FastQCFastQC Report
Thu 26 May 2016
SRR937341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937341_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090821
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76100.6976396677365031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61470.5635205042807206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.38796466147974784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.1332940968316525No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12120.111108972049493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12090.1108339498414497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51400.035.6992531
GTATCAA78350.034.098261
TATCAAC98450.027.1216752
ATCAACG98450.027.1216753
TCAACGC99450.026.8489574
CAACGCA100500.026.615725
AACGCAG102850.025.9992396
TATAGGG2800.025.4524232
GTACATG59200.023.9292741
TACATGG59150.023.695352
GAGTACT63800.023.45341512-13
ACGCAGA116200.022.933647
CGCAGAG116000.022.8503288
AGTACTT65050.022.34551612-13
ACATGGG59550.022.1000823
GTACTTT66900.022.0795814-15
GTATAGG3059.094947E-1221.820421
TAAGGTG3950.021.650675
GCAGAGT121800.021.4112139
AGAGTAC100200.021.2860410-11
Produced by FastQC (version 0.11.5)