FastQCFastQC Report
Thu 26 May 2016
SRR937334_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937334_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330136
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52690.39612490752825275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36570.27493429243325496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25980.195318373459556No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20370.1531422350797212No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15790.11870966577853692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79050.029.4771041
GTACATG59500.025.4955461
TATCAAC90950.025.2438012
ATCAACG91200.025.1215323
TCAACGC91750.024.9765954
CAACGCA92250.024.7381465
TACATGG58450.024.6415752
AACGCAG94900.024.1794016
GGTATCA54750.023.6251961
GTACACG1852.5143345E-723.1345671
GTAGCAC3950.022.8639093
ACATGGG61550.022.4728893
GAGTACT45150.022.46364212-13
CGCAGAG104050.021.8704668
ACGCAGA104800.021.8046157
TAAGGTG4400.021.6107395
GTACCTG11050.021.5178221
TACCTGG11050.021.5088942
GTACTTT47850.021.19450414-15
GCAGAGT108600.020.0792489
Produced by FastQC (version 0.11.5)