FastQCFastQC Report
Thu 26 May 2016
SRR937338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937338_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435338
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52701.21055363878182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40800.9372028171214091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30020.6895791316172721No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.21362711272620355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.17549582163744032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5480.1258792019074834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.049.33411
GTATCAA41450.040.018551
ACGCAGT600.00394306931.6752098
CTATACC600.003949728331.6642914
TATCAAC53350.031.0707722
ATCAACG53450.031.0126423
TCAACGC54150.030.6117424
CAACGCA55450.029.8940625
AACGCAG56350.029.4166076
ATACCTA650.005842275529.228585
GATAGTC1004.9370487E-528.5076877
ACGCAGA63500.026.113347
CGCAGAG63550.026.0180328
GTACATG34050.025.404741
TACATGG34850.024.940742
ACATGGG33800.024.8723953
CATGGGA24150.024.7807524
GCAGAGT66050.024.3858349
GAGTACT39750.024.38393412-13
TGGTACA1201.709307E-423.7482194
Produced by FastQC (version 0.11.5)