##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937335_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1366877 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.446926826627415 31.0 31.0 34.0 30.0 34.0 2 31.566620844450526 33.0 31.0 34.0 30.0 34.0 3 31.599373608598288 33.0 31.0 34.0 30.0 34.0 4 35.23986210902663 37.0 35.0 37.0 33.0 37.0 5 35.24310819481197 37.0 35.0 37.0 33.0 37.0 6 35.26691721347276 37.0 35.0 37.0 33.0 37.0 7 35.188841424649034 37.0 35.0 37.0 32.0 37.0 8 35.16918859560882 37.0 35.0 37.0 32.0 37.0 9 36.72044082971621 39.0 37.0 39.0 33.0 39.0 10-11 36.58948208214784 39.0 37.0 39.0 32.0 39.0 12-13 36.42665543424902 39.0 37.0 39.0 32.0 39.0 14-15 37.46472323405837 40.0 37.0 41.0 32.0 41.0 16-17 37.37521444870314 40.0 37.0 41.0 32.0 41.0 18-19 37.280523412128524 40.0 37.0 41.0 32.0 41.0 20-21 37.12973881336799 39.0 36.5 41.0 31.0 41.0 22-23 37.208201250002745 39.0 37.0 41.0 31.5 41.0 24-25 37.16834287210919 39.5 37.0 41.0 31.0 41.0 26-27 37.07497821676712 39.0 36.5 41.0 31.0 41.0 28-29 36.92115128135158 39.0 36.0 41.0 31.0 41.0 30-31 36.659289753211155 39.0 36.0 40.5 30.0 41.0 32-33 36.41918036516819 39.0 35.5 40.0 30.0 41.0 34-35 36.3913453807475 39.0 35.0 40.5 30.0 41.0 36-37 36.23247190493366 39.0 35.0 40.5 30.0 41.0 38-39 36.06779871195433 39.0 35.0 40.0 29.5 41.0 40-41 35.81685842983677 38.5 35.0 40.0 28.0 41.0 42-43 35.5297363991054 38.0 35.0 40.0 27.0 41.0 44-45 35.262057961323514 38.0 34.5 40.0 26.5 41.0 46-47 34.85023341529633 38.0 34.0 40.0 24.5 41.0 48-49 34.48147894799605 38.0 33.5 40.0 24.0 41.0 50-51 33.51806161051799 37.5 32.0 39.5 22.5 40.5 52-53 33.63075682742485 37.5 33.0 39.5 23.0 40.0 54-55 33.90445519238381 38.0 33.0 40.0 22.5 41.0 56-57 33.57721140965866 38.0 33.0 40.0 20.0 41.0 58-59 33.25864141396775 37.5 32.5 40.0 18.5 41.0 60-61 32.87009548042728 37.0 32.0 40.0 16.5 41.0 62-63 32.43202497371746 36.5 31.0 39.0 13.0 41.0 64-65 31.944996513951146 36.0 31.0 39.0 8.0 40.5 66-67 31.45772040937114 35.5 30.5 39.0 7.5 40.0 68-69 30.900767954980587 35.0 29.5 38.0 4.0 40.0 70-71 30.376231365367914 35.0 29.0 38.0 2.0 40.0 72-73 29.824724170499614 34.0 28.5 37.0 2.0 39.0 74-75 29.212070654491953 34.0 28.0 36.5 2.0 39.0 76-77 28.616862014650916 34.0 26.5 36.0 2.0 38.5 78-79 27.99927681861645 33.5 26.0 35.5 2.0 37.5 80-81 27.329475512427233 33.0 25.5 35.0 2.0 37.0 82-83 26.689582164305932 32.5 24.5 35.0 2.0 36.5 84-85 26.012518317302874 32.0 22.5 35.0 2.0 36.0 86-87 25.31990552185749 31.5 20.0 34.5 2.0 35.5 88-89 24.647532660217415 31.0 17.5 34.0 2.0 35.0 90-91 23.975843108048494 31.0 9.0 34.0 2.0 35.0 92-93 23.182903436080935 30.5 2.0 34.0 2.0 35.0 94-95 22.283323590930273 29.5 2.0 34.0 2.0 35.0 96-97 21.30599241921548 29.0 2.0 34.0 2.0 35.0 98-99 20.00911128067851 28.5 2.0 34.0 2.0 35.0 100-101 17.0597972604704 22.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26633377935487346 1101 2 0.3507365289710904 1101 3 0.3438962133289749 1101 4 0.0999399658857385 1101 5 0.18209986627224595 1101 6 0.11231956029552492 1101 7 0.28649692143899586 1101 8 0.20354353502791867 1101 9 0.16572517447923474 1101 10-11 0.3384060369512838 1101 12-13 0.3879940980934009 1101 14-15 0.30676511034855025 1101 16-17 0.37149026109737804 1101 18-19 0.42543313877617095 1101 20-21 0.53320898867414 1101 22-23 0.5977804255381258 1101 24-25 0.46106392921495143 1101 26-27 0.326208376011472 1101 28-29 0.49548176296422497 1101 30-31 0.5371504304684009 1101 32-33 0.6126174294297755 1101 34-35 0.6555829107019022 1101 36-37 0.5810797560396992 1101 38-39 0.6416798554684178 1101 40-41 0.711730624406492 1101 42-43 0.6857023977346728 1101 44-45 0.7605050657272017 1101 46-47 0.9295134595732577 1101 48-49 0.6857606858117791 1101 50-51 0.5830806696875186 1101 52-53 0.7246462170217995 1101 54-55 0.8525302168527631 1101 56-57 0.8659858541912229 1101 58-59 1.0045142455455078 1101 60-61 1.0610133135340547 1101 62-63 1.2301866070373606 1101 64-65 0.8824442223305446 1101 66-67 1.0559247546617243 1101 68-69 1.104394798145652 1101 70-71 1.1580735392218138 1101 72-73 1.0837189456886591 1101 74-75 1.2249299506901394 1101 76-77 1.2103617769888224 1101 78-79 1.1647744401018123 1101 80-81 1.2153791749074934 1101 82-83 1.2240732304150832 1101 84-85 1.37188913775719 1101 86-87 1.462807212924126 1101 88-89 1.6588047127451553 1101 90-91 1.5698768084663435 1101 92-93 1.6399719111898072 1101 94-95 2.1671297401596874 1101 96-97 2.101498151326176 1101 98-99 2.294254536753872 1101 100-101 2.124681739516557 1102 1 0.18199521426985754 1102 2 0.22678533206531526 1102 3 0.10341670123229108 1102 4 0.2309072457018999 1102 5 0.1820260841361403 1102 6 0.08503173578374401 1102 7 0.10929017751246306 1102 8 0.0633195882978228 1102 9 0.017419491499936157 1102 10-11 0.08869159375913682 1102 12-13 0.24975768258112652 1102 14-15 0.4442656986115239 1102 16-17 0.542594225311845 1102 18-19 0.5223096817201665 1102 20-21 0.3091358318914814 1102 22-23 0.49038693703099057 1102 24-25 0.40016235937039113 1102 26-27 0.4399555034934437 1102 28-29 0.626239262220416 1102 30-31 0.5854960754478995 1102 32-33 0.5188092262919923 1102 34-35 0.6628935239231808 1102 36-37 0.8088007415137852 1102 38-39 0.4761139385080213 1102 40-41 0.6035632211149391 1102 42-43 0.6853927121741918 1102 44-45 0.556209908467558 1102 46-47 0.617348021678275 1102 48-49 0.5983005381358382 1102 50-51 0.5441117371515176 1102 52-53 0.42281825462914924 1102 54-55 0.7035483305665835 1102 56-57 0.6547653461074887 1102 58-59 0.5613470297004497 1102 60-61 0.7989511307473904 1102 62-63 0.7124763020259977 1102 64-65 0.5879137120213862 1102 66-67 0.7902829418067228 1102 68-69 0.8163240135583827 1102 70-71 1.1078147433941332 1102 72-73 0.9661317571390065 1102 74-75 0.9286051385952909 1102 76-77 1.1339438239206423 1102 78-79 1.2403803908504365 1102 80-81 1.2017304767833146 1102 82-83 1.166690270818183 1102 84-85 1.2501635690165678 1102 86-87 1.3527158905807397 1102 88-89 1.6077157681806646 1102 90-91 1.7345766826777727 1102 92-93 1.9599235579672047 1102 94-95 1.8100210108156922 1102 96-97 1.9737818719559002 1102 98-99 1.9534446800986025 1102 100-101 2.004606697101803 1103 1 0.29066351115032774 1103 2 0.3204839350087241 1103 3 0.27901057948140817 1103 4 0.28730260836978516 1103 5 0.19065853381265185 1103 6 0.2177578541221692 1103 7 0.19354628240613891 1103 8 0.16666576482848683 1103 9 0.2686079042398788 1103 10-11 0.280245475716022 1103 12-13 0.2189817915894423 1103 14-15 0.2676478782035545 1103 16-17 0.36309693928151177 1103 18-19 0.23513317812683 1103 20-21 0.13715005264955948 1103 22-23 -0.04382684027174122 1103 24-25 0.25348351668547764 1103 26-27 0.16478576247103405 1103 28-29 0.03524396501264704 1103 30-31 0.268801614210048 1103 32-33 0.2909912218082269 1103 34-35 0.4065021397207218 1103 36-37 0.28836270836865907 1103 38-39 0.25392771843571893 1103 40-41 0.39221237214322713 1103 42-43 0.2884359921359376 1103 44-45 0.3955611150910059 1103 46-47 0.38546706064509095 1103 48-49 0.4858986360192148 1103 50-51 0.3027017361917643 1103 52-53 0.45697276510153984 1103 54-55 0.6107141210138352 1103 56-57 0.6181591575840102 1103 58-59 0.5825106200973451 1103 60-61 0.48606246133489606 1103 62-63 0.5452780112075573 1103 64-65 0.528684410500464 1103 66-67 0.7548038484374686 1103 68-69 0.7871058763497203 1103 70-71 1.0827032438243833 1103 72-73 1.0840547028768377 1103 74-75 1.1309382009554803 1103 76-77 0.9405645646042622 1103 78-79 0.9363304771052121 1103 80-81 0.965786764276249 1103 82-83 0.8993267301316017 1103 84-85 1.2344923116656368 1103 86-87 1.5091364365246278 1103 88-89 1.6859965871375202 1103 90-91 1.4729706377071672 1103 92-93 1.7766159684496792 1103 94-95 1.8558619558693934 1103 96-97 1.6919939331865343 1103 98-99 1.7358933722498726 1103 100-101 1.5822802873920487 1104 1 0.024463095111308064 1104 2 0.1187362335610267 1104 3 -0.007098949331194149 1104 4 0.062271489832419036 1104 5 0.06258433878064551 1104 6 0.10341294680267055 1104 7 0.09767080718915366 1104 8 0.10757218980432981 1104 9 0.3107789021850067 1104 10-11 0.187333329148764 1104 12-13 0.15408286881826427 1104 14-15 0.34096990337687316 1104 16-17 0.21221992396063882 1104 18-19 0.2526939489382798 1104 20-21 0.22262961568200978 1104 22-23 0.3324948009304691 1104 24-25 0.23008279853292635 1104 26-27 0.24025697096734433 1104 28-29 0.2888830406127596 1104 30-31 0.30344044930078695 1104 32-33 0.25225592136138175 1104 34-35 0.4681193753819386 1104 36-37 0.4503700333559024 1104 38-39 0.5957786701355303 1104 40-41 0.4109533266700751 1104 42-43 0.3567134386858939 1104 44-45 0.5354339826800469 1104 46-47 0.5984178574906167 1104 48-49 0.7228531122362156 1104 50-51 0.41207147504921693 1104 52-53 0.3408531517731035 1104 54-55 0.6805886341933345 1104 56-57 0.5402704079373919 1104 58-59 0.7422731909315772 1104 60-61 0.6210886431009115 1104 62-63 0.49012161682281885 1104 64-65 0.4176958747806019 1104 66-67 0.6281100506256792 1104 68-69 0.6989020172291411 1104 70-71 0.5150304462784199 1104 72-73 0.7555737516411689 1104 74-75 0.807604150313491 1104 76-77 0.9755920122090878 1104 78-79 0.9965679484656071 1104 80-81 0.8979924462047109 1104 82-83 1.146391460908788 1104 84-85 0.8951416025849284 1104 86-87 0.9193302364461502 1104 88-89 0.97952772888193 1104 90-91 1.1225761179453144 1104 92-93 1.5813219351918768 1104 94-95 1.6408271663994825 1104 96-97 1.8163037345764224 1104 98-99 1.862008681726362 1104 100-101 1.6346961407572813 1105 1 0.20618883161497337 1105 2 0.15772056663925582 1105 3 0.20962269670721412 1105 4 0.2147394038710715 1105 5 0.30147982519414285 1105 6 0.22382480827224782 1105 7 0.09411166258021098 1105 8 0.0938801270662708 1105 9 0.1867867840277384 1105 10-11 0.23764429247532348 1105 12-13 0.25395355733593306 1105 14-15 0.263858475584577 1105 16-17 0.2149055563113791 1105 18-19 0.31536207585705966 1105 20-21 0.3971512951478644 1105 22-23 0.43712318436100617 1105 24-25 0.4109675705664628 1105 26-27 0.4126489694894957 1105 28-29 0.28417328771204353 1105 30-31 0.4252738574060686 1105 32-33 0.3481139954119996 1105 34-35 0.5225779825125372 1105 36-37 0.5151951109867738 1105 38-39 0.4213858623170239 1105 40-41 0.475443250050823 1105 42-43 0.5482738852063562 1105 44-45 0.3522567907624534 1105 46-47 0.41282157321647617 1105 48-49 0.6885635776695835 1105 50-51 0.6867475365909286 1105 52-53 0.3802117844141293 1105 54-55 0.7309664524359292 1105 56-57 0.7813773845997432 1105 58-59 0.920454737215401 1105 60-61 1.0033527376126514 1105 62-63 0.9857566945416565 1105 64-65 1.016334585536324 1105 66-67 0.9842607029009542 1105 68-69 0.8554819484389533 1105 70-71 1.0987838220118462 1105 72-73 1.159397237069438 1105 74-75 1.2426946243676262 1105 76-77 1.2264360555240366 1105 78-79 1.2322605565990337 1105 80-81 1.2475323788219903 1105 82-83 1.4223333675054342 1105 84-85 1.3121640957454943 1105 86-87 1.2234022286346793 1105 88-89 1.3400472619531207 1105 90-91 1.4268904592044755 1105 92-93 1.735481454540217 1105 94-95 1.8108735250057322 1105 96-97 1.7548161247276397 1105 98-99 1.7591013725487663 1105 100-101 1.7960425635794373 1106 1 0.09664862277900355 1106 2 -0.4859399477877808 1106 3 -0.0770404970643348 1106 4 0.09567303186710774 1106 5 0.10599701869188749 1106 6 0.04515235502742598 1106 7 0.03137108606776451 1106 8 0.05314768992952423 1106 9 -0.025237018934653577 1106 10-11 -0.20680331273828756 1106 12-13 0.0039766704604531355 1106 14-15 -0.07501726648006013 1106 16-17 -0.01988867465274069 1106 18-19 0.1236258179430294 1106 20-21 0.03563891451382517 1106 22-23 0.1530731071098259 1106 24-25 0.006243425707616268 1106 26-27 0.06322430849680671 1106 28-29 0.08860114009385711 1106 30-31 0.031504444603882575 1106 32-33 0.030344091900403214 1106 34-35 0.02355362305722508 1106 36-37 -0.08927087805049183 1106 38-39 0.14143792018517587 1106 40-41 0.16418546937304512 1106 42-43 0.04508471850561335 1106 44-45 0.05222843273659805 1106 46-47 0.34024058393918466 1106 48-49 0.33088619777337414 1106 50-51 0.1794051192070114 1106 52-53 0.2621001622461563 1106 54-55 0.37876599408488687 1106 56-57 0.14103538147413985 1106 58-59 0.057369330664435836 1106 60-61 0.30093599375407365 1106 62-63 0.3047640391814568 1106 64-65 0.3401654614704164 1106 66-67 0.586916312011784 1106 68-69 0.4668383154824802 1106 70-71 0.5151103108971 1106 72-73 0.48283607885298707 1106 74-75 0.47754397124406367 1106 76-77 0.39014809443942156 1106 78-79 0.5487450148007262 1106 80-81 0.6011067346325412 1106 82-83 0.6496158330002011 1106 84-85 0.5581824155635751 1106 86-87 0.36011947155895285 1106 88-89 0.5303820644514765 1106 90-91 0.7217321431911685 1106 92-93 0.9201188718441244 1106 94-95 0.9439828980889011 1106 96-97 0.9173916201930226 1106 98-99 1.2468603062158756 1106 100-101 1.0316604218462047 1107 1 0.13472421770875087 1107 2 0.05253622725480511 1107 3 0.00390946046507068 1107 4 -0.02210890142355737 1107 5 -0.13566613531204297 1107 6 -0.024357398643111594 1107 7 0.07273781078820463 1107 8 0.09221654145723335 1107 9 0.2462320266923328 1107 10-11 0.010295631459996457 1107 12-13 0.13142168856394676 1107 14-15 0.23255064778123113 1107 16-17 0.37305378656609633 1107 18-19 0.4207968574575247 1107 20-21 0.38261799421742637 1107 22-23 0.36333942915620554 1107 24-25 0.30944478913665563 1107 26-27 0.372959907414149 1107 28-29 0.3734188351740997 1107 30-31 0.27104133757707416 1107 32-33 0.14334455529876067 1107 34-35 0.41043366843202733 1107 36-37 0.25310130275315856 1107 38-39 0.21742409835046317 1107 40-41 0.40148070693070537 1107 42-43 0.4539668541802726 1107 44-45 0.4571352659960368 1107 46-47 0.5024042901063908 1107 48-49 0.4876017868036726 1107 50-51 0.5487534117797992 1107 52-53 0.6254056127758787 1107 54-55 0.6579405168255477 1107 56-57 0.6007069786143617 1107 58-59 0.8944479363178957 1107 60-61 0.6555399793441126 1107 62-63 0.5982850200200787 1107 64-65 0.43580396341003436 1107 66-67 0.5892567468414995 1107 68-69 0.7142056392522669 1107 70-71 0.8418762546683993 1107 72-73 0.9956425795398935 1107 74-75 0.8503256450680645 1107 76-77 0.9033312769937965 1107 78-79 0.749182102328394 1107 80-81 0.8152343303233955 1107 82-83 0.790522846520691 1107 84-85 1.0701140128676734 1107 86-87 1.1182248459784532 1107 88-89 1.1297036274058279 1107 90-91 1.192233059518177 1107 92-93 1.0947672489939428 1107 94-95 1.3417767031174535 1107 96-97 1.3305920550045265 1107 98-99 1.5340264022076155 1107 100-101 1.5019992853093846 1108 1 0.01830025210636066 1108 2 0.14745471150551026 1108 3 0.1361706122046229 1108 4 0.15789214025183185 1108 5 0.09576948868406276 1108 6 -0.012389757544930546 1108 7 0.09758083867812672 1108 8 0.1695181557750658 1108 9 0.27759110614891114 1108 10-11 0.17507379645579135 1108 12-13 0.21664538370276176 1108 14-15 0.3137001830730952 1108 16-17 0.2906698194383992 1108 18-19 0.2889777202065673 1108 20-21 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2316 58-59 0.03794361284453629 2316 60-61 -0.08943709138108602 2316 62-63 0.09158538324390264 2316 64-65 0.3800673972795998 2316 66-67 0.3082494755142591 2316 68-69 0.4750893377504575 2316 70-71 0.43111888297848466 2316 72-73 0.40799582053499606 2316 74-75 0.4589074416764234 2316 76-77 0.553515971719996 2316 78-79 0.38883590932788437 2316 80-81 0.7194311343373521 2316 82-83 0.6563324684582632 2316 84-85 0.9567809170250818 2316 86-87 1.1718133768195926 2316 88-89 1.4298616729300626 2316 90-91 1.6294128751796855 2316 92-93 1.7043218442343537 2316 94-95 1.8386623773559663 2316 96-97 2.343680649557129 2316 98-99 2.720398863511466 2316 100-101 3.174833746495402 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6811.0 3 1387.0 4 1454.0 5 1670.0 6 1922.0 7 2205.0 8 2752.0 9 3448.0 10 4255.0 11 5327.0 12 6595.0 13 8172.0 14 9286.0 15 11041.0 16 13350.0 17 16313.0 18 15035.0 19 12639.0 20 13302.0 21 14416.0 22 15824.0 23 17831.0 24 19977.0 25 22340.0 26 26014.0 27 30150.0 28 34612.0 29 40808.0 30 47877.0 31 56381.0 32 68751.0 33 85829.0 34 108238.0 35 138241.0 36 176400.0 37 199250.0 38 117241.0 39 9733.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.050894988023025 19.32290263449413 14.931467328147102 22.694735049335744 2 14.858532791335868 20.15144622338318 40.74446384808418 24.245557137196776 3 19.178761648933556 24.695042090611665 29.625142986279922 26.50105327417486 4 12.160282222561369 16.303078151230906 37.82467627757364 33.711963348634086 5 13.355159998209645 37.82174091377024 34.21425107072705 14.608848017293058 6 31.659801496841727 36.259098550392984 17.722039835587985 14.3590601171773 7 28.42675448890975 32.793539490670106 20.586052106560263 18.193653913859876 8 26.40696228760879 34.79266069850258 19.743058433485288 19.057318580403347 9 26.219549767900425 16.83044700441899 20.42667003127916 36.52333319640143 10-11 24.611140964953627 26.990714334184933 27.331879563282097 21.066265137579347 12-13 25.621039929431653 24.17768991375184 28.071969439946304 22.129300716870198 14-15 22.60579746606116 25.365426811400997 26.50473476965009 25.52404095288775 16-17 21.736118521973864 29.05257452455266 26.93412516186241 22.27718179161106 18-19 21.650905120146728 27.506199566076212 29.485587269319858 21.357308044457206 20-21 22.380553903381166 26.398454591525045 29.64548452794709 21.5755069771467 22-23 22.04959811082754 26.402468538238143 29.5201531285752 22.02778022235912 24-25 21.856413024505656 26.63822072544484 29.610963372794863 21.89440287725464 26-27 21.736410618202193 26.84145372888926 29.503777914509055 21.918357738399497 28-29 21.588610241100834 26.977400922740642 29.657126301042304 21.776862535116223 30-31 21.716691914653246 26.61873828536216 29.612330309688257 22.052239490296337 32-33 21.536780064147116 26.779899103077337 29.541856085265415 22.141464747510128 34-35 21.932567819289883 26.798370583957215 29.35292437049374 21.91613722625917 36-37 21.902783782604374 26.52868216883702 29.433294719089627 22.135239329468977 38-39 21.725541958843213 26.834667033319583 29.498510472523947 21.94128053531326 40-41 21.860966652880805 26.853465090972023 29.395783286256083 21.88978496989109 42-43 21.670541470176236 26.74578844306239 29.266342014359996 22.317328072401377 44-45 21.7918593582472 26.842652198847443 29.095658627522592 22.269829815382767 46-47 21.99957239301849 27.094403226048968 28.706144154894947 22.199880226037596 48-49 21.99273896623975 27.25168881933567 28.446909696256412 22.30866251816817 50-51 21.930220325591527 27.38366936415676 28.494577509373727 22.191532800877987 52-53 22.109937020254993 27.50549655752061 28.203677676657506 22.180888745566893 54-55 21.968109920868944 27.29269541438912 28.351883800285776 22.38731086445616 56-57 21.983196543246994 27.55719243312807 28.185369663215905 22.274241360409032 58-59 22.13789290849249 27.204653717027433 28.3592480088335 22.298205365646577 60-61 22.041428036182626 27.263632091000613 28.644419987696672 22.05051988512009 62-63 21.909460445911318 27.325936308544147 28.662145054084466 22.10245819146007 64-65 21.811136487779486 27.387592153354912 28.485550573907815 22.315720784957787 66-67 21.768536394545603 27.46097900231483 28.261379642576678 22.50910496056289 68-69 21.741956629813593 27.34529555050103 28.48274739440121 22.43000042528417 70-71 21.8556744874271 27.34072881333562 28.345087204485097 22.458509494752185 72-73 21.871625776037142 27.17894436584113 28.414810893136117 22.534618964985615 74-75 21.78534854379718 27.662188665630488 28.138661468914872 22.41380132165746 76-77 21.909694566800585 27.374797030596127 28.10655031954388 22.60895808305941 78-79 21.911516964150167 27.385289106728088 28.11118208894362 22.59201184017813 80-81 21.90391752017867 27.55343181309013 28.01855047976706 22.524100186964137 82-83 21.956525883700433 27.385144241556763 28.20549013971728 22.452839735025528 84-85 21.94206944686791 27.346372155562626 28.057234060959928 22.654324336609534 86-87 21.89057243315711 27.25395074450734 28.204761606245672 22.65071521608988 88-89 22.03846588184222 27.132614016845324 28.00043557903413 22.828484522278327 90-91 21.825353323108928 27.176263931913237 28.261778995190035 22.736603749787808 92-93 22.00080078377078 26.985313097654558 28.26447717326262 22.74940894531204 94-95 21.810440375307657 26.906520294089503 28.250586899429468 23.03245243117337 96-97 21.742851359913644 26.877654706310285 28.35846015300495 23.021033780771127 98-99 21.763154999816656 26.886106120054272 28.057606981775514 23.29313189835356 100-101 22.237408035804627 26.01727027831588 28.73146968206088 23.013852003818613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2261.0 1 1246.5 2 236.5 3 249.0 4 223.0 5 192.0 6 325.0 7 685.0 8 1173.5 9 1459.0 10 1370.5 11 1282.0 12 1373.5 13 1491.5 14 1635.0 15 1760.0 16 1819.5 17 1888.0 18 2031.5 19 2174.5 20 2369.0 21 2797.0 22 3309.0 23 3892.0 24 4674.5 25 5745.0 26 7345.5 27 9373.5 28 11018.0 29 13034.0 30 15864.5 31 18569.0 32 21641.0 33 24712.5 34 28085.0 35 31372.0 36 34965.0 37 38675.5 38 40944.0 39 42426.5 40 43338.5 41 44625.5 42 46030.5 43 47846.0 44 50208.0 45 51903.5 46 52293.5 47 53745.5 48 57308.5 49 56870.0 50 52472.0 51 48622.0 52 45842.5 53 44016.5 54 41942.5 55 38703.0 56 34802.5 57 30819.5 58 27430.0 59 24559.5 60 21042.0 61 17024.0 62 13330.0 63 10045.0 64 7500.0 65 5704.0 66 4132.0 67 3007.0 68 2099.5 69 1408.0 70 1038.0 71 727.5 72 536.5 73 410.0 74 321.0 75 216.0 76 130.5 77 88.0 78 49.0 79 29.5 80 22.5 81 16.0 82 12.5 83 10.0 84 8.0 85 6.5 86 4.5 87 3.5 88 4.5 89 3.5 90 1.5 91 1.5 92 3.0 93 2.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3423863302989223 2 0.2938816001732416 3 0.29146733758780047 4 0.29124785917094226 5 0.29395475964552775 6 0.2540828472496062 7 0.2573018640301944 8 0.2583992561144858 9 0.2684221038176807 10-11 0.2746772386981418 12-13 0.26772708883096286 14-15 0.2564605300989043 16-17 0.2524367591231691 18-19 0.2394875325285304 20-21 0.24537687004756095 22-23 0.24266996957297546 24-25 0.24545002951984707 26-27 0.2409873017103953 28-29 0.2594966481987772 30-31 0.2730311505717047 32-33 0.2637033178552276 34-35 0.2609232579083561 36-37 0.24804719078600343 38-39 0.2333421368564984 40-41 0.23125709189634475 42-43 0.24632794318728019 44-45 0.24135309907182576 46-47 0.2542657459303215 48-49 0.2613622147420726 50-51 0.27493329685114315 52-53 0.29106496049022695 54-55 0.26110615658907127 56-57 0.23187894741077655 58-59 0.21786890846798943 60-61 0.22079528735943324 62-63 0.22902572799161888 64-65 0.2181615463571338 66-67 0.22386798519544918 68-69 0.22555065305802938 70-71 0.2246727393905962 72-73 0.2295378442976215 74-75 0.23487848577450643 76-77 0.24662058107642457 78-79 0.2475716542161438 80-81 0.2375122267768058 82-83 0.2284770319494731 84-85 0.21208931015738794 86-87 0.21863708292699344 88-89 0.23228132450835007 90-91 0.23019627954819635 92-93 0.23506138445522165 94-95 0.22990364165905197 96-97 0.23857303912495417 98-99 0.24340156429583643 100-101 0.22174636049915245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1366877.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.7875132767368 #Duplication Level Percentage of deduplicated Percentage of total 1 80.59876860932894 48.993987169282214 2 11.263253537383425 13.693303478858954 3 3.3538933717685286 6.11624513595417 4 1.4876936575681596 3.617327918445666 5 0.8000831834030256 2.4317533566802636 6 0.5044148055796225 1.839727301469235 7 0.36480814852355725 1.5523046120286255 8 0.25357055686450036 1.2331138873592287 9 0.18672445935458074 1.0215463996897272 >10 1.0853514635934036 12.066957964000892 >50 0.07116197333464702 2.956205990727701 >100 0.028828786824317016 2.89574639466524 >500 7.237232367031292E-4 0.32628390762666015 >1k 6.031026972526076E-4 0.8663021053053909 >5k 1.2062053945052152E-4 0.38919437790613753 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5308 0.3883304788945896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3767 0.275591732101718 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2720 0.19899376461817705 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2538 0.18567874066210785 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1605 0.11742095301918168 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 7.31594722860945E-5 10-11 0.0 0.0 0.0 0.0 7.31594722860945E-5 12-13 0.0 0.0 0.0 0.0 7.31594722860945E-5 14-15 0.0 0.0 0.0 7.31594722860945E-5 7.31594722860945E-5 16-17 0.0 0.0 0.0 1.46318944572189E-4 7.31594722860945E-5 18-19 0.0 0.0 0.0 1.46318944572189E-4 1.46318944572189E-4 20-21 0.0 0.0 0.0 1.46318944572189E-4 1.46318944572189E-4 22-23 0.0 0.0 0.0 1.8289868071523626E-4 1.46318944572189E-4 24-25 7.31594722860945E-5 0.0 0.0 3.6579736143047253E-4 1.46318944572189E-4 26-27 7.31594722860945E-5 0.0 0.0 5.121163060026615E-4 1.46318944572189E-4 28-29 7.31594722860945E-5 0.0 0.0 0.0016460881264371264 1.46318944572189E-4 30-31 7.31594722860945E-5 0.0 0.0 0.0034384951974464418 1.8289868071523626E-4 32-33 7.31594722860945E-5 0.0 0.0 0.005962496991316702 2.1947841685828352E-4 34-35 7.31594722860945E-5 0.0 0.0 0.009400992188763144 2.1947841685828352E-4 36-37 7.31594722860945E-5 0.0 0.0 0.012583429233208255 2.1947841685828352E-4 38-39 7.31594722860945E-5 0.0 0.0 0.01543664865236594 2.1947841685828352E-4 40-41 7.31594722860945E-5 0.0 0.0 0.019643318308816375 2.1947841685828352E-4 42-43 7.31594722860945E-5 0.0 0.0 0.02611793160613574 2.1947841685828352E-4 44-45 7.31594722860945E-5 0.0 0.0 0.03240964622273987 2.1947841685828352E-4 46-47 1.46318944572189E-4 0.0 0.0 0.041481420786215584 2.1947841685828352E-4 48-49 1.46318944572189E-4 0.0 0.0 0.04828525170882238 2.92637889144378E-4 50-51 1.46318944572189E-4 0.0 0.0 0.059076273871021316 2.92637889144378E-4 52-53 1.46318944572189E-4 0.0 0.0 0.09082748484318633 2.92637889144378E-4 54-55 1.46318944572189E-4 0.0 0.0 0.13552792240999006 2.92637889144378E-4 56-57 1.46318944572189E-4 0.0 0.0 0.16760835100744254 2.92637889144378E-4 58-59 1.46318944572189E-4 0.0 0.0 0.2185273437185643 2.92637889144378E-4 60-61 1.46318944572189E-4 0.0 0.0 0.27796941495101607 2.92637889144378E-4 62-63 1.46318944572189E-4 0.0 0.0 0.3184266031252263 2.92637889144378E-4 64-65 1.46318944572189E-4 0.0 0.0 0.352226279321402 2.92637889144378E-4 66-67 1.46318944572189E-4 0.0 0.0 0.4460533025283182 2.92637889144378E-4 68-69 1.46318944572189E-4 0.0 0.0 0.5427335451543921 2.92637889144378E-4 70-71 1.46318944572189E-4 0.0 0.0 0.591384594224645 2.92637889144378E-4 72-73 1.46318944572189E-4 0.0 0.0 0.6930396809661732 2.92637889144378E-4 74-75 1.46318944572189E-4 0.0 0.0 0.8391025673853609 2.92637889144378E-4 76-77 1.46318944572189E-4 0.0 0.0 0.9763497373940742 2.92637889144378E-4 78-79 1.46318944572189E-4 0.0 0.0 1.1300943684033018 2.92637889144378E-4 80-81 2.1947841685828352E-4 0.0 0.0 1.3332216432056434 2.92637889144378E-4 82-83 2.1947841685828352E-4 0.0 0.0 1.5495542027556248 2.92637889144378E-4 84-85 2.5605815300133077E-4 0.0 0.0 1.742439151437913 2.92637889144378E-4 86-87 2.92637889144378E-4 0.0 0.0 2.033760170081141 2.92637889144378E-4 88-89 2.92637889144378E-4 0.0 0.0 2.3743906730451974 2.92637889144378E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7300 0.0 30.725878 1 GGTATCA 4965 0.0 29.479261 1 ATCAACG 8740 0.0 25.326105 3 TATCAAC 8765 0.0 25.200602 2 TCAACGC 8810 0.0 25.074638 4 GTACATG 6485 0.0 24.987888 1 CAACGCA 8885 0.0 24.812244 5 TACATGG 6595 0.0 24.201057 2 AACGCAG 9120 0.0 24.066051 6 GTATAGG 500 0.0 22.810059 1 ACATGGG 6665 0.0 22.663183 3 GAGTACT 4415 0.0 22.43289 12-13 ACGCAGA 10140 0.0 21.412521 7 TACCTGG 1205 0.0 21.287104 2 CATGGGA 3790 0.0 21.058516 4 CGCAGAG 10270 0.0 20.817646 8 GTACTTT 4725 0.0 20.758133 14-15 CGGGTCG 115 0.0037180746 20.657495 5 GTACCTG 1260 0.0 20.366123 1 GTGTAGC 840 0.0 19.800398 1 >>END_MODULE