FastQCFastQC Report
Thu 26 May 2016
SRR937337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937337_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513123
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63501.2375200487992157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.9703326492868182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34250.6674812861633566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.19839297790198454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.1703295311260653No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6420.12511620020930653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.046.510561
GTATCAA47300.041.705121
TAGACCC702.0514075E-433.9295464
TATCAAC62200.031.6166632
ATCAACG62550.031.439753
TCAACGC63400.031.0182424
CAACGCA64350.030.560325
AACGCAG66900.029.6055876
ATAAGCG650.00584027729.2316073
TCAAACG650.00584027729.2316073
ACGCAGA76250.025.7261037
CGCAGAG76200.025.6183198
GCAGAGT78500.024.565199
AGTACTT46750.024.0785612-13
GAGTACT45850.023.30810512-13
GTACATG40350.022.9717221
ATGGGAG10250.022.7079685
GTACTTT47400.022.39561314-15
TAGCACA1503.0283192E-522.1673034
GAGTACC2154.4126864E-822.0915036
Produced by FastQC (version 0.11.5)