##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937339_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 465052 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26111058548291 33.0 31.0 34.0 31.0 34.0 2 32.44804021915829 34.0 31.0 34.0 31.0 34.0 3 32.491781564212175 34.0 31.0 34.0 31.0 34.0 4 36.138227553047834 37.0 35.0 37.0 35.0 37.0 5 36.08214350223201 37.0 35.0 37.0 35.0 37.0 6 36.138167344727044 37.0 35.0 37.0 35.0 37.0 7 36.078369730696785 37.0 35.0 37.0 35.0 37.0 8 36.064065953914834 37.0 35.0 37.0 35.0 37.0 9 37.757960400127295 39.0 37.0 39.0 35.0 39.0 10-11 37.684829868487824 39.0 37.0 39.0 35.0 39.0 12-13 37.572326320497496 39.0 37.0 39.0 35.0 39.0 14-15 38.72628544764886 40.0 38.0 41.0 34.5 41.0 16-17 38.69994000670893 40.0 38.0 41.0 34.5 41.0 18-19 38.778288234434 40.0 38.0 41.0 35.0 41.0 20-21 38.731494542545775 40.0 38.0 41.0 35.0 41.0 22-23 38.66145291279255 40.0 38.0 41.0 34.5 41.0 24-25 38.66515894996689 40.0 38.0 41.0 34.0 41.0 26-27 38.488905541745865 40.0 38.0 41.0 34.0 41.0 28-29 38.31820742626631 40.0 38.0 41.0 34.0 41.0 30-31 38.24590905963204 40.0 37.5 41.0 34.0 41.0 32-33 38.037604396927655 40.0 37.0 41.0 34.0 41.0 34-35 37.8588641700283 40.0 37.0 41.0 33.0 41.0 36-37 37.65390967031644 40.0 36.0 41.0 33.0 41.0 38-39 37.49050106224681 40.0 36.0 41.0 33.0 41.0 40-41 37.22632952874087 40.0 36.0 41.0 33.0 41.0 42-43 36.914360114567835 40.0 35.5 41.0 32.5 41.0 44-45 36.41414938544507 39.0 35.0 41.0 30.5 41.0 46-47 35.76280932024806 39.0 35.0 41.0 29.0 41.0 48-49 35.22015064981981 39.0 35.0 41.0 26.0 41.0 50-51 34.88720831218875 39.0 35.0 40.5 24.0 41.0 52-53 34.59471198919691 39.0 35.0 40.0 23.0 41.0 54-55 34.34191552772593 38.0 34.5 40.0 22.5 41.0 56-57 34.0341746729398 38.0 34.0 40.0 20.5 41.0 58-59 33.740737594935624 38.0 34.0 40.0 18.5 41.0 60-61 33.38398071613497 37.5 33.5 40.0 16.0 41.0 62-63 33.00377054608947 37.0 33.0 40.0 10.5 41.0 64-65 32.58621939051977 36.0 33.0 39.0 7.5 41.0 66-67 32.13522036245409 36.0 32.0 39.0 4.0 40.0 68-69 31.674094509861263 35.0 31.5 39.0 2.0 40.0 70-71 31.228926012574938 35.0 31.0 38.0 2.0 40.0 72-73 30.749295777676473 35.0 31.0 37.5 2.0 39.5 74-75 30.273837119289887 35.0 30.0 37.0 2.0 39.0 76-77 28.97893568891221 33.5 28.5 35.5 2.0 38.0 78-79 29.572882172316213 34.0 30.0 36.0 2.0 38.0 80-81 29.383315199160524 34.0 30.0 35.5 2.0 37.0 82-83 29.004046859275952 34.0 29.0 35.0 2.0 37.0 84-85 28.602845488246476 34.0 29.0 35.0 2.0 36.5 86-87 28.27112989515151 34.0 29.0 35.0 2.0 36.0 88-89 27.882565820596405 34.0 28.0 35.0 2.0 36.0 90-91 27.384812881140174 33.5 26.5 35.0 2.0 35.0 92-93 26.92388163044133 33.0 25.5 35.0 2.0 35.0 94-95 26.50474785615372 33.0 25.0 35.0 2.0 35.0 96-97 25.91693186998443 33.0 24.5 35.0 2.0 35.0 98-99 25.065157229729145 32.0 19.0 34.0 2.0 35.0 100-101 22.402795816381825 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21256719993591844 1101 2 0.3037789882043356 1101 3 0.22988728076439457 1101 4 0.09377544984301522 1101 5 0.1510841822572928 1101 6 0.1898534886270582 1101 7 0.25272629801758484 1101 8 0.18330263450519624 1101 9 0.1870957847820165 1101 10-11 0.19758727343545957 1101 12-13 0.21478383898334386 1101 14-15 0.3137649026892291 1101 16-17 0.24464393998457723 1101 18-19 0.2540997322354954 1101 20-21 0.23173889303308925 1101 22-23 0.280300984777746 1101 24-25 0.2091371403944109 1101 26-27 0.09066034210743368 1101 28-29 0.16747925086131943 1101 30-31 0.4488065757126236 1101 32-33 0.5339937087806348 1101 34-35 0.4684897285001739 1101 36-37 0.5693086067703064 1101 38-39 0.5333118197278921 1101 40-41 0.8022010675795528 1101 42-43 0.8110769830708477 1101 44-45 1.0308564007133754 1101 46-47 1.266465648287273 1101 48-49 1.4425341906545057 1101 50-51 1.4849685018454082 1101 52-53 1.420863557648623 1101 54-55 1.6045125655677381 1101 56-57 1.6679716286750192 1101 58-59 1.7431646376174328 1101 60-61 1.696400359770628 1101 62-63 1.6474750329170647 1101 64-65 1.5214391848821904 1101 66-67 1.7078952482476772 1101 68-69 1.8165804451359833 1101 70-71 1.8368115215414171 1101 72-73 1.6790156093411994 1101 74-75 1.8891091482624063 1101 76-77 1.9852651263056913 1101 78-79 1.8555847184793173 1101 80-81 1.7207723911617734 1101 82-83 1.4383328444328356 1101 84-85 1.5692612845376814 1101 86-87 1.9159187729581788 1101 88-89 1.967411723117035 1101 90-91 2.042571997641925 1101 92-93 2.147639469654937 1101 94-95 2.1766286270248223 1101 96-97 2.267007475347711 1101 98-99 2.3965296280415167 1101 100-101 2.879874623626826 1102 1 0.11539847319698282 1102 2 0.12853363067408452 1102 3 0.056237153133011475 1102 4 0.06018988485493537 1102 5 0.025791084744312798 1102 6 0.040581279792235136 1102 7 -0.02159478746621346 1102 8 -0.0661650809255363 1102 9 0.08535701017319042 1102 10-11 -0.030829382947082706 1102 12-13 0.11395634594948234 1102 14-15 0.1546537107485193 1102 16-17 0.15937903179473523 1102 18-19 0.14278892870988358 1102 20-21 -0.07878208982598522 1102 22-23 -0.025297258910505604 1102 24-25 -0.10072197794229254 1102 26-27 0.08182682149480769 1102 28-29 -0.12031620117461017 1102 30-31 -0.030973755414493098 1102 32-33 0.052071898428522445 1102 34-35 0.19236881319155685 1102 36-37 0.312596105390071 1102 38-39 0.19564075145646598 1102 40-41 0.30089162805344216 1102 42-43 0.4410830236400898 1102 44-45 0.5816114718686833 1102 46-47 0.3964327174420532 1102 48-49 0.6347236696716507 1102 50-51 0.6006174733248244 1102 52-53 0.8097294245352629 1102 54-55 0.8365152416048645 1102 56-57 0.8391003057903532 1102 58-59 0.9399384540230997 1102 60-61 0.9494969634118675 1102 62-63 1.053164859429323 1102 64-65 1.0698189483256968 1102 66-67 1.0534326965293523 1102 68-69 1.2914523266109228 1102 70-71 1.3414997827018489 1102 72-73 1.3193042576176879 1102 74-75 1.2596396141656676 1102 76-77 1.1649662155567242 1102 78-79 1.3194731563101705 1102 80-81 1.3970920606841801 1102 82-83 1.6310172604137136 1102 84-85 1.6236770705586352 1102 86-87 1.3229108942372179 1102 88-89 1.305976535826911 1102 90-91 1.4658281892254017 1102 92-93 1.4032568742886404 1102 94-95 1.3439322598101704 1102 96-97 1.731661051949274 1102 98-99 1.9126059860329292 1102 100-101 2.342921928428755 1103 1 0.11492166111565894 1103 2 0.09216459375632269 1103 3 0.10477036973858134 1103 4 0.08159640232892684 1103 5 -0.15807859704224114 1103 6 -0.04966439567851921 1103 7 0.009393307864932865 1103 8 -0.033166558261328305 1103 9 -0.0765699359626808 1103 10-11 -0.04705584523593842 1103 12-13 -0.15835337867199684 1103 14-15 -0.22306587684811774 1103 16-17 -0.045246827053261995 1103 18-19 -0.05122981547652472 1103 20-21 -0.03300422172315365 1103 22-23 -0.026882463666126455 1103 24-25 0.0027009074742991857 1103 26-27 0.009968895861860005 1103 28-29 -0.13010335538814388 1103 30-31 -0.021867426320362426 1103 32-33 -0.006326638305544918 1103 34-35 0.12491631852638108 1103 36-37 0.13119568485744537 1103 38-39 0.26632618836485733 1103 40-41 0.38993980170683784 1103 42-43 0.5576581059097165 1103 44-45 0.5153197410408339 1103 46-47 0.5690879354077012 1103 48-49 0.6095534633917978 1103 50-51 0.7958728022860768 1103 52-53 0.774954972282913 1103 54-55 0.9016485535728478 1103 56-57 1.030906954583081 1103 58-59 1.036170856921494 1103 60-61 1.2257118571452992 1103 62-63 1.372334268416843 1103 64-65 1.3398743679594176 1103 66-67 1.4319613013459467 1103 68-69 1.5063061954328845 1103 70-71 1.244663157280737 1103 72-73 1.3493402921044897 1103 74-75 1.304647548006038 1103 76-77 1.0748432605724787 1103 78-79 1.2626301212840225 1103 80-81 1.2953786638280107 1103 82-83 1.4567072700281223 1103 84-85 1.5386005070449187 1103 86-87 1.3167811231851871 1103 88-89 1.5693980142570254 1103 90-91 1.8283757727261367 1103 92-93 1.7321735728730552 1103 94-95 1.5177122852922587 1103 96-97 1.4730514672447796 1103 98-99 1.612994470630337 1103 100-101 2.2737208622456713 1104 1 0.28627280108955233 1104 2 0.2859475094202395 1104 3 0.19395272859640755 1104 4 0.19668717028164195 1104 5 0.09020010950226975 1104 6 0.1815229709321855 1104 7 0.044311392000629723 1104 8 0.12279362095942048 1104 9 0.25040480932090503 1104 10-11 0.19875205860898149 1104 12-13 0.2999085380872444 1104 14-15 0.35633735620694296 1104 16-17 0.12969475889079263 1104 18-19 0.29390046586424745 1104 20-21 0.48403653484831466 1104 22-23 0.3040105466859586 1104 24-25 0.20387580334065802 1104 26-27 0.5345679740773548 1104 28-29 0.39797819277402624 1104 30-31 0.5252305478159727 1104 32-33 0.414947167489359 1104 34-35 0.18771264461786075 1104 36-37 0.061054070897995416 1104 38-39 0.16585887591725879 1104 40-41 0.11144142718373473 1104 42-43 0.3986921076567853 1104 44-45 0.4135275336023483 1104 46-47 0.46476850536546266 1104 48-49 0.4948800081784981 1104 50-51 0.5064980320999055 1104 52-53 0.4322721474744995 1104 54-55 0.4689925184913406 1104 56-57 0.44404753936565555 1104 58-59 0.20974961035289397 1104 60-61 0.5800317735660272 1104 62-63 0.7079791965592861 1104 64-65 0.5907337987409491 1104 66-67 0.4367112736223753 1104 68-69 0.8409229194597927 1104 70-71 0.6748328255511531 1104 72-73 0.8942203803530013 1104 74-75 0.6406776879079459 1104 76-77 0.812944010957203 1104 78-79 0.5977565699623391 1104 80-81 0.6009595651067983 1104 82-83 0.47463518095471 1104 84-85 0.6527992568576622 1104 86-87 0.9383835262839995 1104 88-89 0.7531761245015183 1104 90-91 1.1200063663218955 1104 92-93 0.955762734804015 1104 94-95 0.9924050400038062 1104 96-97 1.2906984459589133 1104 98-99 1.6512627297650084 1104 100-101 2.263879479996934 1105 1 0.008325570096886281 1105 2 -0.09949545262762882 1105 3 -0.11498104341514193 1105 4 0.010459079731518273 1105 5 -0.13922281083883803 1105 6 -0.18867430664239038 1105 7 -0.14734827674456596 1105 8 -0.04917657682864274 1105 9 0.22465330912820036 1105 10-11 -0.07143832739551925 1105 12-13 -0.17084568044699466 1105 14-15 0.09828379084039796 1105 16-17 0.09254281508516726 1105 18-19 0.001270361954688326 1105 20-21 0.05280036584659342 1105 22-23 -0.03430939062627658 1105 24-25 -0.10769335611750819 1105 26-27 -0.20165845739108335 1105 28-29 -0.09297215473977616 1105 30-31 -0.21359043216529017 1105 32-33 0.07157596455304116 1105 34-35 0.23072369920674873 1105 36-37 0.11762620394102896 1105 38-39 -0.12355771438910779 1105 40-41 -0.2933706754342964 1105 42-43 -0.2023255475247865 1105 44-45 0.10510680069663891 1105 46-47 0.2806614869394508 1105 48-49 0.2884098448482888 1105 50-51 0.40778267181118366 1105 52-53 0.5927193848482446 1105 54-55 0.5941957559978235 1105 56-57 0.7050402317489386 1105 58-59 0.6205994940895536 1105 60-61 0.7252448124166122 1105 62-63 0.6987023775038352 1105 64-65 0.9343959790805698 1105 66-67 0.8528727711860675 1105 68-69 0.8186835675736717 1105 70-71 0.7872649627291324 1105 72-73 0.8091733007196318 1105 74-75 1.0431402756159365 1105 76-77 0.934743578574988 1105 78-79 0.9454758431314154 1105 80-81 0.8935233057489391 1105 82-83 0.6909015835296053 1105 84-85 0.8293255481975095 1105 86-87 0.8159866892594216 1105 88-89 0.7565810706068881 1105 90-91 1.0147529479755129 1105 92-93 1.2627043325762521 1105 94-95 1.4145915565193299 1105 96-97 1.3140685258245526 1105 98-99 1.8230363901841677 1105 100-101 1.9049741676173753 1106 1 -0.12270906924378266 1106 2 0.027138317891470365 1106 3 -0.15041125388489718 1106 4 -0.02307152957880021 1106 5 0.12480661405880511 1106 6 -0.05641362990002108 1106 7 -0.03161261646287983 1106 8 -0.10826476827211451 1106 9 0.039283265308718285 1106 10-11 -0.30259367844332274 1106 12-13 -0.4612368015144028 1106 14-15 -0.15445587298317065 1106 16-17 -0.05050055787994978 1106 18-19 -0.1797234094725937 1106 20-21 -0.1777911593590673 1106 22-23 -0.30106145105024495 1106 24-25 -0.062347000551525866 1106 26-27 0.17451176503632126 1106 28-29 0.3311592128973757 1106 30-31 0.06729889903887454 1106 32-33 0.2291293388047393 1106 34-35 -0.4285079083401868 1106 36-37 -0.2364904815198301 1106 38-39 -0.016970033794557082 1106 40-41 -0.13637355889262892 1106 42-43 -0.16926037833918883 1106 44-45 -0.13685962706932742 1106 46-47 -0.06688605985053186 1106 48-49 -0.06561223896120083 1106 50-51 -0.2949808316285356 1106 52-53 -0.3737632500726278 1106 54-55 -0.6377580329875201 1106 56-57 -0.47909431450369766 1106 58-59 -0.14549603087393592 1106 60-61 0.1456223865275419 1106 62-63 -0.1847010871870438 1106 64-65 -0.42025236766096796 1106 66-67 -0.14712155271484662 1106 68-69 -0.16155793152194065 1106 70-71 -0.20495121339643774 1106 72-73 0.06891537877734777 1106 74-75 -0.1181025630390593 1106 76-77 -0.07656888689540864 1106 78-79 0.07510091786672035 1106 80-81 0.16464974653209197 1106 82-83 0.0025515876920785274 1106 84-85 0.22817178645744463 1106 86-87 0.34591675971873315 1106 88-89 0.18641502578840985 1106 90-91 0.1029008746857265 1106 92-93 0.0121196943708064 1106 94-95 -0.4678129147331198 1106 96-97 -0.26778082293767724 1106 98-99 -0.07491113238778624 1106 100-101 0.2285269515547732 1107 1 -0.01621271466632379 1107 2 -0.011153046593072702 1107 3 -0.02890999385758164 1107 4 0.04599912813622353 1107 5 0.05377377640034098 1107 6 0.06314468724212219 1107 7 0.09840104184848997 1107 8 0.043044313543092017 1107 9 0.041322393485231146 1107 10-11 0.04395230774446901 1107 12-13 0.059212191425594085 1107 14-15 -0.1477613767055459 1107 16-17 0.021749023714029647 1107 18-19 0.024396206555728384 1107 20-21 -0.18535613252337413 1107 22-23 -0.008557170804778025 1107 24-25 0.05385925918723444 1107 26-27 -0.28586470419400456 1107 28-29 0.06532230467738032 1107 30-31 0.3015341297873846 1107 32-33 0.1324006005633933 1107 34-35 0.33984424524847867 1107 36-37 0.28722400206134324 1107 38-39 0.04348242181593065 1107 40-41 0.15026341858077075 1107 42-43 0.33750144764238144 1107 44-45 0.2986721814065234 1107 46-47 0.3807939509222251 1107 48-49 0.3992272277875415 1107 50-51 0.2671933406970268 1107 52-53 0.2221046298111773 1107 54-55 0.6604067516536105 1107 56-57 0.4686626445021318 1107 58-59 0.4235698175765208 1107 60-61 0.573170815821733 1107 62-63 0.2881842791827296 1107 64-65 0.5733705475228419 1107 66-67 0.6113283251298292 1107 68-69 0.7172245550602128 1107 70-71 0.7728828862130506 1107 72-73 1.1293582158587014 1107 74-75 1.010434651050165 1107 76-77 1.1676263307719097 1107 78-79 1.1462828427267695 1107 80-81 1.14813473200811 1107 82-83 1.3668629971913404 1107 84-85 1.3224141349175227 1107 86-87 1.6950741765665462 1107 88-89 1.3214158914560308 1107 90-91 1.458478659730833 1107 92-93 1.5184342587476216 1107 94-95 1.381017472124121 1107 96-97 1.038064357217369 1107 98-99 1.3299039032969588 1107 100-101 2.0148753731541404 1108 1 0.020524634401262176 1108 2 0.014399896731667639 1108 3 0.012563608662340187 1108 4 -0.1255445481094668 1108 5 -0.17436527543596014 1108 6 -0.2972670814917535 1108 7 -0.4241731974977938 1108 8 -0.1924673159563426 1108 9 -0.18697643557596422 1108 10-11 -0.15887917293390075 1108 12-13 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52-53 -0.33992045531280013 2316 54-55 -0.6039152382276924 2316 56-57 -0.6897930044600287 2316 58-59 -1.0930942841490392 2316 60-61 -0.3816701898916719 2316 62-63 -0.6792425719032025 2316 64-65 -0.5174139397133715 2316 66-67 -0.43423945664497765 2316 68-69 -0.16264963457870607 2316 70-71 -0.14599924833093425 2316 72-73 -0.26732916270736595 2316 74-75 -0.17487112199102128 2316 76-77 -0.45757325370763624 2316 78-79 -0.15306502099791075 2316 80-81 0.032553676663091835 2316 82-83 0.11718040865277501 2316 84-85 0.04149056375002402 2316 86-87 0.0981001658322711 2316 88-89 0.08488664150893399 2316 90-91 0.427136682545985 2316 92-93 0.21954327515683403 2316 94-95 -0.2178129147331198 2316 96-97 0.39815804169114344 2316 98-99 0.29845131302706207 2316 100-101 0.45996799958970414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 8.0 6 28.0 7 66.0 8 120.0 9 255.0 10 516.0 11 859.0 12 1437.0 13 2086.0 14 2733.0 15 3961.0 16 6095.0 17 9401.0 18 7920.0 19 4158.0 20 3152.0 21 3135.0 22 3010.0 23 3375.0 24 3599.0 25 4051.0 26 4742.0 27 5586.0 28 6655.0 29 8228.0 30 10153.0 31 12628.0 32 16090.0 33 21642.0 34 29810.0 35 43977.0 36 66885.0 37 97786.0 38 74783.0 39 6117.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.081057534069764 20.54705230663325 15.870325155155909 19.501565004141078 2 15.68018200115256 22.477056329184695 40.16217541264203 21.68058625702072 3 18.761772877011605 27.855809672896793 31.44358050282549 21.938836947266115 4 12.42183669783164 18.14850812382271 38.07488194868531 31.35477322966034 5 12.279109773142673 39.58370067734652 33.45597247607784 14.681217073432965 6 29.256298220414063 38.3019533299502 17.432674195573828 15.009074254061911 7 26.263299588003065 36.2370229565726 20.81380146736279 16.68587598806155 8 26.954190069067547 34.1753180289516 19.81778381772361 19.052708084257244 9 28.090837153694643 16.125508545280958 20.786062633855998 34.99759166716841 10-11 24.449631439064877 28.420477710019526 26.643579642706623 20.486311208208974 12-13 26.43513042730264 26.287069095288274 27.914453452613923 19.363347024795168 14-15 23.255033845677474 26.515636961028015 28.193083784178974 22.03624540911554 16-17 20.172690365808556 30.338757816330215 28.452302108151343 21.03624970970988 18-19 20.106568727798184 27.03084816321616 33.49356631086416 19.3690167981215 20-21 21.51157289937469 25.942260220362456 33.779340804899235 18.766826075363614 22-23 21.032367661367925 26.27258505851553 33.70429902321806 18.990748256898488 24-25 20.536735676870542 26.382103506704624 33.86438505801502 19.216775758409813 26-27 20.384865153124636 26.69381093483225 33.748704429239254 19.172619482803853 28-29 20.155513002602994 26.8088977005278 33.78139370357269 19.25419559329651 30-31 20.392988310984578 26.75216964984561 33.55484978023963 19.299992258930185 32-33 19.968412134556996 26.996980982771817 33.372182035557316 19.662424847113872 34-35 20.445563076817216 26.962898772610377 33.29186843621789 19.299669714354522 36-37 20.708759450556066 26.818721347290197 33.008351754212434 19.464167447941307 38-39 20.51007199194929 27.139760714930805 32.878151260504204 19.47201603261571 40-41 20.50738412048545 27.424675090097452 32.59990280656787 19.46803798284923 42-43 20.45563293881848 27.530986459183467 32.29322916162636 19.720151440371698 44-45 20.641132604525943 27.65894996688542 31.8175171808744 19.882400247714234 46-47 20.781009435503986 28.16706518840904 31.08781383587212 19.96411154021486 48-49 20.913360226383286 28.440367958851915 30.19124743039488 20.455024384369917 50-51 21.05087173047315 28.88719971100006 29.78161581930623 20.28031273922056 52-53 21.140969181940942 29.100724220087216 29.307260263368395 20.451046334603443 54-55 20.905081582274672 29.25404040838444 29.298121500391357 20.542756508949537 56-57 21.134410775569183 29.25500804211142 28.985360776859366 20.625220405460034 58-59 21.084846425776043 28.654215012514726 29.483154571961844 20.777783989747384 60-61 21.023455441542023 28.553473590050142 29.764951016230444 20.658119952177394 62-63 21.1006511099834 28.497565863602347 30.130609050170733 20.271173976243517 64-65 21.037002313719753 28.570998511994354 29.941812958550873 20.450186215735012 66-67 21.012703955686675 28.573578868599643 29.59948564891668 20.814231526797002 68-69 20.98485760732133 28.747752939456234 29.544975615630083 20.722413837592356 70-71 20.97829920094957 28.631744407077058 29.481649363942097 20.908307028031274 72-73 20.849263520051608 28.600795613374906 29.15105902591119 21.398881840662295 74-75 20.784019851543484 28.877093314296037 28.95600922047427 21.38287761368621 76-77 20.605330156627648 28.504876873983985 29.583465934992216 21.30632703439615 78-79 20.707469272253427 28.29748071183438 29.180070185699662 21.814979830212536 80-81 20.520608448087525 28.60432811814593 28.94106465513534 21.933998778631207 82-83 20.62289942393415 28.412475190893044 29.107882791349766 21.856742593823043 84-85 20.36303545202569 28.019292507703458 29.145405557670017 22.472266482600833 86-87 20.262250242983583 27.727329416925418 29.091047882817406 22.919372457273596 88-89 20.20892287314107 27.413601059666448 29.318011749223743 23.059464317968743 90-91 19.94834530796046 27.525155318906435 29.414250415581567 23.112248957551543 92-93 19.636037570315324 27.546511327862934 29.51243742581411 23.30501367600764 94-95 19.317517180874397 27.928059657844713 29.16663082838048 23.587792332900406 96-97 19.451373179773444 27.999019464489994 28.94955832896106 23.600049026775498 98-99 19.233374404097614 28.37957557343173 28.600519082853275 23.786530939617375 100-101 19.204685606512708 28.20622002006458 28.642135404737868 23.946958968684847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 351.0 1 266.0 2 209.0 3 260.0 4 251.0 5 275.0 6 488.5 7 949.5 8 1488.5 9 1693.0 10 1556.0 11 1463.0 12 1453.5 13 1519.5 14 1587.0 15 1600.0 16 1648.5 17 1690.0 18 1759.0 19 1827.5 20 1957.0 21 2130.0 22 2346.0 23 2662.5 24 2935.5 25 3260.5 26 3706.5 27 4393.5 28 5114.5 29 5881.0 30 6819.0 31 7819.0 32 8728.5 33 9570.0 34 10626.0 35 11620.5 36 13015.0 37 14088.0 38 14714.0 39 15145.0 40 15109.0 41 15466.0 42 15668.0 43 16129.5 44 17059.5 45 17622.5 46 17172.5 47 16732.5 48 16957.0 49 16217.0 50 14978.0 51 13744.0 52 12398.5 53 11581.0 54 11185.5 55 10511.0 56 9393.5 57 8405.5 58 7857.0 59 7235.5 60 6160.0 61 5048.5 62 4147.5 63 2978.0 64 1920.0 65 1359.0 66 944.5 67 706.5 68 519.5 69 318.0 70 206.5 71 147.0 72 118.0 73 107.0 74 78.5 75 51.5 76 39.5 77 33.0 78 27.0 79 14.0 80 4.5 81 4.5 82 2.5 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04236085427006012 2 0.0 3 0.0 4 0.0 5 4.3005943421380835E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.009031248118489975 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.676337269810687E-4 24-25 0.0 26-27 0.001290178302641425 28-29 0.002257812029622494 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01085900071389866 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.3005943421380835E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.1502971710690418E-4 84-85 2.1502971710690418E-4 86-87 0.0 88-89 0.0 90-91 0.008816218401383072 92-93 8.601188684276167E-4 94-95 0.0 96-97 0.0 98-99 2.1502971710690418E-4 100-101 0.011504089865219374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 465052.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.45481249953781 #Duplication Level Percentage of deduplicated Percentage of total 1 77.16609054155144 43.563971728456444 2 12.639448406439671 14.271153797662672 3 4.2474447369429145 7.1936608867878284 4 1.8705962249980952 4.224166365784429 5 1.0780200729751857 3.0429710545276087 6 0.6485986234397483 2.196990820424959 7 0.4592045815851909 1.8147015986624466 8 0.3053963851190399 1.379287652794563 9 0.24415288947754898 1.2405245037007864 >10 1.2437668385449778 12.806085255003143 >50 0.07085405238555847 2.6934405822684133 >100 0.02412867727626212 2.3308412503328717 >500 0.001148984632202958 0.49189831581700216 >1k 7.65989754801972E-4 1.57234485829505 >5k 3.82994877400986E-4 1.1779613294817703 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5448 1.171481898798414 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4188 0.9005444552437146 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3084 0.6631516475576925 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 950 0.20427823125155897 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.16600294160653 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 553 0.118911433560118 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.1502971710690418E-4 0.0 24-25 0.0 0.0 0.0 2.1502971710690418E-4 0.0 26-27 0.0 0.0 0.0 0.001397693161194877 0.0 28-29 0.0 0.0 0.0 0.004945683493458796 0.0 30-31 0.0 0.0 0.0 0.010966515572452113 0.0 32-33 0.0 0.0 0.0 0.01903012996396102 0.0 34-35 0.0 0.0 0.0 0.02472841746729398 0.0 36-37 0.0 0.0 0.0 0.035479903322639186 0.0 38-39 0.0 0.0 0.0 0.05074701323722938 0.0 40-41 0.0 0.0 0.0 0.0726800443821336 0.0 42-43 0.0 0.0 0.0 0.11052527459294875 0.0 44-45 0.0 0.0 0.0 0.14062943498791533 0.0 46-47 0.0 0.0 0.0 0.20094527063640194 0.0 48-49 0.0 0.0 0.0 0.2817964442685979 0.0 50-51 0.0 0.0 0.0 0.33286600208148764 0.0 52-53 0.0 0.0 0.0 0.42156576038808563 0.0 54-55 0.0 0.0 0.0 0.563162829102982 0.0 56-57 0.0 0.0 0.0 0.7658283374762392 0.0 58-59 0.0 0.0 0.0 0.9859112529351557 0.0 60-61 0.0 0.0 0.0 1.2634071028616156 0.0 62-63 0.0 0.0 0.0 1.54509603227166 0.0 64-65 0.0 0.0 0.0 1.821946793046799 0.0 66-67 0.0 0.0 0.0 2.2023343626089122 0.0 68-69 0.0 0.0 0.0 2.7122773367279356 0.0 70-71 0.0 0.0 0.0 3.0342843380955244 0.0 72-73 0.0 0.0 0.0 3.4686443666514712 0.0 74-75 0.0 0.0 0.0 3.960847389109175 0.0 76-77 0.0 0.0 0.0 4.45778106534323 0.0 78-79 0.0 0.0 0.0 5.056638827485958 0.0 80-81 0.0 0.0 0.0 5.91159698270301 0.0 82-83 0.0 0.0 0.0 6.804400368130876 0.0 84-85 0.0 0.0 0.0 7.881591735977912 0.0 86-87 0.0 0.0 0.0 9.21262568486965 0.0 88-89 0.0 0.0 0.0 10.727402527029236 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1995 0.0 50.235195 1 GTATCAA 3690 0.0 43.89313 1 ATCAACG 5075 0.0 31.820822 3 TATCAAC 5070 0.0 31.75852 2 TCAACGC 5150 0.0 31.44964 4 CAACGCA 5250 0.0 30.850601 5 AACGCAG 5395 0.0 30.021437 6 ACGCAGA 6100 0.0 26.398857 7 CGCAGAG 6120 0.0 26.157349 8 ATCCTAG 170 1.1204429E-7 25.145607 1 GCAGAGT 6345 0.0 24.705723 9 GTACATG 3570 0.0 24.214289 1 AGTACTT 3940 0.0 23.268925 12-13 TACATGG 3605 0.0 23.056921 2 ACTATAC 125 2.25398E-4 22.798685 3 TGGTATC 1005 0.0 22.685259 2 GTATACT 105 0.0021950055 22.617743 4 GATTAGC 105 0.0021950055 22.617743 1 ACATGGG 3675 0.0 22.23001 3 AGAGTAC 5960 0.0 22.196993 10-11 >>END_MODULE