FastQCFastQC Report
Thu 26 May 2016
SRR937335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937335_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366877
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61000.4462727809451765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.3334608746800187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33470.2448647537415583No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22590.16526724789428748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81700.029.1363411
ACCGCGC700.00838326327.1487338
GGTATCA55750.027.1020181
TATCAAC98750.023.905382
TCAACGC98500.023.7249494
ATCAACG99200.023.5575333
GTACATG62100.023.4890881
CAACGCA99950.023.3807645
AACGCAG102300.022.9365316
TACCTGG10200.022.8177222
TACATGG62900.022.8051362
GAGTACT51550.022.1192412-13
ACATGGG62850.021.9163953
GTACCTG10900.021.7953011
GTACTTT53700.021.05130614-15
AGTACTT55450.020.3493112-13
ACGCAGA115250.020.0759287
CGCAGAG115200.019.9196768
AGAGTAC91200.019.79595210-11
TAGACTG5050.019.751695
Produced by FastQC (version 0.11.5)