FastQCFastQC Report
Thu 26 May 2016
SRR937337_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937337_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513123
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55471.081027356014055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40200.7834378891610783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.5367134195894552No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8860.17266815169072525No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.16078016382037055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.14265585444425605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.10289930484503715No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.049.3967061
GTATCAA38650.044.790071
TATCAAC51550.033.3841132
ATCAACG52300.032.9053763
TCAACGC53550.032.226054
CAACGCA54400.031.635135
AACGCAG56000.030.8769046
TAAGGGT1004.9194387E-528.5240523
GTATAGA856.3562195E-427.9756971
GTACATG37850.027.6430931
CGTCACT700.00833692827.1763921
GCGTAGG700.00833692827.1763921
ACGCAGA63850.026.7805547
CGCAGAG63850.026.7035548
TACATGG38300.026.6869932
GTATAGG1109.38741E-525.9411011
CAGTCTA1658.401912E-825.9056289
GCAGAGT66250.025.4494299
ACATGGG37850.025.245863
CCGTATC851.1261545E-725.13878692-93
Produced by FastQC (version 0.11.5)