##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937336_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 695314 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.877876757838905 33.0 31.0 34.0 30.0 34.0 2 31.957400541338156 33.0 31.0 34.0 30.0 34.0 3 31.976127332399464 33.0 31.0 34.0 30.0 34.0 4 35.62436827102575 37.0 35.0 37.0 33.0 37.0 5 35.649303767794116 37.0 35.0 37.0 33.0 37.0 6 35.69680029454319 37.0 35.0 37.0 35.0 37.0 7 35.580333489617644 37.0 35.0 37.0 33.0 37.0 8 35.6058787828233 37.0 35.0 37.0 33.0 37.0 9 37.24453124775281 39.0 37.0 39.0 34.0 39.0 10-11 37.139988551934806 39.0 37.0 39.0 34.0 39.0 12-13 37.00609077337721 39.0 37.0 39.0 33.0 39.0 14-15 38.143154028251985 40.0 38.0 41.0 33.0 41.0 16-17 38.110288301400516 40.0 38.0 41.0 33.0 41.0 18-19 38.16384180384689 40.0 38.0 41.0 33.5 41.0 20-21 38.10001812130922 40.0 38.0 41.0 33.5 41.0 22-23 37.959286739516244 40.0 38.0 41.0 33.0 41.0 24-25 37.892178929231974 40.0 38.0 41.0 33.0 41.0 26-27 37.86788343108293 40.0 38.0 41.0 33.0 41.0 28-29 37.76385345325997 40.0 37.5 41.0 33.0 41.0 30-31 37.483756259761776 39.5 37.0 41.0 33.0 41.0 32-33 37.179648331545174 39.0 36.0 41.0 32.0 41.0 34-35 37.156142404726495 39.5 36.0 41.0 32.0 41.0 36-37 37.01436473305586 39.5 36.0 41.0 31.5 41.0 38-39 36.862712386058675 39.0 35.0 41.0 31.0 41.0 40-41 36.59825057456056 39.0 35.0 41.0 30.5 41.0 42-43 36.24514320148882 39.0 35.0 41.0 30.0 41.0 44-45 35.85854664223646 39.0 35.0 41.0 29.5 41.0 46-47 35.29928852288319 39.0 35.0 41.0 26.0 41.0 48-49 34.81587455451781 39.0 35.0 40.5 23.5 41.0 50-51 33.76545848350529 38.0 34.0 39.5 21.5 40.5 52-53 33.83977095240424 38.0 33.5 39.5 21.0 40.5 54-55 34.181502889342084 38.0 34.0 40.0 20.0 41.0 56-57 33.97052914222927 38.0 34.0 40.0 18.5 41.0 58-59 33.71694802635932 38.0 34.0 40.0 16.5 41.0 60-61 33.413065176308834 38.0 33.5 40.0 13.0 41.0 62-63 33.10165335373658 37.0 33.0 40.0 8.5 41.0 64-65 32.67307144685711 37.0 33.0 40.0 7.0 41.0 66-67 32.322680831969436 36.0 32.0 39.0 2.0 41.0 68-69 31.849124280540877 36.0 31.5 39.0 2.0 40.5 70-71 31.37420935002028 35.0 31.0 38.5 2.0 40.0 72-73 30.932877088624707 35.0 31.0 38.0 2.0 40.0 74-75 30.45624351012636 35.0 30.5 37.0 2.0 39.0 76-77 29.99750400538462 35.0 30.0 36.5 2.0 39.0 78-79 29.507496613041013 34.0 29.0 36.0 2.0 39.0 80-81 29.02199366041817 34.0 29.0 36.0 2.0 37.5 82-83 28.559935511150357 34.0 29.0 35.0 2.0 37.0 84-85 28.062086337971046 34.0 27.5 35.0 2.0 36.5 86-87 27.625530335934556 34.0 26.5 35.0 2.0 36.0 88-89 27.19878932396011 33.5 26.0 35.0 2.0 36.0 90-91 26.727548273154287 33.0 25.0 35.0 2.0 35.0 92-93 26.264177767167062 33.0 24.5 35.0 2.0 35.0 94-95 25.780337372755334 33.0 23.0 35.0 2.0 35.0 96-97 25.12529519037442 32.0 19.0 35.0 2.0 35.0 98-99 24.29623306880057 32.0 3.5 34.0 2.0 35.0 100-101 21.917121616996063 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11775179426170368 1101 2 0.036190902101754574 1101 3 -0.07829201378283202 1101 4 -0.026132259993183027 1101 5 -0.08444658802883964 1101 6 -0.16448437477069433 1101 7 -0.1148307712782497 1101 8 -0.03515981841648852 1101 9 -0.21217591727464225 1101 10-11 -0.13664919143088383 1101 12-13 -0.1378114296827775 1101 14-15 -0.12166384905876271 1101 16-17 -0.16709554045687725 1101 18-19 -0.19734334041917378 1101 20-21 -0.09313092628506325 1101 22-23 -0.16210440758936784 1101 24-25 -0.06302369832949495 1101 26-27 -0.1466136835608367 1101 28-29 -0.09636609534280893 1101 30-31 -0.1885612190651358 1101 32-33 -0.10857831206043045 1101 34-35 -0.0680110082149028 1101 36-37 0.11969747846473666 1101 38-39 -0.01264866224904182 1101 40-41 0.08363540462136854 1101 42-43 0.2409318799637532 1101 44-45 0.31581150285469306 1101 46-47 0.42491506753277974 1101 48-49 0.2502351934166782 1101 50-51 0.39403959456096516 1101 52-53 0.3124743546563593 1101 54-55 0.541206361806239 1101 56-57 0.5790343151023265 1101 58-59 0.5210155681455149 1101 60-61 0.49968349159915704 1101 62-63 0.5853088843899457 1101 64-65 0.30964663228009215 1101 66-67 0.4568304480999714 1101 68-69 0.575498887073639 1101 70-71 0.43414604997676776 1101 72-73 0.4804940451057469 1101 74-75 0.4803018098515146 1101 76-77 0.5276076667499652 1101 78-79 0.28408531145386107 1101 80-81 0.5632802950100917 1101 82-83 0.476968576055814 1101 84-85 0.23846506002169932 1101 86-87 0.2500488310908402 1101 88-89 0.16798869814565265 1101 90-91 0.24243708931938457 1101 92-93 0.12334721067400523 1101 94-95 0.1441177765195043 1101 96-97 0.36826498958948406 1101 98-99 0.5688258600426046 1101 100-101 0.626051317173328 1102 1 0.029625435805499478 1102 2 0.14973728053093893 1102 3 0.20743024433852852 1102 4 0.07754364907102484 1102 5 0.20682110861687875 1102 6 0.05923353629081163 1102 7 0.03684196560713815 1102 8 0.07623359050086265 1102 9 0.03924128298383067 1102 10-11 -0.0439562172195167 1102 12-13 0.03311173309698745 1102 14-15 0.18760375947087482 1102 16-17 0.10896552398534709 1102 18-19 0.10098318839478537 1102 20-21 0.018102405090132834 1102 22-23 0.07452471183729159 1102 24-25 0.11255786778693988 1102 26-27 -0.06447114111825414 1102 28-29 -0.053063651590242955 1102 30-31 -0.048406722207943176 1102 32-33 -0.15408145486721736 1102 34-35 -0.029894195667168333 1102 36-37 0.12887917381808478 1102 38-39 0.021650521208627538 1102 40-41 -0.09261951519300027 1102 42-43 -0.26350917037989774 1102 44-45 -0.024464153610090023 1102 46-47 0.3488283025677248 1102 48-49 0.21238860350890576 1102 50-51 0.3314463383781501 1102 52-53 0.2484705069209454 1102 54-55 0.19190468172634922 1102 56-57 0.25097301568390407 1102 58-59 0.32781005207902325 1102 60-61 0.27960844607252255 1102 62-63 0.29770020789343477 1102 64-65 0.37089464966832963 1102 66-67 0.2621166784941451 1102 68-69 0.12617958377809302 1102 70-71 0.1411255776745861 1102 72-73 0.2478617241282457 1102 74-75 0.38684736586396795 1102 76-77 0.1639783123470977 1102 78-79 0.2462115524219648 1102 80-81 0.2342431100250728 1102 82-83 0.29847403925266747 1102 84-85 0.09222174215763346 1102 86-87 0.14694641083589133 1102 88-89 -0.021213410765824392 1102 90-91 -0.07874675019186483 1102 92-93 0.12314454369403904 1102 94-95 0.02073397225468554 1102 96-97 -0.2734102439181214 1102 98-99 -0.2916687648766221 1102 100-101 2.1201152802419188E-4 1103 1 -0.15796108861185232 1103 2 -0.20763872759759394 1103 3 -0.27609995188932146 1103 4 -0.043677853437394276 1103 5 -0.1678058044459263 1103 6 -0.11363431155322701 1103 7 -0.005599451805700539 1103 8 -0.16163711503035216 1103 9 -0.1292590662890447 1103 10-11 -0.09599549454217993 1103 12-13 -0.08796688505249506 1103 14-15 -0.18467111828362448 1103 16-17 -0.10709070894791495 1103 18-19 -0.14510892438860878 1103 20-21 -0.17293239518928516 1103 22-23 -0.18492073808968001 1103 24-25 -0.2776799552904734 1103 26-27 -0.19251537176536004 1103 28-29 -0.29602977212113757 1103 30-31 -0.3867346576877182 1103 32-33 -0.21479778178662912 1103 34-35 -0.07952349013595494 1103 36-37 -0.05181873689506489 1103 38-39 -0.1862700350878086 1103 40-41 -0.025750429017854515 1103 42-43 0.03109615126867027 1103 44-45 -0.22308214433848406 1103 46-47 0.21971005782269515 1103 48-49 0.3492630875376008 1103 50-51 0.27219536993972326 1103 52-53 0.4362896153225435 1103 54-55 0.6788204434587399 1103 56-57 0.6237924416062981 1103 58-59 0.6005723242578469 1103 60-61 0.62081867659262 1103 62-63 0.6438399487964688 1103 64-65 0.3472155361863969 1103 66-67 0.5700650178603723 1103 68-69 0.5164674163811291 1103 70-71 0.5849267779032026 1103 72-73 0.7019418663775632 1103 74-75 0.8648828646891396 1103 76-77 0.8609880602355844 1103 78-79 0.6202367635732919 1103 80-81 0.670218216394538 1103 82-83 0.5540060453490661 1103 84-85 0.5084915078272871 1103 86-87 0.7540152175927339 1103 88-89 0.6868158964037967 1103 90-91 0.7435916317221505 1103 92-93 0.48116829424969865 1103 94-95 -0.01636753914430855 1103 96-97 0.042087152411337314 1103 98-99 0.4079279837104117 1103 100-101 0.7255858922672118 1104 1 0.011145996743103126 1104 2 -0.0445449496702679 1104 3 -0.0963736984837702 1104 4 -0.04864190746308594 1104 5 -0.08965270752024423 1104 6 0.034645093773363556 1104 7 0.00632404676695586 1104 8 -0.034615945438439155 1104 9 -0.051885673184443704 1104 10-11 -0.02342153122263113 1104 12-13 0.009273578500419433 1104 14-15 0.08048787978792404 1104 16-17 0.013581573033512484 1104 18-19 0.17405496537465837 1104 20-21 0.1282964976312897 1104 22-23 -0.03781269755231875 1104 24-25 0.10847785965740542 1104 26-27 0.12166546886751917 1104 28-29 0.11869215763677943 1104 30-31 0.09830081793160872 1104 32-33 0.1508064253577004 1104 34-35 0.20897596895582637 1104 36-37 0.2531187239840662 1104 38-39 0.2793651069413059 1104 40-41 0.4625232596867548 1104 42-43 0.751340772035924 1104 44-45 0.8016883767062879 1104 46-47 0.8406699799608575 1104 48-49 0.8001426513376444 1104 50-51 0.6909189350856906 1104 52-53 0.7719792461662536 1104 54-55 0.7847769464780683 1104 56-57 0.7736755764860064 1104 58-59 0.6465088191002266 1104 60-61 0.8781019424481045 1104 62-63 0.9841535308390377 1104 64-65 1.009579359376346 1104 66-67 0.8173830899838777 1104 68-69 0.7125448368097302 1104 70-71 1.0310458066563655 1104 72-73 1.1218675502917854 1104 74-75 1.1137205451878636 1104 76-77 1.0900292235319782 1104 78-79 1.2146469565273783 1104 80-81 1.2991370873978774 1104 82-83 1.1654774604300933 1104 84-85 1.1952296684498904 1104 86-87 1.1553664027063597 1104 88-89 0.9434578513437906 1104 90-91 1.1408073111860908 1104 92-93 1.0974789383827463 1104 94-95 1.2714309101315244 1104 96-97 1.2876061167620492 1104 98-99 1.4028065416744262 1104 100-101 1.666825892701386 1105 1 0.15406289927715378 1105 2 0.14773552717032956 1105 3 0.08308044889726673 1105 4 0.11874762335506972 1105 5 0.10443215478870371 1105 6 0.07854972565375817 1105 7 0.19239427419860533 1105 8 0.1830424741946075 1105 9 0.09034443903292555 1105 10-11 0.032727739530919564 1105 12-13 0.039723363722359295 1105 14-15 0.07760960400622707 1105 16-17 -0.05971872499186048 1105 18-19 0.17898216560109148 1105 20-21 0.08559582998785942 1105 22-23 0.1459047527490398 1105 24-25 0.23231239730183972 1105 26-27 0.18492614117280226 1105 28-29 0.10423105910525976 1105 30-31 0.16814427136306875 1105 32-33 0.2722299127540566 1105 34-35 0.4158392935204418 1105 36-37 0.38601681262579746 1105 38-39 0.5169383645750756 1105 40-41 0.3605109640588964 1105 42-43 0.5924851311981243 1105 44-45 0.8042690433507502 1105 46-47 0.7640533171790693 1105 48-49 0.8045277034504537 1105 50-51 0.6684130245266005 1105 52-53 0.8431775495860521 1105 54-55 0.8829369727842149 1105 56-57 0.9040377439130012 1105 58-59 1.1251142601444784 1105 60-61 1.285774430876728 1105 62-63 1.3016379635918653 1105 64-65 1.418527612847754 1105 66-67 1.330647508418167 1105 68-69 0.9691588356775647 1105 70-71 0.8979125443607359 1105 72-73 1.195286475735493 1105 74-75 0.976469662766366 1105 76-77 1.1440338354330137 1105 78-79 1.5026295828135474 1105 80-81 1.554191239773754 1105 82-83 1.4416067102053276 1105 84-85 1.3040130922452988 1105 86-87 1.4386289064524895 1105 88-89 1.4612602888250592 1105 90-91 1.2910260434960357 1105 92-93 1.3092528137115735 1105 94-95 1.1710700330378536 1105 96-97 1.2787558869994697 1105 98-99 1.410913789185333 1105 100-101 1.559924898728493 1106 1 -0.31971069649917894 1106 2 -0.24991746564356276 1106 3 -0.19291287829051385 1106 4 -0.005791460259352732 1106 5 -0.09409317466175793 1106 6 -0.3154499810358189 1106 7 -0.4820180574097819 1106 8 -0.2215478339908472 1106 9 -0.38301799837672235 1106 10-11 -0.8670301484244973 1106 12-13 -0.5025591675466359 1106 14-15 -0.35772199390707726 1106 16-17 -0.2944790222829283 1106 18-19 -0.8382622615007875 1106 20-21 -0.5080441786019563 1106 22-23 -0.5124691290049554 1106 24-25 -0.4587295879907387 1106 26-27 -0.6179934760570873 1106 28-29 -0.5151587242640332 1106 30-31 -0.7770819704923255 1106 32-33 -0.5397129283764457 1106 34-35 -0.3249279751309544 1106 36-37 -0.6967399387517119 1106 38-39 -0.29723507616574096 1106 40-41 -0.14966258667332966 1106 42-43 -0.3592659582406057 1106 44-45 -0.4063068926237676 1106 46-47 -0.2793173433979135 1106 48-49 -0.0032740920283700348 1106 50-51 -0.414187905686461 1106 52-53 -0.08353739981789943 1106 54-55 -0.3999759925842028 1106 56-57 -0.22625035501641122 1106 58-59 -0.2497521788574275 1106 60-61 -0.15577953824187318 1106 62-63 -0.3297991527476256 1106 64-65 -0.24165916304212942 1106 66-67 0.04394769592732928 1106 68-69 -0.020059387280259244 1106 70-71 0.32975191500035805 1106 72-73 0.10714734707696749 1106 74-75 0.1447807446628282 1106 76-77 0.18603353164721526 1106 78-79 0.06377367679858992 1106 80-81 -0.3297396699075854 1106 82-83 -0.4280364462338859 1106 84-85 -0.71477710699628 1106 86-87 -0.14113647139419427 1106 88-89 0.378418425046803 1106 90-91 0.6475900427837082 1106 92-93 0.37676331527240237 1106 94-95 0.3987352296605948 1106 96-97 0.5659257818267314 1106 98-99 0.49983202366606605 1106 100-101 0.7769111079007303 1107 1 0.11254540630153542 1107 2 0.0954038857310131 1107 3 0.13222279458747366 1107 4 0.10015567193659791 1107 5 0.20096641564250461 1107 6 0.129102612447916 1107 7 0.12605918854647058 1107 8 0.20416093698804616 1107 9 0.1709718749071243 1107 10-11 0.2266204930884257 1107 12-13 0.2717941966780302 1107 14-15 0.2441384266292701 1107 16-17 0.12447585037295283 1107 18-19 0.24526910025168291 1107 20-21 0.20185217459041382 1107 22-23 0.22508482616515124 1107 24-25 0.4580216332304232 1107 26-27 0.38075059961565927 1107 28-29 0.44253149869557973 1107 30-31 0.3687101872517644 1107 32-33 0.2383627641997208 1107 34-35 0.2779404899312041 1107 36-37 0.22800788486362933 1107 38-39 0.337550392128783 1107 40-41 0.6544784932893961 1107 42-43 0.630963446252963 1107 44-45 0.8512757505020332 1107 46-47 0.9763822395030104 1107 48-49 0.8393040562103593 1107 50-51 0.6540583612437274 1107 52-53 0.7129574145366533 1107 54-55 0.681493009220361 1107 56-57 0.6025473287947207 1107 58-59 0.8246406808822613 1107 60-61 1.073949677863041 1107 62-63 1.2102416978436494 1107 64-65 0.9085122045272698 1107 66-67 0.9751883178081755 1107 68-69 0.9112869966491992 1107 70-71 1.1702999276653685 1107 72-73 1.0464234058542274 1107 74-75 1.0975241130518718 1107 76-77 0.8592461530256905 1107 78-79 1.0023151850976042 1107 80-81 1.2509220700103185 1107 82-83 1.4981813046039463 1107 84-85 1.4101039106990285 1107 86-87 1.3244288928569112 1107 88-89 1.0298952929037348 1107 90-91 1.059739788624288 1107 92-93 1.0400632233916234 1107 94-95 1.1042647670177352 1107 96-97 1.311710998936384 1107 98-99 1.3850000531207947 1107 100-101 1.8920223563556107 1108 1 0.20014267718481094 1108 2 0.27042693570335175 1108 3 0.16708541675232524 1108 4 0.18219882148481759 1108 5 0.022338349996189777 1108 6 0.16647491560115668 1108 7 0.03356610353817757 1108 8 0.31037152153534464 1108 9 0.17906886919072917 1108 10-11 0.18509550691840815 1108 12-13 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0.6130760592320144 2316 52-53 0.7317217384480372 2316 54-55 0.46251223509252526 2316 56-57 0.7136372192963805 2316 58-59 0.7497107088934669 2316 60-61 0.8297110733303157 2316 62-63 0.704432556005095 2316 64-65 1.1306442391592952 2316 66-67 0.48013802496376456 2316 68-69 0.5012411601327749 2316 70-71 0.9064375645382938 2316 72-73 0.7111006403844016 2316 74-75 0.7385509783434436 2316 76-77 0.6658256029874963 2316 78-79 1.0442205836206533 2316 80-81 0.6686232413879587 2316 82-83 -0.1561031364451999 2316 84-85 0.5819590163776667 2316 86-87 1.0963922236437718 2316 88-89 0.9697348650962461 2316 90-91 0.6077885535848147 2316 92-93 0.6431158080618573 2316 94-95 0.7988948917949195 2316 96-97 0.8808795754579037 2316 98-99 1.7300471628737881 2316 100-101 2.0973794992127566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2689.0 3 473.0 4 359.0 5 394.0 6 423.0 7 506.0 8 678.0 9 903.0 10 1128.0 11 1746.0 12 2722.0 13 3971.0 14 4419.0 15 6249.0 16 8716.0 17 12460.0 18 9944.0 19 5441.0 20 5121.0 21 5185.0 22 5444.0 23 5852.0 24 6576.0 25 7347.0 26 8349.0 27 9389.0 28 11409.0 29 13069.0 30 16216.0 31 20195.0 32 25529.0 33 33614.0 34 45093.0 35 64291.0 36 93419.0 37 131967.0 38 110129.0 39 13899.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77073345218109 19.764208364343368 15.538334169883056 20.92672401359249 2 14.853770646857987 21.41390606256344 40.996124388668456 22.736198901910125 3 18.003330185755168 27.528382264958807 31.271309224326217 23.19697832495981 4 11.704492700551027 17.219375618139196 38.55905445968507 32.51707722162471 5 12.021464790275676 38.889217287749005 34.04911469274122 15.040203229234098 6 28.836696845499567 37.75637346377819 18.172627923544393 15.234301767177852 7 26.17160459642808 35.404841017121235 21.452449351608287 16.971105034842402 8 26.20846280848732 33.797304994196146 20.55731393429031 19.43691826302622 9 27.358282447425907 15.918649835266638 20.985660627644926 35.73740708966253 10-11 23.77271619589245 27.87320314403831 27.437444394616893 20.916636265452347 12-13 25.661054832546238 25.77353185046325 28.327409063052023 20.2380042539385 14-15 22.642794786268286 26.109893215242693 28.28905775229368 22.95825424619535 16-17 19.94234802728014 29.915576919887837 28.494033280387153 21.648041772444866 18-19 19.92894594744624 27.075299561030718 33.03357404912217 19.96218044240087 20-21 21.24102345255928 26.03952059722064 33.02360249879976 19.695853451420316 22-23 20.77725764851917 26.331075168209033 32.960680823088936 19.930986360182864 24-25 20.134747749819258 26.510361967890912 33.16478451820868 20.190105764081153 26-27 19.84090311093169 26.67731157255056 33.132089345122026 20.34969597139572 28-29 19.87578580188744 26.773898867954998 33.176755765671516 20.173559564486045 30-31 20.050888970763715 26.678351854909472 33.02397145949251 20.246787714834298 32-33 19.66324863478202 26.972914825106457 32.766096738329495 20.59773980178203 34-35 20.131079120505106 26.918306657936714 32.66168924629145 20.28892497526673 36-37 20.339250623903393 26.782648060817426 32.37997338588875 20.498127929390428 38-39 20.131625422592574 27.07771378318568 32.305967836109765 20.484692958111975 40-41 20.083584594082044 27.17976958734612 32.286027115257504 20.450618703314337 42-43 20.023817367503277 27.217181376029664 31.895448676961095 20.863552579505964 44-45 20.122919319415743 27.545809942275216 31.47211891622192 20.859151822087117 46-47 20.322782080994045 27.749207177870495 30.88754256146084 21.04046817967462 48-49 20.446308429613328 28.11399858951191 30.159855316804567 21.279837664070193 50-51 20.657897583454123 28.443704072287247 29.75430701887228 21.144091325386352 52-53 20.59679336630178 28.63342989203506 29.442012845000203 21.327763896662955 54-55 20.487785179533848 28.579896420283855 29.405418970000536 21.52689943018176 56-57 20.61733796878638 28.785774166551935 29.070936704152516 21.52595116050917 58-59 20.659582840266538 28.21478349983496 29.51114679289177 21.614486867006732 60-61 20.577671442160792 28.230448819578562 29.7868323196218 21.40504741863884 62-63 20.58925701436386 28.17860772174615 30.0634950378736 21.16864022601639 64-65 20.669451785187945 28.1634000257997 29.94487656140437 21.222271627607984 66-67 20.590569027880974 28.206074329568736 29.72613411724178 21.477222525308505 68-69 20.604438371328744 28.330147830398634 29.66798699276992 21.397426805502704 70-71 20.463671899773416 28.20472536279094 29.684932573062667 21.646670164372978 72-73 20.4335576941788 28.32251168831356 29.37159503768296 21.87233557982468 74-75 20.277029793171845 28.540281613801614 29.341257805787958 21.84143078723858 76-77 20.216249961252633 28.329480222982994 29.56060124380853 21.893668571955843 78-79 20.278096144281296 28.131392859459808 29.358966041707212 22.23154495455168 80-81 20.127492934478234 28.442428160888472 29.079316203092038 22.35076270154126 82-83 20.23090600227317 28.065983851426786 29.355289511787824 22.347820634512225 84-85 20.140410406024053 27.810658563867204 29.234222027468025 22.81470900264071 86-87 20.20105841462694 27.72399049127911 29.21978321384963 22.85516788024432 88-89 20.167196815216382 27.298289469036945 29.36698605323686 23.167527662509812 90-91 19.790246740131007 27.27232097482139 29.66374457649636 23.27368770855124 92-93 19.734018744535607 27.328993773204523 29.661068869554434 23.275918612705432 94-95 19.44544950015496 27.37356118867258 29.569059339930664 23.611929971241793 96-97 19.507889482372107 27.392109796798074 29.348802341255254 23.751198379574568 98-99 19.528626082142246 27.37584695270041 29.36708240248735 23.728444562669996 100-101 19.534342711484037 27.076788698654568 29.713668404215376 23.675200185646016 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1312.0 1 821.5 2 368.5 3 412.5 4 377.0 5 423.5 6 720.0 7 1333.5 8 2169.0 9 2533.5 10 2187.0 11 1979.5 12 2050.0 13 2036.0 14 2086.5 15 2150.5 16 2192.0 17 2251.0 18 2347.0 19 2517.0 20 2702.0 21 2955.0 22 3273.0 23 3693.0 24 4082.0 25 4629.5 26 5493.5 27 6428.0 28 7371.5 29 8493.5 30 9866.5 31 11430.5 32 12896.5 33 14210.0 34 15760.5 35 17297.0 36 19326.5 37 21061.0 38 21874.5 39 22553.0 40 22654.5 41 23074.5 42 23533.0 43 24291.5 44 25516.5 45 26038.5 46 25780.0 47 25536.5 48 25481.0 49 24510.5 50 22767.0 51 20690.0 52 18817.0 53 17810.0 54 17107.5 55 16043.0 56 14551.0 57 12819.5 58 11666.0 59 10593.0 60 9260.0 61 8052.0 62 6608.5 63 4698.5 64 3075.0 65 2113.0 66 1468.0 67 1051.5 68 774.0 69 528.5 70 356.0 71 277.5 72 207.0 73 147.5 74 110.0 75 97.5 76 72.5 77 39.0 78 26.5 79 15.5 80 11.0 81 7.0 82 6.5 83 6.5 84 4.5 85 3.0 86 1.0 87 2.0 88 2.0 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2862016297672706 2 0.25110957064002737 3 0.2385972380823628 4 0.2444938545750553 5 0.24636351346298221 6 0.27210727815059094 7 0.2741207569529737 8 0.26017022525075 9 0.25441742867251343 10-11 0.26045786507966184 12-13 0.26448482268442747 14-15 0.24772980265031336 16-17 0.2649881923850232 18-19 0.2527634996562704 20-21 0.24463767448951124 22-23 0.24204891602930476 24-25 0.23694330906611974 26-27 0.2461477835912983 28-29 0.26383763306937585 30-31 0.2651320122994791 32-33 0.2639095430266038 34-35 0.27555895609753295 36-37 0.24406239483168754 38-39 0.24147363637148106 40-41 0.23665566923720796 42-43 0.2448534043611951 44-45 0.2662106616578985 46-47 0.2739769370385179 48-49 0.2785791743011071 50-51 0.2831814115636964 52-53 0.30576113813327505 54-55 0.2782915344721953 56-57 0.24082644675642947 58-59 0.21961300937418204 60-61 0.21486695219713683 62-63 0.2242152466367713 64-65 0.21680852104229167 66-67 0.21939727950249813 68-69 0.2223455877488444 70-71 0.21946918945972613 72-73 0.22515007608073473 74-75 0.22982422330055197 76-77 0.24715452299248972 78-79 0.24003543722692194 80-81 0.2342107306914574 82-83 0.2255815358241025 84-85 0.22464670638013906 86-87 0.23514556013542082 88-89 0.23593656966492838 90-91 0.24298374547326818 92-93 0.23277253154689823 94-95 0.22996804321500788 96-97 0.24003543722692194 98-99 0.24801744247922522 100-101 0.21867817993021857 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 695314.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.359599374173506 #Duplication Level Percentage of deduplicated Percentage of total 1 75.259406361332 40.15811772578801 2 13.146995153896999 14.030367887722885 3 4.715856808815224 7.54908690073046 4 2.212987739998454 4.723365569051007 5 1.2120557739281435 3.2337405257979768 6 0.7397419866021518 2.3683401627207625 7 0.49987638665400386 1.867124260992697 8 0.36634055727130094 1.5638228296406467 9 0.28691080141818603 1.3778500877817714 >10 1.4317870210604746 13.931339301623696 >50 0.09184412104791546 3.3387166199186753 >100 0.03430638487201666 3.034574368495805 >500 5.402580294697568E-4 0.22557872448753546 >1k 8.103870442046352E-4 0.8166958804769177 >5k 5.402580294697568E-4 1.7812791547712228 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7290 1.0484471763836194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5068 0.7288793264625767 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3491 0.5020753213655988 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1107 0.1592086453026978 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1068 0.1535996686389171 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 885 0.12728062429348466 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.4381991445591488E-4 0.0 8 0.0 0.0 0.0 1.4381991445591488E-4 0.0 9 0.0 0.0 0.0 1.4381991445591488E-4 0.0 10-11 0.0 0.0 0.0 1.4381991445591488E-4 0.0 12-13 0.0 0.0 0.0 1.4381991445591488E-4 0.0 14-15 0.0 0.0 0.0 1.4381991445591488E-4 0.0 16-17 0.0 0.0 0.0 1.4381991445591488E-4 0.0 18-19 0.0 0.0 0.0 2.1572987168387233E-4 0.0 20-21 0.0 0.0 0.0 2.8763982891182976E-4 0.0 22-23 0.0 0.0 0.0 3.595497861397872E-4 0.0 24-25 0.0 0.0 0.0 8.629194867354892E-4 0.0 26-27 0.0 0.0 0.0 0.0016539290162430212 0.0 28-29 0.0 0.0 0.0 0.004242687476449489 0.0 30-31 0.0 0.0 0.0 0.008844924739038765 0.0 32-33 0.0 0.0 0.0 0.01488536114618719 0.0 34-35 0.0 0.0 0.0 0.02106961746779153 0.0 36-37 0.0 0.0 0.0 0.031065101522477614 0.0 38-39 0.0 0.0 0.0 0.04746057177045191 0.0 40-41 0.0 0.0 0.0 0.06917737885329506 0.0 42-43 0.0 0.0 0.0 0.09643125264269092 0.0 44-45 0.0 0.0 0.0 0.12001771861346097 0.0 46-47 0.0 0.0 0.0 0.17006704884411933 0.0 48-49 0.0 0.0 0.0 0.23248489171798642 0.0 50-51 0.0 0.0 0.0 0.2724668279367307 0.0 52-53 0.0 0.0 0.0 0.3381925288430838 0.0 54-55 0.0 0.0 0.0 0.4417428672513426 0.0 56-57 0.0 0.0 0.0 0.6010234225112683 0.0 58-59 0.0 0.0 0.0 0.77029946182588 0.0 60-61 0.0 0.0 0.0 0.9807079966748835 0.0 62-63 0.0 0.0 0.0 1.194424389556373 0.0 64-65 0.0 0.0 0.0 1.4214441245250349 0.0 66-67 0.0 0.0 0.0 1.7349715380389292 0.0 68-69 0.0 0.0 0.0 2.12501114604337 0.0 70-71 0.0 0.0 0.0 2.3969026943222773 0.0 72-73 0.0 0.0 0.0 2.7347356733792214 0.0 74-75 0.0 0.0 0.0 3.1101775600663872 0.0 76-77 0.0 0.0 0.0 3.4903655039305983 0.0 78-79 0.0 0.0 0.0 3.9446207037396057 0.0 80-81 0.0 0.0 0.0 4.573689009569777 0.0 82-83 0.0 0.0 0.0 5.243314531276517 0.0 84-85 0.0 0.0 0.0 6.049497061759148 0.0 86-87 0.0 0.0 0.0 7.015679247073984 0.0 88-89 0.0 0.0 0.0 8.105834198649818 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2840 0.0 51.043224 1 GTATCAA 5340 0.0 43.523537 1 TATCAAC 6835 0.0 33.924427 2 ATCAACG 6910 0.0 33.561058 3 TCAACGC 7055 0.0 32.87603 4 CAACGCA 7140 0.0 32.42042 5 AACGCAG 7300 0.0 31.700684 6 GGGCGCG 80 4.483493E-4 29.696905 7 GTCGGGA 210 1.8189894E-12 29.414078 2 GTACATG 5355 0.0 27.869345 1 CGCAGAG 8235 0.0 27.578087 8 ACGCAGA 8340 0.0 27.460735 7 TACATGG 5525 0.0 26.574028 2 ACATGGG 5345 0.0 26.228184 3 GCAGAGT 8525 0.0 25.97116 9 GAGTACT 5000 0.0 23.893171 12-13 AGTACTT 5170 0.0 23.291273 12-13 CATGGGA 3740 0.0 23.256056 4 ATGGGAT 1310 0.0 22.859 5 TGGTATC 1315 0.0 22.402533 2 >>END_MODULE