28.5 41.0 46-47 35.451540628572424 39.0 35.0 40.5 26.5 41.0 48-49 35.09619268422591 39.0 35.0 40.0 25.5 41.0 50-51 34.16280993856921 38.0 33.5 39.5 23.5 40.5 52-53 34.294917314573155 38.0 33.5 39.5 24.0 40.5 54-55 34.64320956417231 38.0 34.0 40.0 24.0 41.0 56-57 34.41513960585651 38.0 34.0 40.0 23.0 41.0 58-59 34.1576033098006 38.0 34.0 40.0 22.5 41.0 60-61 33.87308550165426 38.0 33.5 40.0 22.0 41.0 62-63 33.55167208918787 37.0 33.0 40.0 21.0 41.0 64-65 33.18259136925307 37.0 33.0 40.0 19.5 41.0 66-67 32.79315213036786 36.0 32.0 39.0 18.5 41.0 68-69 32.28855559253076 35.5 31.5 39.0 15.0 40.5 70-71 31.852246610580472 35.0 31.0 38.5 12.0 40.0 72-73 31.44415353206438 35.0 31.0 38.0 8.0 40.0 74-75 30.986595875950314 35.0 30.5 37.0 7.0 39.0 76-77 30.54611159851158 35.0 30.0 36.5 4.0 39.0 78-79 30.081112758188556 34.0 30.0 36.0 2.0 39.0 80-81 29.63052141460423 34.0 29.0 36.0 2.0 37.5 82-83 29.175416498215565 34.0 29.0 35.0 2.0 37.0 84-85 28.716285256701145 34.0 29.0 35.0 2.0 36.5 86-87 28.2826471987613 34.0 27.5 35.0 2.0 36.0 88-89 27.8831430638024 34.0 27.0 35.0 2.0 36.0 90-91 27.436408448315532 33.0 26.0 35.0 2.0 35.0 92-93 26.97127484711057 33.0 25.5 35.0 2.0 35.0 94-95 26.417971876229004 33.0 25.0 35.0 2.0 35.0 96-97 25.777638127612136 32.0 23.5 35.0 2.0 35.0 98-99 25.063032339861444 32.0 19.0 34.0 2.0 35.0 100-101 23.234001270602604 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0484685519989867 1101 2 -0.06980751328237034 1101 3 0.06326759185472497 1101 4 0.0018581536485342554 1101 5 0.06352972439719196 1101 6 -0.1177410295268615 1101 7 -0.005483987891700792 1101 8 -0.0635470808828984 1101 9 -0.01688815797083265 1101 10-11 0.13400144408732473 1101 12-13 0.08292764879689685 1101 14-15 0.12978919360978125 1101 16-17 0.0641529292268288 1101 18-19 0.07867058642746372 1101 20-21 0.04372392437983308 1101 22-23 0.03275075448813425 1101 24-25 0.08901761657498497 1101 26-27 0.20360474189362066 1101 28-29 0.10401749359353829 1101 30-31 -0.14240976501015723 1101 32-33 0.021093198071461927 1101 34-35 -0.15333381523688416 1101 36-37 -0.07013426340715512 1101 38-39 0.2411961099464932 1101 40-41 0.318482295447474 1101 42-43 0.35854381741484787 1101 44-45 0.17236025572884017 1101 46-47 0.12926924748065716 1101 48-49 0.3187702978962008 1101 50-51 0.3284215949078657 1101 52-53 0.3401192358942282 1101 54-55 0.4341581985217715 1101 56-57 0.5784593224688948 1101 58-59 0.49647258957667617 1101 60-61 0.4302735958716042 1101 62-63 0.4085931705607422 1101 64-65 0.20520977392635587 1101 66-67 0.2947783855461452 1101 68-69 0.2958608645495815 1101 70-71 0.47377047394517646 1101 72-73 0.5862926605575431 1101 74-75 0.5619744007722147 1101 76-77 0.6167167841632839 1101 78-79 0.5845455070675349 1101 80-81 0.7843097201747788 1101 82-83 0.5773816014244915 1101 84-85 0.5476382107735773 1101 86-87 0.6633335376985379 1101 88-89 0.7334426429099885 1101 90-91 0.8334693540269775 1101 92-93 0.7755788517093798 1101 94-95 0.8166220878721688 1101 96-97 0.8029297276743534 1101 98-99 0.8410605829248645 1101 100-101 1.2906603054513752 1102 1 -0.08005914147513593 1102 2 -0.1835918652101789 1102 3 -0.1137363926759214 1102 4 -0.3224086634146275 1102 5 -0.05867414587117992 1102 6 -0.07161679127536758 1102 7 -0.07184583308097103 1102 8 0.08972184563766916 1102 9 0.10959322313780007 1102 10-11 -0.17337323363445734 1102 12-13 0.04565599023601408 1102 14-15 0.17909135140892118 1102 16-17 0.007298291350352315 1102 18-19 -0.03045263371785012 1102 20-21 0.1789635821789659 1102 22-23 0.04151360899417256 1102 24-25 0.1579338828353869 1102 26-27 0.19254565511288746 1102 28-29 -0.021559311164459416 1102 30-31 0.06810657596019354 1102 32-33 0.015154855753401364 1102 34-35 0.011288093822059864 1102 36-37 -0.013384915288092714 1102 38-39 0.1856589673823308 1102 40-41 0.19793101658892454 1102 42-43 0.12584246677615596 1102 44-45 0.11497825350218704 1102 46-47 0.014708498037322215 1102 48-49 0.0757468887786601 1102 50-51 0.05556364635518207 1102 52-53 0.003918053719139891 1102 54-55 0.07664911613574787 1102 56-57 0.21073621805074794 1102 58-59 0.02262381862858831 1102 60-61 0.15978604020536125 1102 62-63 0.2082871893700613 1102 64-65 0.18896768439160638 1102 66-67 0.10235998053033057 1102 68-69 0.1453203160906611 1102 70-71 0.17809303049035918 1102 72-73 0.20053754072864294 1102 74-75 0.056708997338486 1102 76-77 0.0865041171645693 1102 78-79 0.06573709414125162 1102 80-81 0.1981955164949447 1102 82-83 0.1982487495552796 1102 84-85 0.1747266102227769 1102 86-87 0.10818550945524663 1102 88-89 0.2598062826029448 1102 90-91 0.19424241889279514 1102 92-93 0.1463251455099197 1102 94-95 0.2535764122598394 1102 96-97 0.4617945759106128 1102 98-99 0.4751603646546201 1102 100-101 0.5339620457537322 1103 1 -0.08485669216070946 1103 2 -0.05565656988614265 1103 3 0.06865842743424011 1103 4 -0.10393699459944372 1103 5 -0.27541906266480254 1103 6 -0.07933132602281745 1103 7 -0.10790986390248491 1103 8 -0.11341230999340723 1103 9 0.045106451193582586 1103 10-11 -0.11060772033316368 1103 12-13 0.17423492642492988 1103 14-15 -0.004307841430652104 1103 16-17 -0.06421634301036505 1103 18-19 -0.02173372624100267 1103 20-21 -0.025683083706418586 1103 22-23 -0.0531656875603872 1103 24-25 -0.08416297641692694 1103 26-27 -0.2394545572977549 1103 28-29 -0.17630595654123482 1103 30-31 -0.2667359105627156 1103 32-33 -0.31718173454309095 1103 34-35 -0.053266429792145686 1103 36-37 -0.09001296960661875 1103 38-39 -0.1469440517785685 1103 40-41 -0.28933441614282174 1103 42-43 -0.16739957881156187 1103 44-45 -0.29259931300431674 1103 46-47 0.040994314866104276 1103 48-49 0.06978107956735613 1103 50-51 -0.1365379738252912 1103 52-53 -0.10833089887666603 1103 54-55 0.03725792897999014 1103 56-57 0.001976842684527469 1103 58-59 -0.21242228912952044 1103 60-61 -0.03199055507179338 1103 62-63 -0.09949308280853586 1103 64-65 0.06673268497757334 1103 66-67 0.20212339902323606 1103 68-69 0.19646733355227752 1103 70-71 0.09708583782658309 1103 72-73 0.4575864610966285 1103 74-75 0.466960923683267 1103 76-77 0.3033744661039819 1103 78-79 0.4022678790351897 1103 80-81 0.2698218495548339 1103 82-83 0.41161340735441243 1103 84-85 0.3697406366495919 1103 86-87 0.6481718126311513 1103 88-89 0.6616771442576983 1103 90-91 0.6586041249164651 1103 92-93 0.7968052668037231 1103 94-95 0.6454630582225711 1103 96-97 0.7382397007201753 1103 98-99 0.7325700168871307 1103 100-101 1.0996225696023174 1104 1 -0.07860974143324384 1104 2 -0.06924589061489073 1104 3 -0.1639660524502986 1104 4 -0.27377521387993653 1104 5 -0.10052206546781406 1104 6 -0.11259413575006505 1104 7 -2.7773185112778265E-4 1104 8 -0.0644092250107633 1104 9 -0.15639272422747297 1104 10-11 -0.0802174876516375 1104 12-13 0.03393244786491323 1104 14-15 0.07253730468399766 1104 16-17 0.04643723551384937 1104 18-19 -0.01395448707971525 1104 20-21 -0.0030324380490469593 1104 22-23 0.013300569630338543 1104 24-25 0.08163559443656965 1104 26-27 0.15123331220915048 1104 28-29 0.05493394491740844 1104 30-31 -0.024562685911796223 1104 32-33 -0.20676746014473935 1104 34-35 0.019729212646680594 1104 36-37 -0.02725824520520348 1104 38-39 0.03651780703940943 1104 40-41 -0.21420845683936562 1104 42-43 -0.23262548932665794 1104 44-45 -0.007265548676208766 1104 46-47 0.05952067449383236 1104 48-49 -0.08107165007648831 1104 50-51 -0.09599076505748627 1104 52-53 -0.017504780222630245 1104 54-55 0.10114865232772985 1104 56-57 0.18614223708184596 1104 58-59 0.07945222275498054 1104 60-61 0.1579698484431944 1104 62-63 0.10543519088118103 1104 64-65 0.13609076557297328 1104 66-67 0.16226882729414172 1104 68-69 0.2979561669539379 1104 70-71 0.27964033516733977 1104 72-73 0.15005283330173214 1104 74-75 0.25939520316451237 1104 76-77 0.23072433059106245 1104 78-79 0.1405637739491432 1104 80-81 0.22351232729119985 1104 82-83 0.24734086407097422 1104 84-85 0.4284765821698713 1104 86-87 0.27322027635431567 1104 88-89 0.18856569902773046 1104 90-91 0.42694242674760474 1104 92-93 0.38809863140117784 1104 94-95 0.324599819602966 1104 96-97 0.16171733691483325 1104 98-99 0.4243217230884433 1104 100-101 0.8052476542399205 1105 1 0.18701777779835638 1105 2 0.12845231763584763 1105 3 0.24233457676733394 1105 4 0.272964766720186 1105 5 0.17618602899779745 1105 6 0.2194876150601317 1105 7 0.18070725802889598 1105 8 0.15321794775719155 1105 9 0.1688458478205348 1105 10-11 0.21776681399714448 1105 12-13 0.278338286194753 1105 14-15 0.40452435195280856 1105 16-17 0.45242975210783953 1105 18-19 0.47137037737420684 1105 20-21 0.47937644436046156 1105 22-23 0.4964430783714988 1105 24-25 0.42638700938052665 1105 26-27 0.33100359220291153 1105 28-29 0.24310048957390507 1105 30-31 0.2995733595083365 1105 32-33 0.07174365408547345 1105 34-35 0.26427227979957735 1105 36-37 0.15890804586495477 1105 38-39 0.10685436203242915 1105 40-41 0.018855393175272184 1105 42-43 0.010836966067451215 1105 44-45 -0.013982977832483812 1105 46-47 0.25828018854725343 1105 48-49 0.05546946352236404 1105 50-51 -0.024417585309123524 1105 52-53 -0.0645145224255046 1105 54-55 0.1220969191485679 1105 56-57 -0.08859195381857887 1105 58-59 0.08455134318606383 1105 60-61 0.18924393197119116 1105 62-63 0.29330275410949014 1105 64-65 -0.019763609078417232 1105 66-67 0.06312674224044912 1105 68-69 0.19279740677200152 1105 70-71 -0.0706847614798427 1105 72-73 0.010433202379434903 1105 74-75 0.16777670137975775 1105 76-77 0.32150876289420793 1105 78-79 0.412875850033398 1105 80-81 0.43322823232050567 1105 82-83 0.5279889389279937 1105 84-85 0.5442410954223682 1105 86-87 0.43545553038678264 1105 88-89 0.2055617235661238 1105 90-91 0.20973685431818367 1105 92-93 0.38202690820893537 1105 94-95 0.5808036842379494 1105 96-97 0.43564354746350276 1105 98-99 0.5084636020380131 1105 100-101 0.6401123325322544 1106 1 -0.04575824239823589 1106 2 -0.09686965680964477 1106 3 -0.0986845756483632 1106 4 -0.01666353627417294 1106 5 0.039401267635568615 1106 6 0.038019867555831866 1106 7 -0.03630259074199671 1106 8 -0.06888951807668064 1106 9 -0.03654162552902562 1106 10-11 -0.0982959155945835 1106 12-13 -0.26737242119461513 1106 14-15 -0.36769222885600783 1106 16-17 -0.22762790799077948 1106 18-19 -0.13173953404728422 1106 20-21 -0.18520974749400665 1106 22-23 -0.12062559135927842 1106 24-25 -0.07245242854720146 1106 26-27 0.010100549848822027 1106 28-29 0.09791743551546972 1106 30-31 0.1619490680675355 1106 32-33 0.32293159213856626 1106 34-35 0.2387135853974982 1106 36-37 -0.013629148070080532 1106 38-39 -0.07157659634231806 1106 40-41 0.11090720349054095 1106 42-43 -0.08327387703497635 1106 44-45 -0.05177080709971449 1106 46-47 0.2283910029273386 1106 48-49 0.005200879719261309 1106 50-51 -0.00677817873661013 1106 52-53 0.11526219727975473 1106 54-55 0.3934793553176519 1106 56-57 0.18835902612185862 1106 58-59 0.1124835201659451 1106 60-61 -0.027975350532777554 1106 62-63 0.13044020187820848 1106 64-65 0.1787619176025501 1106 66-67 0.19789226322480857 1106 68-69 0.20849805535835486 1106 70-71 0.3064479471026935 1106 72-73 0.308474757622367 1106 74-75 0.42974628801142245 1106 76-77 0.2813345858341414 1106 78-79 0.49122411200268346 1106 80-81 0.5740727170028244 1106 82-83 0.5522479474208275 1106 84-85 0.4205719719982852 1106 86-87 0.3165758572470203 1106 88-89 0.4990753215000332 1106 90-91 0.4768905242254853 1106 92-93 0.5557198771594862 1106 94-95 0.9714299090513023 1106 96-97 1.1053991622322918 1106 98-99 1.6092918825332099 1106 100-101 1.8497510114721365 1107 1 0.10102017430914856 1107 2 -0.014376745587451722 1107 3 0.14215847734938336 1107 4 0.09359149268645695 1107 5 0.21638059220762784 1107 6 0.2608225552361887 1107 7 -0.011923041500566 1107 8 0.1836505403039439 1107 9 0.017571118402187835 1107 10-11 0.0738893489347987 1107 12-13 0.3042941828318959 1107 14-15 0.25590515362062405 1107 16-17 0.21396432128889842 1107 18-19 0.28880977837258826 1107 20-21 0.3351837997345868 1107 22-23 0.27662367053086 1107 24-25 0.32548169906332447 1107 26-27 0.3798194625827378 1107 28-29 0.19376314027987718 1107 30-31 0.15339451057848663 1107 32-33 0.14030838938908374 1107 34-35 0.3075237982487593 1107 36-37 0.38735764320873045 1107 38-39 0.38018839449133424 1107 40-41 0.45550114911053186 1107 42-43 0.3304915063436695 1107 44-45 0.2528858621978429 1107 46-47 0.07462820867520037 1107 48-49 0.05594567119017313 1107 50-51 -0.1150245466514832 1107 52-53 0.004078005985071798 1107 54-55 0.16946353658991598 1107 56-57 0.29533171083723886 1107 58-59 0.3644577291251565 1107 60-61 0.09250672811820948 1107 62-63 0.33178445965499037 1107 64-65 0.1646929382612754 1107 66-67 0.13506418540067955 1107 68-69 0.31923541353060614 1107 70-71 0.19015440697331698 1107 72-73 0.17854654324269248 1107 74-75 0.2147610919686116 1107 76-77 -0.06447875484897025 1107 78-79 0.0816201230774638 1107 80-81 0.07922800195289881 1107 82-83 0.2691520202838582 1107 84-85 0.5512873643553462 1107 86-87 0.5964312002436394 1107 88-89 0.5661128178982189 1107 90-91 0.5988592626109543 1107 92-93 0.591395534776737 1107 94-95 0.6779649478673164 1107 96-97 0.9138643530968515 1107 98-99 0.8280897761535186 1107 100-101 1.0888819001689285 1108 1 -0.0734085715481676 1108 2 -0.19153820846320002 1108 3 -0.13777058829396793 1108 4 -0.16973777064280426 1108 5 -0.1570132097576007 1108 6 -0.09916930475567654 1108 7 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-1.4965410955999623 2316 92-93 -1.5178505791277601 2316 94-95 -1.666260544261899 2316 96-97 -1.4852162936546058 2316 98-99 -1.5903967005627706 2316 100-101 -1.306164765840471 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4609.0 3 878.0 4 819.0 5 992.0 6 1100.0 7 1326.0 8 1609.0 9 2001.0 10 2590.0 11 3297.0 12 4415.0 13 5400.0 14 6346.0 15 7915.0 16 9874.0 17 12881.0 18 10844.0 19 7652.0 20 7513.0 21 7830.0 22 8481.0 23 9478.0 24 10387.0 25 12013.0 26 13458.0 27 16071.0 28 18805.0 29 22830.0 30 27471.0 31 34085.0 32 42984.0 33 55498.0 34 74967.0 35 103973.0 36 149209.0 37 204353.0 38 164150.0 39 22713.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73033195066563 19.122588766162306 15.06752006831139 22.079559214860673 2 14.928352004120448 20.30452697606828 40.729264389382486 24.037856630428784 3 19.14078954871631 25.13267368986456 29.981126765226897 25.745409996192244 4 12.184564783099773 16.41734760326261 37.96296636951955 33.43512124411807 5 13.16854527747865 38.01908559457871 34.00949221871647 14.802876909226176 6 31.160044839753017 36.46905321964128 17.694244339900028 14.676657600705676 7 28.013908722172907 33.45665194562219 20.455635623824936 18.07380370837997 8 26.690509512174277 34.43367575261639 19.744504184290737 19.131310550918595 9 26.613414537776052 16.476060432865747 20.244752738720294 36.6657722906379 10-11 24.551541742537335 27.0632112372674 27.255547116546996 21.129699903648273 12-13 25.761564117773823 24.31868274759018 28.071837600377503 21.847915534258497 14-15 22.737769464096797 25.369165870032482 26.87885846953443 25.014206196336293 16-17 21.430750555710237 28.883240661357213 27.351281464593974 22.334727318338576 18-19 21.34583236287612 27.141563637416056 30.340098740863347 21.17250525884448 20-21 22.432244326991324 25.95919154888814 30.48942117969179 21.11914294442875 22-23 21.928007952973086 25.959759205731725 30.501985486926657 21.610247354368532 24-25 21.635596786077166 26.155704014831226 30.636399715575546 21.572299483516062 26-27 21.390101133264082 26.279855170807387 30.561651872410277 21.76839182351825 28-29 21.33682812866115 26.647155846124864 30.512641449620276 21.50337457559371 30-31 21.506579412902617 26.284614402017336 30.525507573745575 21.68329861133447 32-33 21.30711854687412 26.25995426242999 30.492080930778386 21.940846259917503 34-35 21.65055684884337 26.434021877008558 30.389093563745305 21.526327710402764 36-37 21.65578058660796 26.213279578240577 30.451224879248578 21.679714955902877 38-39 21.551646526429124 26.454708810197936 30.30961825721567 21.684026406157273 40-41 21.6927255464602 26.50160285192349 30.222635420202593 21.583036181413714 42-43 21.447598031627535 26.47932248888048 30.01983898224505 22.053240497246936 44-45 21.544692288254346 26.745905216971817 29.798258712182125 21.911143782591715 46-47 21.845072085402702 26.97569314124035 29.29424157464674 21.884993198710212 48-49 21.76888219535313 27.114608779629883 29.039663946932798 22.07684507808419 50-51 21.878866882963223 27.309285849178412 28.871041334207586 21.94080593365078 52-53 21.887687601299763 27.441499459351654 28.63161513006579 22.039197809282793 54-55 21.73109901683457 27.358292023475393 28.717979116235554 22.19262984345448 56-57 21.904465763277663 27.58398481725916 28.480315853921233 22.03123356554194 58-59 21.915021324806165 27.260068950283145 28.673104292010464 22.151805432900222 60-61 21.814512573301393 27.345644537852177 28.981285180993808 21.85855770785262 62-63 21.817502907291836 27.27223458216751 29.039298502170595 21.870964008370063 64-65 21.665291920719103 27.319217846100713 28.957247544287334 22.058242688892847 66-67 21.684782908263102 27.460391391661755 28.6590306524603 22.195795047614848 68-69 21.70556485347962 27.41573539434935 28.798268320329505 22.08043143184152 70-71 21.78382127339035 27.310190246098166 28.64499030856429 22.260998171947197 72-73 21.666138375527716 27.41160311094573 28.58133156930031 22.340926944226236 74-75 21.564890668026283 27.794964367491392 28.38823472671026 22.251910237772066 76-77 21.70236517448818 27.425687765728973 28.53773552401581 22.334211535767036 78-79 21.666514305349533 27.50956316096292 28.450972348488158 22.372950185199393 80-81 21.6343764611429 27.640575151775142 28.362775889040865 22.36227249804109 82-83 21.78912968980689 27.5246657865168 28.38588967257139 22.30031485110492 84-85 21.635917229115307 27.532718160875618 28.308695204888068 22.522669405121007 86-87 21.64683198553035 27.562789155295654 28.345612601601932 22.444766257572063 88-89 21.733809955599163 27.35947773665058 28.210287066614114 22.69642524113614 90-91 21.6269995291848 27.193459113719094 28.47779704419921 22.701744312896892 92-93 21.63472933965159 27.164290583434237 28.495038062042138 22.705942014872033 94-95 21.517530228372756 27.158199935599775 28.500499078782788 22.82377075724468 96-97 21.385337138878196 27.08975029097353 28.487479641039254 23.03743292910902 98-99 21.360240598022244 27.18515105592379 28.386625256269326 23.067983089784644 100-101 21.27122139136457 26.91735380053844 28.81498225867341 22.99644254942358 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1734.0 1 1025.5 2 326.0 3 378.0 4 334.5 5 307.5 6 599.0 7 1237.0 8 1984.5 9 2312.0 10 2076.0 11 1764.0 12 1751.5 13 1831.5 14 1941.5 15 2027.5 16 2037.5 17 2114.5 18 2214.5 19 2396.5 20 2615.5 21 2911.0 22 3423.0 23 4077.0 24 4786.0 25 5700.0 26 7123.5 27 8814.5 28 10207.5 29 11587.0 30 13330.0 31 15424.5 32 17830.0 33 19898.0 34 21795.5 35 23719.0 36 26284.0 37 28770.0 38 29986.0 39 30956.0 40 32314.5 41 34111.0 42 35581.5 43 36842.0 44 38232.5 45 39439.0 46 40263.5 47 41649.5 48 43893.5 49 43635.0 50 40483.5 51 37608.5 52 35880.0 53 35418.0 54 34768.0 55 32081.0 56 28505.5 57 25341.0 58 23140.0 59 20542.0 60 17020.5 61 14099.5 62 11343.0 63 8395.0 64 5954.0 65 4312.0 66 3132.5 67 2300.0 68 1672.5 69 1233.5 70 906.5 71 557.5 72 389.0 73 317.5 74 215.0 75 139.5 76 106.0 77 74.0 78 46.5 79 23.0 80 18.5 81 14.0 82 6.5 83 6.0 84 6.5 85 4.5 86 4.0 87 3.5 88 3.5 89 4.0 90 3.0 91 2.5 92 3.0 93 3.0 94 2.5 95 1.5 96 1.5 97 2.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3697215216795423 2 0.3264513609473965 3 0.3270014053634831 4 0.329384931166525 5 0.31205853205979717 6 0.229276847438764 7 0.23679412112528087 8 0.23688579519462863 9 0.23276046207397913 10-11 0.2402318987258221 12-13 0.23986520244843101 14-15 0.21887184056779255 16-17 0.23596905450115097 18-19 0.22153038857887775 20-21 0.22400558845126745 22-23 0.22212627002963825 24-25 0.21149207798529732 26-27 0.22959770668148116 28-29 0.23248543986593584 30-31 0.24330298004897227 32-33 0.2435321652223417 34-35 0.23005607702822 36-37 0.22258464037637707 38-39 0.20965859659834196 40-41 0.2101628039797547 42-43 0.2115379150199712 44-45 0.23340218055941353 46-47 0.22226378113365985 48-49 0.2328979731780008 50-51 0.2433488170836462 52-53 0.2544413794747259 54-55 0.24779500944701283 56-57 0.20319557470932442 58-59 0.1893527902378117 60-61 0.19847436013791445 62-63 0.19975779710878322 64-65 0.19572413805748148 66-67 0.21039198915312413 68-69 0.19865770827661003 70-71 0.20452484871486706 72-73 0.2205678108507262 74-75 0.20887936700888596 76-77 0.2221721070643121 78-79 0.21997192939996574 80-81 0.2020496488424774 82-83 0.21726754435420662 84-85 0.19517409364139487 86-87 0.2014537673917169 88-89 0.2134172334416004 90-91 0.2093377373556248 92-93 0.21405895192703478 94-95 0.21227130757475332 96-97 0.20479987092291035 98-99 0.21694668511148943 100-101 0.19434902701726497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1090821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.53156537857707 #Duplication Level Percentage of deduplicated Percentage of total 1 81.02565335991139 51.47686593777133 2 11.270155643804202 14.32021260222172 3 3.3349551453403743 6.35624762552442 4 1.4323297090250748 3.6399259421041927 5 0.7684458737774245 2.4410284634894115 6 0.45531715025440317 1.735620677962501 7 0.34205671308400704 1.5211978901334438 8 0.21728347836209136 1.1043487608996676 9 0.16845291489601874 0.9631869638335465 >10 0.9168622207837276 10.273444756688535 >50 0.04644048674307994 2.0274365114645847 >100 0.020750405195658513 2.269041201121536 >500 5.763994766786901E-4 0.27785091936664275 >1k 5.763994766786901E-4 1.0074956730246798 >5k 1.4409986916967253E-4 0.5860960743938209 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6402 0.5868973919644012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4753 0.43572685160993413 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3465 0.3176506502900109 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1725 0.1581377696248972 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 9.167406934776649E-5 0.0 0.0 0.0 8 0.0 9.167406934776649E-5 0.0 0.0 0.0 9 0.0 9.167406934776649E-5 0.0 0.0 0.0 10-11 0.0 1.8334813869553298E-4 0.0 0.0 0.0 12-13 0.0 1.8334813869553298E-4 0.0 0.0 0.0 14-15 0.0 1.8334813869553298E-4 0.0 0.0 0.0 16-17 0.0 1.8334813869553298E-4 0.0 4.5837034673883246E-5 0.0 18-19 0.0 1.8334813869553298E-4 0.0 9.167406934776649E-5 0.0 20-21 0.0 4.583703467388325E-4 0.0 9.167406934776649E-5 0.0 22-23 0.0 4.583703467388325E-4 0.0 1.3751110402164974E-4 0.0 24-25 0.0 4.583703467388325E-4 0.0 1.8334813869553298E-4 0.0 26-27 0.0 4.583703467388325E-4 0.0 4.125333120649492E-4 0.0 28-29 0.0 4.583703467388325E-4 0.0 8.250666241298985E-4 0.0 30-31 0.0 5.042073814127158E-4 0.0 0.002566873941737462 0.0 32-33 0.0 5.50044416086599E-4 0.0 0.00407949608597561 0.0 34-35 0.0 5.50044416086599E-4 0.0 0.005592118230213756 0.0 36-37 0.0 5.50044416086599E-4 0.0 0.008113155137277336 0.0 38-39 0.0 5.50044416086599E-4 0.0 0.013155228951404493 0.0 40-41 0.0 5.50044416086599E-4 0.0 0.018013954626836116 0.0 42-43 0.0 5.50044416086599E-4 0.0 0.02186426553944231 0.0 44-45 0.0 5.50044416086599E-4 0.0 0.023926932099767057 0.0 46-47 0.0 5.50044416086599E-4 0.0 0.02667715418020005 0.0 48-49 0.0 5.50044416086599E-4 0.0 0.03071081323150178 0.0 50-51 0.0 5.50044416086599E-4 0.0 0.03657795366975883 0.0 52-53 0.0 5.50044416086599E-4 0.0 0.05129164180007535 0.0 54-55 0.0 5.50044416086599E-4 0.0 0.06009235245746094 0.0 56-57 0.0 5.50044416086599E-4 0.0 0.08548606966679226 0.0 58-59 0.0 6.417184854343654E-4 0.0 0.13040636364719785 0.0 60-61 0.0 6.417184854343654E-4 0.0 0.17124716154162783 0.0 62-63 0.0 6.417184854343654E-4 0.0 0.19173631604085364 0.0 64-65 0.0 7.333925547821319E-4 0.0 0.231247839929741 0.0 66-67 0.0 7.333925547821319E-4 0.0 0.3169630947699027 0.0 68-69 0.0 7.333925547821319E-4 0.0 0.43714779968482453 0.0 70-71 0.0 7.333925547821319E-4 0.0 0.5583867563972458 0.0 72-73 0.0 7.333925547821319E-4 0.0 0.6957145122802 0.0 74-75 0.0 7.333925547821319E-4 0.0 0.8551357188759658 0.0 76-77 0.0 7.333925547821319E-4 0.0 0.9529519508700328 0.0 78-79 0.0 7.333925547821319E-4 0.0 1.1159026091356878 0.0 80-81 0.0 7.333925547821319E-4 0.0 1.3308783017562003 0.0 82-83 0.0 7.333925547821319E-4 0.0 1.5297193581715056 0.0 84-85 0.0 7.333925547821319E-4 0.0 1.7809979822537336 0.0 86-87 0.0 7.333925547821319E-4 0.0 2.0810930482636474 0.0 88-89 0.0 7.333925547821319E-4 0.0 2.5065065670719577 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6350 0.0 34.75012 1 GGTATCA 4225 0.0 33.430412 1 GTACATG 6090 0.0 27.721905 1 TACATGG 6010 0.0 27.292088 2 TATCAAC 8085 0.0 27.285383 2 ATCAACG 8070 0.0 27.218275 3 TCAACGC 8105 0.0 27.159397 4 CAACGCA 8160 0.0 26.973856 5 AACGCAG 8445 0.0 26.100632 6 ACATGGG 6100 0.0 25.798254 3 GTAAGGT 315 0.0 25.658455 4 CTATCGG 115 1.2713452E-4 24.81221 1 CATGGGA 3650 0.0 23.96719 4 GTTCGAC 60 1.7125897E-4 23.74777 16-17 GTACCTG 1035 0.0 23.433752 1 CGCAGAG 9240 0.0 23.340847 8 TACCTGG 960 0.0 23.276558 2 ACGCAGA 9305 0.0 23.22885 7 GAGTACT 4925 0.0 22.616186 12-13 AGTACTT 5030 0.0 22.049648 12-13 >>END_MODULE