##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937335_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1366877 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.150122505536345 33.0 31.0 34.0 31.0 34.0 2 32.345816046359694 34.0 31.0 34.0 31.0 34.0 3 32.42056820035746 34.0 31.0 34.0 31.0 34.0 4 36.068649190819656 37.0 35.0 37.0 35.0 37.0 5 35.99091944629985 37.0 35.0 37.0 35.0 37.0 6 36.02841294425175 37.0 35.0 37.0 35.0 37.0 7 35.9657291768023 37.0 35.0 37.0 35.0 37.0 8 35.9374713306318 37.0 35.0 37.0 35.0 37.0 9 37.61637952793119 39.0 37.0 39.0 35.0 39.0 10-11 37.5323284392085 39.0 37.0 39.0 35.0 39.0 12-13 37.41936948240405 39.0 37.0 39.0 34.0 39.0 14-15 38.621507275343724 40.0 38.0 41.0 34.0 41.0 16-17 38.57699339443125 40.0 38.0 41.0 34.0 41.0 18-19 38.55574422570575 40.0 38.0 41.0 34.0 41.0 20-21 38.448647903212944 40.0 38.0 41.0 34.0 41.0 22-23 38.39239668236425 40.0 38.0 41.0 34.0 41.0 24-25 38.43210032797391 40.0 38.0 41.0 34.0 41.0 26-27 38.2719205897824 40.0 38.0 41.0 34.0 41.0 28-29 38.11946685766166 40.0 38.0 41.0 33.5 41.0 30-31 38.17893709529095 40.0 38.0 41.0 34.0 41.0 32-33 38.10107859009992 40.0 37.0 41.0 33.5 41.0 34-35 37.97682673715338 40.0 37.0 41.0 33.0 41.0 36-37 37.812875628165514 40.0 37.0 41.0 33.0 41.0 38-39 37.71191116684237 40.0 37.0 41.0 33.0 41.0 40-41 37.53215066169085 40.0 37.0 41.0 33.0 41.0 42-43 37.325563675444094 40.0 36.5 41.0 32.0 41.0 44-45 37.02807897126077 39.5 36.0 41.0 31.0 41.0 46-47 36.69080795126409 39.0 36.0 41.0 31.0 41.0 48-49 36.371159584951684 39.0 36.0 41.0 30.0 41.0 50-51 36.12266575558738 39.0 35.0 40.0 30.0 41.0 52-53 35.87909592450528 39.0 35.0 40.0 29.0 41.0 54-55 35.65900443126924 38.5 35.0 40.0 28.5 41.0 56-57 35.34845198214616 38.0 34.5 40.0 28.0 41.0 58-59 35.07366537003695 38.0 34.0 40.0 27.5 41.0 60-61 34.71262154531827 38.0 34.0 40.0 26.5 41.0 62-63 34.3275031330544 37.0 34.0 40.0 26.0 41.0 64-65 33.89017812136717 36.5 33.0 39.0 26.0 41.0 66-67 33.43057422138203 36.0 32.5 39.0 24.5 40.0 68-69 32.95645731108212 35.5 32.0 39.0 23.0 40.0 70-71 32.50893935591864 35.0 31.5 38.0 22.5 40.0 72-73 32.00665531719386 35.0 31.0 37.5 21.5 39.5 74-75 31.503782710514553 34.5 31.0 37.0 20.0 39.0 76-77 30.200772271389454 33.5 29.5 35.5 19.5 37.5 78-79 30.867797175605414 34.0 30.5 36.0 20.0 38.0 80-81 30.678323653115825 34.0 30.0 35.5 18.0 37.0 82-83 30.263916943514303 34.0 30.0 35.0 17.0 37.0 84-85 29.80257257968347 34.0 29.5 35.0 11.5 36.0 86-87 29.406025560456428 34.0 29.0 35.0 7.0 36.0 88-89 28.923031845586692 34.0 29.0 35.0 2.0 35.5 90-91 28.325504050474184 33.0 28.5 35.0 2.0 35.0 92-93 27.79011278995842 33.0 27.0 35.0 2.0 35.0 94-95 27.24818692537807 33.0 26.0 34.5 2.0 35.0 96-97 26.50095473111333 32.0 25.0 34.0 2.0 35.0 98-99 25.450085486843363 32.0 22.0 34.0 2.0 35.0 100-101 22.44876751895013 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20542631954367607 1101 2 0.2188180246466942 1101 3 0.15477941612326163 1101 4 0.14242277965867345 1101 5 0.08365239769874222 1101 6 0.14882079443559348 1101 7 0.07273672036287593 1101 8 0.07684316670410851 1101 9 0.23525477809376127 1101 10-11 0.21581944457751234 1101 12-13 0.2320890426975808 1101 14-15 0.30124312919995333 1101 16-17 0.224334308687844 1101 18-19 0.24579592589372368 1101 20-21 0.271325927908876 1101 22-23 0.29812968550088925 1101 24-25 0.2190072765763631 1101 26-27 0.1645035510389974 1101 28-29 0.08221198401981411 1101 30-31 0.4227995323793081 1101 32-33 0.381089735832596 1101 34-35 0.4403196821541684 1101 36-37 0.4131459429160671 1101 38-39 0.45090767282485444 1101 40-41 0.3022832926490153 1101 42-43 0.2961601030323209 1101 44-45 0.27058573542778674 1101 46-47 0.21699060736612807 1101 48-49 0.22182281049575892 1101 50-51 0.1695258899389458 1101 52-53 0.2995885327285066 1101 54-55 0.1355899008051793 1101 56-57 0.05578276070355059 1101 58-59 0.3057018888912566 1101 60-61 0.2550843946748884 1101 62-63 0.4325050079035222 1101 64-65 0.4213217465033452 1101 66-67 0.2782477514676529 1101 68-69 0.31779202057188627 1101 70-71 0.2374495787559141 1101 72-73 0.30089343205468566 1101 74-75 0.2878597847192701 1101 76-77 0.4180883863048095 1101 78-79 0.5495570906277614 1101 80-81 0.41141253778947373 1101 82-83 0.11621673359245932 1101 84-85 0.11403181494828729 1101 86-87 0.49422004244012285 1101 88-89 0.7449600719792642 1101 90-91 0.8762768880822804 1101 92-93 0.7783776606703725 1101 94-95 0.8083237071430212 1101 96-97 1.0665095566769658 1101 98-99 1.56933330915572 1101 100-101 2.433129548440167 1102 1 0.2749490328757531 1102 2 0.2424733751277941 1102 3 0.2668812019700155 1102 4 0.1588095926771942 1102 5 0.20574189184044656 1102 6 0.23400326762875068 1102 7 0.24961443584460596 1102 8 0.16269526263524625 1102 9 0.24089332858187618 1102 10-11 0.2494060699899947 1102 12-13 0.19849184849262258 1102 14-15 0.2567453223240932 1102 16-17 0.33455445065265366 1102 18-19 0.28643293293932004 1102 20-21 0.3412504547659694 1102 22-23 0.29082385814789546 1102 24-25 0.18985735047814245 1102 26-27 0.2626934160057246 1102 28-29 0.2522183891068792 1102 30-31 0.35292326302745636 1102 32-33 0.3936440654082318 1102 34-35 0.4453891131383543 1102 36-37 0.5110209376316703 1102 38-39 0.429181426086231 1102 40-41 0.4668393914006188 1102 42-43 0.610689560450453 1102 44-45 0.6715196577419746 1102 46-47 0.4770251772880556 1102 48-49 0.4853065955751745 1102 50-51 0.4088677612613054 1102 52-53 0.5347756721432475 1102 54-55 0.43698573917365024 1102 56-57 0.7134954666255027 1102 58-59 0.5924075045493353 1102 60-61 0.7491461291852275 1102 62-63 0.6759342101551127 1102 64-65 0.6649302194045674 1102 66-67 0.8023872722905665 1102 68-69 0.8134530327351683 1102 70-71 0.6913983587982031 1102 72-73 0.8777899719516782 1102 74-75 0.726988924196224 1102 76-77 0.6853928895675772 1102 78-79 0.5885379878344885 1102 80-81 0.7845477441521247 1102 82-83 0.7155149258902398 1102 84-85 0.7427481413796038 1102 86-87 0.8211347287543731 1102 88-89 0.8251482922025879 1102 90-91 1.1922883693803037 1102 92-93 1.3016192329557263 1102 94-95 1.318714824252421 1102 96-97 1.5858368869361783 1102 98-99 2.049625502367604 1102 100-101 2.8015253493193306 1103 1 0.21051840049662474 1103 2 0.1876357662429342 1103 3 0.1742731907351569 1103 4 0.05319322994614595 1103 5 0.10024816428083483 1103 6 0.08240773893170683 1103 7 0.13338172268704085 1103 8 0.05379851160596871 1103 9 0.07177081399485985 1103 10-11 0.08419209007262651 1103 12-13 0.1717680450468606 1103 14-15 0.18489505699736952 1103 16-17 0.23705579799893428 1103 18-19 0.15422093897345945 1103 20-21 0.10083199403014476 1103 22-23 0.22331155566639893 1103 24-25 0.1425468341225482 1103 26-27 0.19138803638219315 1103 28-29 0.11824418104708201 1103 30-31 0.06160382342506665 1103 32-33 0.07398031546257045 1103 34-35 0.15205521653087573 1103 36-37 0.112236379327463 1103 38-39 0.1594234234235259 1103 40-41 0.29771318545392234 1103 42-43 0.26471543934268027 1103 44-45 0.2982803337693838 1103 46-47 0.40391779891631074 1103 48-49 0.19889912524755715 1103 50-51 0.05596741894922275 1103 52-53 0.24657030457815665 1103 54-55 0.14398139895497053 1103 56-57 0.31999570492327933 1103 58-59 0.2548230236816593 1103 60-61 0.4378080359119494 1103 62-63 0.3758787577297653 1103 64-65 0.5105762196523003 1103 66-67 0.38234909542850204 1103 68-69 0.4162256129873825 1103 70-71 0.3559870634016562 1103 72-73 0.45323439114904573 1103 74-75 0.47648156731638736 1103 76-77 0.3911929589651386 1103 78-79 0.4530486817034678 1103 80-81 0.6380222979692789 1103 82-83 0.5624467497582444 1103 84-85 0.5152032975118139 1103 86-87 0.7872037506178877 1103 88-89 0.8426127300536272 1103 90-91 1.233185968602001 1103 92-93 1.2957952927655967 1103 94-95 1.0826652927679916 1103 96-97 1.3674179044881818 1103 98-99 1.7624159816127296 1103 100-101 2.3384545318144134 1104 1 0.08471807391021002 1104 2 0.10374103339821517 1104 3 0.1114095257942509 1104 4 0.029073937296871577 1104 5 -0.011200081899495729 1104 6 5.877158934453064E-4 1104 7 -0.04207070920028144 1104 8 0.08170266229142698 1104 9 0.07292694677290967 1104 10-11 0.06318844867632833 1104 12-13 0.1351483831946041 1104 14-15 0.11512370968073071 1104 16-17 -0.010507462295500147 1104 18-19 -0.05020865296230426 1104 20-21 0.1467939682323518 1104 22-23 -0.04264982781102589 1104 24-25 -0.14852161822073384 1104 26-27 0.1250884629698703 1104 28-29 0.020384503498419804 1104 30-31 0.07444828981072504 1104 32-33 0.18831066563578247 1104 34-35 0.20597826197027302 1104 36-37 0.2265095557811776 1104 38-39 0.2952173364266457 1104 40-41 0.3074677393090326 1104 42-43 0.3722134245492441 1104 44-45 0.4522647723909685 1104 46-47 0.34989559610362164 1104 48-49 0.3994141106693121 1104 50-51 0.5105860428136566 1104 52-53 0.409431226868314 1104 54-55 0.5002274695803379 1104 56-57 0.48062305463656685 1104 58-59 0.35077592603906993 1104 60-61 0.4003079831686662 1104 62-63 0.5197229836060373 1104 64-65 0.20445567804206632 1104 66-67 0.46174356033733943 1104 68-69 0.5584090583474506 1104 70-71 0.6754430058819452 1104 72-73 0.8142995101167969 1104 74-75 0.5910391355556506 1104 76-77 0.6635630733547693 1104 78-79 0.5988148728366944 1104 80-81 0.5367938014060307 1104 82-83 0.49824657000913675 1104 84-85 0.6058711118723288 1104 86-87 0.6392497680780238 1104 88-89 0.7367772131483505 1104 90-91 0.9225214876170078 1104 92-93 0.8169803710577241 1104 94-95 0.8572196120209803 1104 96-97 1.1590116799368175 1104 98-99 1.3350620488625289 1104 100-101 2.109406273819584 1105 1 0.03799968265060727 1105 2 0.12301108327550025 1105 3 0.0947793654304192 1105 4 0.06527587433009785 1105 5 0.0066554587016653954 1105 6 0.002340018429535462 1105 7 -0.012536670371652292 1105 8 0.07262974644945075 1105 9 0.13458301341670165 1105 10-11 0.04090948774246783 1105 12-13 0.08992455117638087 1105 14-15 0.16969139588486826 1105 16-17 0.1663919650393666 1105 18-19 0.18978202253625653 1105 20-21 0.37049887415164307 1105 22-23 0.1963221898879297 1105 24-25 0.14312811479943832 1105 26-27 0.21150152079057705 1105 28-29 0.21055507150131092 1105 30-31 0.196036709835532 1105 32-33 0.24975429198717336 1105 34-35 0.1451891100600733 1105 36-37 0.1960109588547425 1105 38-39 0.14288928990969652 1105 40-41 0.13966761791457571 1105 42-43 0.20036858073464714 1105 44-45 0.2106044833314229 1105 46-47 0.16745820661215305 1105 48-49 0.26671238035852696 1105 50-51 0.2603199426544336 1105 52-53 0.033056962492302944 1105 54-55 0.25597611268943865 1105 56-57 0.4213759641449215 1105 58-59 0.33070317733438515 1105 60-61 0.3174874974149162 1105 62-63 0.5060106884591775 1105 64-65 0.48218991215743046 1105 66-67 0.5373335989548522 1105 68-69 0.43025346232707307 1105 70-71 0.5337110237216507 1105 72-73 0.6070761003401444 1105 74-75 0.5425970310332637 1105 76-77 0.4577352599503577 1105 78-79 0.5232265935590767 1105 80-81 0.5250409710679733 1105 82-83 0.5379636681539104 1105 84-85 0.692045779137171 1105 86-87 0.9139557383979593 1105 88-89 1.0028632976494443 1105 90-91 1.2464696222768872 1105 92-93 1.4217437838252565 1105 94-95 1.5305752889709865 1105 96-97 1.6672062453358798 1105 98-99 1.7595419440058713 1105 100-101 2.2867983163252354 1106 1 -0.06753928874422854 1106 2 0.061981766943944194 1106 3 0.021650328412079034 1106 4 0.13302399816666366 1106 5 -0.008910341696946489 1106 6 0.08329551499723209 1106 7 0.08706045008862162 1106 8 -0.025174328927782597 1106 9 0.01068866528682122 1106 10-11 -0.15660416233549057 1106 12-13 -0.2679866268640154 1106 14-15 -0.09009123406867303 1106 16-17 -0.07301555668252746 1106 18-19 -0.017603598611195537 1106 20-21 0.15500232422996163 1106 22-23 0.12181214367945614 1106 24-25 0.1829342490226864 1106 26-27 0.18132955914413884 1106 28-29 0.11023522141658759 1106 30-31 0.3216094263921718 1106 32-33 0.29121212564735544 1106 34-35 -0.26729126600825026 1106 36-37 0.2961454594601278 1106 38-39 0.3393846290592393 1106 40-41 0.28708801542476436 1106 42-43 0.15290000124949898 1106 44-45 0.341364762893555 1106 46-47 0.26977023023732727 1106 48-49 0.39036048481562347 1106 50-51 0.4837407384215382 1106 52-53 0.3555601672159199 1106 54-55 0.19466543851515894 1106 56-57 0.24112115083674013 1106 58-59 0.3249675665878513 1106 60-61 0.4746874134964969 1106 62-63 0.5136580212935087 1106 64-65 0.5505798696826147 1106 66-67 0.4989302535967681 1106 68-69 0.541370697428448 1106 70-71 0.7003646522155123 1106 72-73 0.9069657796188721 1106 74-75 0.7968791818339618 1106 76-77 0.5061870361939818 1106 78-79 0.7224910628195111 1106 80-81 0.7110847331023571 1106 82-83 0.6122479169151376 1106 84-85 0.7334213834120327 1106 86-87 0.9506976056895091 1106 88-89 0.8457069409179248 1106 90-91 0.7668860674427727 1106 92-93 1.0338179911784309 1106 94-95 0.8729171107474656 1106 96-97 1.3261312005764232 1106 98-99 1.749803762368714 1106 100-101 2.136391880920197 1107 1 -0.026106104141440767 1107 2 -0.03515708960011921 1107 3 -0.009185691387500583 1107 4 -0.05788975238299798 1107 5 -0.07297357144693706 1107 6 -0.1413252312288904 1107 7 -0.09126689123385745 1107 8 -0.13750518625116115 1107 9 -0.11233631543525746 1107 10-11 -0.1248936943577732 1107 12-13 -0.05387496333675301 1107 14-15 0.041944067964173826 1107 16-17 -0.08958409639504339 1107 18-19 -0.043090280688055316 1107 20-21 -0.045798699434307366 1107 22-23 -0.03187542386153552 1107 24-25 -0.08418622635665685 1107 26-27 -0.03041754370571681 1107 28-29 0.011799259154877006 1107 30-31 0.04569462431971516 1107 32-33 0.012360115417990869 1107 34-35 -0.1367756647784617 1107 36-37 -0.2055255968969405 1107 38-39 -0.17593733265439937 1107 40-41 -0.21947649117434054 1107 42-43 -0.19697167655299097 1107 44-45 -0.1593740342918224 1107 46-47 -0.2056468965653977 1107 48-49 -0.11561293851470822 1107 50-51 -0.3493967051672229 1107 52-53 -0.2846289885341875 1107 54-55 -0.3176590004223243 1107 56-57 -0.2945907938249377 1107 58-59 -0.16698486998620865 1107 60-61 -0.10627255853572848 1107 62-63 -0.2592185171844861 1107 64-65 0.10456896479944788 1107 66-67 0.07415532954767201 1107 68-69 0.13181144480168427 1107 70-71 0.18126995416642444 1107 72-73 0.0731917322939708 1107 74-75 0.13453089017157893 1107 76-77 0.05279384951545296 1107 78-79 0.01729412762153615 1107 80-81 0.07753041307344333 1107 82-83 0.1282752182706446 1107 84-85 0.20105451029487398 1107 86-87 0.11172694180990916 1107 88-89 0.3938304962120931 1107 90-91 0.5624495516521826 1107 92-93 0.6814092301272012 1107 94-95 0.7622211210095955 1107 96-97 0.9718130835214538 1107 98-99 1.085389296692508 1107 100-101 1.7218563308007973 1108 1 -0.0053986205408520505 1108 2 0.03513739761191914 1108 3 0.05898963746104613 1108 4 -0.012666159551585565 1108 5 -0.0338721466768277 1108 6 0.015877070501680635 1108 7 0.013606568458421009 1108 8 0.022029724290312913 1108 9 0.004987490288563379 1108 10-11 0.05556494043361937 1108 12-13 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1.6314229638672195 2316 98-99 2.050614485874444 2316 100-101 2.517747078663721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 12.0 5 29.0 6 40.0 7 137.0 8 312.0 9 601.0 10 1038.0 11 1646.0 12 2505.0 13 3603.0 14 4786.0 15 6626.0 16 9297.0 17 13069.0 18 11617.0 19 7472.0 20 6738.0 21 7196.0 22 7845.0 23 8635.0 24 10099.0 25 11793.0 26 14783.0 27 18475.0 28 23007.0 29 28800.0 30 36089.0 31 44933.0 32 57517.0 33 75280.0 34 101793.0 35 142884.0 36 212081.0 37 290572.0 38 192549.0 39 13010.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7907800561956 20.128317447352302 15.28157453090429 21.799327965547803 2 15.681001289801497 21.135625224508132 39.683380435840235 23.499993049850133 3 19.797831114284605 25.110891470117647 29.769686665296145 25.3215907503016 4 13.064306444544755 17.109586305132062 37.25982659741879 32.566280652904396 5 13.565787404023075 38.1551213909201 33.95829141868436 14.320799786372465 6 31.790570768254934 36.99908623819115 17.151872480113425 14.058470513440493 7 28.71377600179094 33.130559662647045 20.258077354436427 17.89758698112559 8 26.767075603730255 35.339317290436526 19.182559952358552 18.711047153474674 9 26.34114115608061 17.19079331936963 20.285731634960573 36.18233388958919 10-11 24.908422630565884 27.473649787069355 26.960070291620973 20.657857290743788 12-13 26.033083549534602 24.518833288702954 27.827371153556307 21.620712008206137 14-15 22.77337317110464 25.71965143901024 26.403802244093654 25.103173145791462 16-17 21.91203012414431 29.455356992618942 26.784707036551204 21.84790584668555 18-19 21.834993199827053 27.695103509679363 29.51286033783581 20.957042952657773 20-21 22.55017653419408 26.584235269673723 29.77304418695505 21.092544009177143 22-23 22.3198667313403 26.5738775575899 29.627146246551412 21.47910946451839 24-25 22.194068008338718 26.842366519579492 29.677699484920545 21.28586598716124 26-27 22.061141556336512 26.99740167956194 29.61284184118969 21.32861492291186 28-29 21.800879559210752 27.172431503466804 29.684720969314927 21.341967968007516 30-31 21.980742154620973 26.808006458544202 29.6834779275155 21.52777345931933 32-33 21.764193979911088 27.012997179329325 29.593009155565603 21.629799685193984 34-35 22.275596121670056 26.89605575337064 29.470757061535164 21.35759106342414 36-37 22.206387260887407 26.657921671079404 29.459380763594677 21.676310304438513 38-39 22.060165684438708 26.98043823303585 29.612060498560407 21.347335583965034 40-41 22.253940917873372 27.042411277678973 29.40820571273055 21.295442091717103 42-43 21.988565566903652 26.99819059681039 29.236348444374205 21.776895391911754 44-45 22.169624626063648 27.052873082215882 29.05996662464874 21.717535667071726 46-47 22.32907569591119 27.465529085645603 28.584869011622843 21.620526206820365 48-49 22.398796672999836 27.507486043001673 28.34750310379061 21.746214180207875 50-51 22.284009460982958 27.65193942103057 28.330493526484098 21.733557591502382 52-53 22.59844887286859 27.794454073043877 27.940297481046212 21.66679957304132 54-55 22.35947345664606 27.631601087735035 28.151801515425305 21.857123940193596 56-57 22.377397527356155 27.89662127609141 28.023918757869215 21.702062438683217 58-59 22.593364289544706 27.52753173840806 28.08844541242555 21.79065855962168 60-61 22.385957185613627 27.584742445735788 28.524878244348244 21.504422124302334 62-63 22.284594736761246 27.605922112962617 28.538595645401895 21.57088750487425 64-65 22.216995384368893 27.703846066617555 28.271563571557646 21.807594977455906 66-67 22.21176448210044 27.812999999268406 28.06013269665083 21.915102821980327 68-69 22.222189706901208 27.70494345870184 28.190283397847793 21.882583436549154 70-71 22.292349640823574 27.68277613859916 28.115002300865406 21.90987191971187 72-73 22.383262831833804 27.44792131535167 28.075338326722143 22.093477526092382 74-75 22.212166859198014 28.060461914276118 27.804696399163937 21.922674827361934 76-77 22.336574541820514 27.750924187033654 27.90605884801632 22.006442423129513 78-79 22.342098081978115 27.767129010145027 27.845775442852577 22.044997465024284 80-81 22.289130624042983 27.981888641040854 27.774188899220633 21.95479183569553 82-83 22.35259302179687 27.877659222289164 27.828459243212873 21.941288512701092 84-85 22.206545738670865 27.84733633092736 27.78299243383077 22.163125496571006 86-87 22.174160513345385 27.79291772412587 27.912643200522062 22.120278562006675 88-89 22.345097620341843 27.70117574587911 27.752241057534803 22.20148557624424 90-91 22.24497709878982 27.684343766919824 27.9043563516104 22.166322782679952 92-93 22.266480350878474 27.580394409063143 27.898789557048847 22.254335683009536 94-95 22.152139512187272 27.56861078209671 27.931079387538162 22.34817031817786 96-97 22.155834065537718 27.452177481953388 27.976401680619396 22.415586771889497 98-99 21.98846491646813 27.574204928254154 27.706916550800052 22.730413604477665 100-101 21.983668025042704 27.463012331478875 28.12518863793261 22.42813100554582 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 164.0 1 131.0 2 121.5 3 162.0 4 158.5 5 154.0 6 295.0 7 706.0 8 1225.5 9 1576.0 10 1587.0 11 1446.0 12 1477.0 13 1665.0 14 1853.0 15 1953.0 16 1998.0 17 2077.5 18 2192.5 19 2378.5 20 2607.0 21 2838.5 22 3267.0 23 3929.5 24 4643.5 25 5664.0 26 7332.5 27 9208.0 28 10869.0 29 12994.5 30 15936.5 31 18709.5 32 21548.5 33 24860.0 34 28481.5 35 31443.0 36 35262.0 37 39404.5 38 41512.5 39 42961.5 40 43762.5 41 45107.5 42 46759.5 43 48749.0 44 50889.0 45 51978.5 46 52291.5 47 53960.0 48 58237.5 49 57688.0 50 52788.5 51 49017.5 52 45813.5 53 43642.5 54 41968.5 55 38790.5 56 34240.0 57 30211.0 58 27140.5 59 24050.5 60 20310.5 61 16173.5 62 12516.5 63 9532.0 64 7001.0 65 5135.0 66 3686.0 67 2763.5 68 1966.0 69 1249.0 70 828.5 71 561.5 72 403.0 73 329.5 74 238.5 75 151.0 76 95.0 77 54.5 78 31.5 79 18.5 80 12.0 81 8.0 82 4.0 83 2.0 84 2.0 85 1.5 86 1.0 87 0.5 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.042286174981362626 2 0.0 3 0.0 4 0.0 5 8.779136674331341E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006950149867178979 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.1947841685828352E-4 22-23 0.001024232612005323 24-25 3.657973614304725E-5 26-27 0.0023776828492980714 28-29 0.004096930448021292 30-31 4.0237709757351983E-4 32-33 2.5605815300133077E-4 34-35 0.0 36-37 0.0 38-39 1.0973920842914176E-4 40-41 0.0 42-43 0.009364412452620097 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.1947841685828352E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.755365698596143E-4 84-85 2.1947841685828352E-4 86-87 0.0 88-89 0.0 90-91 0.010022847703194946 92-93 0.0016095083902940793 94-95 0.0 96-97 0.0 98-99 2.5605815300133077E-4 100-101 0.012546849497065209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1366877.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.82629659198607 #Duplication Level Percentage of deduplicated Percentage of total 1 79.34635567312452 46.6765225232044 2 11.823087608641433 13.910169165979527 3 3.6563476454615653 6.45268173105996 4 1.6196455562283594 3.8111099945832687 5 0.875462033001212 2.57500946041762 6 0.55422015646936 1.9561631581034102 7 0.3704128592659786 1.5253011704466235 8 0.26486886323886544 1.2465003445497356 9 0.19727092392511233 1.0444246091814402 >10 1.1775571859970215 12.601863328575286 >50 0.08012795205046927 3.214420468407108 >100 0.03314815720973697 3.3609187629178034 >500 8.723081420614685E-4 0.34879305758758483 >1k 4.984617954636963E-4 0.8289516383355212 >5k 1.2461544886592407E-4 0.4471705866507498 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6100 0.4462727809451765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4558 0.3334608746800187 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3347 0.2448647537415583 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2259 0.16526724789428748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.31594722860945E-5 0.0 16-17 0.0 0.0 0.0 7.31594722860945E-5 0.0 18-19 0.0 0.0 0.0 7.31594722860945E-5 0.0 20-21 0.0 0.0 0.0 7.31594722860945E-5 0.0 22-23 0.0 0.0 0.0 1.0973920842914176E-4 0.0 24-25 0.0 0.0 0.0 2.92637889144378E-4 0.0 26-27 0.0 0.0 0.0 4.3895683371656703E-4 0.0 28-29 0.0 0.0 0.0 0.0015729286541510319 0.0 30-31 0.0 0.0 0.0 0.0034384951974464418 0.0 32-33 0.0 0.0 0.0 0.0058527577828875604 0.0 34-35 0.0 0.0 0.0 0.009291252980334001 0.0 36-37 0.0 0.0 0.0 0.012437110288636068 7.31594722860945E-5 38-39 2.1947841685828352E-4 0.0 0.0 0.015217170235507657 7.31594722860945E-5 40-41 2.1947841685828352E-4 0.0 0.0 0.019496999364244184 1.46318944572189E-4 42-43 2.1947841685828352E-4 0.0 0.0 0.026008192397706596 1.46318944572189E-4 44-45 2.92637889144378E-4 0.0 0.0 0.03251938543116901 1.46318944572189E-4 46-47 2.92637889144378E-4 0.0 0.0 0.04159115999464473 1.8289868071523626E-4 48-49 2.92637889144378E-4 0.0 0.0 0.048760788278681994 2.1947841685828352E-4 50-51 2.92637889144378E-4 0.0 0.0 0.059807868593882256 2.1947841685828352E-4 52-53 2.92637889144378E-4 0.0 0.0 0.09163223903833337 2.5605815300133077E-4 54-55 2.92637889144378E-4 0.0 0.0 0.13677163343885368 2.92637889144378E-4 56-57 2.92637889144378E-4 0.0 0.0 0.16907154045316442 2.92637889144378E-4 58-59 2.92637889144378E-4 0.0 0.0 0.2208684468317193 2.92637889144378E-4 60-61 2.92637889144378E-4 0.0 0.0 0.2813347506761764 2.92637889144378E-4 62-63 2.92637889144378E-4 0.0 0.0 0.32201141726724497 2.92637889144378E-4 64-65 3.6579736143047253E-4 0.0 0.0 0.356103731352565 2.92637889144378E-4 66-67 4.3895683371656703E-4 0.0 0.0 0.45025997218476865 2.92637889144378E-4 68-69 4.3895683371656703E-4 0.0 0.0 0.5486228826734227 2.92637889144378E-4 70-71 4.3895683371656703E-4 0.0 0.0 0.5980786859388225 2.92637889144378E-4 72-73 4.755365698596143E-4 0.0 0.0 0.7015627594875032 2.92637889144378E-4 74-75 5.121163060026615E-4 0.0 0.0 0.8501130679644182 2.92637889144378E-4 76-77 5.121163060026615E-4 0.0 0.0 0.9888234274188534 3.6579736143047253E-4 78-79 5.121163060026615E-4 0.0 0.0 1.1453481183749525 3.6579736143047253E-4 80-81 5.85275778288756E-4 0.0 0.0 1.3519870478470266 3.6579736143047253E-4 82-83 5.85275778288756E-4 0.0 0.0 1.5717946823305975 3.6579736143047253E-4 84-85 6.218555144318033E-4 0.0 0.0 1.7693984169753387 3.6579736143047253E-4 86-87 6.584352505748505E-4 0.0 0.0 2.0682914410001776 3.6579736143047253E-4 88-89 6.584352505748505E-4 0.0 0.0 2.4181766172084247 3.6579736143047253E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8170 0.0 29.136341 1 ACCGCGC 70 0.008383263 27.148733 8 GGTATCA 5575 0.0 27.102018 1 TATCAAC 9875 0.0 23.90538 2 TCAACGC 9850 0.0 23.724949 4 ATCAACG 9920 0.0 23.557533 3 GTACATG 6210 0.0 23.489088 1 CAACGCA 9995 0.0 23.380764 5 AACGCAG 10230 0.0 22.936531 6 TACCTGG 1020 0.0 22.817722 2 TACATGG 6290 0.0 22.805136 2 GAGTACT 5155 0.0 22.11924 12-13 ACATGGG 6285 0.0 21.916395 3 GTACCTG 1090 0.0 21.795301 1 GTACTTT 5370 0.0 21.051306 14-15 AGTACTT 5545 0.0 20.34931 12-13 ACGCAGA 11525 0.0 20.075928 7 CGCAGAG 11520 0.0 19.919676 8 AGAGTAC 9120 0.0 19.795952 10-11 TAGACTG 505 0.0 19.75169 5 >>END_MODULE