##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937338_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 435338 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.929098769232183 33.0 31.0 34.0 30.0 34.0 2 32.022738653643835 33.0 31.0 34.0 30.0 34.0 3 32.03393225493754 34.0 31.0 34.0 30.0 34.0 4 35.67532124464209 37.0 35.0 37.0 35.0 37.0 5 35.68522389499653 37.0 35.0 37.0 35.0 37.0 6 35.74888477458894 37.0 35.0 37.0 35.0 37.0 7 35.70231865814608 37.0 35.0 37.0 35.0 37.0 8 35.723669884090064 37.0 35.0 37.0 35.0 37.0 9 37.356603374848966 39.0 37.0 39.0 35.0 39.0 10-11 37.25468601408561 39.0 37.0 39.0 34.0 39.0 12-13 37.131278454901704 39.0 37.0 39.0 34.0 39.0 14-15 38.28616844842398 40.0 38.0 41.0 33.5 41.0 16-17 38.288552802649896 40.0 38.0 41.0 34.0 41.0 18-19 38.3446724154565 40.0 38.0 41.0 34.0 41.0 20-21 38.339895667274625 40.0 38.0 41.0 34.0 41.0 22-23 38.20089792299317 40.0 38.0 41.0 34.0 41.0 24-25 38.053878825188704 40.0 38.0 41.0 33.0 41.0 26-27 37.89165430079616 40.0 38.0 41.0 33.0 41.0 28-29 37.894902351735894 40.0 38.0 41.0 33.0 41.0 30-31 37.6409778149392 40.0 37.0 41.0 33.0 41.0 32-33 37.34550165618439 39.5 36.0 41.0 33.0 41.0 34-35 37.17459537187197 39.0 36.0 41.0 33.0 41.0 36-37 37.12792703600421 40.0 36.0 41.0 33.0 41.0 38-39 36.983161359679144 40.0 36.0 41.0 32.0 41.0 40-41 36.73783473990325 39.5 35.0 41.0 31.0 41.0 42-43 36.39214242726341 39.0 35.0 41.0 30.5 41.0 44-45 35.983553009385815 39.0 35.0 41.0 29.5 41.0 46-47 35.37320771446554 39.0 35.0 41.0 26.0 41.0 48-49 34.86812545654182 39.0 35.0 41.0 23.5 41.0 50-51 33.89913584387304 38.0 34.0 40.0 21.0 40.5 52-53 33.90627053002495 38.0 34.0 40.0 20.0 40.5 54-55 34.180131300277026 39.0 35.0 40.0 18.0 41.0 56-57 33.977152235734074 38.0 34.0 40.0 14.0 41.0 58-59 33.73612342593571 38.0 34.0 40.0 11.0 41.0 60-61 33.44051174030294 38.0 34.0 40.0 7.5 41.0 62-63 33.106629791104844 37.5 33.5 40.0 4.0 41.0 64-65 32.708191106680324 37.0 33.0 40.0 2.0 41.0 66-67 32.33765028552527 36.5 32.5 39.5 2.0 41.0 68-69 31.8546497204471 36.0 32.0 39.0 2.0 41.0 70-71 31.40865258718513 35.0 31.5 39.0 2.0 40.0 72-73 31.001958248533324 35.0 31.0 38.0 2.0 40.0 74-75 30.546559225245673 35.0 31.0 37.0 2.0 39.5 76-77 30.125110833421388 35.0 30.5 37.0 2.0 39.0 78-79 29.65869737996683 35.0 30.0 36.0 2.0 39.0 80-81 29.18208610321176 34.5 29.0 36.0 2.0 37.5 82-83 28.741528421594253 34.0 29.0 35.5 2.0 37.0 84-85 28.335592114632767 34.0 29.0 35.0 2.0 37.0 86-87 27.922565914301074 34.0 27.5 35.0 2.0 36.0 88-89 27.566949129182383 34.0 27.0 35.0 2.0 36.0 90-91 27.184400396932958 34.0 26.0 35.0 2.0 35.5 92-93 26.797883483638 34.0 25.5 35.0 2.0 35.0 94-95 26.33676017255558 33.0 25.0 35.0 2.0 35.0 96-97 25.789015661394135 33.0 23.5 35.0 2.0 35.0 98-99 25.12710813207209 33.0 18.0 35.0 2.0 35.0 100-101 23.394767973390792 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.013506640145312332 1101 2 0.09913885678740186 1101 3 0.08450204149359308 1101 4 -0.02412344137463407 1101 5 -0.0024922283819321933 1101 6 0.015803426892063044 1101 7 0.05009544890536688 1101 8 0.1149963238683469 1101 9 0.2150200974486225 1101 10-11 -0.00715863480516532 1101 12-13 0.12540492089405575 1101 14-15 0.26937597920014866 1101 16-17 0.2903044932556469 1101 18-19 0.13830791257698394 1101 20-21 0.15229844999846875 1101 22-23 0.09937003161536495 1101 24-25 0.14698267883066052 1101 26-27 -0.024319597897580536 1101 28-29 0.18208801239120476 1101 30-31 0.24299810361296892 1101 32-33 0.33645646776438554 1101 34-35 0.2997414789429982 1101 36-37 0.44719489696510806 1101 38-39 0.6406417353318048 1101 40-41 0.7265807500662973 1101 42-43 0.9152639445935264 1101 44-45 1.171382400875082 1101 46-47 1.5493815683912473 1101 48-49 1.7196727240086176 1101 50-51 1.6249039781656478 1101 52-53 1.6278320406330309 1101 54-55 1.6562854970550873 1101 56-57 1.5965788518458197 1101 58-59 1.5747121712730134 1101 60-61 1.658803725572625 1101 62-63 1.651743426096111 1101 64-65 1.5501575257870428 1101 66-67 1.7432469067502439 1101 68-69 1.8505517184143088 1101 70-71 1.8589264644957026 1101 72-73 1.7011623775063178 1101 74-75 1.628473248291563 1101 76-77 1.6056962037490479 1101 78-79 1.674081119394927 1101 80-81 1.4317602748775613 1101 82-83 1.5373967604008598 1101 84-85 1.5552268600768606 1101 86-87 1.7557850518688234 1101 88-89 1.5980531847364041 1101 90-91 1.5856825074540666 1101 92-93 1.5143817608152652 1101 94-95 1.4217498009169596 1101 96-97 1.7728639157663473 1101 98-99 1.7925735563185903 1101 100-101 2.0721609207993374 1102 1 0.06834995087352169 1102 2 -0.04242801911795624 1102 3 0.009089197456894738 1102 4 -0.11298872384686831 1102 5 -0.04534833654224002 1102 6 -0.04628976480277203 1102 7 -0.06616239754562514 1102 8 0.05082442623434957 1102 9 -0.005153731619458313 1102 10-11 0.03052243714075331 1102 12-13 0.1303131777747808 1102 14-15 0.20441581502824846 1102 16-17 0.32595393796834315 1102 18-19 0.3780375118044077 1102 20-21 0.16690250601006085 1102 22-23 0.2905892493845599 1102 24-25 0.005391659999183673 1102 26-27 0.020278422382475014 1102 28-29 0.14268313552013012 1102 30-31 0.1763877704405843 1102 32-33 0.13970248418157638 1102 34-35 0.2381806387691654 1102 36-37 0.2660734580467121 1102 38-39 0.27551257067704427 1102 40-41 0.35943685822660854 1102 42-43 0.32733057906963126 1102 44-45 0.5498626036756633 1102 46-47 0.7090206316457071 1102 48-49 0.6199684748536214 1102 50-51 0.6185761172289048 1102 52-53 0.7306120985760529 1102 54-55 0.6688977616615546 1102 56-57 0.6663507011939558 1102 58-59 0.4989951263671699 1102 60-61 0.7058100027334149 1102 62-63 0.4890140682979407 1102 64-65 0.5296215528271162 1102 66-67 0.811502580337887 1102 68-69 0.9798069091434201 1102 70-71 0.9793755229215861 1102 72-73 0.9664907213015859 1102 74-75 0.9139681782770772 1102 76-77 1.0021520221942453 1102 78-79 0.8630767736682223 1102 80-81 0.9572708977650564 1102 82-83 0.8467750800531988 1102 84-85 0.821966357903996 1102 86-87 0.637315713385 1102 88-89 0.6845850533988873 1102 90-91 0.8039212809934178 1102 92-93 0.8086345372517698 1102 94-95 0.9729931616122727 1102 96-97 0.7808165956311477 1102 98-99 1.048413730147658 1102 100-101 1.3655240304082206 1103 1 -0.18676410813962008 1103 2 -0.29008654085904695 1103 3 -0.24488370789215352 1103 4 -0.2829389075585169 1103 5 -0.3479907412488643 1103 6 -0.3301890727846484 1103 7 -0.28747858445814245 1103 8 -0.22694082859512577 1103 9 -0.44009951977626116 1103 10-11 -0.2643615314817467 1103 12-13 -0.2826294771403468 1103 14-15 -0.44578113964117705 1103 16-17 -0.6304455390733352 1103 18-19 -0.5928391196226812 1103 20-21 -0.5756842216684106 1103 22-23 -0.7591622327063732 1103 24-25 -0.6806107519206606 1103 26-27 -0.5048058257522285 1103 28-29 -0.4289827234163752 1103 30-31 -0.27337329170579494 1103 32-33 -0.16912610044975906 1103 34-35 -0.23005161558693743 1103 36-37 -0.14567591643202604 1103 38-39 -0.29874272089475795 1103 40-41 -0.2525399016859282 1103 42-43 -0.21799998919671992 1103 44-45 -0.015322629514159303 1103 46-47 0.12168856438244546 1103 48-49 0.14662216148437324 1103 50-51 0.19055896329431476 1103 52-53 0.25904373079209364 1103 54-55 0.3108516421475471 1103 56-57 0.13241617241998682 1103 58-59 -0.0016980188213793213 1103 60-61 0.08089735029783185 1103 62-63 -0.09125411690143181 1103 64-65 0.1546525471241651 1103 66-67 0.18200418077067582 1103 68-69 0.2305465457775533 1103 70-71 0.742522740197245 1103 72-73 0.6838030085680025 1103 74-75 0.3960252979488743 1103 76-77 0.577716118400545 1103 78-79 0.801189615450788 1103 80-81 0.6127650595665592 1103 82-83 0.24830197183238312 1103 84-85 0.2787222693617437 1103 86-87 0.11627903867860212 1103 88-89 0.2862026739385257 1103 90-91 0.5051742958932195 1103 92-93 0.46179285612109666 1103 94-95 0.36158556970009315 1103 96-97 0.12399672321494748 1103 98-99 0.358985039572179 1103 100-101 0.28002203550255444 1104 1 0.09839141775664473 1104 2 0.07225559926086689 1104 3 0.13344580006697981 1104 4 0.1408653680976073 1104 5 0.04567902279126912 1104 6 0.07833898389155536 1104 7 0.20112281155066114 1104 8 0.3392111820013284 1104 9 0.3764611486362597 1104 10-11 0.18090359896856967 1104 12-13 0.22685896753328194 1104 14-15 0.4317057748827722 1104 16-17 0.26194769591249667 1104 18-19 0.4340901665658663 1104 20-21 0.3066172717441873 1104 22-23 0.33796941072156983 1104 24-25 0.5066202501251595 1104 26-27 0.22970899209592233 1104 28-29 0.2776211868912384 1104 30-31 0.45369047239501015 1104 32-33 0.5038819244101163 1104 34-35 0.5605832954183398 1104 36-37 0.8221504958388408 1104 38-39 0.8800850968219507 1104 40-41 0.9330185523910401 1104 42-43 0.9885323006689006 1104 44-45 1.1211058865439867 1104 46-47 1.2387938851914768 1104 48-49 1.5015621182761834 1104 50-51 1.455881524913174 1104 52-53 1.6208845278180704 1104 54-55 1.5915768117616054 1104 56-57 1.6236245525172492 1104 58-59 1.6925838050900737 1104 60-61 1.7032301207787413 1104 62-63 1.874239996740826 1104 64-65 1.7395893357646557 1104 66-67 1.8122505887948677 1104 68-69 1.892064966555239 1104 70-71 2.0654963872449628 1104 72-73 2.055123824333254 1104 74-75 1.8833147759790947 1104 76-77 2.0414669062254056 1104 78-79 2.0015094639187865 1104 80-81 1.9943275985365077 1104 82-83 1.716803301444827 1104 84-85 1.7152968911215503 1104 86-87 1.5794728722428033 1104 88-89 1.4051515891837347 1104 90-91 1.705291921936773 1104 92-93 1.828511426543372 1104 94-95 1.9928818491445206 1104 96-97 1.8879509130950645 1104 98-99 1.8352289602605438 1104 100-101 2.038396571654868 1105 1 0.05695557308006016 1105 2 0.06960444383193831 1105 3 0.02693793892949259 1105 4 0.08793458831497247 1105 5 0.1048222115640911 1105 6 0.14907603148046178 1105 7 0.03409342461386444 1105 8 0.2266359999817027 1105 9 0.25177110959437243 1105 10-11 0.17094527882506583 1105 12-13 0.04649466448378803 1105 14-15 0.1414306350705985 1105 16-17 -0.028911093788892117 1105 18-19 -0.036938376221932856 1105 20-21 -0.039012278746469065 1105 22-23 0.16516287911873917 1105 24-25 0.025570735510818565 1105 26-27 0.24472561127920756 1105 28-29 0.2299861230524698 1105 30-31 0.19119311036060793 1105 32-33 0.4775715149978552 1105 34-35 0.35494728191196145 1105 36-37 0.41675123974513895 1105 38-39 0.6905472683952283 1105 40-41 0.4111893870433576 1105 42-43 0.40792926173253363 1105 44-45 0.6364819285403982 1105 46-47 1.277519220213101 1105 48-49 1.3166868940490986 1105 50-51 1.2749731414584957 1105 52-53 1.2672652390136037 1105 54-55 1.386280436326338 1105 56-57 1.3165653565691642 1105 58-59 1.5662219553485883 1105 60-61 1.7679130373135123 1105 62-63 1.8595959901986774 1105 64-65 1.6467263516979713 1105 66-67 1.5675232225397195 1105 68-69 1.5385577912888024 1105 70-71 1.5644510933755953 1105 72-73 1.5418185853335764 1105 74-75 1.664350441274017 1105 76-77 1.6230426949771228 1105 78-79 1.5916553434165195 1105 80-81 1.4975261318276338 1105 82-83 1.39761605864647 1105 84-85 1.5027032433427188 1105 86-87 1.4079358615854218 1105 88-89 1.4519853709712187 1105 90-91 1.5753721160910423 1105 92-93 1.6638891832174245 1105 94-95 1.7487150505795732 1105 96-97 2.058155751123973 1105 98-99 2.0662257155628545 1105 100-101 2.239627182607702 1106 1 -0.04197646335197547 1106 2 -0.16951792029042778 1106 3 -0.022524985533433295 1106 4 0.1731860485664356 1106 5 0.13358174032362058 1106 6 0.24457400197842816 1106 7 0.2734199764404224 1106 8 0.15687805522375697 1106 9 0.11992154024480328 1106 10-11 -0.3828241499443621 1106 12-13 -0.2745564690673348 1106 14-15 -0.257644951504993 1106 16-17 -0.2621584207862213 1106 18-19 -0.017812794596352433 1106 20-21 0.04269064670645406 1106 22-23 0.2402235166793787 1106 24-25 0.2502421789322682 1106 26-27 0.03538371443730881 1106 28-29 0.039602745169091236 1106 30-31 0.13510336677086343 1106 32-33 0.14080720948354752 1106 34-35 -0.15660479214011502 1106 36-37 0.07475230399619903 1106 38-39 0.17861775646571942 1106 40-41 0.3610087118626808 1106 42-43 0.2608522396555131 1106 44-45 0.33629197210044026 1106 46-47 0.2986804913743768 1106 48-49 0.6949724598346734 1106 50-51 0.7611100338453909 1106 52-53 0.4080433797917422 1106 54-55 0.48749256878847547 1106 56-57 0.28755832756213806 1106 58-59 0.6264171092937403 1106 60-61 0.6497811691816437 1106 62-63 0.7268399511926376 1106 64-65 0.7511776436492639 1106 66-67 0.8367075854740804 1106 68-69 0.6284931937242035 1106 70-71 0.8444883421628475 1106 72-73 0.8076183823833709 1106 74-75 0.8466219985455794 1106 76-77 1.069994110678536 1106 78-79 1.2031727674339407 1106 80-81 1.1564646032661017 1106 82-83 1.030418503948919 1106 84-85 0.9722112128506843 1106 86-87 1.3030112254107884 1106 88-89 1.1663360540719054 1106 90-91 1.2228822636011891 1106 92-93 1.5071596514878927 1106 94-95 1.4792584373729625 1106 96-97 1.5885456877639115 1106 98-99 1.6248678054549437 1106 100-101 1.6026791081596272 1107 1 -0.2595377014050193 1107 2 -0.05917865831182212 1107 3 -0.07336681096542108 1107 4 -0.09738487364383275 1107 5 -0.04608152950358857 1107 6 0.05786123534763021 1107 7 -0.20148349846305535 1107 8 -0.15892516016962333 1107 9 -0.20369862558847984 1107 10-11 -0.1981961671606456 1107 12-13 -0.17557868566332502 1107 14-15 -0.13323834349182562 1107 16-17 -0.16191077853037683 1107 18-19 -0.2592481702530378 1107 20-21 -0.19597161039842348 1107 22-23 -0.12048760427764194 1107 24-25 -0.14486589752826973 1107 26-27 0.006968150161092979 1107 28-29 -0.08742665542588668 1107 30-31 -0.013462206999633963 1107 32-33 0.03729986724670198 1107 34-35 0.06858746686361883 1107 36-37 0.09454390473042196 1107 38-39 0.1969575248054909 1107 40-41 0.18701431348303998 1107 42-43 0.014539181867043283 1107 44-45 -0.37524831525951896 1107 46-47 -0.24115984662169865 1107 48-49 0.014905683456419183 1107 50-51 -0.04602570259363148 1107 52-53 -0.13996994383634132 1107 54-55 0.3157807515848461 1107 56-57 0.06674925736782455 1107 58-59 -0.2295824792878136 1107 60-61 -0.29372000177853863 1107 62-63 -0.09333854111700646 1107 64-65 -0.2299287554899223 1107 66-67 -0.05491530205044626 1107 68-69 -0.0784819312837115 1107 70-71 0.07920256458198338 1107 72-73 -0.04076600903380623 1107 74-75 -0.0627670450647777 1107 76-77 0.08018067311919452 1107 78-79 0.09832831525342556 1107 80-81 0.14257131888101782 1107 82-83 0.14181004771751304 1107 84-85 0.4161910533469211 1107 86-87 0.43792482753750406 1107 88-89 0.1892568085500379 1107 90-91 0.1134133098699408 1107 92-93 0.3596384476142269 1107 94-95 0.48538224267709396 1107 96-97 0.5983643471727369 1107 98-99 0.5719135045498227 1107 100-101 0.8615008690305679 1108 1 -0.2831646561530725 1108 2 -0.1912516355725309 1108 3 -0.2673873594280636 1108 4 -0.218768117581277 1108 5 -0.26478667417382695 1108 6 -0.1478783582158698 1108 7 -0.024064143624343615 1108 8 0.0036211116442359526 1108 9 -0.07499688048007158 1108 10-11 -0.09440093410179173 1108 12-13 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-3.057503184105176 2316 94-95 -2.586634532008379 2316 96-97 -2.330085900635119 2316 98-99 -2.092812000596954 2316 100-101 -2.049736264023185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1624.0 3 261.0 4 199.0 5 225.0 6 251.0 7 308.0 8 384.0 9 486.0 10 675.0 11 1035.0 12 1666.0 13 2534.0 14 2899.0 15 4044.0 16 5680.0 17 8351.0 18 6593.0 19 3649.0 20 3334.0 21 3220.0 22 3458.0 23 3554.0 24 3996.0 25 4229.0 26 4673.0 27 5284.0 28 6109.0 29 7290.0 30 8830.0 31 11031.0 32 13909.0 33 18582.0 34 25809.0 35 37732.0 36 56643.0 37 85165.0 38 79620.0 39 12005.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45060518731989 19.808184438040346 15.715734870317002 21.025475504322767 2 14.745659212148446 21.39642597905361 41.21438364845091 22.643531160347035 3 17.86775854044065 27.605220348668414 31.449743544841635 23.0772775660493 4 11.4680812331062 17.164215217496505 38.8829871808757 32.484716368521596 5 11.819616789372416 38.587904554514765 34.42051778369223 15.171960872420588 6 28.454071577241162 37.91190068335452 18.23944115967656 15.394586579727761 7 25.492881874102174 35.6389738111901 21.797533979152085 17.07061033555564 8 25.79707208814585 33.831908667935934 20.69083801635008 19.680181227568138 9 27.082139149211436 15.824464717758179 21.183617750582993 35.90977838244739 10-11 23.554104370211913 27.84740755409516 27.557848489802684 21.04063958589024 12-13 25.38242282472556 25.8326502983865 28.57557282840934 20.2093540484786 14-15 22.45715496044719 26.07745720100563 28.46927405169953 22.996113786847655 16-17 19.600006446310292 29.910304196778227 28.80338062929802 21.68630872761346 18-19 19.458432850853214 27.047624528790536 33.560818393715294 19.93312422664096 20-21 20.799642730944402 25.962433803361595 33.69010362414006 19.547819841553938 22-23 20.40036233343654 26.250528019263065 33.60769233425375 19.74141731304665 24-25 19.799423174748586 26.44948635852014 33.74347158571702 20.007618881014256 26-27 19.449268657042794 26.656469643138703 33.72533177730897 20.168929922509527 28-29 19.490064182251814 26.76029264160152 33.783276701934675 19.966366474211995 30-31 19.572272422197074 26.67454874706005 33.608515409774164 20.144663420968715 32-33 19.25283089238292 26.965336317036925 33.3296497486529 20.452183041927253 34-35 19.647924699034448 27.018342867602783 33.2017070736259 20.132025359736872 36-37 19.947608667128584 26.873577135648897 32.88948789543832 20.289326301784204 38-39 19.651750168899675 27.153772936690533 32.85136023221259 20.343116662197204 40-41 19.580589398379075 27.394042486586272 32.767129404778075 20.25823871025658 42-43 19.527227902255092 27.380290573677563 32.30068379078603 20.79179773328131 44-45 19.564289060494687 27.65810261893882 31.97055204555317 20.807056275013323 46-47 19.891659309276296 27.91856260956948 31.257187124816255 20.932590956337965 48-49 19.878712250108784 28.3329380967401 30.45616442456238 21.332185228588727 50-51 20.176458534316627 28.534754088547004 30.068865798035105 21.219921579101268 52-53 20.164105823615024 28.774710293115202 29.804229075701468 21.256954807568302 54-55 20.0292168771786 28.815461691125343 29.650295392067928 21.505026039628124 56-57 20.219143336221947 28.873820530791356 29.43382025458927 21.47321587839743 58-59 20.286859540559792 28.179467320039542 29.983646497346726 21.550026642053936 60-61 20.177593851883138 28.25367196463774 30.228356324709505 21.340377858769617 62-63 20.189046446776164 28.07557254280476 30.58084653125205 21.154534479167026 64-65 20.20187605946954 28.075640802854945 30.575787531834436 21.146695605841078 66-67 20.19390661411433 28.056158117322134 30.230789148134296 21.51914612042924 68-69 20.214959723820485 28.119792865362488 30.234982738780207 21.430264672036824 70-71 20.066843827361545 27.964497272960593 30.22024626794385 21.748412631734016 72-73 19.932512527304574 28.051840138473615 30.031925349464032 21.983721984757775 74-75 19.831686917409886 28.113026654558805 30.039235455316582 22.016050972714726 76-77 19.740578117177204 27.98096345189934 30.2500417215761 22.028416709347358 78-79 19.781743771054234 27.62027743538573 30.10713706155116 22.49084173200888 80-81 19.651704933240186 27.93024430835312 29.803961738413854 22.614089019992843 82-83 19.839019292826528 27.522775294502168 30.048312625730212 22.58989278694109 84-85 19.63232755644549 27.13986512923197 29.974107574397568 23.25369973992497 86-87 19.697253903719194 27.048639840548503 30.010506583012937 23.243599672719366 88-89 19.546272119349258 26.49684284660504 30.209245562896225 23.747639471149476 90-91 19.058711151263356 26.500405678345984 30.627046373926103 23.813836796464557 92-93 18.968767262014364 26.625391272325537 30.63570244890444 23.77013901675566 94-95 18.64737779700885 26.85554292237653 30.287330660418615 24.209748620196002 96-97 18.62996578712063 27.06523002493754 29.991564735762182 24.313239452179648 98-99 18.786461456306096 27.292187681623943 29.75554064520881 24.165810216861146 100-101 18.851868558777092 27.18616083552356 29.829622605901875 24.13234799979747 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 785.0 1 479.5 2 206.5 3 249.5 4 240.5 5 265.0 6 433.0 7 884.5 8 1507.5 9 1737.5 10 1501.5 11 1348.0 12 1382.0 13 1388.5 14 1426.5 15 1495.0 16 1541.5 17 1540.0 18 1591.5 19 1729.5 20 1858.5 21 1986.5 22 2230.5 23 2577.0 24 2804.0 25 3095.0 26 3609.5 27 4264.5 28 4896.0 29 5579.5 30 6431.5 31 7384.5 32 8362.0 33 9157.0 34 10065.0 35 10940.5 36 12117.0 37 13180.5 38 13786.0 39 14146.5 40 14092.0 41 14296.0 42 14603.5 43 15251.0 44 15935.5 45 16207.0 46 15979.5 47 15706.5 48 15833.0 49 15190.0 50 13962.0 51 12829.0 52 11696.0 53 10965.0 54 10420.5 55 9668.5 56 8798.0 57 7776.0 58 7098.0 59 6431.5 60 5491.5 61 4749.0 62 3882.0 63 2698.0 64 1730.0 65 1239.5 66 868.0 67 597.5 68 436.5 69 289.5 70 197.5 71 144.0 72 109.5 73 87.0 74 66.0 75 57.5 76 46.0 77 30.5 78 17.0 79 10.0 80 8.5 81 4.0 82 3.5 83 2.5 84 2.0 85 1.5 86 2.0 87 3.0 88 2.0 89 1.5 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.5 96 2.0 97 3.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36477403764431315 2 0.3153871244871801 3 0.3087256338752877 4 0.31630595077847556 5 0.3153871244871801 6 0.2320036385521135 7 0.21914007047397652 8 0.2221262559206869 9 0.21615388502726618 10-11 0.24279984747483563 12-13 0.23016598596952254 14-15 0.225571854513045 16-17 0.22534214794022117 18-19 0.21833609746909297 20-21 0.21397167258543937 22-23 0.21397167258543937 24-25 0.20466855638607245 26-27 0.2061616491094276 28-29 0.21822124418268107 30-31 0.23418585099394032 32-33 0.22476788150816146 34-35 0.23602350357653135 36-37 0.21018151413384542 38-39 0.20868842141049024 40-41 0.20558738267736792 42-43 0.21167460685720063 44-45 0.2169578580321497 46-47 0.22224110920709886 48-49 0.2294768662510509 50-51 0.2331521714162329 52-53 0.2540554695432055 54-55 0.2297065728238748 56-57 0.2006486913616546 58-59 0.2008783979344785 60-61 0.19835162563341585 62-63 0.20547252939095598 64-65 0.19835162563341585 66-67 0.1953654401867055 68-69 0.19249410802640707 70-71 0.20501311624530824 72-73 0.20374973009477693 74-75 0.20891812798331413 76-77 0.20914783455613797 78-79 0.20547252939095598 80-81 0.20845871483766634 82-83 0.2012229577937143 84-85 0.19479117375464583 86-87 0.19490602704105775 88-89 0.19490602704105775 90-91 0.20225663737142174 92-93 0.19708823948288456 94-95 0.19662882633723683 96-97 0.19582485333235325 98-99 0.2043239965268366 100-101 0.19111586858946383 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 435338.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.24417931798551 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7117760900007 46.632145771131256 2 11.999699366034138 14.21824682006487 3 3.903992895009581 6.93866565384267 4 1.7693658659104112 4.192985145564764 5 0.9755879511156272 2.889895375818015 6 0.565215497070674 2.0091436957055597 7 0.3770743410863561 1.5637621915671918 8 0.2732165865569371 1.2949193957301672 9 0.21213136871230281 1.1310793962265233 >10 1.1279065509225965 11.858947880221772 >50 0.06350917956350878 2.5027835037772768 >100 0.01820080144916847 1.82139501575591 >500 0.0011617532839894766 0.4765130933020564 >1k 0.0011617532839894766 2.469517061291928 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4937 1.1340613500314698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3403 0.7816914673196458 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2424 0.5568087325250725 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.17825230051132684 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 664 0.15252516435505287 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 637 0.14632308688880824 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 471 0.10819179580004502 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.1485328641193739E-4 0.0 20-21 0.0 0.0 0.0 3.4455985923581214E-4 0.0 22-23 0.0 0.0 0.0 8.039730048835618E-4 0.0 24-25 0.0 0.0 0.0 0.0013782394369432488 0.0 26-27 0.0 0.0 0.0 0.00264162558747456 0.0 28-29 0.0 0.0 0.0 0.005972370893420745 0.0 30-31 0.0 0.0 0.0 0.013437834510196676 0.0 32-33 0.0 0.0 0.0 0.019754765262853233 0.0 34-35 0.0 0.0 0.0 0.024693456578566537 0.0 36-37 0.0 0.0 0.0 0.03537481221487672 0.0 38-39 0.0 0.0 0.0 0.052717658463079256 0.0 40-41 0.0 0.0 0.0 0.07580316903187867 0.0 42-43 0.0 0.0 0.0 0.10750267608157339 0.0 44-45 0.0 0.0 0.0 0.1305881866503728 0.0 46-47 0.0 0.0 0.0 0.18698115027863407 0.0 48-49 0.0 0.0 0.0 0.25417032282961743 0.0 50-51 0.0 0.0 0.0 0.2947135329330314 0.0 52-53 0.0 0.0 0.0 0.3751108334213875 0.0 54-55 0.0 0.0 0.0 0.5021385681929903 0.0 56-57 0.0 0.0 0.0 0.6893494250444482 0.0 58-59 0.0 0.0 0.0 0.8803504403475001 0.0 60-61 0.0 0.0 0.0 1.1440535859493084 0.0 62-63 0.0 0.0 0.0 1.3992575883566332 0.0 64-65 0.0 0.0 0.0 1.6505565790259522 0.0 66-67 0.0 0.0 0.0 2.0168237093936208 0.0 68-69 0.0 0.0 0.0 2.487951890255388 0.0 70-71 0.0 0.0 0.0 2.8056360804708067 0.0 72-73 0.0 0.0 0.0 3.2268030817433813 0.0 74-75 0.0 0.0 0.0 3.718375147586473 0.0 76-77 0.0 0.0 0.0 4.1951311394824256 0.0 78-79 0.0 0.0 0.0 4.784902765207724 0.0 80-81 0.0 0.0 0.0 5.642397401559249 0.0 82-83 0.0 0.0 0.0 6.5376787691403 0.0 84-85 0.0 0.0 0.0 7.643601064000846 0.0 86-87 0.0 0.0 0.0 9.022989033808212 0.0 88-89 0.0 0.0 0.0 10.599350389812054 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1785 0.0 45.544895 1 GTATCAA 3565 0.0 44.94198 1 ATCAACG 4685 0.0 33.88196 3 TCAACGC 4700 0.0 33.7894 4 CAACGCA 4720 0.0 33.64235 5 TATCAAC 4750 0.0 33.518368 2 AACGCAG 4790 0.0 33.223152 6 GTACATG 3060 0.0 29.209106 1 ATATAGG 165 2.6229827E-9 28.803663 2 CGTCACT 100 4.9160502E-5 28.525488 1 CGCAGAG 5505 0.0 28.390308 8 ACGCAGA 5515 0.0 28.338827 7 TACATGG 3040 0.0 27.671413 2 ATGGGAG 705 0.0 27.648817 5 TAGACAG 105 6.854257E-5 27.16713 5 GCAGAGT 5650 0.0 27.07316 9 CATGGGA 2085 0.0 26.453539 4 AGTACTT 3600 0.0 26.061302 12-13 ACATGGG 3015 0.0 26.009277 3 GATAGAC 95 0.0012214151 25.013706 3 >>END_MODULE