FastQCFastQC Report
Thu 26 May 2016
SRR937338_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937338_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435338
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49371.1340613500314698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.7816914673196458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24240.5568087325250725No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.17825230051132684No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6640.15252516435505287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6370.14632308688880824No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4710.10819179580004502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.045.5448951
GTATCAA35650.044.941981
ATCAACG46850.033.881963
TCAACGC47000.033.78944
CAACGCA47200.033.642355
TATCAAC47500.033.5183682
AACGCAG47900.033.2231526
GTACATG30600.029.2091061
ATATAGG1652.6229827E-928.8036632
CGTCACT1004.9160502E-528.5254881
CGCAGAG55050.028.3903088
ACGCAGA55150.028.3388277
TACATGG30400.027.6714132
ATGGGAG7050.027.6488175
TAGACAG1056.854257E-527.167135
GCAGAGT56500.027.073169
CATGGGA20850.026.4535394
AGTACTT36000.026.06130212-13
ACATGGG30150.026.0092773
GATAGAC950.001221415125.0137063
Produced by FastQC (version 0.11.5)