##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937340_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1198927 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24893425538002 33.0 31.0 34.0 31.0 34.0 2 32.456276320409835 34.0 31.0 34.0 31.0 34.0 3 32.50376127987776 34.0 31.0 34.0 31.0 34.0 4 36.13578474752841 37.0 35.0 37.0 35.0 37.0 5 36.067045783438026 37.0 35.0 37.0 35.0 37.0 6 36.111556416695926 37.0 35.0 37.0 35.0 37.0 7 36.05674907646587 37.0 35.0 37.0 35.0 37.0 8 36.03144311538568 37.0 35.0 37.0 35.0 37.0 9 37.7480046741795 39.0 37.0 39.0 35.0 39.0 10-11 37.66867790949741 39.0 37.0 39.0 35.0 39.0 12-13 37.56902296803726 39.0 37.0 39.0 35.0 39.0 14-15 38.770296273251 40.0 38.0 41.0 35.0 41.0 16-17 38.747550935127826 40.0 38.0 41.0 35.0 41.0 18-19 38.76012175887273 40.0 38.0 41.0 35.0 41.0 20-21 38.68423056616458 40.0 38.0 41.0 34.0 41.0 22-23 38.539025311799634 40.0 38.0 41.0 34.0 41.0 24-25 38.4872690330604 40.0 38.0 41.0 34.0 41.0 26-27 38.42919835819862 40.0 38.0 41.0 34.0 41.0 28-29 38.30656453645635 40.0 38.0 41.0 34.0 41.0 30-31 38.34316601427777 40.0 38.0 41.0 34.0 41.0 32-33 38.26016054355269 40.0 38.0 41.0 34.0 41.0 34-35 38.135613761304896 40.0 37.0 41.0 34.0 41.0 36-37 37.97212799444837 40.0 37.0 41.0 33.0 41.0 38-39 37.84255713650623 40.0 37.0 41.0 33.0 41.0 40-41 37.660314180930115 40.0 37.0 41.0 33.0 41.0 42-43 37.43538597429201 40.0 37.0 41.0 33.0 41.0 44-45 37.11710012369394 40.0 36.0 41.0 32.0 41.0 46-47 36.72152808302757 40.0 36.0 41.0 31.0 41.0 48-49 36.34943912348291 39.5 36.0 41.0 30.0 41.0 50-51 36.08780851544756 39.0 35.5 41.0 29.5 41.0 52-53 35.84589387010218 39.0 35.0 41.0 29.0 41.0 54-55 35.621075344870874 39.0 35.0 40.0 28.0 41.0 56-57 35.35097549725713 38.5 35.0 40.0 28.0 41.0 58-59 35.08588304375496 38.0 34.5 40.0 27.0 41.0 60-61 34.762481368757236 38.0 34.0 40.0 26.5 41.0 62-63 34.39699539671723 37.5 34.0 40.0 26.0 41.0 64-65 33.99447047234736 37.0 33.5 40.0 25.5 41.0 66-67 33.59120697089982 36.0 33.0 39.0 25.0 41.0 68-69 33.15381128292215 36.0 33.0 39.0 23.0 40.0 70-71 32.7473732762712 35.0 32.0 38.5 22.5 40.0 72-73 32.27845690354792 35.0 32.0 37.5 21.5 40.0 74-75 31.80990627452714 35.0 31.0 37.0 20.0 39.0 76-77 30.55451624661051 34.0 29.5 35.5 19.5 38.5 78-79 31.15033442403082 34.0 31.0 36.0 20.0 38.5 80-81 30.96712185145551 34.0 31.0 36.0 18.0 37.5 82-83 30.633439316989275 34.0 31.0 35.0 17.5 37.0 84-85 30.281734000485436 34.0 31.0 35.0 13.0 36.5 86-87 29.889161308403267 34.0 30.0 35.0 7.5 36.0 88-89 29.43569416653391 34.0 30.0 35.0 4.5 36.0 90-91 28.94165741533888 34.0 29.0 35.0 2.0 35.0 92-93 28.613999434494346 34.0 29.0 35.0 2.0 35.0 94-95 28.197218012439457 34.0 29.0 35.0 2.0 35.0 96-97 27.742194895936116 33.5 28.0 35.0 2.0 35.0 98-99 27.039872319165386 33.0 26.5 35.0 2.0 35.0 100-101 24.64019410689725 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09513031373320757 1101 2 -0.08690361205452035 1101 3 -0.08870710921733149 1101 4 -0.09219026493362037 1101 5 -0.02291877220378069 1101 6 -0.25080405581877585 1101 7 -0.09624099385128915 1101 8 -0.022448450392531072 1101 9 0.0075001586987824 1101 10-11 -0.09565726246031403 1101 12-13 -0.18190428411990922 1101 14-15 -0.2655214199321776 1101 16-17 -0.32321948707622994 1101 18-19 -0.12257355647223278 1101 20-21 -0.10911368726011261 1101 22-23 -0.14342810089376457 1101 24-25 -0.0905268985587071 1101 26-27 -0.26224168356685595 1101 28-29 -0.2827806284520449 1101 30-31 -0.286255454795068 1101 32-33 -0.2013034287372264 1101 34-35 -0.357247601631272 1101 36-37 -0.23601625056898712 1101 38-39 -0.16729159118999348 1101 40-41 -0.1756932197290908 1101 42-43 -0.08716927586068124 1101 44-45 -0.2162968059992636 1101 46-47 -0.21326075621961138 1101 48-49 -0.36869609021406546 1101 50-51 -0.14959551838537521 1101 52-53 -0.16225042195701178 1101 54-55 -0.2556487073293212 1101 56-57 -0.18000182295659073 1101 58-59 -0.22938481549658718 1101 60-61 -0.18913845795035655 1101 62-63 -0.27047951969438344 1101 64-65 -0.35428800835667573 1101 66-67 -0.26063023329274415 1101 68-69 -0.22136200420694507 1101 70-71 -0.2850500704913941 1101 72-73 -0.28138844879867975 1101 74-75 -0.22967678307962558 1101 76-77 -0.12439822344637008 1101 78-79 -0.2832358725543287 1101 80-81 -0.24269642813058212 1101 82-83 -0.20601066207488472 1101 84-85 -0.34764121691856076 1101 86-87 -0.23512089594579066 1101 88-89 -0.05977965510884786 1101 90-91 -0.14885022415368 1101 92-93 -0.01686715003634731 1101 94-95 0.03256777290280155 1101 96-97 0.0019813942913025073 1101 98-99 0.19290008304933082 1101 100-101 0.35622735961346663 1102 1 -0.09355584360715596 1102 2 -0.019665758496373087 1102 3 -0.12189699179700142 1102 4 -0.05134691402865599 1102 5 -0.06649727987992549 1102 6 -0.05203581380994393 1102 7 -0.13347365876515482 1102 8 -0.05353235382270327 1102 9 -0.2425087797220371 1102 10-11 -0.09033301579477637 1102 12-13 -0.08923662332644255 1102 14-15 -0.028692292016835097 1102 16-17 -0.16122543439622916 1102 18-19 -0.20460275240677817 1102 20-21 -0.0029780063922544286 1102 22-23 -0.05142515339499454 1102 24-25 -0.17317567966332348 1102 26-27 -0.1036124013002464 1102 28-29 -0.19015656971136252 1102 30-31 -0.08271387805763197 1102 32-33 -0.07220647085244991 1102 34-35 -0.15090066881766973 1102 36-37 -0.17811446855308333 1102 38-39 -0.31182890800686636 1102 40-41 -0.3336266132332568 1102 42-43 -0.32633220365132587 1102 44-45 -0.2725497763890985 1102 46-47 -0.17371587444657877 1102 48-49 -0.10177740368229848 1102 50-51 -0.150603815856023 1102 52-53 -0.042749403849079215 1102 54-55 -0.10416035125751932 1102 56-57 -0.10763396987806573 1102 58-59 -0.08878454603591024 1102 60-61 -0.13275751553558024 1102 62-63 -0.07544579350655312 1102 64-65 -0.10294811727460029 1102 66-67 -0.06773366172215134 1102 68-69 -0.2185027995955906 1102 70-71 -0.05182530626949955 1102 72-73 -0.06502394435780445 1102 74-75 -0.011754159282496346 1102 76-77 -0.02205744724262715 1102 78-79 -0.0707918466895876 1102 80-81 0.10401946525367833 1102 82-83 0.07833838107806557 1102 84-85 -0.11693185512181259 1102 86-87 -0.04696779169763943 1102 88-89 0.015903262042890276 1102 90-91 0.1166359169338449 1102 92-93 0.23173191600768206 1102 94-95 0.35884215190056423 1102 96-97 0.31680238606359623 1102 98-99 0.5918793589936442 1102 100-101 1.2318237266904255 1103 1 -0.0748923728748423 1103 2 -0.054359983547783486 1103 3 -0.18368068991218678 1103 4 -0.16694150189720602 1103 5 -0.11503094852440654 1103 6 -0.24029337468332557 1103 7 -0.1684298456487241 1103 8 -0.16871697142391895 1103 9 -0.07365080232934673 1103 10-11 -0.09687027331635534 1103 12-13 -0.19204419926262517 1103 14-15 -0.2344312208549013 1103 16-17 -0.3192912392084679 1103 18-19 -0.08327971417345026 1103 20-21 -0.3621525646591124 1103 22-23 -0.2487361151878602 1103 24-25 -0.24124973627327506 1103 26-27 -0.2115360918185374 1103 28-29 -0.3187542880238041 1103 30-31 -0.47550141391366196 1103 32-33 -0.2636547268415512 1103 34-35 -0.19621417164030674 1103 36-37 -0.24903539537405095 1103 38-39 -0.21011305800621471 1103 40-41 -0.07918243075064169 1103 42-43 -0.2961175259298656 1103 44-45 -0.3092971273000771 1103 46-47 -0.29339968651516557 1103 48-49 -0.3400316900327809 1103 50-51 -0.13703091842717896 1103 52-53 -0.14601737770528445 1103 54-55 -0.45525338700220885 1103 56-57 -0.432968953570942 1103 58-59 -0.4837804201370446 1103 60-61 -0.4225305256080176 1103 62-63 -0.34246872826496855 1103 64-65 -0.394865939847854 1103 66-67 -0.24352642371673028 1103 68-69 -0.27559410639219095 1103 70-71 -0.32084569999813084 1103 72-73 -0.40131810129914314 1103 74-75 -0.03887244814780644 1103 76-77 0.08087979980047777 1103 78-79 0.0051456492172548 1103 80-81 -0.18062498704962238 1103 82-83 -0.19676869582083256 1103 84-85 -0.2558764109000329 1103 86-87 -0.38557769123527663 1103 88-89 -0.20189376992954777 1103 90-91 -0.2549955813568552 1103 92-93 -0.27090669454675975 1103 94-95 -0.2790748585910059 1103 96-97 -0.31162112763046323 1103 98-99 -0.11568216929168429 1103 100-101 0.3916675974384596 1104 1 0.12070754558823893 1104 2 0.10438860124840943 1104 3 -0.03664906611858498 1104 4 -0.024641048943742305 1104 5 -0.025435641662333808 1104 6 -0.014350074608934449 1104 7 0.04807890942196025 1104 8 0.045404799197903856 1104 9 0.00923226896515672 1104 10-11 0.06558387319771697 1104 12-13 0.05445364009729303 1104 14-15 0.04503656544424928 1104 16-17 -0.07923296325541429 1104 18-19 0.032365932028689315 1104 20-21 -0.0724597929421904 1104 22-23 -0.13709010189310789 1104 24-25 -0.03075763803011 1104 26-27 -0.025152536508890933 1104 28-29 -0.004448882849743541 1104 30-31 -0.02403021965371721 1104 32-33 -0.03432905656660523 1104 34-35 -0.101139830973068 1104 36-37 -0.1695218989845273 1104 38-39 0.008090191292914994 1104 40-41 -0.06626447683311909 1104 42-43 0.08668572919332007 1104 44-45 0.13684791487586523 1104 46-47 0.16009448809561633 1104 48-49 0.1337282472790875 1104 50-51 0.06436151858246575 1104 52-53 -0.016448905666543112 1104 54-55 0.25743685477592493 1104 56-57 0.020915256946089755 1104 58-59 0.06353239130706356 1104 60-61 0.12140278354662115 1104 62-63 0.13722116671654305 1104 64-65 0.05396081979208134 1104 66-67 0.04086711015859379 1104 68-69 -0.07909586993689288 1104 70-71 -0.039577527369296206 1104 72-73 0.09692078069205223 1104 74-75 0.008760686902569148 1104 76-77 0.08266319927225396 1104 78-79 0.07785822540441956 1104 80-81 0.13896994919009842 1104 82-83 0.12109358091229083 1104 84-85 0.07604480714150696 1104 86-87 0.12353410346817384 1104 88-89 0.1359510081338442 1104 90-91 0.06151767412813669 1104 92-93 0.15815199526792156 1104 94-95 0.3535503654369698 1104 96-97 0.2770621835096527 1104 98-99 0.47847289557164174 1104 100-101 1.0422212366411294 1105 1 -0.10578828792769457 1105 2 -0.1289510523927646 1105 3 -0.02907945403939749 1105 4 -0.04068628326766088 1105 5 -0.17241857414802553 1105 6 -0.14851604887395808 1105 7 -0.09741487506209978 1105 8 -0.14540290931188338 1105 9 -0.07328039326476699 1105 10-11 -0.0875155222886903 1105 12-13 -0.04580000697373521 1105 14-15 -0.125059464478241 1105 16-17 -0.13521240675230928 1105 18-19 -0.21157037357846775 1105 20-21 -0.1804008870769067 1105 22-23 -0.3093773706168008 1105 24-25 -0.32576926053959454 1105 26-27 -0.23585781131259154 1105 28-29 -0.19265080595246786 1105 30-31 -0.1947274986717602 1105 32-33 -0.20315774551713162 1105 34-35 -0.13298389705367697 1105 36-37 -0.2572662556502294 1105 38-39 -0.25540336780658635 1105 40-41 -0.11899507341353655 1105 42-43 0.04773198758599051 1105 44-45 0.08232452720278616 1105 46-47 0.03680846167016938 1105 48-49 0.11811276407515692 1105 50-51 0.2968508374112915 1105 52-53 0.226584743712003 1105 54-55 0.4191039939750425 1105 56-57 0.2082109462874584 1105 58-59 0.3494016289080051 1105 60-61 0.19185871326241255 1105 62-63 0.1787782341771802 1105 64-65 0.2691400228787799 1105 66-67 0.09458197543317937 1105 68-69 0.29079776107101196 1105 70-71 0.5227044108306131 1105 72-73 0.4123274701416406 1105 74-75 0.3620462929679178 1105 76-77 0.5376283227999856 1105 78-79 0.31241647613401113 1105 80-81 0.3572564630543802 1105 82-83 0.18815823318952596 1105 84-85 0.2895226698161295 1105 86-87 0.23605578108060143 1105 88-89 0.3310848481054407 1105 90-91 0.47512025853778184 1105 92-93 0.6789560189189885 1105 94-95 0.8109569314616785 1105 96-97 0.7104494183362213 1105 98-99 0.7105548228600753 1105 100-101 1.1443752243827099 1106 1 -0.21661840196383508 1106 2 -0.05365268660094813 1106 3 -0.10182229525171493 1106 4 -0.02604345931343488 1106 5 -0.08675871778842037 1106 6 -0.05852507618156011 1106 7 -0.08039242627215515 1106 8 -0.12729519229142738 1106 9 -0.05982904115995069 1106 10-11 -0.17196233418879814 1106 12-13 -0.19441869615556584 1106 14-15 -0.13142338445381085 1106 16-17 -0.1941852850031367 1106 18-19 -0.030863256608462564 1106 20-21 -0.007483141779736968 1106 22-23 -0.02696203481423254 1106 24-25 -0.15158370954193856 1106 26-27 -0.057113801860467106 1106 28-29 -0.21644041658344548 1106 30-31 -0.16436653912206367 1106 32-33 -0.1458525959691599 1106 34-35 -0.08194642045165779 1106 36-37 -0.12318916509837408 1106 38-39 -0.2621323627220917 1106 40-41 -0.1825761930651879 1106 42-43 -0.28009037358597055 1106 44-45 -0.2851781610763098 1106 46-47 -0.3784310938676825 1106 48-49 -0.38037217492413333 1106 50-51 -0.37737785880653263 1106 52-53 -0.298328302075042 1106 54-55 -0.38060759682241496 1106 56-57 -0.42128943206294167 1106 58-59 -0.3765996720863285 1106 60-61 -0.20596012991467205 1106 62-63 -0.14075858528993734 1106 64-65 -0.11440936844173422 1106 66-67 -0.08594094463531832 1106 68-69 -0.06595844852183319 1106 70-71 -0.09566284128350588 1106 72-73 -0.1502480339936625 1106 74-75 -0.18397638150471707 1106 76-77 -0.06001262931545881 1106 78-79 -0.019157859761484985 1106 80-81 -0.024948788714574732 1106 82-83 -0.2871378093981285 1106 84-85 0.0010653437577445857 1106 86-87 0.0838211905619275 1106 88-89 0.2103943918281388 1106 90-91 0.2141149085304832 1106 92-93 0.3078523641869495 1106 94-95 0.07018668971569042 1106 96-97 0.14943543928488268 1106 98-99 0.21573556814396966 1106 100-101 0.8638732131516775 1107 1 0.05286033925332134 1107 2 -0.008658711357220739 1107 3 0.04161580624586492 1107 4 -0.030554202158064925 1107 5 -0.0825413883985675 1107 6 -0.07894274045397509 1107 7 -0.1390027419067934 1107 8 -0.016717441638093078 1107 9 -0.079916126273325 1107 10-11 -0.07528846911204568 1107 12-13 -0.018504615439482563 1107 14-15 -0.03583710524939221 1107 16-17 -0.02852698257602526 1107 18-19 0.05048268959125579 1107 20-21 -0.12536590941193282 1107 22-23 0.001547972540670628 1107 24-25 0.017765734146145462 1107 26-27 -0.10399442561834604 1107 28-29 -0.03282325463486302 1107 30-31 -0.08816902100866741 1107 32-33 -0.10188752390681799 1107 34-35 -0.08909397220979542 1107 36-37 -0.18636792822373138 1107 38-39 -0.2187756345559606 1107 40-41 -0.12211932875521825 1107 42-43 -0.08151303762364392 1107 44-45 0.1074325144788304 1107 46-47 0.11011404485820009 1107 48-49 0.02446406124631295 1107 50-51 0.07010308887979733 1107 52-53 0.13354500590737217 1107 54-55 0.26751827363750635 1107 56-57 0.10479208993132971 1107 58-59 0.043267157327008476 1107 60-61 0.02332977040299511 1107 62-63 0.063163765773389 1107 64-65 0.11815404798628748 1107 66-67 -0.013250695351253228 1107 68-69 0.2777910624557407 1107 70-71 0.27927749798069357 1107 72-73 0.49573298411190336 1107 74-75 0.465499190847126 1107 76-77 0.518646627683431 1107 78-79 0.39077888029766683 1107 80-81 0.4556986378538177 1107 82-83 0.28699212190400303 1107 84-85 0.4541808078204639 1107 86-87 0.554276411756554 1107 88-89 0.609772159695062 1107 90-91 0.5233658435413595 1107 92-93 0.5664386199182943 1107 94-95 0.5268260493701682 1107 96-97 0.7212483823392013 1107 98-99 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22-23 0.361379128422719 2316 24-25 0.43352238013802236 2316 26-27 0.8180874597465433 2316 28-29 0.5529523799052285 2316 30-31 0.5461034756555065 2316 32-33 0.7199555383081702 2316 34-35 0.5665791315014062 2316 36-37 0.5613070743422881 2316 38-39 0.5676471868930335 2316 40-41 0.4015980278042619 2316 42-43 0.42936230512331264 2316 44-45 0.3012678346448112 2316 46-47 0.7786025857458938 2316 48-49 0.41353318249442594 2316 50-51 0.5035441950543813 2316 52-53 0.7634314411923455 2316 54-55 0.5191889359639319 2316 56-57 0.81550174109924 2316 58-59 0.8373000610352719 2316 60-61 0.7535118478528133 2316 62-63 1.0714483447261571 2316 64-65 0.7532018492737009 2316 66-67 0.7298192176973259 2316 68-69 0.6141486336811326 2316 70-71 0.7894746782912563 2316 72-73 0.7569538231271338 2316 74-75 0.8515724837106546 2316 76-77 0.6915368093576504 2316 78-79 0.3907541365330971 2316 80-81 0.5284572193579464 2316 82-83 0.728083633509744 2316 84-85 0.868632449340133 2316 86-87 0.42969064070419094 2316 88-89 0.48520746666437375 2316 90-91 0.3869076476733859 2316 92-93 0.4525980577399622 2316 94-95 0.48211681609695134 2316 96-97 0.5051225960373493 2316 98-99 0.7238025744740639 2316 100-101 0.48144031873406945 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 7.0 5 15.0 6 30.0 7 103.0 8 256.0 9 486.0 10 908.0 11 1528.0 12 2441.0 13 3441.0 14 4593.0 15 6474.0 16 9307.0 17 13657.0 18 12428.0 19 7138.0 20 6003.0 21 6079.0 22 6249.0 23 6685.0 24 7324.0 25 8694.0 26 10842.0 27 13272.0 28 16551.0 29 20781.0 30 26027.0 31 32860.0 32 42084.0 33 56277.0 34 77071.0 35 111329.0 36 172686.0 37 266737.0 38 224697.0 39 23863.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48658204350904 19.901326771811274 15.384576861954303 21.22751432272539 2 15.82848663846923 21.218139219485423 39.79199734429202 23.161376797753324 3 19.59902479467057 25.407301695599482 30.217602906598984 24.776070603130968 4 12.898366622821907 17.177359422216696 37.25698061683488 32.667293338126505 5 13.252414106036328 38.440024989761376 33.690969404224404 14.616591499977897 6 31.202984001528034 37.14404630140117 17.133486859500202 14.519482837570594 7 28.24308736061495 33.87587401067788 20.029659854186285 17.851378774520885 8 26.948513128822686 34.98970329302785 19.169474038035677 18.892309540113786 9 26.95043151084261 16.564895110377865 20.07336560107496 36.411307777704565 10-11 24.913651813082552 27.54534270283796 26.636236628646497 20.90476885543299 12-13 26.191392736179676 24.666943874574127 27.761843652237815 21.379819737008383 14-15 22.958487047168006 25.801487496736662 26.72189382673007 24.518131629365257 16-17 21.49121672962574 29.32601401086138 27.14093518621234 22.041834073300542 18-19 21.476119897208086 27.223300501198157 30.511240467518043 20.789339134075718 20-21 22.537944345235363 25.98577728252012 30.80454439678146 20.67173397546306 22-23 22.109064825009174 25.99743936209255 30.716478163680645 21.177017649217632 24-25 21.876326718196506 26.151226481899915 30.89213014745808 21.0803166524455 26-27 21.61553394819098 26.443118717014652 30.81867259977188 21.12267473502249 28-29 21.51176708552459 26.641490706025134 30.846529512592845 21.000212695857435 30-31 21.666779267726117 26.43055475622705 30.716401303503226 21.186264672543608 32-33 21.544097347044485 26.319450642115825 30.752706378286586 21.383745632553108 34-35 21.903919087650873 26.48397275230268 30.61758555775289 20.99452260229355 36-37 22.03112534421418 26.225127228399742 30.648375859571043 21.09537156781504 38-39 21.746080533346902 26.5343920088046 30.60509900789836 21.114428449950143 40-41 21.9493947082639 26.550429300890965 30.38840595666455 21.11177003418059 42-43 21.76862747060796 26.601734743417133 30.15872618648408 21.470911599490822 44-45 21.893812417869167 26.775850542840235 29.998152512395475 21.332184526895123 46-47 22.21915929827254 27.05844475935566 29.360336367435213 21.36205957493659 48-49 22.225206372030993 27.205451207621483 29.10235568971255 21.466986730634975 50-51 22.278170397363642 27.404671009994768 28.918357831627777 21.398800761013806 52-53 22.41817058086105 27.584665288211873 28.51387115312275 21.483292977804318 54-55 22.261655630409525 27.52306854378957 28.564541460822884 21.650734364978018 56-57 22.26499194696591 27.816497584923855 28.400269574377756 21.51824089373248 58-59 22.345188656190075 27.421894744217123 28.519584595225567 21.71333200436724 60-61 22.27237354734692 27.530283328342758 28.814723498594997 21.38261962571533 62-63 22.220660640722915 27.47736100696706 28.906221980153923 21.395756372156104 64-65 22.105140679957998 27.51481116031251 28.799835185962113 21.580212973767377 66-67 22.196889385258654 27.590503842185555 28.512036179016736 21.700570593539055 68-69 22.246350278207096 27.559225874469423 28.60107412711533 21.593349720208153 70-71 22.285051550261194 27.460345792529488 28.439763221613994 21.814839435595328 72-73 22.191301055026702 27.63963110347836 28.254472540863624 21.914595300631316 74-75 22.07961785830163 28.006959556336625 28.04428459781121 21.869137987550534 76-77 22.229293359812566 27.69526418205612 28.21222643246837 21.863216025662947 78-79 22.19643064173215 27.77358421321732 28.18532738023249 21.84465776481804 80-81 22.140306349054757 27.837527988579787 28.116444169012862 21.905721493352594 82-83 22.222958993664736 27.821184736820374 28.13484085111281 21.821015418402077 84-85 22.178701753654316 27.78739287278187 27.99887399128386 22.03503138227996 86-87 22.152724894843473 27.776920529773708 27.992738507015027 22.0776160683678 88-89 22.186254876235168 27.68984266765199 27.89431716860159 22.22958528751125 90-91 22.123603331567733 27.52117680915222 28.052651115457923 22.30256874382213 92-93 22.034322424264666 27.55911586956494 28.09339929960534 22.31316240656504 94-95 22.063478426960106 27.533452829071326 28.014466268588496 22.38860247538007 96-97 21.890323597683594 27.56910971226772 27.997826389763514 22.54274030028517 98-99 21.743367921219527 27.59668753672556 27.905713986637217 22.754230555417703 100-101 21.76593970753412 27.65125147386171 28.0979457485424 22.484863070061767 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 510.0 1 348.0 2 266.0 3 385.0 4 330.5 5 294.0 6 586.5 7 1319.5 8 2165.5 9 2592.5 10 2316.0 11 1949.5 12 2040.5 13 2139.5 14 2212.5 15 2332.0 16 2412.5 17 2530.5 18 2693.0 19 2855.5 20 3055.5 21 3293.5 22 3791.0 23 4594.5 24 5356.5 25 6327.5 26 7844.0 27 9612.5 28 11081.0 29 12742.0 30 14651.0 31 17006.0 32 19761.0 33 21863.0 34 23823.5 35 25857.0 36 28778.0 37 31508.0 38 32854.0 39 34223.5 40 35709.5 41 37532.0 42 38966.0 43 40256.0 44 42450.0 45 43860.0 46 44293.5 47 46100.5 48 48918.5 49 48123.5 50 44399.5 51 41239.0 52 39539.5 53 39316.5 54 38577.0 55 35403.5 56 31144.5 57 27605.5 58 25158.5 59 22618.0 60 18731.5 61 15106.0 62 12096.0 63 9069.5 64 6359.0 65 4512.0 66 3237.0 67 2477.0 68 1905.5 69 1347.0 70 940.5 71 579.5 72 383.0 73 297.0 74 222.5 75 152.0 76 97.5 77 61.0 78 40.5 79 19.0 80 10.0 81 8.0 82 5.5 83 5.0 84 2.5 85 1.5 86 1.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06931197645895038 2 0.0 3 0.0 4 0.0 5 0.003002684900748753 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.6681582781937515E-4 12-13 0.0056300341889039115 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.83855397367813E-4 24-25 8.340791390968759E-4 26-27 3.7533561259359415E-4 28-29 0.0026690532451100024 30-31 3.336316556387503E-4 32-33 0.0 34-35 0.0 36-37 4.170395695484379E-5 38-39 6.255593543226569E-4 40-41 8.340791390968757E-5 42-43 0.011093252549988449 44-45 1.2511187086453136E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.170395695484379E-5 82-83 2.0851978477421897E-4 84-85 1.6681582781937515E-4 86-87 0.0 88-89 0.0 90-91 0.007673528079691257 92-93 0.0026273492881551588 94-95 0.0 96-97 0.0 98-99 4.170395695484379E-5 100-101 0.010050653626117354 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1198927.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.27895945116914 #Duplication Level Percentage of deduplicated Percentage of total 1 78.79756730438692 47.4983536439191 2 12.434698145032467 14.991013305438806 3 3.792339612689064 6.857948572150398 4 1.631790781967301 3.9345060151599407 5 0.877207281043758 2.6438571062153513 6 0.5274370133564587 1.9076012604695836 7 0.3699875368579687 1.561172461218961 8 0.2560171727150804 1.234595901831624 9 0.19205283043426064 1.0419070300406155 >10 1.0383159800510549 11.098242348862254 >50 0.055363791769727735 2.2493317092051694 >100 0.02570251415397813 2.8043497684437786 >500 6.90925246336142E-4 0.22156871610888607 >1k 5.527401970689136E-4 0.720598106412772 >5k 2.763700985344568E-4 1.234954054522686 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8263 0.6891995926357485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6563 0.5474061389892796 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4521 0.37708717878569753 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1499 0.12502846295062167 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1339 0.11168319672507165 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1292 0.10776302477131636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 4.170395695484379E-5 0.0 0.0 0.0 16-17 0.0 8.340791390968757E-5 0.0 0.0 0.0 18-19 0.0 8.340791390968757E-5 0.0 0.0 0.0 20-21 0.0 8.340791390968757E-5 0.0 0.0 0.0 22-23 0.0 8.340791390968757E-5 0.0 0.0 0.0 24-25 0.0 1.2511187086453136E-4 0.0 0.0 0.0 26-27 0.0 1.6681582781937515E-4 0.0 8.340791390968757E-5 0.0 28-29 0.0 1.6681582781937515E-4 0.0 9.174870530065634E-4 0.0 30-31 0.0 2.502237417290627E-4 0.0 0.002543941374245471 0.0 32-33 0.0 2.502237417290627E-4 0.0 0.004378915480258597 0.0 34-35 0.0 2.502237417290627E-4 0.0 0.005796850016723287 0.0 36-37 0.0 2.919276986839065E-4 0.0 0.00825738347705907 0.0 38-39 0.0 3.336316556387503E-4 0.0 0.012344371258633762 0.0 40-41 0.0 3.336316556387503E-4 0.0 0.01814122127535705 0.0 42-43 0.0 3.753356125935941E-4 0.0 0.022186505099976897 0.0 44-45 0.0 4.170395695484379E-4 0.0 0.024605334603357835 0.0 46-47 0.0 4.170395695484379E-4 0.0 0.02873402634188737 0.0 48-49 0.0 4.170395695484379E-4 0.0 0.03461428427252035 0.0 50-51 0.0 4.170395695484379E-4 0.0 0.040953285729656605 0.0 52-53 0.0 4.170395695484379E-4 0.0 0.05801020412418771 0.0 54-55 0.0 4.170395695484379E-4 0.0 0.07102183869409898 0.0 56-57 0.0 4.170395695484379E-4 0.0 0.09917200963861852 0.0 58-59 0.0 4.170395695484379E-4 0.0 0.14963379755397951 0.0 60-61 0.0 4.170395695484379E-4 0.0 0.19388169588306878 0.0 62-63 0.0 4.170395695484379E-4 0.0 0.22007178085071066 0.0 64-65 0.0 4.170395695484379E-4 0.0 0.2614421061499157 0.0 66-67 0.0 4.170395695484379E-4 0.0 0.3527737718810236 0.0 68-69 0.0 4.170395695484379E-4 0.0 0.4818475186562652 0.0 70-71 0.0 4.170395695484379E-4 0.0 0.6024553621696733 0.0 72-73 0.0 4.170395695484379E-4 0.0 0.7350739452860766 0.0 74-75 0.0 4.170395695484379E-4 0.0 0.8991373119464321 0.0 76-77 0.0 4.170395695484379E-4 0.0 1.0117379957245103 0.0 78-79 0.0 4.170395695484379E-4 0.0 1.174383427848401 0.0 80-81 0.0 4.170395695484379E-4 0.0 1.3951224720103892 0.0 82-83 0.0 4.170395695484379E-4 0.0 1.6039758884402469 0.0 84-85 0.0 4.170395695484379E-4 0.0 1.8524063600202514 0.0 86-87 0.0 4.170395695484379E-4 0.0 2.15984793069136 0.0 88-89 0.0 4.170395695484379E-4 0.0 2.5622911153056025 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8365 0.0 33.754337 1 GGTATCA 5485 0.0 33.711807 1 CGTGTCG 65 0.0058377287 29.237368 9 TATCAAC 10455 0.0 26.850128 2 ATCAACG 10580 0.0 26.532902 3 TCAACGC 10600 0.0 26.48284 4 CAACGCA 10680 0.0 26.32894 5 AACGCAG 11045 0.0 25.543802 6 ACGCAGA 12290 0.0 22.962873 7 TATTCCG 145 2.3348963E-5 22.93047 5 CGCAGAG 12335 0.0 22.647997 8 TACATGG 6680 0.0 22.327295 2 AGTACTT 6590 0.0 22.313456 12-13 GAGTACT 6525 0.0 22.244484 12-13 GTACATG 6820 0.0 22.233887 1 TACCTGG 960 0.0 21.770296 2 GTACTTT 6760 0.0 21.359531 14-15 GCAGAGT 12990 0.0 21.323135 9 CATGGGA 4250 0.0 21.12301 4 ACATGGG 6935 0.0 20.958387 3 >>END_MODULE