##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937339_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 465052 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.900763785555164 33.0 31.0 34.0 30.0 34.0 2 32.003272752294365 33.0 31.0 34.0 30.0 34.0 3 32.01406509379596 34.0 31.0 34.0 30.0 34.0 4 35.65893061421088 37.0 35.0 37.0 35.0 37.0 5 35.67523416736193 37.0 35.0 37.0 35.0 37.0 6 35.74322871420831 37.0 35.0 37.0 35.0 37.0 7 35.6897443726723 37.0 35.0 37.0 35.0 37.0 8 35.68134746221928 37.0 35.0 37.0 35.0 37.0 9 37.31163181751718 39.0 37.0 39.0 34.0 39.0 10-11 37.21021842718663 39.0 37.0 39.0 34.0 39.0 12-13 37.08032972656821 39.0 37.0 39.0 33.5 39.0 14-15 38.16915957785366 40.0 38.0 41.0 33.5 41.0 16-17 38.13796091619862 40.0 38.0 41.0 33.0 41.0 18-19 38.167202807427984 40.0 38.0 41.0 33.5 41.0 20-21 38.10168540292268 40.0 38.0 41.0 34.0 41.0 22-23 38.13409468188504 40.0 38.0 41.0 34.0 41.0 24-25 38.09476682177477 40.0 38.0 41.0 34.0 41.0 26-27 37.996139141429346 40.0 38.0 41.0 33.0 41.0 28-29 37.84599249116228 40.0 38.0 41.0 33.0 41.0 30-31 37.51507788376354 39.5 37.0 41.0 33.0 41.0 32-33 37.18517929177813 39.0 36.0 41.0 32.5 41.0 34-35 37.136398725303835 39.0 36.0 41.0 32.0 41.0 36-37 36.9700764645674 39.0 35.5 41.0 31.5 41.0 38-39 36.777988267978635 39.0 35.0 41.0 31.0 41.0 40-41 36.49025270292354 39.0 35.0 41.0 30.5 41.0 42-43 36.1166235173701 39.0 35.0 41.0 30.0 41.0 44-45 35.686833945451255 39.0 35.0 41.0 28.5 41.0 46-47 35.03098363193793 39.0 35.0 40.0 25.0 41.0 48-49 34.49597141825 39.0 35.0 40.0 22.0 41.0 50-51 33.45438252066435 37.5 33.5 39.5 18.5 40.5 52-53 33.50748195900673 38.0 33.5 39.5 18.0 40.5 54-55 33.77884516140131 38.0 34.0 40.0 14.5 41.0 56-57 33.51747869055504 38.0 34.0 40.0 10.0 41.0 58-59 33.244959703431014 38.0 33.0 40.0 7.5 41.0 60-61 32.91217541264203 37.5 33.0 40.0 4.0 41.0 62-63 32.53866019283865 37.0 32.5 40.0 2.0 41.0 64-65 32.11057903202222 36.0 32.0 39.5 2.0 41.0 66-67 31.674749275349853 36.0 31.0 39.0 2.0 40.5 68-69 31.16720710802233 35.0 31.0 39.0 2.0 40.0 70-71 30.694141730387138 35.0 30.5 38.0 2.0 40.0 72-73 30.211581715593095 35.0 30.0 37.5 2.0 40.0 74-75 29.66864458168119 35.0 29.0 37.0 2.0 39.0 76-77 29.180204579272853 34.0 29.0 36.5 2.0 39.0 78-79 28.66021219132484 34.0 28.5 36.0 2.0 38.5 80-81 28.107076197930553 34.0 26.5 35.5 2.0 37.0 82-83 27.597736382168016 34.0 26.0 35.0 2.0 37.0 84-85 27.077253726464996 33.0 25.5 35.0 2.0 36.0 86-87 26.520645003139435 33.0 24.5 35.0 2.0 36.0 88-89 26.012436243688878 32.5 23.5 35.0 2.0 35.5 90-91 25.48335992534168 32.0 20.0 35.0 2.0 35.0 92-93 24.875468764783292 32.0 17.5 34.5 2.0 35.0 94-95 24.15612662669981 31.5 5.0 34.0 2.0 35.0 96-97 23.362817491377307 31.0 2.0 34.0 2.0 35.0 98-99 22.239311947911204 31.0 2.0 34.0 2.0 35.0 100-101 19.269081737096066 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18892630781753894 1101 2 0.1491262728449101 1101 3 0.07770978845869791 1101 4 0.11379479103393209 1101 5 0.035634127003739025 1101 6 0.09418684674721334 1101 7 0.047767314556544704 1101 8 0.16038414889255392 1101 9 0.2330730643549046 1101 10-11 0.2692202043479881 1101 12-13 0.2885973875063925 1101 14-15 0.38800895823723636 1101 16-17 0.3249741606765397 1101 18-19 0.3234597128813519 1101 20-21 0.2904838584806342 1101 22-23 0.30950171251229364 1101 24-25 0.1632585836013405 1101 26-27 0.14539741147719099 1101 28-29 0.320903455712255 1101 30-31 0.4110660944198514 1101 32-33 0.42300306740480664 1101 34-35 0.29964201337492824 1101 36-37 0.10790149068297694 1101 38-39 0.19490600813676906 1101 40-41 0.4582286038652086 1101 42-43 0.7299670633824391 1101 44-45 0.6855332422503295 1101 46-47 0.7583291046812306 1101 48-49 0.6962005752436866 1101 50-51 0.6939206229480277 1101 52-53 0.7758316147691247 1101 54-55 0.9404221265989108 1101 56-57 1.0170714114009698 1101 58-59 1.1666414420699809 1101 60-61 1.1589867261065336 1101 62-63 1.3780816101250508 1101 64-65 1.2947791470711394 1101 66-67 1.4540141120416088 1101 68-69 1.3354395623689435 1101 70-71 1.6312555373794595 1101 72-73 1.5233247052499834 1101 74-75 1.7284535096222733 1101 76-77 1.7912296295104397 1101 78-79 1.9300615306168893 1101 80-81 2.0020758343945353 1101 82-83 1.7038097164072532 1101 84-85 1.9339188855232265 1101 86-87 2.0676764118865343 1101 88-89 2.122481572598204 1101 90-91 2.005941330293691 1101 92-93 2.5259065614587897 1101 94-95 2.451878596720917 1101 96-97 2.50377822622076 1101 98-99 3.052236457131535 1101 100-101 3.205370834852115 1102 1 0.1661150398825697 1102 2 0.12504453679829908 1102 3 -0.03435641098401021 1102 4 0.08543139558449298 1102 5 0.12818733922257763 1102 6 0.11019240666900743 1102 7 -0.05036660421403383 1102 8 -0.005447013949499535 1102 9 0.08928673162996148 1102 10-11 0.016121652136042997 1102 12-13 0.06954556202038731 1102 14-15 0.2926744860725705 1102 16-17 0.21273843558089567 1102 18-19 0.07722922294571788 1102 20-21 0.23127199038588486 1102 22-23 0.23488704237283997 1102 24-25 0.2004386120504762 1102 26-27 0.1262377650778177 1102 28-29 0.16068417655351652 1102 30-31 0.43922294137261275 1102 32-33 0.24876447141497238 1102 34-35 0.4055461826667468 1102 36-37 0.3401646256734665 1102 38-39 0.2581468709638841 1102 40-41 0.28734804475531206 1102 42-43 0.7021304315518861 1102 44-45 0.851830763018846 1102 46-47 0.5874888160329519 1102 48-49 0.9764043957413477 1102 50-51 0.8771531718733883 1102 52-53 1.049885746063815 1102 54-55 1.3257376976275665 1102 56-57 1.3276969675555321 1102 58-59 1.2828178266918613 1102 60-61 1.3511658127464798 1102 62-63 1.4910253159168576 1102 64-65 1.225569162594752 1102 66-67 1.3768338237830484 1102 68-69 1.5797688968021397 1102 70-71 1.6267413485369744 1102 72-73 1.6766038250156328 1102 74-75 1.70542465128986 1102 76-77 1.6320710223487929 1102 78-79 1.616994415345939 1102 80-81 1.910910654054316 1102 82-83 2.0332480733724196 1102 84-85 2.188865332304509 1102 86-87 2.122132468369106 1102 88-89 2.2918331532138225 1102 90-91 2.368640490459711 1102 92-93 2.2967975129458758 1102 94-95 2.1919275707984305 1102 96-97 2.3661967922026115 1102 98-99 2.307873996996044 1102 100-101 2.750318515728189 1103 1 0.13964024448185341 1103 2 0.022554952406469653 1103 3 0.19269368555089272 1103 4 0.2618487390377098 1103 5 0.28065933628353434 1103 6 0.2323078889917909 1103 7 0.08675360714659774 1103 8 0.1459009572541774 1103 9 0.04042834481811042 1103 10-11 0.15403837417022714 1103 12-13 0.006919030945532256 1103 14-15 0.09919196006286768 1103 16-17 0.197112287519019 1103 18-19 0.06016013209347193 1103 20-21 0.10764008931266034 1103 22-23 -0.1629094604841228 1103 24-25 0.19250555334005526 1103 26-27 -0.11894838704868249 1103 28-29 -0.08186934755676134 1103 30-31 -0.11863546888900345 1103 32-33 0.18935212276372226 1103 34-35 0.08054168812663676 1103 36-37 0.16573347811966244 1103 38-39 0.45935766098678954 1103 40-41 0.35375546113732526 1103 42-43 0.01039256876433825 1103 44-45 0.14861110898118568 1103 46-47 0.18412377339344488 1103 48-49 0.4977882451000042 1103 50-51 0.44747435561799875 1103 52-53 0.7555895128792613 1103 54-55 1.086455417058822 1103 56-57 0.853200942292311 1103 58-59 0.9879462923731168 1103 60-61 0.8733149064126948 1103 62-63 1.0747183427355793 1103 64-65 0.7127108695820112 1103 66-67 0.8390773418745461 1103 68-69 1.0966496077992964 1103 70-71 0.9935622626091813 1103 72-73 1.234529457964129 1103 74-75 1.090313616607645 1103 76-77 1.2335796245077368 1103 78-79 1.03935612030088 1103 80-81 1.3031581586074807 1103 82-83 1.2802640895683979 1103 84-85 1.1591536863648386 1103 86-87 1.33148423843625 1103 88-89 1.5177764203927353 1103 90-91 0.9202370292730002 1103 92-93 1.1055278639889998 1103 94-95 1.3501127145846326 1103 96-97 1.314965288212246 1103 98-99 1.7308344528596606 1103 100-101 1.1559941428142437 1104 1 0.2810421429143517 1104 2 0.38456645817588964 1104 3 0.1993356701307505 1104 4 0.32711294955787906 1104 5 0.346254284172943 1104 6 0.3278131681438552 1104 7 0.26421366088169407 1104 8 0.2271592378478715 1104 9 0.13618654078194936 1104 10-11 0.35666073240549423 1104 12-13 0.35188297084472 1104 14-15 0.4425372323667176 1104 16-17 0.3657941823717721 1104 18-19 0.38346271982156566 1104 20-21 0.43662357773587956 1104 22-23 0.5565347840047181 1104 24-25 0.4219896224991828 1104 26-27 0.4170300021825142 1104 28-29 0.3779528713797049 1104 30-31 0.5054533010208928 1104 32-33 0.7207507721879409 1104 34-35 0.7057082727574695 1104 36-37 0.6289856381265082 1104 38-39 0.7214543802666356 1104 40-41 0.7753298488354545 1104 42-43 0.9618344864185175 1104 44-45 0.9607143457327254 1104 46-47 1.1001301002047654 1104 48-49 0.9666999051486798 1104 50-51 0.8136356426143863 1104 52-53 0.8230171348970288 1104 54-55 1.095424236306961 1104 56-57 1.0887238867109161 1104 58-59 1.2254449061944328 1104 60-61 1.1790426939937007 1104 62-63 1.5814663578249863 1104 64-65 1.4499332204243203 1104 66-67 1.527999390967647 1104 68-69 1.5574309651065512 1104 70-71 1.4616665343741353 1104 72-73 1.589795866915047 1104 74-75 1.6015606326756426 1104 76-77 1.551584577786219 1104 78-79 1.7013901940319656 1104 80-81 1.5205070845865123 1104 82-83 1.50338340988365 1104 84-85 1.6004896318748862 1104 86-87 1.5036019109011995 1104 88-89 1.5861242705025624 1104 90-91 1.4212700971733803 1104 92-93 1.613701940793998 1104 94-95 1.6494329520151503 1104 96-97 1.4544975477515827 1104 98-99 1.9422972264232428 1104 100-101 2.319521398491741 1105 1 0.05143289493779335 1105 2 -0.32124262491347366 1105 3 0.11167745116770078 1105 4 0.10872675107555807 1105 5 0.15309888947741257 1105 6 0.05572377171777276 1105 7 0.22658313582062561 1105 8 0.06594244181537334 1105 9 0.2995397235387287 1105 10-11 0.21428599945382842 1105 12-13 0.3289314900505005 1105 14-15 0.2859594839702879 1105 16-17 0.3309011420918253 1105 18-19 0.1909658529335161 1105 20-21 0.26920436863287733 1105 22-23 0.12906217799578457 1105 24-25 0.27757294285764544 1105 26-27 0.33944113283391175 1105 28-29 0.1578425041611169 1105 30-31 0.4495805347191393 1105 32-33 0.5213865833385114 1105 34-35 0.5638963513622031 1105 36-37 0.5557410103606344 1105 38-39 0.5755968519920529 1105 40-41 0.6424463393416815 1105 42-43 0.5175745261854345 1105 44-45 0.28553191055814864 1105 46-47 0.6700654981163652 1105 48-49 0.892433027821177 1105 50-51 1.013959546408067 1105 52-53 0.8988371679255138 1105 54-55 1.097546584647752 1105 56-57 0.9569340949129099 1105 58-59 0.8706081725944514 1105 60-61 0.965659264901582 1105 62-63 0.8364110593561946 1105 64-65 0.8593205598443561 1105 66-67 1.1002881324393279 1105 68-69 0.8177689583323939 1105 70-71 1.1212497730547284 1105 72-73 0.9883991753791719 1105 74-75 0.8670902753604963 1105 76-77 0.9645836104139747 1105 78-79 0.9193233355924697 1105 80-81 0.9628565484478848 1105 82-83 0.9538330210204258 1105 84-85 0.9859785377232768 1105 86-87 0.9426076870578974 1105 88-89 1.0658694355556655 1105 90-91 1.1848075656189216 1105 92-93 1.3193668113983854 1105 94-95 1.1953537806371202 1105 96-97 1.0297062729897881 1105 98-99 1.0899861256353063 1105 100-101 0.8755715589121777 1106 1 0.2041502327716458 1106 2 -0.5478476365305553 1106 3 0.03175040796123696 1106 4 0.27903621199750717 1106 5 0.13233193496945717 1106 6 0.04071616574500325 1106 7 0.07234081936675096 1106 8 0.23251324547532448 1106 9 0.12683873287024028 1106 10-11 -0.3514735004440013 1106 12-13 -0.20821857844109815 1106 14-15 -0.10258274242281118 1106 16-17 0.10661197890019025 1106 18-19 0.06544270580145906 1106 20-21 -0.010542174622777623 1106 22-23 -0.009321117623024122 1106 24-25 0.2745116334194364 1106 26-27 0.025838550587238274 1106 28-29 0.2183648566393046 1106 30-31 0.2313988541397194 1106 32-33 0.11742807183873083 1106 34-35 0.32061685267527906 1106 36-37 0.19142815891549958 1106 38-39 0.3049415274327103 1106 40-41 0.2650462954465098 1106 42-43 -0.1112710309426177 1106 44-45 0.24435937985045086 1106 46-47 0.3519806896279434 1106 48-49 0.6546271806823114 1106 50-51 0.06550231013854813 1106 52-53 0.19082138080194255 1106 54-55 0.5781097293580117 1106 56-57 0.5772409069886208 1106 58-59 -0.024056672675079938 1106 60-61 -0.13958507224356964 1106 62-63 0.04447225527242793 1106 64-65 0.3232281378150361 1106 66-67 0.407088855952761 1106 68-69 0.483484563063989 1106 70-71 0.357239268487362 1106 72-73 0.22222796909459674 1106 74-75 -0.11432806827811248 1106 76-77 0.1643595937035407 1106 78-79 0.1479755269451246 1106 80-81 0.3182011633434847 1106 82-83 0.12686322543567385 1106 84-85 0.036280127787833294 1106 86-87 -0.18417744610338715 1106 88-89 -0.18077220582082631 1106 90-91 -0.04332431665121206 1106 92-93 0.4090775573891712 1106 94-95 0.02477943509134306 1106 96-97 0.23093155357829076 1106 98-99 0.1998716197964896 1106 100-101 -0.12337079462554712 1107 1 0.0678172008188902 1107 2 0.2617439607223915 1107 3 0.11742264696244575 1107 4 0.14813764900576132 1107 5 0.09076065205003658 1107 6 0.26531843956529144 1107 7 0.16901665569785962 1107 8 -0.10794421177031666 1107 9 0.017639571605975846 1107 10-11 -0.021822460681001132 1107 12-13 0.17565691931778815 1107 14-15 0.10654331802001593 1107 16-17 0.2095687147561378 1107 18-19 0.18073520527244114 1107 20-21 0.11321943975115545 1107 22-23 0.19961738150168884 1107 24-25 0.3038481472797301 1107 26-27 0.4508044048088138 1107 28-29 0.13340919805861517 1107 30-31 0.2039571905189277 1107 32-33 0.35355815282674286 1107 34-35 0.6204264057984119 1107 36-37 0.6692382122021456 1107 38-39 0.9148668876466246 1107 40-41 1.152692256626132 1107 42-43 0.9794653636479254 1107 44-45 1.1878120470685758 1107 46-47 1.4318192907090719 1107 48-49 1.4909679074583764 1107 50-51 1.2659236017146398 1107 52-53 1.3356571925096077 1107 54-55 1.4454076921535375 1107 56-57 1.5434269678161954 1107 58-59 1.4188525476469493 1107 60-61 1.5978579379713054 1107 62-63 1.1489535279961984 1107 64-65 1.1123611235988449 1107 66-67 1.4407873727755636 1107 68-69 1.5442880949878877 1107 70-71 1.4953517283300037 1107 72-73 1.7509488970902893 1107 74-75 1.8227186585364237 1107 76-77 1.9418108758534736 1107 78-79 2.2027867152182097 1107 80-81 1.8695400625990075 1107 82-83 1.672190543982122 1107 84-85 1.5820931820555728 1107 86-87 1.5696795840795552 1107 88-89 1.382248397068576 1107 90-91 1.238844854039204 1107 92-93 1.1135819145828698 1107 94-95 1.458854036403313 1107 96-97 1.4548316747717571 1107 98-99 1.5760907683642884 1107 100-101 1.7257192925183276 1108 1 0.019918271326297088 1108 2 0.1412262825137205 1108 3 0.29370257666047195 1108 4 0.18395057270072357 1108 5 0.06310777948378643 1108 6 0.04706275035481866 1108 7 0.11647277402430944 1108 8 0.19049681902103544 1108 9 0.41083990942068027 1108 10-11 0.1482889268700731 1108 12-13 0.2128450684127614 1108 14-15 0.16938141804055817 1108 16-17 0.21247323645160776 1108 18-19 0.05940722828036371 1108 20-21 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-1.1122924958243559 2316 62-63 -0.4828203211467894 2316 64-65 -0.27502513729413636 2316 66-67 -0.22173210474592864 2316 68-69 -0.14096958540762827 2316 70-71 0.2699030239458793 2316 72-73 0.2003939079592243 2316 74-75 -0.29882588487199513 2316 76-77 -0.7570377862091249 2316 78-79 -0.808356350784134 2316 80-81 -0.6981743825080464 2316 82-83 -0.549992669760833 2316 84-85 -0.36983340933007014 2316 86-87 -0.15142635440033203 2316 88-89 0.18604002125340813 2316 90-91 0.47741804142738786 2316 92-93 0.7616976447254125 2316 94-95 1.0160458106371948 2316 96-97 0.8248180164603873 2316 98-99 1.6321860302768414 2316 100-101 1.908288594020739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1915.0 3 272.0 4 204.0 5 231.0 6 300.0 7 343.0 8 477.0 9 656.0 10 792.0 11 1175.0 12 1855.0 13 2814.0 14 3265.0 15 4563.0 16 6555.0 17 9300.0 18 7349.0 19 4154.0 20 3794.0 21 3904.0 22 4185.0 23 4478.0 24 4893.0 25 5585.0 26 6327.0 27 7106.0 28 8378.0 29 9889.0 30 11903.0 31 14686.0 32 18846.0 33 24623.0 34 33015.0 35 46578.0 36 63786.0 37 81982.0 38 58850.0 39 6024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.508926413204875 19.808392900307332 15.703235608964397 20.979445077523394 2 14.73539575235441 21.42222356438121 41.13132498414574 22.71105569911863 3 17.760095595943213 27.62468238938091 31.46944251517413 23.145779499501746 4 11.522608003554643 17.250371533522138 38.94210547885225 32.28491498407097 5 11.84134180879311 38.629632905030704 34.360770729034776 15.168254557141408 6 28.49288842031732 38.00625672403793 18.181582981365658 15.319271874279087 7 25.662209535472353 35.571744576630024 21.682053183737004 17.08399270416061 8 25.962411251183138 33.84302590074794 20.487097059349864 19.707465788719063 9 27.061459404791936 15.916349137549949 21.15358489833677 35.86860655932134 10-11 23.581674990727084 27.884955446868343 27.553718224085433 20.979651338319144 12-13 25.397130011967267 25.81285780513838 28.514118141731267 20.275894041163088 14-15 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26.966132291526336 32.87705779334501 20.392078674390408 40-41 19.62080088112658 27.157364509005788 32.87369477197509 20.348139837892536 42-43 19.695697491056425 27.25885222595043 32.25776897282813 20.78768131016501 44-45 19.640638017872174 27.52802025412503 31.976447789474367 20.85489393852843 46-47 19.87318657010851 27.74879212471784 31.270980163033517 21.107041142140133 48-49 20.04775971365734 28.13747897710121 30.514360257018414 21.300401052223037 50-51 20.258246083177884 28.44545562372629 30.050139636191115 21.24615865690471 52-53 20.186644550304838 28.75810085449253 29.71958020614376 21.335674389058873 54-55 20.12165080268615 28.656073430339173 29.724237337654287 21.49803842932039 56-57 20.285224086164273 28.781595470211297 29.410002349386033 21.5231780942384 58-59 20.265125596300916 28.113399273501553 29.932016038568428 21.689459091629104 60-61 20.194141696713945 28.169619669347036 30.160822084911132 21.47541654902789 62-63 20.264009543915915 28.048077368655306 30.57857296041366 21.109340127015123 64-65 20.282413488778566 27.97464657904463 30.45923441892843 21.283705513248368 66-67 20.222212643058818 28.009677349828653 30.173502597150677 21.594607409961853 68-69 20.23589124648426 28.019116997316722 30.119292649546857 21.625699106652156 70-71 20.178398423145026 27.95490574110136 30.16543398536301 21.701261850390605 72-73 20.051471230104205 27.968082664347442 29.914419380599355 22.066026724948998 74-75 19.951567117370526 28.01153594961428 29.845928684897434 22.190968248117755 76-77 19.89779420007416 27.91613131321842 30.099295489234006 22.08677899747342 78-79 19.854786275405935 27.644610104598723 29.896867212224166 22.603736407771176 80-81 19.674314986921082 27.819889225277507 29.685211391323186 22.820584396478232 82-83 19.900982736639726 27.485049880431372 29.82749571891512 22.786471664013778 84-85 19.69474120885929 27.048967475338475 29.93497222716081 23.32131908864142 86-87 19.714114838980816 26.967386677729944 30.05220933302442 23.266289150264818 88-89 19.651016924310483 26.577439713071293 29.957452128255753 23.814091234362472 90-91 19.23303237153443 26.40718859721914 30.506706440467546 23.853072590778886 92-93 19.17023672879922 26.42309412569852 30.50832295994525 23.89834618555701 94-95 18.76947216753042 26.675913083680076 30.225976827918576 24.32863792087093 96-97 18.842446294388232 26.93482468884587 29.926840519229692 24.295888497536204 98-99 19.007749598508283 27.052350208560128 29.62987313939577 24.31002705353582 100-101 19.184999595894286 26.527762062555567 29.952315525741536 24.334922815808614 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 935.0 1 567.5 2 237.5 3 276.0 4 245.0 5 250.5 6 446.5 7 863.0 8 1436.5 9 1727.0 10 1515.5 11 1319.5 12 1348.5 13 1416.5 14 1488.5 15 1527.0 16 1523.5 17 1611.5 18 1703.0 19 1785.5 20 1960.0 21 2122.5 22 2354.5 23 2674.0 24 2954.5 25 3349.5 26 3853.0 27 4470.0 28 5148.0 29 5963.5 30 6841.5 31 7813.0 32 8823.5 33 9728.5 34 10757.5 35 11716.5 36 13033.0 37 14116.0 38 14731.5 39 15156.0 40 15244.0 41 15493.5 42 15699.0 43 16303.5 44 17015.5 45 17450.0 46 17322.0 47 16942.0 48 16845.5 49 16261.5 50 15051.5 51 13651.0 52 12387.0 53 11594.5 54 11181.0 55 10477.0 56 9414.0 57 8305.0 58 7561.0 59 6825.0 60 5875.0 61 5051.0 62 4150.0 63 2981.5 64 1984.0 65 1368.5 66 940.5 67 666.0 68 464.0 69 308.5 70 230.0 71 176.5 72 128.0 73 98.5 74 76.5 75 56.0 76 50.5 77 34.5 78 15.0 79 14.5 80 10.5 81 3.5 82 3.5 83 3.0 84 3.5 85 2.5 86 2.0 87 2.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36727075681859234 2 0.31265320867343865 3 0.3083526143313006 4 0.30813758461419366 5 0.3094277629168351 6 0.26470158175859904 7 0.2636264331730645 8 0.2672819383638819 9 0.28276407799557896 10-11 0.28727970205482395 12-13 0.27674324591658567 14-15 0.26330388859740417 16-17 0.2587882645381592 18-19 0.24599399637029837 20-21 0.25104719472231063 22-23 0.24921944212690195 24-25 0.25126222443941754 26-27 0.24567145179463803 28-29 0.26835708694941646 30-31 0.284914375166648 32-33 0.2752380378968374 34-35 0.2735178001599821 36-37 0.2542726404789142 38-39 0.23965061971564472 40-41 0.23664020367614802 42-43 0.2519073135907382 44-45 0.24653157066306564 46-47 0.26255128458752997 48-49 0.2735178001599821 50-51 0.2896450289429999 52-53 0.31039539664381616 54-55 0.2721201069987872 56-57 0.23674771853470147 58-59 0.2248135692352683 60-61 0.22814652985042533 62-63 0.2347049362221859 64-65 0.2261037475379097 66-67 0.23266215390967032 68-69 0.22943670815306674 70-71 0.234489906505079 72-73 0.23728529282746874 74-75 0.23889801570577054 76-77 0.2545951850545745 78-79 0.2545951850545745 80-81 0.2445963032091035 82-83 0.2349199659392928 84-85 0.2192227965904888 86-87 0.22674883668923046 88-89 0.23739280768602222 90-91 0.23545754023206006 92-93 0.24180091688671373 94-95 0.2340598470708652 96-97 0.24384369919922935 98-99 0.24878938269268813 100-101 0.22889913386029948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 465052.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.45389311073591 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24959502554043 45.73993463581313 2 12.098787986065828 14.144425194138954 3 4.065513613468504 7.129352946057889 4 1.8503680912967515 4.32644874496707 5 0.9679368052594802 2.828983727629243 6 0.5989030353124225 2.1004928405908583 7 0.39353831644239956 1.6102692679012094 8 0.30191890425651474 1.411866828601666 9 0.22599336836710643 1.1889172978439406 >10 1.1620242674513575 12.16579571824623 >50 0.06369724775432735 2.5320946574852092 >100 0.019514185688126434 1.9234449568635243 >500 0.0011045765483845152 0.48834022682369216 >1k 7.363843655896768E-4 1.307047244380909 >5k 3.681921827948384E-4 1.1025857126565777 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5123 1.10159724073867 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3535 0.7601300499729063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2538 0.5457454220173228 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 867 0.1864307647316859 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.16600294160653 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 630 0.13546872177734964 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.1502971710690418E-4 0.0 24-25 0.0 0.0 0.0 2.1502971710690418E-4 0.0 26-27 0.0 0.0 0.0 0.0015052080197483293 0.0 28-29 0.0 0.0 0.0 0.0051607132105657 0.0 30-31 0.0 0.0 0.0 0.011181545289559017 0.0 32-33 0.0 0.0 0.0 0.019245159681067922 0.0 34-35 0.0 0.0 0.0 0.02462090260874053 0.0 36-37 0.0 0.0 0.0 0.035479903322639186 0.0 38-39 0.0 0.0 0.0 0.05031695380301558 0.0 40-41 0.0 0.0 0.0 0.07171241065515255 0.0 42-43 0.0 0.0 0.0 0.1104177597343953 0.0 44-45 0.0 0.0 0.0 0.1409519795635757 0.0 46-47 0.0 0.0 0.0 0.20191290436338302 0.0 48-49 0.0 0.0 0.0 0.28125886997583066 0.0 50-51 0.0 0.0 0.0 0.33114576434463244 0.0 52-53 0.0 0.0 0.0 0.41973800779267695 0.0 54-55 0.0 0.0 0.0 0.5615501062246803 0.0 56-57 0.0 0.0 0.0 0.7624953768610823 0.0 58-59 0.0 0.0 0.0 0.9812881140173572 0.0 60-61 0.0 0.0 0.0 1.255235973611553 0.0 62-63 0.0 0.0 0.0 1.5371399327387045 0.0 64-65 0.0 0.0 0.0 1.8116253666256676 0.0 66-67 0.0 0.0 0.0 2.1867447081186624 0.0 68-69 0.0 0.0 0.0 2.6899142461488177 0.0 70-71 0.0 0.0 0.0 3.0072981085986084 0.0 72-73 0.0 0.0 0.0 3.437250027953863 0.0 74-75 0.0 0.0 0.0 3.922572099464146 0.0 76-77 0.0 0.0 0.0 4.415312696214617 0.0 78-79 0.0 0.0 0.0 5.005031695380302 0.0 80-81 0.0 0.0 0.0 5.848915820166347 0.0 82-83 0.0 0.0 0.0 6.726237065962517 0.0 84-85 0.0 0.0 0.0 7.78375321469427 0.0 86-87 0.0 0.0 0.0 9.08274773573708 0.0 88-89 0.0 0.0 0.0 10.555808812777926 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2090 0.0 50.26749 1 GTATCAA 3870 0.0 43.4844 1 TATCAAC 5035 0.0 33.51015 2 ATCAACG 5055 0.0 33.185947 3 TCAACGC 5135 0.0 32.572872 4 CAACGCA 5180 0.0 32.205116 5 AACGCAG 5210 0.0 32.00241 6 GTGTAGG 110 2.7118367E-6 30.251476 1 CTAGTAC 65 0.005826933 29.244709 3 TACCGCA 65 0.005836154 29.235247 7 ACGCAGA 5895 0.0 27.642065 7 CGCAGAG 5860 0.0 27.320738 8 AGTACCG 105 6.8697016E-5 27.15873 5 GCAGAGT 5895 0.0 25.707926 9 GTACATG 3480 0.0 25.271656 1 ATGGGAG 755 0.0 25.180279 5 TACATGG 3540 0.0 24.837963 2 GAGTACT 3475 0.0 24.126995 12-13 ACATGGG 3615 0.0 23.92565 3 AGTACTT 3575 0.0 23.850733 12-13 >>END_MODULE