##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937336_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 695314 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.204821131172395 33.0 31.0 34.0 31.0 34.0 2 32.39939365524065 34.0 31.0 34.0 31.0 34.0 3 32.428258312071954 34.0 31.0 34.0 31.0 34.0 4 36.1013441409205 37.0 35.0 37.0 35.0 37.0 5 36.031985548974994 37.0 35.0 37.0 35.0 37.0 6 36.08890084192178 37.0 35.0 37.0 35.0 37.0 7 36.022812714831 37.0 35.0 37.0 35.0 37.0 8 36.00978550697958 37.0 35.0 37.0 35.0 37.0 9 37.6995601987016 39.0 37.0 39.0 35.0 39.0 10-11 37.6265694348165 39.0 37.0 39.0 35.0 39.0 12-13 37.5250850694794 39.0 37.0 39.0 35.0 39.0 14-15 38.632346680780195 40.0 38.0 41.0 34.0 41.0 16-17 38.628608801203484 40.0 38.0 41.0 34.5 41.0 18-19 38.71995889626845 40.0 38.0 41.0 35.0 41.0 20-21 38.67299522230244 40.0 38.0 41.0 34.0 41.0 22-23 38.52620470751344 40.0 38.0 41.0 34.0 41.0 24-25 38.449855029526226 40.0 38.0 41.0 34.0 41.0 26-27 38.35379267496411 40.0 38.0 41.0 34.0 41.0 28-29 38.20512099569403 40.0 38.0 41.0 34.0 41.0 30-31 38.16992610532795 40.0 37.0 41.0 34.0 41.0 32-33 38.000404853059194 40.0 37.0 41.0 33.5 41.0 34-35 37.833816807945766 40.0 36.5 41.0 33.0 41.0 36-37 37.61612523262872 40.0 36.0 41.0 33.0 41.0 38-39 37.456354970560064 40.0 36.0 41.0 33.0 41.0 40-41 37.21313536042709 40.0 36.0 41.0 33.0 41.0 42-43 36.90183068369111 40.0 35.5 41.0 31.5 41.0 44-45 36.439520130473426 39.5 35.0 41.0 30.5 41.0 46-47 35.83565266915379 39.0 35.0 41.0 29.0 41.0 48-49 35.29682057315112 39.0 35.0 41.0 26.5 41.0 50-51 34.97995366122356 39.0 35.0 41.0 24.5 41.0 52-53 34.70695038500591 39.0 35.0 40.0 23.5 41.0 54-55 34.46001015368596 38.5 34.5 40.0 23.0 41.0 56-57 34.185018567150955 38.0 34.0 40.0 22.0 41.0 58-59 33.90298627670376 38.0 34.0 40.0 20.5 41.0 60-61 33.57280739349416 37.5 33.5 40.0 18.5 41.0 62-63 33.20354688673031 37.0 33.0 40.0 16.5 41.0 64-65 32.807213719269285 36.5 33.0 39.5 12.5 41.0 66-67 32.39534728194744 36.0 32.0 39.0 8.0 40.5 68-69 31.94759993326756 35.5 32.0 39.0 6.5 40.0 70-71 31.539751824355612 35.0 31.0 38.0 2.0 40.0 72-73 31.0839455267692 35.0 31.0 37.5 2.0 40.0 74-75 30.610357334959456 35.0 30.5 37.0 2.0 39.0 76-77 29.343672786683427 33.5 28.5 35.5 2.0 38.5 78-79 29.90734402011178 34.0 30.0 36.0 2.0 38.5 80-81 29.698003348127607 34.0 30.0 36.0 2.0 37.5 82-83 29.365016381088257 34.0 30.0 35.0 2.0 37.0 84-85 29.021365886491573 34.0 29.5 35.0 2.0 36.5 86-87 28.616939972444104 34.0 29.0 35.0 2.0 36.0 88-89 28.188996625984807 34.0 29.0 35.0 2.0 36.0 90-91 27.709662109492978 34.0 27.0 35.0 2.0 35.0 92-93 27.389587294373477 33.5 27.0 35.0 2.0 35.0 94-95 26.99740405054407 33.0 26.0 35.0 2.0 35.0 96-97 26.558789266432143 33.0 25.0 35.0 2.0 35.0 98-99 25.90010484471764 33.0 24.5 35.0 2.0 35.0 100-101 23.652564884354405 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2518432469954561 1101 2 -0.20034118945206103 1101 3 -0.1417489530323195 1101 4 -0.07401883791817454 1101 5 -0.05782839392823291 1101 6 -0.22037278338433453 1101 7 -0.29905447666955354 1101 8 -0.13682389581404664 1101 9 -0.2769979979994659 1101 10-11 -0.26618571339111696 1101 12-13 -0.21196951174212586 1101 14-15 -0.4203080445577996 1101 16-17 -0.30193988488952783 1101 18-19 -0.2273370826457679 1101 20-21 -0.1171636624063197 1101 22-23 -0.1728083750579401 1101 24-25 -0.1352236771202513 1101 26-27 -0.2702731951803088 1101 28-29 -0.24582395375603738 1101 30-31 -0.19539088222158085 1101 32-33 -0.1631207970892845 1101 34-35 -0.01660912199096032 1101 36-37 0.06003305056452746 1101 38-39 -0.04236696268156237 1101 40-41 -0.019086159850026263 1101 42-43 -0.1318267668338251 1101 44-45 0.14718005281041968 1101 46-47 -0.11292832074989434 1101 48-49 0.04952279161946649 1101 50-51 0.05022657732894942 1101 52-53 0.16189219492530071 1101 54-55 0.188129056144426 1101 56-57 0.13152275734128693 1101 58-59 0.2779854366081125 1101 60-61 0.25305689214713567 1101 62-63 0.32259301850112365 1101 64-65 0.28609264209113405 1101 66-67 0.1333578396793058 1101 68-69 0.4833485224255867 1101 70-71 0.5547410667909176 1101 72-73 0.6113642016893728 1101 74-75 0.5316085443649357 1101 76-77 0.6091964645212862 1101 78-79 0.4940566608314185 1101 80-81 0.6730447354982161 1101 82-83 0.6580955730482749 1101 84-85 0.6105659634760805 1101 86-87 0.4284820805714027 1101 88-89 0.5390199971490759 1101 90-91 0.27895551090577797 1101 92-93 0.48202361249723324 1101 94-95 0.3291007982194998 1101 96-97 0.33388220850244466 1101 98-99 0.5379976959566406 1101 100-101 1.1286560824729222 1102 1 -0.1327661160549667 1102 2 -0.0033033582824657515 1102 3 -0.05841023482754082 1102 4 -0.08600556176238427 1102 5 -0.022822813243273288 1102 6 -4.4533228876275643E-4 1102 7 -0.07411175212041599 1102 8 -0.14520079765273408 1102 9 -0.1271228291103128 1102 10-11 -0.20590550426229015 1102 12-13 -0.20444072715656603 1102 14-15 -0.1551094896614842 1102 16-17 -0.12619897553160087 1102 18-19 -0.17148690935081135 1102 20-21 -0.1283999309841235 1102 22-23 -0.03726528629567838 1102 24-25 -0.0904504291616135 1102 26-27 -0.16929070090785814 1102 28-29 -0.24930821035005835 1102 30-31 0.03690586335793 1102 32-33 0.052541835819127414 1102 34-35 -0.15412821380791542 1102 36-37 -0.25618911944076217 1102 38-39 -0.07934634351924785 1102 40-41 -0.12794476291646362 1102 42-43 -0.15262509850274597 1102 44-45 -0.3457082453743894 1102 46-47 -0.35878980227489166 1102 48-49 0.019365798140057677 1102 50-51 -0.11537437432469488 1102 52-53 0.025228563367988954 1102 54-55 -0.07585863697664053 1102 56-57 -0.08571495046579969 1102 58-59 0.0124278086929408 1102 60-61 0.096615606827271 1102 62-63 0.13116451001260998 1102 64-65 0.23939552642409723 1102 66-67 0.15730778974685933 1102 68-69 0.15342723945548897 1102 70-71 0.3267465887318224 1102 72-73 0.4354602775866887 1102 74-75 0.18977103041322252 1102 76-77 0.20334115112177287 1102 78-79 0.1734727449528961 1102 80-81 0.04676925526559472 1102 82-83 0.06682420489930152 1102 84-85 0.3430448156173007 1102 86-87 0.389525962378368 1102 88-89 0.49547523418132755 1102 90-91 0.3523907162162381 1102 92-93 0.4962514219315288 1102 94-95 0.5851690588081127 1102 96-97 0.4465253971295944 1102 98-99 0.6937817821929109 1102 100-101 1.048117986955095 1103 1 -0.17032565120451437 1103 2 -0.17537264132050723 1103 3 -0.18678865611413897 1103 4 -0.13976500800611547 1103 5 -0.1171963447095834 1103 6 -0.08302772893573973 1103 7 -0.11909174831016855 1103 8 -0.13939651747237747 1103 9 -0.15839386486315732 1103 10-11 -0.19716415670402654 1103 12-13 -0.27080019245154574 1103 14-15 -0.32115857837679584 1103 16-17 -0.28854656554874936 1103 18-19 -0.4277984290053567 1103 20-21 -0.6129541317842708 1103 22-23 -0.4674519885246724 1103 24-25 -0.4625419386554839 1103 26-27 -0.35240335728685324 1103 28-29 -0.5968942584862091 1103 30-31 -0.6311412249763535 1103 32-33 -0.44687233830927653 1103 34-35 -0.3313913432928999 1103 36-37 -0.422731952996358 1103 38-39 -0.33418853568284135 1103 40-41 -0.39709761742843597 1103 42-43 -0.4043270099779477 1103 44-45 -0.5895364348708014 1103 46-47 -0.2664103014175865 1103 48-49 -0.2588451309257991 1103 50-51 -0.09470535800412705 1103 52-53 -0.12281074570675798 1103 54-55 -0.16709744436852958 1103 56-57 -0.2913676144652868 1103 58-59 -0.23642673502967426 1103 60-61 -0.22862729658289993 1103 62-63 -0.07329110057988686 1103 64-65 -0.29249467488408953 1103 66-67 0.010022592566450328 1103 68-69 -0.323107973600969 1103 70-71 0.005564776323108589 1103 72-73 0.02071223033959413 1103 74-75 0.15547243385514875 1103 76-77 0.33862882496440605 1103 78-79 0.22963899884252825 1103 80-81 0.018401153404919768 1103 82-83 0.19289028097728433 1103 84-85 0.16584360268785403 1103 86-87 0.05426210259555475 1103 88-89 0.09834324078575918 1103 90-91 -0.013252739680122971 1103 92-93 0.08353471398715939 1103 94-95 0.043021348133066795 1103 96-97 0.16252050730931344 1103 98-99 0.3699129500344043 1103 100-101 0.9059956779438529 1104 1 0.06672240098317417 1104 2 0.0389210845059651 1104 3 0.07232447149316812 1104 4 0.03213345320950367 1104 5 0.003679456250658575 1104 6 0.05959294425076678 1104 7 0.014981127765771873 1104 8 0.10272955936915196 1104 9 0.16430220984368304 1104 10-11 -0.017286846989748028 1104 12-13 0.0367260298382206 1104 14-15 0.11346479392364017 1104 16-17 -0.08764891117282758 1104 18-19 -0.054263313388482004 1104 20-21 -0.09234715678183392 1104 22-23 -0.05949955226681425 1104 24-25 -0.05859113778569025 1104 26-27 -0.22825858926231035 1104 28-29 -0.08714524713618488 1104 30-31 -0.019456341099761687 1104 32-33 0.19937639987306 1104 34-35 0.28731630214628723 1104 36-37 0.20032466688807915 1104 38-39 0.260061555674568 1104 40-41 0.07352871449021592 1104 42-43 0.06613379317126089 1104 44-45 0.3104507208203202 1104 46-47 0.40874627251678675 1104 48-49 0.3915653442412719 1104 50-51 0.5131687460600958 1104 52-53 0.5417230420618466 1104 54-55 0.5138227870461165 1104 56-57 0.5607021283229017 1104 58-59 0.6365801357710481 1104 60-61 0.6279786581110613 1104 62-63 0.749978445382844 1104 64-65 0.8631828714549172 1104 66-67 0.7932640257742278 1104 68-69 0.8921644523814436 1104 70-71 0.9570087660910787 1104 72-73 0.9855170149351089 1104 74-75 1.1219876824015707 1104 76-77 1.149711654475201 1104 78-79 1.0398842346893673 1104 80-81 1.0894907849722522 1104 82-83 1.1196822429726012 1104 84-85 1.0498287394706978 1104 86-87 1.0969848066301147 1104 88-89 0.8380053811903387 1104 90-91 0.5997409973625061 1104 92-93 1.0089436514552723 1104 94-95 1.1696924650814005 1104 96-97 0.872467067748115 1104 98-99 1.1557838484485856 1104 100-101 1.541919219933888 1105 1 -0.05512876947401679 1105 2 -0.1451283144484492 1105 3 -0.19965073745758133 1105 4 -0.1147314530540271 1105 5 -0.10810919547589037 1105 6 -0.14934948190886388 1105 7 -0.18338264633432289 1105 8 -0.08831885726699085 1105 9 -0.13908220958956719 1105 10-11 -0.19212701215241168 1105 12-13 -0.012765537209162403 1105 14-15 -0.11106361005890619 1105 16-17 -0.16599295566018668 1105 18-19 -0.23877577551095186 1105 20-21 -0.17079838218224808 1105 22-23 -0.1811394826720658 1105 24-25 -0.1573557476887828 1105 26-27 -0.1522246576582944 1105 28-29 -0.24443167615952888 1105 30-31 -0.2977083973083481 1105 32-33 -0.3165113494526395 1105 34-35 -0.19344732543853382 1105 36-37 -0.04764513931218062 1105 38-39 -0.24008933007682032 1105 40-41 -0.30718205105205243 1105 42-43 -0.15119684017428625 1105 44-45 -0.40855526466135217 1105 46-47 -0.5137941856764101 1105 48-49 -0.3527082813456204 1105 50-51 -0.5207732638629778 1105 52-53 -0.3873554518278013 1105 54-55 -0.36483072932029614 1105 56-57 -0.2194927120087513 1105 58-59 -0.3454842310499018 1105 60-61 -0.29034012081738325 1105 62-63 -0.04689291254729966 1105 64-65 0.05018234699686275 1105 66-67 0.11276512463873445 1105 68-69 0.07664465617085625 1105 70-71 0.2274917669901484 1105 72-73 0.30838546753408735 1105 74-75 0.330394203995926 1105 76-77 0.37423901786636193 1105 78-79 0.17029843168580427 1105 80-81 0.07662387244852553 1105 82-83 0.20741450297060382 1105 84-85 0.2859108296441839 1105 86-87 0.1793528180183479 1105 88-89 0.5494056842105515 1105 90-91 0.6275112597580268 1105 92-93 0.7840801770454959 1105 94-95 0.6262415310465705 1105 96-97 0.17499924283386292 1105 98-99 0.48519762088373497 1105 100-101 1.0013434399065808 1106 1 -0.1711212586320059 1106 2 -0.15603448653786955 1106 3 -0.09878468604445345 1106 4 -0.03880595390253205 1106 5 -0.1731062623716184 1106 6 -0.10927594479462499 1106 7 -0.17793938095124417 1106 8 -0.19339993664936372 1106 9 -0.15258103634785414 1106 10-11 -0.2731042596435671 1106 12-13 -0.22696267848003515 1106 14-15 -0.3120113693767479 1106 16-17 -0.2504035064552639 1106 18-19 -0.31700127361491326 1106 20-21 -0.17379761224238166 1106 22-23 -0.2446392260254413 1106 24-25 -0.14484815304379595 1106 26-27 -0.27676374127271686 1106 28-29 -0.2700367598577529 1106 30-31 -0.13721128851113207 1106 32-33 -0.34641068112388496 1106 34-35 -0.17699968134979116 1106 36-37 -0.21389126342439368 1106 38-39 -0.3216420683391661 1106 40-41 -0.41342065908457215 1106 42-43 -0.810531837345934 1106 44-45 -0.5073674883734114 1106 46-47 -0.847234455102992 1106 48-49 -0.6708525429366219 1106 50-51 -0.6075028002229956 1106 52-53 -0.2738646125409616 1106 54-55 -0.4587797613958955 1106 56-57 -0.7494542412036154 1106 58-59 -0.6350994706216753 1106 60-61 -0.43802912272389705 1106 62-63 -0.5360358437660011 1106 64-65 -0.45181648499911375 1106 66-67 -0.438122925056426 1106 68-69 -0.3914237899123947 1106 70-71 -0.17635217091184074 1106 72-73 -0.3055721805353038 1106 74-75 -0.2791369445239056 1106 76-77 -0.09830306694225044 1106 78-79 -0.11924282556839216 1106 80-81 0.0285949447076419 1106 82-83 -0.17934017428317262 1106 84-85 -0.22388167941701198 1106 86-87 -0.3046558713030585 1106 88-89 -0.025641193080939928 1106 90-91 0.10907787788880441 1106 92-93 0.2870787523948515 1106 94-95 0.31447087884988534 1106 96-97 0.5100808721732868 1106 98-99 0.4381815307117307 1106 100-101 1.1412086480522312 1107 1 0.052315941846565295 1107 2 -0.20700518384027333 1107 3 0.09590011000004495 1107 4 0.034140114062807925 1107 5 0.04113625000584875 1107 6 -0.09782224543646834 1107 7 -0.07811322731864578 1107 8 0.032625128956155436 1107 9 0.09220687769403924 1107 10-11 -0.0324129724533293 1107 12-13 0.045805907547475044 1107 14-15 0.1060974068902425 1107 16-17 -0.026717599909254375 1107 18-19 0.13590982677310137 1107 20-21 0.036435031213919444 1107 22-23 0.15453814354020778 1107 24-25 -0.0012332062222810691 1107 26-27 0.2188892732761758 1107 28-29 0.04124821598222894 1107 30-31 0.08523935035774599 1107 32-33 0.06155898499855539 1107 34-35 0.10472067057342116 1107 36-37 0.10602736589945749 1107 38-39 0.048949802525370956 1107 40-41 0.17232261676267768 1107 42-43 -0.03363192129455683 1107 44-45 0.1978216273897644 1107 46-47 -0.168687460397706 1107 48-49 -0.12488323376110344 1107 50-51 -0.06124842927582819 1107 52-53 -4.1683585752849694E-4 1107 54-55 0.18190689870471743 1107 56-57 0.16855620940881266 1107 58-59 0.0056442018332703014 1107 60-61 0.13999104477674962 1107 62-63 0.15736080357705617 1107 64-65 0.15365884930810836 1107 66-67 0.019731724838145226 1107 68-69 -0.05388421177077163 1107 70-71 0.03728964608015417 1107 72-73 0.21939675186292007 1107 74-75 0.025742817247081717 1107 76-77 0.11535940669986289 1107 78-79 0.14324040123171145 1107 80-81 0.4020085139784797 1107 82-83 0.0990181012666227 1107 84-85 0.03500729026232818 1107 86-87 0.11387595315838084 1107 88-89 0.08733167895359273 1107 90-91 0.2838009135240007 1107 92-93 0.31116936402999684 1107 94-95 0.10523636472734665 1107 96-97 0.0696306894032439 1107 98-99 0.4184659975718503 1107 100-101 1.1191349517347327 1108 1 0.042061470151928404 1108 2 0.02617940033822208 1108 3 0.06900355827590943 1108 4 -0.01876418245203837 1108 5 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-0.8970725631358079 2316 74-75 -0.6417355377312752 2316 76-77 -0.5574347109471951 2316 78-79 -1.228990309234291 2316 80-81 -1.6111535690365457 2316 82-83 -1.2675148755604724 2316 84-85 -0.8156579758441147 2316 86-87 -1.1205109834344391 2316 88-89 -0.9482234521897723 2316 90-91 -0.6776995957706227 2316 92-93 -0.5123282168205243 2316 94-95 -0.6469122219800632 2316 96-97 -0.9428629444139176 2316 98-99 -0.9293504017151335 2316 100-101 -0.8737826698593558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 3.0 5 19.0 6 44.0 7 70.0 8 224.0 9 426.0 10 783.0 11 1336.0 12 2173.0 13 3086.0 14 3956.0 15 5956.0 16 8626.0 17 13069.0 18 11475.0 19 5553.0 20 4348.0 21 4302.0 22 4473.0 23 4719.0 24 5292.0 25 5934.0 26 6768.0 27 8042.0 28 9870.0 29 12241.0 30 15322.0 31 19070.0 32 24039.0 33 31546.0 34 43480.0 35 63451.0 36 94836.0 37 144477.0 38 123831.0 39 12471.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.820489905291495 20.438096965039453 15.901270599206468 19.840142530462575 2 15.797898503409971 22.372338252933208 39.89837684844545 21.931386395211373 3 18.639204733401023 27.773351320410633 31.52359940976307 22.06384453642527 4 12.465447265551967 18.051700382848612 37.83556781540426 31.64728453619516 5 12.257299007622608 39.47878613548109 33.52480943477635 14.739105422119946 6 29.236287490256203 38.22862764161228 17.373014206531153 15.16207066160037 7 26.16544467679351 36.16509950899881 20.77982034016286 16.88963547404482 8 26.97687663415378 34.19347230172268 19.770060720767884 19.059590343355666 9 27.880353336765832 16.191245969446896 20.796647270154203 35.13175342363306 10-11 24.473042317693352 28.229629288722187 26.66011804772534 20.637210345859124 12-13 26.24247410343667 26.180555355791885 27.97483265397704 19.602137886794406 14-15 23.231734151764527 26.379161069674993 28.08443379537878 22.304670983181698 16-17 20.156432920953698 30.25402911490348 28.347624238833102 21.241913725309715 18-19 20.172037381672165 26.971699117233367 33.359172977963915 19.497090523130556 20-21 21.573850087873968 25.881112705914163 33.53937933077717 19.005657875434697 22-23 21.096928685972344 26.192893766045117 33.465889070264126 19.24428847771841 24-25 20.643756485421243 26.335682167437668 33.62732711116416 19.393234235976927 26-27 20.389680898876406 26.57157752808989 33.56008988764045 19.47865168539326 28-29 20.293330840368768 26.61939622315868 33.58073609572984 19.50653684074271 30-31 20.362340413597906 26.588313464583575 33.548847785098225 19.500498336720298 32-33 20.114725145761483 26.732958059236545 33.23865188964986 19.913664905352114 34-35 20.50498048363761 26.7184322478765 33.17508348746034 19.60150378102555 36-37 20.88969875480718 26.457902472839613 32.939722197453236 19.712676574899973 38-39 20.556311411280113 26.89871597211036 32.84230676300711 19.70266585360241 40-41 20.581133128342017 27.110413424726094 32.57844657233998 19.73000687459191 42-43 20.60697682446894 27.114235000345214 32.27126533336402 20.007522841821824 44-45 20.6305797752809 27.32713707865169 31.855244943820228 20.18703820224719 46-47 20.818148347365362 27.768749083148048 31.161173225334167 20.251929344152426 48-49 20.936080677219213 28.09975061626833 30.306882933466035 20.65728577304642 50-51 21.10715446546452 28.47749362158679 29.83544125387954 20.57991065906914 52-53 21.26291143282028 28.59391584233886 29.491064468714857 20.65210825612601 54-55 21.02381082503732 28.714221200781232 29.34976140276192 20.91220657141953 56-57 21.241266535694663 28.896728672225784 29.079955243242622 20.782049548836927 58-59 21.257014816327587 28.27693675087802 29.520547551178318 20.945500881616073 60-61 21.194740793368176 28.374374742921905 29.6794685566521 20.75141590705782 62-63 21.174606005344348 28.369412955873173 30.042901480482197 20.41307955830028 64-65 21.16712736979264 28.344675930586753 29.829400817472397 20.65879588214821 66-67 21.146633031982674 28.34122425263981 29.560385667482603 20.95175704789491 68-69 21.18783743747429 28.500360987985285 29.502570061871324 20.809231512669097 70-71 21.15202627877477 28.388684824410266 29.47057013090489 20.98871876591008 72-73 21.034884958450426 28.37020396540268 29.147262963207986 21.447648112938904 74-75 20.937087416620404 28.801088429112603 28.969717278812162 21.29210687545483 76-77 20.761986670770327 28.532217099037272 29.4070736386726 21.2987225915198 78-79 20.803622536005314 28.350141087336084 29.10311024947002 21.74312612718858 80-81 20.668144176587845 28.682652729558157 28.945627443140797 21.7035756507132 82-83 20.7582202247191 28.5315595505618 28.946840449438206 21.7633797752809 84-85 20.673782886268484 28.189390964932336 28.89216798765448 22.24465816114469 86-87 20.572288203602977 27.914438656491885 28.921753337341116 22.591519802564022 88-89 20.533888286443247 27.67605714828121 29.06025191496216 22.72980265031338 90-91 20.29100365480569 27.78479364946947 29.154087240686433 22.77011545503841 92-93 20.04629702873465 27.821076869751316 29.14627358538055 22.986352516133486 94-95 19.820325780870228 27.9263037994345 29.01408572242181 23.23928469727346 96-97 19.97780858719945 27.873090431085814 28.86343436202924 23.285666619685493 98-99 19.696582908285258 28.08582028106746 28.70144186758922 23.51615494305806 100-101 19.62190820853015 27.86114770981606 28.92784813039751 23.58909595125628 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 535.0 1 387.0 2 335.5 3 441.0 4 381.0 5 398.0 6 713.5 7 1484.0 8 2312.5 9 2700.0 10 2475.0 11 2106.0 12 2073.0 13 2134.5 14 2244.0 15 2297.5 16 2342.0 17 2455.5 18 2516.0 19 2611.0 20 2788.5 21 2981.0 22 3294.5 23 3696.5 24 4062.0 25 4675.5 26 5451.0 27 6347.0 28 7330.0 29 8497.5 30 9866.0 31 11344.0 32 12753.0 33 14024.5 34 15478.5 35 16943.0 36 19189.0 37 20981.0 38 21949.0 39 22535.0 40 22376.0 41 22869.0 42 23376.0 43 24244.0 44 25642.0 45 26413.5 46 25858.0 47 25185.0 48 25357.5 49 24415.0 50 22728.0 51 20888.5 52 18804.5 53 17678.0 54 17107.0 55 16014.5 56 14538.5 57 12884.0 58 11878.0 59 11061.5 60 9481.5 61 7946.5 62 6596.5 63 4800.0 64 3127.5 65 2104.0 66 1448.5 67 1085.0 68 792.5 69 495.5 70 333.0 71 237.0 72 176.0 73 146.0 74 109.5 75 79.5 76 56.5 77 38.5 78 30.0 79 20.0 80 11.0 81 8.5 82 5.0 83 2.0 84 1.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06399986193288212 2 0.0 3 0.0 4 0.0 5 0.002013478802382808 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.8763982891182976E-4 12-13 0.006903355893883915 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001294379230103234 24-25 6.47189615051617E-4 26-27 2.1572987168387233E-4 28-29 0.0034516779469419574 30-31 1.4381991445591488E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.8763982891182976E-4 40-41 0.0 42-43 0.01409435161667966 44-45 2.1572987168387233E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.1572987168387233E-4 84-85 1.4381991445591488E-4 86-87 0.0 88-89 0.0 90-91 0.009348294439634468 92-93 0.0030202182035742125 94-95 0.0 96-97 0.0 98-99 7.190995722795744E-5 100-101 0.01236851264320868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 695314.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.447246960632384 #Duplication Level Percentage of deduplicated Percentage of total 1 74.16281338434823 38.154725754798555 2 13.5229787052059 13.914400501802012 3 4.94857304324089 7.637713783750266 4 2.35700078842867 4.850448065947801 5 1.3351381704633938 3.4344591591198563 6 0.7872092315102991 2.4299848645919995 7 0.520367048608699 1.8740016441962997 8 0.37970700672672913 1.562790411820203 9 0.28880634140069256 1.3372462052854326 >10 1.5542331107170961 14.473431008814805 >50 0.10422787380247966 3.70037473735828 >100 0.035863293741143346 3.015388425161234 >500 0.0016810918940943654 0.5004746217349559 >1k 8.405459470471827E-4 1.0234360064280492 >5k 5.603639646981218E-4 2.091124809190382 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8078 1.1617772689748804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6429 0.9246182300370769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4602 0.6618592463261204 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1309 0.18826026802279258 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1189 0.17100187828808278 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 707 0.10168067952033182 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.4381991445591488E-4 0.0 8 0.0 0.0 0.0 1.4381991445591488E-4 0.0 9 0.0 0.0 0.0 1.4381991445591488E-4 0.0 10-11 0.0 0.0 0.0 1.4381991445591488E-4 0.0 12-13 0.0 0.0 0.0 1.4381991445591488E-4 0.0 14-15 0.0 0.0 0.0 1.4381991445591488E-4 0.0 16-17 0.0 0.0 0.0 1.4381991445591488E-4 0.0 18-19 0.0 0.0 0.0 2.1572987168387233E-4 0.0 20-21 0.0 0.0 0.0 2.8763982891182976E-4 0.0 22-23 0.0 0.0 0.0 3.595497861397872E-4 0.0 24-25 0.0 0.0 0.0 8.629194867354892E-4 0.0 26-27 0.0 0.0 0.0 0.0016539290162430212 0.0 28-29 0.0 0.0 0.0 0.004386507390905404 0.0 30-31 0.0 0.0 0.0 0.009204474525178552 0.0 32-33 0.0 0.0 0.0 0.015316820889554935 0.0 34-35 0.0 0.0 0.0 0.021572987168387233 0.0 36-37 0.0 0.0 0.0 0.031640381180301276 0.0 38-39 0.0 0.0 0.0 0.047820121556591696 0.0 40-41 0.0 0.0 0.0 0.06982456846834667 0.0 42-43 0.0 0.0 0.0 0.09700653230051459 0.0 44-45 0.0 0.0 0.0 0.12109636797188034 0.0 46-47 0.0 0.0 0.0 0.17186479777481828 0.0 48-49 0.0 0.0 0.0 0.23442646056314126 0.0 50-51 0.0 0.0 0.0 0.2746241266535695 0.0 52-53 0.0 0.0 0.0 0.3415003868755699 0.0 54-55 0.0 0.0 0.0 0.44548218502719633 0.0 56-57 0.0 0.0 0.0 0.6058413896455415 0.0 58-59 0.0 0.0 0.0 0.7762679882758006 0.0 60-61 0.0 0.0 0.0 0.988618091969959 0.0 62-63 0.0 0.0 0.0 1.2034131342098677 0.0 64-65 0.0 0.0 0.0 1.430720509007441 0.0 66-67 0.0 0.0 0.0 1.7466209511098583 0.0 68-69 0.0 0.0 0.0 2.1398245972323298 0.0 70-71 0.0 0.0 0.0 2.4133700745274798 0.0 72-73 0.0 0.0 0.0 2.7534322622584906 0.0 74-75 0.0 0.0 0.0 3.132397736849826 0.0 76-77 0.0 0.0 0.0 3.5139519699013686 0.0 78-79 0.0 0.0 0.0 3.9746790658608915 0.0 80-81 0.0 0.0 0.0 4.6095001682692995 0.0 82-83 0.0 0.0 0.0 5.282433548008525 0.0 84-85 0.0 0.0 0.0 6.097892462973563 0.0 86-87 0.0 0.0 0.0 7.080470118536374 0.0 88-89 0.0 0.0 0.0 8.182705942926505 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3675 0.0 53.27626 1 GTATCAA 6340 0.0 39.80147 1 CGTCAAG 75 3.0688947E-4 31.68127 1 TATCAAC 8100 0.0 31.07893 2 ATCAACG 8185 0.0 30.81421 3 TCAACGC 8300 0.0 30.387264 4 CAACGCA 8395 0.0 30.043394 5 AACGCAG 8650 0.0 29.102814 6 CGTATAG 90 8.919929E-4 26.40106 1 CGCAGAG 9685 0.0 25.55683 8 ACGCAGA 9710 0.0 25.491032 7 GAGTACT 5860 0.0 25.416096 12-13 GCAGAGT 10010 0.0 24.394838 9 TAAGGTG 275 1.8189894E-12 24.180792 5 GTACTTT 6170 0.0 24.056921 14-15 CTACACT 285 3.6379788E-12 23.332342 4 AGTACTT 6040 0.0 22.810246 12-13 CAGAGTA 9805 0.0 21.682749 10-11 GTACATG 5370 0.0 21.592821 1 TAGACCC 110 0.0028769432 21.589994 4 >>END_MODULE