FastQCFastQC Report
Thu 26 May 2016
SRR937340_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937340_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198927
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68460.5710105786257211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.4400601537875116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36340.30310435914780465No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19320.1611440896735164No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.10284195785064479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.035.6146931
GTATCAA75300.034.139941
ATCAACG92000.027.7785433
TATCAAC92800.027.6426072
TCAACGC93000.027.4856014
CAACGCA93800.027.1971155
TACATGG63500.026.632352
AACGCAG96050.026.6105886
GTACATG65850.026.1945721
ACATGGG65800.025.0506293
CGCAGAG105850.023.8755848
ACGCAGA106650.023.741037
GTATAGG2903.6379788E-1222.9398461
CATGGGG25050.022.5707054
GAGTACT56200.022.27060712-13
GCAGAGT111350.022.1407439
CATGGGA38650.022.0043964
AGTACTT57650.021.58687412-13
GTACTTT59150.020.95782714-15
TACCTGG10300.020.7543182
Produced by FastQC (version 0.11.5)