FastQCFastQC Report
Thu 26 May 2016
SRR937336_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937336_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695314
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80781.1617772689748804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64290.9246182300370769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46020.6618592463261204No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.18826026802279258No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.17100187828808278No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7070.10168067952033182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36750.053.276261
GTATCAA63400.039.801471
CGTCAAG753.0688947E-431.681271
TATCAAC81000.031.078932
ATCAACG81850.030.814213
TCAACGC83000.030.3872644
CAACGCA83950.030.0433945
AACGCAG86500.029.1028146
CGTATAG908.919929E-426.401061
CGCAGAG96850.025.556838
ACGCAGA97100.025.4910327
GAGTACT58600.025.41609612-13
GCAGAGT100100.024.3948389
TAAGGTG2751.8189894E-1224.1807925
GTACTTT61700.024.05692114-15
CTACACT2853.6379788E-1223.3323424
AGTACTT60400.022.81024612-13
CAGAGTA98050.021.68274910-11
GTACATG53700.021.5928211
TAGACCC1100.002876943221.5899944
Produced by FastQC (version 0.11.5)