FastQCFastQC Report
Thu 26 May 2016
SRR937339_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937339_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465052
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51231.10159724073867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.7601300499729063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25380.5457454220173228No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8670.1864307647316859No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.16600294160653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.13546872177734964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.050.267491
GTATCAA38700.043.48441
TATCAAC50350.033.510152
ATCAACG50550.033.1859473
TCAACGC51350.032.5728724
CAACGCA51800.032.2051165
AACGCAG52100.032.002416
GTGTAGG1102.7118367E-630.2514761
CTAGTAC650.00582693329.2447093
TACCGCA650.00583615429.2352477
ACGCAGA58950.027.6420657
CGCAGAG58600.027.3207388
AGTACCG1056.8697016E-527.158735
GCAGAGT58950.025.7079269
GTACATG34800.025.2716561
ATGGGAG7550.025.1802795
TACATGG35400.024.8379632
GAGTACT34750.024.12699512-13
ACATGGG36150.023.925653
AGTACTT35750.023.85073312-13
Produced by FastQC (version 0.11.5)