FastQCFastQC Report
Thu 26 May 2016
SRR937336_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937336_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695314
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72901.0484471763836194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50680.7288793264625767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34910.5020753213655988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11070.1592086453026978No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.1535996686389171No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.12728062429348466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.051.0432241
GTATCAA53400.043.5235371
TATCAAC68350.033.9244272
ATCAACG69100.033.5610583
TCAACGC70550.032.876034
CAACGCA71400.032.420425
AACGCAG73000.031.7006846
GGGCGCG804.483493E-429.6969057
GTCGGGA2101.8189894E-1229.4140782
GTACATG53550.027.8693451
CGCAGAG82350.027.5780878
ACGCAGA83400.027.4607357
TACATGG55250.026.5740282
ACATGGG53450.026.2281843
GCAGAGT85250.025.971169
GAGTACT50000.023.89317112-13
AGTACTT51700.023.29127312-13
CATGGGA37400.023.2560564
ATGGGAT13100.022.8595
TGGTATC13150.022.4025332
Produced by FastQC (version 0.11.5)