##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937340_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1198927 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58952796959281 33.0 31.0 34.0 30.0 34.0 2 31.677878636480788 33.0 31.0 34.0 30.0 34.0 3 31.709114900239964 33.0 31.0 34.0 30.0 34.0 4 35.33861444441572 37.0 35.0 37.0 33.0 37.0 5 35.35079199984653 37.0 35.0 37.0 33.0 37.0 6 35.367410192613896 37.0 35.0 37.0 33.0 37.0 7 35.25818169079518 37.0 35.0 37.0 33.0 37.0 8 35.25966301534622 37.0 35.0 37.0 33.0 37.0 9 36.85417293963686 39.0 37.0 39.0 33.0 39.0 10-11 36.73558565283791 39.0 37.0 39.0 32.5 39.0 12-13 36.571323358302884 39.0 37.0 39.0 32.0 39.0 14-15 37.69311392603553 40.0 37.0 41.0 32.0 41.0 16-17 37.62989781696467 40.0 37.0 41.0 32.0 41.0 18-19 37.616479568814455 40.0 37.0 41.0 32.0 41.0 20-21 37.508571831312494 40.0 37.0 41.0 32.0 41.0 22-23 37.351223635801006 40.0 37.0 41.0 32.0 41.0 24-25 37.282125183601664 39.5 37.0 41.0 31.5 41.0 26-27 37.288050064766246 40.0 37.0 41.0 31.5 41.0 28-29 37.19517785486522 40.0 37.0 41.0 31.0 41.0 30-31 36.97542177296866 39.0 36.0 41.0 31.0 41.0 32-33 36.71820719693525 39.0 36.0 41.0 30.0 41.0 34-35 36.730802626014764 39.0 36.0 41.0 30.0 41.0 36-37 36.5924397398674 39.0 35.5 41.0 30.0 41.0 38-39 36.462433909654216 39.0 35.0 41.0 30.0 41.0 40-41 36.23745982866346 39.0 35.0 41.0 30.0 41.0 42-43 35.94228088949535 39.0 35.0 41.0 29.0 41.0 44-45 35.66910662617491 39.0 35.0 40.0 27.5 41.0 46-47 35.28406316648136 39.0 35.0 40.0 26.0 41.0 48-49 34.91195627423521 38.5 34.5 40.0 24.0 41.0 50-51 33.92752769768302 37.5 33.5 39.5 23.5 40.5 52-53 34.047437416956996 38.0 33.5 39.5 23.0 40.5 54-55 34.41760007072991 38.0 34.0 40.0 23.0 41.0 56-57 34.184749363389095 38.0 34.0 40.0 22.5 41.0 58-59 33.90227470062815 38.0 33.5 40.0 21.5 41.0 60-61 33.57614642092471 37.5 33.0 40.0 20.5 41.0 62-63 33.25059866030209 37.0 33.0 40.0 18.5 41.0 64-65 32.81900899721167 36.5 32.0 39.5 17.5 41.0 66-67 32.45313684653027 36.0 32.0 39.0 14.0 41.0 68-69 31.952627224176283 35.5 31.0 39.0 9.5 40.0 70-71 31.474968033916994 35.0 31.0 38.0 7.5 40.0 72-73 31.003988566443162 35.0 30.5 37.5 6.5 40.0 74-75 30.50639905515515 35.0 30.0 37.0 2.0 39.0 76-77 30.014253578408027 34.0 29.5 36.5 2.0 39.0 78-79 29.502327080798082 34.0 29.0 36.0 2.0 38.5 80-81 29.005298487731114 34.0 29.0 35.5 2.0 37.0 82-83 28.506718090425856 34.0 27.5 35.0 2.0 37.0 84-85 27.972526684276858 34.0 26.5 35.0 2.0 36.0 86-87 27.497160794610515 33.0 26.0 35.0 2.0 36.0 88-89 27.006127145355805 33.0 25.5 35.0 2.0 35.5 90-91 26.467910056241955 32.5 25.0 35.0 2.0 35.0 92-93 25.937323957171706 32.0 23.5 35.0 2.0 35.0 94-95 25.354832279196316 32.0 20.5 34.0 2.0 35.0 96-97 24.58173391707752 32.0 13.0 34.0 2.0 35.0 98-99 23.609861150845717 31.0 2.0 34.0 2.0 35.0 100-101 21.068575484579128 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06872319364541823 1101 2 -0.001567662059436259 1101 3 -0.07056108783434212 1101 4 -0.13808498214643805 1101 5 -0.19432682087434472 1101 6 -0.2078621981015445 1101 7 -0.2265091011673519 1101 8 -0.24178697590053844 1101 9 -0.2660602236758791 1101 10-11 -0.41831112540639737 1101 12-13 -0.2526182704793385 1101 14-15 -0.5028497596597106 1101 16-17 -0.40777006596410814 1101 18-19 -0.11906267048152586 1101 20-21 -0.08560394804452187 1101 22-23 -0.2996103483998951 1101 24-25 -0.2291343814893949 1101 26-27 -0.2669534941500018 1101 28-29 -0.3598034701999282 1101 30-31 -0.21746078465938012 1101 32-33 -0.3193380769158054 1101 34-35 -0.10010392848777627 1101 36-37 -0.22484658902717314 1101 38-39 -0.2505935043086467 1101 40-41 -0.16300150871828833 1101 42-43 -0.11881480281145684 1101 44-45 -0.11951637690390271 1101 46-47 -0.1601843108713723 1101 48-49 -0.02720607871233227 1101 50-51 0.022364121379943924 1101 52-53 -0.12773599166678906 1101 54-55 0.047366256208199786 1101 56-57 0.04534651362919817 1101 58-59 -0.017371707673341064 1101 60-61 -0.004981914042069491 1101 62-63 -0.031383198964007875 1101 64-65 -0.03216346308585116 1101 66-67 -0.03023875323861347 1101 68-69 0.18436907338235642 1101 70-71 -0.21401662790284703 1101 72-73 -0.029124385190709035 1101 74-75 -0.12956495592416672 1101 76-77 -0.028432089279753825 1101 78-79 -0.012102498860265598 1101 80-81 0.004847731388444743 1101 82-83 0.10743419998102866 1101 84-85 0.08769465471124605 1101 86-87 0.14950843789156565 1101 88-89 0.2130118961468277 1101 90-91 0.15603903816318976 1101 92-93 0.293341312675512 1101 94-95 0.24218197480710302 1101 96-97 0.5308490174592428 1101 98-99 0.6570250567477913 1101 100-101 0.7978572688350241 1102 1 2.696425373081013E-4 1102 2 0.01129407147097794 1102 3 -0.133904406037761 1102 4 -0.018078616246732793 1102 5 -0.04155089618380714 1102 6 -0.04484502325295381 1102 7 -0.025219352445979837 1102 8 0.06634960316960559 1102 9 -0.053956293822864154 1102 10-11 -9.921720300738457E-4 1102 12-13 0.08484722939639511 1102 14-15 0.17390445992894854 1102 16-17 0.06089335670982621 1102 18-19 0.08029964949650292 1102 20-21 -0.009841457146457344 1102 22-23 -0.131216604422427 1102 24-25 -0.24481302563996366 1102 26-27 -0.2048861900213268 1102 28-29 0.07577798497498378 1102 30-31 0.10402935909658595 1102 32-33 -0.035033943877223805 1102 34-35 0.14555439710618856 1102 36-37 0.2037707462769731 1102 38-39 0.1697850051115708 1102 40-41 0.09891951052329517 1102 42-43 0.13813209424845496 1102 44-45 0.1886877853480584 1102 46-47 0.18775004187795474 1102 48-49 0.0793309590514113 1102 50-51 0.0908535718632777 1102 52-53 0.02412494394605602 1102 54-55 0.2329056354021688 1102 56-57 0.3378975389314647 1102 58-59 0.052422098219047086 1102 60-61 0.09763529917633207 1102 62-63 0.23479185455750695 1102 64-65 0.23280424959406076 1102 66-67 -0.09055281882481836 1102 68-69 0.1619024616542717 1102 70-71 0.03863176078329289 1102 72-73 0.0746707374836646 1102 74-75 0.12447695436896922 1102 76-77 0.06358241276300092 1102 78-79 -0.06875813420757737 1102 80-81 0.0017149238958680257 1102 82-83 0.20179841664835152 1102 84-85 0.03913984475079246 1102 86-87 0.13440948303277267 1102 88-89 -0.018532226950487285 1102 90-91 0.11748697513206352 1102 92-93 0.13716333781645673 1102 94-95 0.1427461853701395 1102 96-97 0.20424369888084115 1102 98-99 0.3062245230586669 1102 100-101 0.6908711299276078 1103 1 -0.07135465180300571 1103 2 -0.11688123338710454 1103 3 -0.12781903267666195 1103 4 -0.05030123141170151 1103 5 -0.24620464813214937 1103 6 -0.10408114255037049 1103 7 -0.25314186413602613 1103 8 -0.2230160562538117 1103 9 -0.09554797391507464 1103 10-11 -0.12792381308997136 1103 12-13 -0.15427415061854077 1103 14-15 -0.4006963757292965 1103 16-17 -0.307916812648493 1103 18-19 -0.3678731667241735 1103 20-21 -0.37658016331696587 1103 22-23 -0.3369168938902547 1103 24-25 -0.4694865557066308 1103 26-27 -0.4869725850335769 1103 28-29 -0.5991182126490244 1103 30-31 -0.5534070824138837 1103 32-33 -0.337459710443035 1103 34-35 -0.2772585490475521 1103 36-37 -0.29848998433458007 1103 38-39 -0.3758624990470665 1103 40-41 -0.3449132530430461 1103 42-43 -0.30307057252299785 1103 44-45 -0.4253992121764085 1103 46-47 -0.3009751208658358 1103 48-49 -0.18502716896934857 1103 50-51 -0.299022258228284 1103 52-53 -0.22837155231443518 1103 54-55 -0.0014572079315584574 1103 56-57 -0.017482502196543237 1103 58-59 -0.19655952515842756 1103 60-61 -0.028610001883301095 1103 62-63 -0.017398146359354882 1103 64-65 -0.149339777021261 1103 66-67 -0.04505201329218522 1103 68-69 -0.04824135539158547 1103 70-71 -0.0616819385107199 1103 72-73 -0.24692801557801403 1103 74-75 -0.12876482330402084 1103 76-77 -0.15139983932286682 1103 78-79 -0.14072821816634473 1103 80-81 -0.11506766088152887 1103 82-83 -0.12222413826539835 1103 84-85 -0.11530106320891775 1103 86-87 0.19312691790251435 1103 88-89 0.3048197056729265 1103 90-91 0.03862074594165321 1103 92-93 0.0948885922468854 1103 94-95 -0.2657204210453088 1103 96-97 0.07133670842935302 1103 98-99 0.005656720787456493 1103 100-101 0.5738259284134699 1104 1 0.07793776835214317 1104 2 0.07153875939458487 1104 3 0.1674597679893992 1104 4 0.0032016351757206962 1104 5 0.06489266759133017 1104 6 0.08493714060334412 1104 7 -0.004567657157558358 1104 8 0.0906103158210243 1104 9 0.022958082671898694 1104 10-11 0.04118454894453549 1104 12-13 0.12458872705225588 1104 14-15 0.10964884134509845 1104 16-17 0.21303416019106436 1104 18-19 0.17669020043285855 1104 20-21 0.28539040926809633 1104 22-23 0.0196493870644332 1104 24-25 0.12426986951721375 1104 26-27 0.15148398464474866 1104 28-29 0.14046487249969886 1104 30-31 0.048779015894581335 1104 32-33 0.037637247012163755 1104 34-35 0.1880423742433237 1104 36-37 0.12279622524883393 1104 38-39 0.030752359909065774 1104 40-41 0.19675021479972798 1104 42-43 0.24477942072638825 1104 44-45 0.3010909764791947 1104 46-47 0.22470922889930733 1104 48-49 0.3687227791876495 1104 50-51 0.013358558005677423 1104 52-53 0.034176661682913334 1104 54-55 0.053750300877339896 1104 56-57 0.06427833343332878 1104 58-59 0.09315089524204012 1104 60-61 -0.06143214109788886 1104 62-63 0.15470188814381913 1104 64-65 0.10330355455602813 1104 66-67 0.16547847083767664 1104 68-69 -0.009234896855844 1104 70-71 0.1356612286690826 1104 72-73 0.10257327674683836 1104 74-75 0.05918439084421934 1104 76-77 0.12686540980153538 1104 78-79 0.19489569368200677 1104 80-81 0.16541580715537663 1104 82-83 0.16158809209580838 1104 84-85 0.19841752323742057 1104 86-87 0.28211974283021846 1104 88-89 0.047706624380442264 1104 90-91 0.3573692071970598 1104 92-93 0.29788419154764156 1104 94-95 0.36782540243785533 1104 96-97 0.28922051655639436 1104 98-99 0.44928925225285354 1104 100-101 0.998525614002169 1105 1 0.0163599333139679 1105 2 0.003937700802644883 1105 3 -0.16187224404184164 1105 4 -0.0734031789312013 1105 5 -0.02389498705870352 1105 6 0.009444939177512879 1105 7 -0.0075258295391833485 1105 8 -0.14828614054336242 1105 9 0.00464066786860684 1105 10-11 -0.08601060278310513 1105 12-13 -0.06342895311453134 1105 14-15 -0.05555133502726761 1105 16-17 -0.08293355794228319 1105 18-19 -0.02777116176639538 1105 20-21 -0.08933132576373026 1105 22-23 -0.03815258576599234 1105 24-25 0.067494424986414 1105 26-27 0.055284576547542486 1105 28-29 -0.005569119427597968 1105 30-31 0.18942636149944292 1105 32-33 0.05648525463946186 1105 34-35 0.18919523416771256 1105 36-37 0.20495314656726293 1105 38-39 -0.04599264876198106 1105 40-41 0.035684548291960994 1105 42-43 0.09740743155557396 1105 44-45 0.04197810621573694 1105 46-47 0.09749864906174821 1105 48-49 0.1814331269327738 1105 50-51 -0.050262495416717456 1105 52-53 0.0024835227646278213 1105 54-55 -0.016260002416935038 1105 56-57 -0.0014411665349172154 1105 58-59 0.04005158919395768 1105 60-61 0.0765456942980407 1105 62-63 0.043628216906562045 1105 64-65 0.02637251418267539 1105 66-67 0.16682966023315515 1105 68-69 -0.021383080050561887 1105 70-71 -0.0755634591688299 1105 72-73 0.21388686627325626 1105 74-75 0.30488039536622225 1105 76-77 0.10229916328627553 1105 78-79 0.12494576125265766 1105 80-81 0.17739562271567877 1105 82-83 0.306632672625625 1105 84-85 0.43621836020120597 1105 86-87 0.3169190745471049 1105 88-89 0.06426389307729963 1105 90-91 0.13740192373393612 1105 92-93 0.08418698139710656 1105 94-95 0.011221411163457873 1105 96-97 0.1613234976249842 1105 98-99 0.3208149089760681 1105 100-101 0.24767170165709018 1106 1 -0.28255015204719314 1106 2 -0.13753879500894683 1106 3 -0.16887172629920144 1106 4 0.08084762091480258 1106 5 -0.05705042933040261 1106 6 -0.35506911288880616 1106 7 -0.4725582880202097 1106 8 -0.3177306956165751 1106 9 -0.4897172648779531 1106 10-11 -0.7248950665212064 1106 12-13 -0.466133162760471 1106 14-15 -0.45275286966955974 1106 16-17 -0.3790226096767242 1106 18-19 -0.608846008766804 1106 20-21 -0.4096547157832404 1106 22-23 -0.4533921413038513 1106 24-25 -0.4232210555185887 1106 26-27 -0.6810533235414056 1106 28-29 -0.3122005397682486 1106 30-31 -0.44618072493702243 1106 32-33 -0.337928435007008 1106 34-35 -0.19314775041949162 1106 36-37 -0.5512946132001488 1106 38-39 -0.470294359967788 1106 40-41 -0.28672474717978247 1106 42-43 -0.4638753754060758 1106 44-45 -0.26320297095446676 1106 46-47 -0.41446751181018726 1106 48-49 -0.012965959716105147 1106 50-51 -0.22875427127398495 1106 52-53 -0.1896238889204227 1106 54-55 -0.35364898644656506 1106 56-57 -0.24786343212362283 1106 58-59 -0.23947799515163837 1106 60-61 -0.4412069043717537 1106 62-63 -0.43021748251064906 1106 64-65 -0.399221894196792 1106 66-67 -0.32241556392285986 1106 68-69 -0.3730415159367624 1106 70-71 -0.2137725342680632 1106 72-73 -0.530417964663858 1106 74-75 -0.11676954423046837 1106 76-77 -0.10611880105586025 1106 78-79 -0.10409893719544172 1106 80-81 -0.34247275117416365 1106 82-83 -0.06928001472419965 1106 84-85 -0.28618695263482863 1106 86-87 -0.06299948055265858 1106 88-89 0.04913817454344027 1106 90-91 0.23095569595372467 1106 92-93 0.35130899607975863 1106 94-95 0.3360468389649114 1106 96-97 0.31194678011333465 1106 98-99 0.44887158188219445 1106 100-101 0.9173884035037041 1107 1 0.029414857420281493 1107 2 -0.08181762385892455 1107 3 0.01606090597158527 1107 4 0.06220217390918492 1107 5 -0.11307479438368517 1107 6 -0.14985378653480552 1107 7 0.02375643898790969 1107 8 -0.1339991024206384 1107 9 -0.11584788472365659 1107 10-11 -0.024261880285820325 1107 12-13 0.1804532651067703 1107 14-15 0.14986750809048743 1107 16-17 -0.0641312900203701 1107 18-19 -0.06701796470002108 1107 20-21 -0.011624693718552237 1107 22-23 0.15331939806050343 1107 24-25 -0.017571086033584038 1107 26-27 -0.008641621430392377 1107 28-29 -0.08257908224668853 1107 30-31 0.057826324810250185 1107 32-33 0.05790294223662329 1107 34-35 -0.05887881938338779 1107 36-37 -0.12650821819522662 1107 38-39 -0.17041016047793534 1107 40-41 -0.23844356319781213 1107 42-43 -0.08256111785947695 1107 44-45 6.852659045222254E-4 1107 46-47 0.17285428841828576 1107 48-49 0.12422634737433214 1107 50-51 0.20297428607074153 1107 52-53 0.03073254974443529 1107 54-55 -0.19388536032573 1107 56-57 -0.030836258125532368 1107 58-59 0.06001694248822531 1107 60-61 0.15445468428605835 1107 62-63 0.3122574404194225 1107 64-65 0.39602539466289244 1107 66-67 0.1084640504927421 1107 68-69 0.1480236140258313 1107 70-71 0.13043763583349843 1107 72-73 0.4797840211584017 1107 74-75 0.27956650415536544 1107 76-77 0.11104334313142417 1107 78-79 0.29113387660046897 1107 80-81 0.40292945998963603 1107 82-83 0.41556909251646346 1107 84-85 0.2545006911377641 1107 86-87 0.4661599485821206 1107 88-89 0.4868357396029843 1107 90-91 0.5108095369314576 1107 92-93 0.5076885870222831 1107 94-95 0.5038431474324341 1107 96-97 0.4974603710245624 1107 98-99 0.7306658638407875 1107 100-101 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1.5280008258631597 2310 84-85 1.4957966928412993 2310 86-87 1.359820544318275 2310 88-89 1.3132521160841826 2310 90-91 1.545312380331513 2310 92-93 1.9815030019332234 2310 94-95 2.0338068846872375 2310 96-97 2.0711038061524505 2310 98-99 2.1114935813546296 2310 100-101 1.7947326147307976 2311 1 0.5285196764776892 2311 2 0.5688007140884572 2311 3 0.5080788759001358 2311 4 0.3812968807374233 2311 5 0.4581853385672616 2311 6 0.44139457644449465 2311 7 0.5523276307389864 2311 8 0.4678235854430284 2311 9 0.4126424920812113 2311 10-11 0.7030467333441379 2311 12-13 0.6761255211285189 2311 14-15 0.6302978580399312 2311 16-17 0.718538670028039 2311 18-19 0.6304305623957802 2311 20-21 0.8287875453942277 2311 22-23 0.7526772300648759 2311 24-25 0.8436074859870359 2311 26-27 0.7548731241890252 2311 28-29 1.0973999473108549 2311 30-31 0.9208356661891628 2311 32-33 0.9116185648018131 2311 34-35 0.966004931424763 2311 36-37 1.0129618015319153 2311 38-39 1.2113202740163658 2311 40-41 1.3547640441824598 2311 42-43 1.5243736333962659 2311 44-45 1.3590083764925254 2311 46-47 1.229897207371252 2311 48-49 1.3860046077393733 2311 50-51 1.2425321661496511 2311 52-53 1.246200737666122 2311 54-55 1.2571594318762536 2311 56-57 1.4630725743264321 2311 58-59 1.1922467719065253 2311 60-61 1.3141729923940701 2311 62-63 1.235226291438103 2311 64-65 1.3182244315145013 2311 66-67 1.3060027173362343 2311 68-69 1.32814147951661 2311 70-71 1.5038244451765372 2311 72-73 1.396002751990757 2311 74-75 1.4777672319043162 2311 76-77 1.4795460286742532 2311 78-79 1.602527333490876 2311 80-81 1.6242784893814068 2311 82-83 1.6135253032486858 2311 84-85 1.726577055045393 2311 86-87 1.664280897473919 2311 88-89 1.7129673560676828 2311 90-91 1.7098028728066588 2311 92-93 1.7832737701199406 2311 94-95 1.8707751219695083 2311 96-97 1.7547440770736635 2311 98-99 2.0459871343346308 2311 100-101 2.207867503155434 2312 1 0.6523627766559592 2312 2 0.6638074632640318 2312 3 0.5768378819202873 2312 4 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2315 10-11 0.2983715088314014 2315 12-13 0.6929754145911673 2315 14-15 0.8833599895710549 2315 16-17 0.7430389902936625 2315 18-19 0.49224574952241085 2315 20-21 0.5371856943896418 2315 22-23 0.6904182462873081 2315 24-25 0.822045112622682 2315 26-27 0.8160144462978138 2315 28-29 0.7915103278387079 2315 30-31 1.0026602652035308 2315 32-33 0.7127662922230869 2315 34-35 0.9032132609454138 2315 36-37 1.0128735650843765 2315 38-39 0.9636895860335173 2315 40-41 1.000141761538245 2315 42-43 1.0411222183450803 2315 44-45 0.8062527849161611 2315 46-47 1.0466268378239363 2315 48-49 1.5181795381306173 2315 50-51 1.3377785092391008 2315 52-53 1.4887300517748017 2315 54-55 1.401200530452705 2315 56-57 1.0959926124968717 2315 58-59 1.2016259794778605 2315 60-61 1.067205711776026 2315 62-63 0.9248137370256231 2315 64-65 1.1423691915767265 2315 66-67 1.161010625936349 2315 68-69 1.2492221472377594 2315 70-71 1.2832921506367434 2315 72-73 1.1169370125693234 2315 74-75 1.0523727823036673 2315 76-77 0.9831931351723746 2315 78-79 0.9646889303744928 2315 80-81 0.916805189076829 2315 82-83 1.0639690998996265 2315 84-85 1.386817711786005 2315 86-87 1.5748646959549468 2315 88-89 1.727245320485519 2315 90-91 2.253983188889226 2315 92-93 1.991590218700967 2315 94-95 2.3542618342141104 2315 96-97 2.6112748813979003 2315 98-99 2.8471936860542613 2315 100-101 2.4467693188654422 2316 1 -0.17125524038173978 2316 2 -0.07518629495380935 2316 3 -0.15130958333177702 2316 4 -0.01351374567907726 2316 5 -0.019941097408654684 2316 6 -0.015278866081423814 2316 7 0.104439440204942 2316 8 -0.09496993777013074 2316 9 -0.43778625625402867 2316 10-11 -0.29158709766436886 2316 12-13 -0.06332321988568168 2316 14-15 0.01631349703460927 2316 16-17 -0.070502351179627 2316 18-19 0.17005570940077774 2316 20-21 -0.012347761505473898 2316 22-23 -0.04364005149155048 2316 24-25 0.28732891250012216 2316 26-27 0.4639239587100832 2316 28-29 0.22266183037665854 2316 30-31 0.03427484311697526 2316 32-33 0.18958650797805632 2316 34-35 0.3813631563054045 2316 36-37 -0.017463364165102746 2316 38-39 0.34264538285847124 2316 40-41 0.25975014198848356 2316 42-43 0.1240566477969054 2316 44-45 -0.1334057764861285 2316 46-47 -0.198210536579154 2316 48-49 0.07169625162512716 2316 50-51 -0.5459149938307206 2316 52-53 -0.47984036921332773 2316 54-55 -0.4751999690443327 2316 56-57 -0.4697860594422636 2316 58-59 -0.4569701101078536 2316 60-61 -0.7727702383667676 2316 62-63 -0.48804021790402174 2316 64-65 -0.27451138006987463 2316 66-67 -0.46744798634064466 2316 68-69 -0.3097433620295291 2316 70-71 -0.09151851454993576 2316 72-73 -0.2025821059109063 2316 74-75 0.06090585663853787 2316 76-77 0.1323513901702391 2316 78-79 -0.09336314681629787 2316 80-81 -0.10659139075598034 2316 82-83 -0.24789279842035228 2316 84-85 -0.005443552912733907 2316 86-87 0.11393704208789401 2316 88-89 0.32951213515130817 2316 90-91 0.14861764262215615 2316 92-93 -0.2599418034526515 2316 94-95 0.5193299947469079 2316 96-97 0.5912978906362873 2316 98-99 0.646513236969426 2316 100-101 0.7740294851332052 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5413.0 3 1410.0 4 1011.0 5 1128.0 6 1318.0 7 1487.0 8 1962.0 9 2474.0 10 3019.0 11 3997.0 12 5032.0 13 6370.0 14 7329.0 15 9129.0 16 11491.0 17 14720.0 18 12879.0 19 8936.0 20 8991.0 21 9673.0 22 10258.0 23 11320.0 24 12583.0 25 14289.0 26 16818.0 27 19008.0 28 22894.0 29 26880.0 30 32657.0 31 40073.0 32 49670.0 33 63652.0 34 85183.0 35 117676.0 36 165694.0 37 216019.0 38 158587.0 39 17894.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78880117237883 19.154374923673252 14.956362012115251 22.100461891832662 2 14.998566030731258 20.23811415064286 40.689024323965015 24.07429549466087 3 19.199318469298095 25.19071907675664 29.917375270768837 25.692587183176425 4 12.250231802946175 16.387903941193535 37.84437055362653 33.51749370223375 5 13.256875211114046 37.91725332682294 34.05440973348629 14.771461728576732 6 31.16287317068683 36.525206193715455 17.650901347358104 14.661019288239618 7 28.119440585409755 33.489432564379435 20.417913244067655 17.973213606143158 8 26.68320292768759 34.422825392471374 19.713386343033193 19.180585336807844 9 26.68064872748194 16.514385968433324 20.226907439091903 36.578057864992836 10-11 24.647508926484406 27.09637007057139 27.081818406099323 21.174302596844882 12-13 25.88344752462413 24.33221616129093 27.980706306411783 21.803630007673153 14-15 22.835350506481323 25.429927970445643 26.769799765531843 24.964921757541195 16-17 21.48722211861012 28.926678035191134 27.240258626962138 22.345841219236615 18-19 21.4228612427228 27.11112021661435 30.289883915983335 21.17613462467952 20-21 22.50191268139118 25.96376148131425 30.453232327032982 21.08109351026159 22-23 21.9949162391876 26.00590318278545 30.333830839531256 21.665349738495692 24-25 21.690295345596862 26.10825144152848 30.5941818811168 21.607271331757854 26-27 21.465408213393538 26.30936627888746 30.484044610287125 21.741180897431878 28-29 21.360276422580643 26.634484829249345 30.48137689422688 21.52386185394313 30-31 21.58589203081393 26.33489532848315 30.397058442259006 21.682154198443914 32-33 21.383588887532177 26.305341966476597 30.39122624199006 21.919842904001165 34-35 21.725064319385478 26.521709674505434 30.239395247223978 21.513830758885106 36-37 21.76676131802858 26.198150786643122 30.354073018676086 21.68101487665222 38-39 21.552059673313526 26.495877643285393 30.23745828206778 21.7146044013333 40-41 21.721770640161466 26.507346731970948 30.12222814917871 21.648654478688876 42-43 21.498992861819303 26.520845544552802 29.933576540411366 22.046585053216532 44-45 21.617403718651087 26.708709846442453 29.73921436041802 21.934672074488436 46-47 21.931490751544196 26.922896776109166 29.243688341885488 21.901924130461147 48-49 21.877996748821584 27.034589878522024 29.051248926764007 22.036164445892386 50-51 21.968645078617325 27.234307901547254 28.868342118778322 21.9287049010571 52-53 22.01075088893537 27.370968416649237 28.5595482116712 22.058732482744194 54-55 21.84658094082625 27.352515211548106 28.60837493533341 22.192528912292236 56-57 21.91073568106246 27.614648906666357 28.400880920987177 22.073734491284018 58-59 21.984349765438747 27.26486195493348 28.561552685166298 22.18923559446148 60-61 21.920030226165444 27.24337956281563 28.955889107755528 21.880701103263398 62-63 21.8882227569669 27.279920448717387 28.944177420426776 21.88767937388894 64-65 21.81337842740284 27.284600865659016 28.849156659539215 22.052864047398927 66-67 21.695709943119958 27.372763866940236 28.66768453622639 22.26384165371342 68-69 21.775155546378862 27.48475047794514 28.691086521654448 22.049007454021552 70-71 21.87762799539228 27.225823278333866 28.589538676568687 22.307010049705163 72-73 21.75833233713394 27.419322478521803 28.458017092273703 22.364328092070558 74-75 21.692590007147903 27.76630119006316 28.21549046737254 22.325618335416397 76-77 21.82006853289763 27.52842576689784 28.34561953037071 22.30588616983382 78-79 21.740406394148646 27.491386092835384 28.353612908026633 22.414594604989336 80-81 21.738407073283188 27.647454469982396 28.168436506281004 22.445701950453408 82-83 21.849579690378253 27.59066929777299 28.283152963777425 22.27659804807133 84-85 21.79043364350663 27.51081956139918 28.165928643142983 22.5328181519512 86-87 21.675985967540743 27.610268175144892 28.220175171643465 22.493570685670903 88-89 21.829551378170574 27.38541580884151 28.100533694396727 22.684499118591184 90-91 21.686843196671415 27.263358485012677 28.350449519375488 22.69934879894042 92-93 21.74752890365504 27.149872630794842 28.34192633068841 22.76067213486171 94-95 21.65528363254989 27.111532276638872 28.35765369186094 22.8755303989503 96-97 21.50488726535127 27.092059348679555 28.3764897892295 23.026563596739678 98-99 21.46576982767848 27.15786551428877 28.236965508937175 23.13939914909558 100-101 21.628354195897874 26.67340421129977 28.686266568529227 23.01197502427313 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1954.0 1 1140.0 2 401.5 3 452.0 4 361.5 5 351.0 6 582.5 7 1226.5 8 2022.0 9 2395.5 10 2117.5 11 1781.5 12 1814.0 13 1891.0 14 1995.0 15 2144.5 16 2227.0 17 2296.0 18 2465.0 19 2625.0 20 2825.0 21 3233.5 22 3723.5 23 4399.0 24 5207.5 25 6180.5 26 7840.0 27 9651.5 28 11102.5 29 12742.5 30 14619.0 31 16987.0 32 19649.0 33 21586.0 34 23585.5 35 25933.5 36 28971.0 37 31670.5 38 33063.0 39 34084.5 40 35413.5 41 37176.0 42 38733.0 43 40177.5 44 42033.5 45 43394.0 46 44247.0 47 45993.0 48 48234.5 49 47794.5 50 44606.0 51 41570.5 52 39664.5 53 39064.0 54 38251.5 55 35403.5 56 31468.5 57 28083.0 58 25515.0 59 22820.5 60 19059.5 61 15751.0 62 12653.0 63 9247.5 64 6665.0 65 4955.5 66 3550.0 67 2500.5 68 1854.5 69 1412.0 70 1043.0 71 698.0 72 458.5 73 334.5 74 238.0 75 162.5 76 122.0 77 79.0 78 53.5 79 36.5 80 21.0 81 14.0 82 13.5 83 10.5 84 2.5 85 2.5 86 4.0 87 3.5 88 4.0 89 4.5 90 2.5 91 0.5 92 0.5 93 1.0 94 0.5 95 1.5 96 1.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28433757851812497 2 0.2457197143779396 3 0.23320852729148644 4 0.239213897092984 5 0.2411322791129068 6 0.27758153749144027 7 0.27691427418016273 8 0.26448649500761934 9 0.25931520434521865 10-11 0.26586272558712915 12-13 0.26686362055404544 14-15 0.2526425712324437 16-17 0.26924074610047155 18-19 0.2559788877888312 20-21 0.24705424100049464 22-23 0.24705424100049464 24-25 0.2408403514142229 26-27 0.2514331564807532 28-29 0.2686985946600585 30-31 0.2697411935839297 32-33 0.26899052235874243 34-35 0.2778317612331693 36-37 0.24680401725876555 38-39 0.2459282341627138 40-41 0.24063183162944868 42-43 0.247221056828314 44-45 0.2712008320773492 46-47 0.27708109000798214 48-49 0.2802922946935051 50-51 0.2826694202399312 52-53 0.3066908994459212 54-55 0.2812097817465117 56-57 0.2423833978215521 58-59 0.2211143797745818 60-61 0.21790317508905882 62-63 0.226661006049576 64-65 0.21794487904601367 66-67 0.21998837293680096 68-69 0.22186505099976894 70-71 0.2229493538805949 72-73 0.2263690783508921 74-75 0.23120673735765396 76-77 0.25064078129861117 78-79 0.24154931868245522 80-81 0.2352520211822738 82-83 0.22732826936085349 84-85 0.2263690783508921 86-87 0.23712869924524177 88-89 0.23608610032137067 90-91 0.24880580719259804 92-93 0.23337534311930583 94-95 0.23149866505633784 96-97 0.2427587334341457 98-99 0.2521421237489856 100-101 0.22065563624807852 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1198927.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.66818889993546 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61101513077217 50.51746323630787 2 11.404550166757216 14.294050083382636 3 3.4422130825999186 6.4715177908260255 4 1.4766903424261915 3.7016603730349975 5 0.8082248023173548 2.5324992292618496 6 0.48266580284209315 1.8148675024828378 7 0.33458406650882155 1.4677444238018378 8 0.2350942131929283 1.1786342849324905 9 0.17988846154296156 1.0145955680003782 >10 0.9502282202810549 10.55471471040222 >50 0.05249955985072549 2.248916538954913 >100 0.021149039886482188 2.362659751415756 >500 3.9903700733449486E-4 0.17954973265558793 >1k 5.320493431126599E-4 0.6506802289273536 >5k 2.660246715563299E-4 1.010446545613295 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6846 0.5710105786257211 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5276 0.4400601537875116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3634 0.30310435914780465 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1932 0.1611440896735164 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1233 0.10284195785064479 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.170395695484379E-5 0.0 14-15 0.0 0.0 0.0 8.340791390968757E-5 0.0 16-17 0.0 0.0 0.0 8.340791390968757E-5 0.0 18-19 0.0 0.0 0.0 8.340791390968757E-5 0.0 20-21 0.0 2.502237417290627E-4 0.0 8.340791390968757E-5 0.0 22-23 0.0 2.502237417290627E-4 0.0 8.340791390968757E-5 0.0 24-25 0.0 3.336316556387503E-4 0.0 8.340791390968757E-5 0.0 26-27 0.0 4.170395695484379E-4 0.0 1.6681582781937515E-4 0.0 28-29 0.0 4.170395695484379E-4 0.0 0.001000894966916251 0.0 30-31 0.0 4.170395695484379E-4 0.0 0.002543941374245471 0.0 32-33 0.0 4.170395695484379E-4 0.0 0.004253803609394067 0.0 34-35 0.0 4.170395695484379E-4 0.0 0.005588330231949068 0.0 36-37 0.0 4.170395695484379E-4 0.0 0.008090567649239695 0.0 38-39 0.0 5.004474834581254E-4 0.0 0.012260963344724075 0.0 40-41 0.0 5.421514404129692E-4 0.0 0.01805781336144736 0.0 42-43 0.0 6.255593543226569E-4 0.0 0.0218111694873833 0.0 44-45 0.0 6.672633112775006E-4 0.0 0.02443851877553846 0.0 46-47 0.0 6.672633112775006E-4 0.0 0.028483802600158308 0.0 48-49 0.0 6.672633112775006E-4 0.0 0.03448917240165582 0.0 50-51 0.0 6.672633112775006E-4 0.0 0.040994989686611444 0.0 52-53 0.0 6.672633112775006E-4 0.0 0.058218723908961933 0.0 54-55 0.0 6.672633112775006E-4 0.0 0.07106354265105382 0.0 56-57 0.0 6.672633112775006E-4 0.0 0.0987549700690701 0.0 58-59 0.0 6.672633112775006E-4 0.0 0.1484660867592439 0.0 60-61 0.0 6.672633112775006E-4 0.0 0.19242205738964924 0.0 62-63 0.0 6.672633112775006E-4 0.0 0.21861214235729115 0.0 64-65 0.0 6.672633112775006E-4 0.0 0.2597739478717219 0.0 66-67 0.0 6.672633112775006E-4 0.0 0.3499796067650491 0.0 68-69 0.0 7.506712251871882E-4 0.0 0.4772183794342775 0.0 70-71 0.0 8.340791390968758E-4 0.0 0.5960746567555824 0.0 72-73 0.0 8.340791390968758E-4 0.0 0.7271918974216112 0.0 74-75 0.0 9.174870530065634E-4 0.0 0.8888781385355405 0.0 76-77 0.0 9.174870530065634E-4 0.0 1.0012703025288445 0.0 78-79 0.0 9.174870530065634E-4 0.0 1.1622475763745417 0.0 80-81 0.0 9.174870530065634E-4 0.0 1.3785242971423615 0.0 82-83 0.0 9.174870530065634E-4 0.0 1.5848337721979737 0.0 84-85 0.0 9.174870530065634E-4 0.0 1.8297611113937713 0.0 86-87 0.0 9.174870530065634E-4 0.0 2.1324484309720275 0.0 88-89 0.0 9.174870530065634E-4 0.0 2.5260086727548883 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4950 0.0 35.614693 1 GTATCAA 7530 0.0 34.13994 1 ATCAACG 9200 0.0 27.778543 3 TATCAAC 9280 0.0 27.642607 2 TCAACGC 9300 0.0 27.485601 4 CAACGCA 9380 0.0 27.197115 5 TACATGG 6350 0.0 26.63235 2 AACGCAG 9605 0.0 26.610588 6 GTACATG 6585 0.0 26.194572 1 ACATGGG 6580 0.0 25.050629 3 CGCAGAG 10585 0.0 23.875584 8 ACGCAGA 10665 0.0 23.74103 7 GTATAGG 290 3.6379788E-12 22.939846 1 CATGGGG 2505 0.0 22.570705 4 GAGTACT 5620 0.0 22.270607 12-13 GCAGAGT 11135 0.0 22.140743 9 CATGGGA 3865 0.0 22.004396 4 AGTACTT 5765 0.0 21.586874 12-13 GTACTTT 5915 0.0 20.957827 14-15 TACCTGG 1030 0.0 20.754318 2 >>END_MODULE