FastQCFastQC Report
Thu 26 May 2016
SRR937335_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937335_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366877
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53080.3883304788945896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37670.275591732101718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.19899376461817705No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25380.18567874066210785No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16050.11742095301918168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73000.030.7258781
GGTATCA49650.029.4792611
ATCAACG87400.025.3261053
TATCAAC87650.025.2006022
TCAACGC88100.025.0746384
GTACATG64850.024.9878881
CAACGCA88850.024.8122445
TACATGG65950.024.2010572
AACGCAG91200.024.0660516
GTATAGG5000.022.8100591
ACATGGG66650.022.6631833
GAGTACT44150.022.4328912-13
ACGCAGA101400.021.4125217
TACCTGG12050.021.2871042
CATGGGA37900.021.0585164
CGCAGAG102700.020.8176468
GTACTTT47250.020.75813314-15
CGGGTCG1150.003718074620.6574955
GTACCTG12600.020.3661231
GTGTAGC8400.019.8003981
Produced by FastQC (version 0.11.5)