FastQCFastQC Report
Thu 26 May 2016
SRR937339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937339_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465052
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54481.171481898798414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41880.9005444552437146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30840.6631516475576925No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.20427823125155897No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.16600294160653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5530.118911433560118No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.050.2351951
GTATCAA36900.043.893131
ATCAACG50750.031.8208223
TATCAAC50700.031.758522
TCAACGC51500.031.449644
CAACGCA52500.030.8506015
AACGCAG53950.030.0214376
ACGCAGA61000.026.3988577
CGCAGAG61200.026.1573498
ATCCTAG1701.1204429E-725.1456071
GCAGAGT63450.024.7057239
GTACATG35700.024.2142891
AGTACTT39400.023.26892512-13
TACATGG36050.023.0569212
ACTATAC1252.25398E-422.7986853
TGGTATC10050.022.6852592
GTATACT1050.002195005522.6177434
GATTAGC1050.002195005522.6177431
ACATGGG36750.022.230013
AGAGTAC59600.022.19699310-11
Produced by FastQC (version 0.11.5)