FastQCFastQC Report
Thu 26 May 2016
SRR937340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937340_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198927
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82630.6891995926357485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65630.5474061389892796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45210.37708717878569753No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.12502846295062167No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13390.11168319672507165No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.10776302477131636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83650.033.7543371
GGTATCA54850.033.7118071
CGTGTCG650.005837728729.2373689
TATCAAC104550.026.8501282
ATCAACG105800.026.5329023
TCAACGC106000.026.482844
CAACGCA106800.026.328945
AACGCAG110450.025.5438026
ACGCAGA122900.022.9628737
TATTCCG1452.3348963E-522.930475
CGCAGAG123350.022.6479978
TACATGG66800.022.3272952
AGTACTT65900.022.31345612-13
GAGTACT65250.022.24448412-13
GTACATG68200.022.2338871
TACCTGG9600.021.7702962
GTACTTT67600.021.35953114-15
GCAGAGT129900.021.3231359
CATGGGA42500.021.123014
ACATGGG69350.020.9583873
Produced by FastQC (version 0.11.5)