##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937334_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1330136 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.482271737626828 31.0 31.0 34.0 30.0 34.0 2 31.591805649948576 33.0 31.0 34.0 30.0 34.0 3 31.630903907570353 33.0 31.0 34.0 30.0 34.0 4 35.2557625686396 37.0 35.0 37.0 33.0 37.0 5 35.252071216777836 37.0 35.0 37.0 33.0 37.0 6 35.291225859611345 37.0 35.0 37.0 33.0 37.0 7 35.21945124408331 37.0 35.0 37.0 32.0 37.0 8 35.21426004558932 37.0 35.0 37.0 32.0 37.0 9 36.798401065755684 39.0 37.0 39.0 33.0 39.0 10-11 36.66298521354207 39.0 37.0 39.0 32.0 39.0 12-13 36.51711704667793 39.0 37.0 39.0 32.0 39.0 14-15 37.63884745619997 40.0 37.0 41.0 32.0 41.0 16-17 37.595335364203365 40.0 37.0 41.0 32.0 41.0 18-19 37.56548089819387 40.0 37.0 41.0 32.0 41.0 20-21 37.49785773785538 40.0 37.0 41.0 32.0 41.0 22-23 37.33701666596498 40.0 37.0 41.0 32.0 41.0 24-25 37.16466323744339 39.5 37.0 41.0 31.0 41.0 26-27 37.000898780275094 39.0 36.0 41.0 31.0 41.0 28-29 37.06720703747587 39.0 37.0 41.0 31.0 41.0 30-31 36.92282180168043 39.0 36.0 41.0 30.5 41.0 32-33 36.7331859298598 39.0 36.0 41.0 30.0 41.0 34-35 36.60358978330035 39.0 36.0 41.0 30.0 41.0 36-37 36.609273788544925 39.0 36.0 41.0 30.0 41.0 38-39 36.5258638966241 39.0 36.0 41.0 30.0 41.0 40-41 36.3551452633415 39.0 35.0 41.0 30.0 41.0 42-43 36.11237384748627 39.0 35.0 41.0 29.5 41.0 44-45 35.891017535049045 39.0 35.0 41.0 28.5 41.0 46-47 35.55680359000884 39.0 35.0 40.0 27.0 41.0 48-49 35.24456484148989 39.0 35.0 40.0 26.0 41.0 50-51 34.37850716017009 38.0 34.0 39.5 24.5 40.5 52-53 34.48272920964473 38.0 34.0 39.5 24.0 40.5 54-55 34.8011109390318 38.0 34.0 40.0 24.5 41.0 56-57 34.59434336037819 38.0 34.0 40.0 24.0 41.0 58-59 34.34172332753944 38.0 34.0 40.0 23.0 41.0 60-61 34.03482726578335 38.0 33.5 40.0 22.5 41.0 62-63 33.69464363042576 37.0 33.0 40.0 22.0 41.0 64-65 33.31633043538405 37.0 33.0 40.0 21.0 41.0 66-67 32.92641729868224 36.0 32.0 39.0 19.5 41.0 68-69 32.44295921620045 36.0 31.5 39.0 18.0 40.0 70-71 31.986722410339997 35.0 31.0 38.5 15.5 40.0 72-73 31.58159128089158 35.0 31.0 38.0 12.5 40.0 74-75 31.112979424660335 35.0 31.0 37.0 8.0 39.0 76-77 30.65685125430783 35.0 30.0 36.5 7.0 39.0 78-79 30.168138821894907 34.0 30.0 36.0 4.0 38.5 80-81 29.665866121960462 34.0 29.0 35.5 2.0 37.5 82-83 29.167269361929907 34.0 29.0 35.0 2.0 37.0 84-85 28.711010753787583 34.0 29.0 35.0 2.0 36.5 86-87 28.26270020509181 34.0 27.5 35.0 2.0 36.0 88-89 27.83015420979509 33.5 27.0 35.0 2.0 36.0 90-91 27.37756590303548 33.0 26.0 35.0 2.0 35.0 92-93 26.882139871411646 33.0 25.5 35.0 2.0 35.0 94-95 26.310449457799805 32.5 24.5 35.0 2.0 35.0 96-97 25.634518951445564 32.0 22.5 34.5 2.0 35.0 98-99 24.8218415259793 32.0 17.5 34.0 2.0 35.0 100-101 22.867934932969263 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12859753835363463 1101 2 0.135172901977338 1101 3 0.1809187303252564 1101 4 0.16956009100103842 1101 5 0.1447823135532147 1101 6 0.13446022664032142 1101 7 0.3071371997700041 1101 8 0.17176334805525784 1101 9 0.07545709683377311 1101 10-11 0.29642967946774945 1101 12-13 0.0922916193379919 1101 14-15 0.10323286889237693 1101 16-17 0.35801533888272985 1101 18-19 0.3438527408515526 1101 20-21 0.3005968923127682 1101 22-23 0.30255444162892076 1101 24-25 0.34590122152356884 1101 26-27 0.3247506332519592 1101 28-29 0.2360670986942779 1101 30-31 0.2163387857702972 1101 32-33 0.2847469584988005 1101 34-35 0.25061213214295464 1101 36-37 0.12581773891732695 1101 38-39 0.21960981726684992 1101 40-41 0.24398723090239827 1101 42-43 0.34959147689934156 1101 44-45 0.30177330411630265 1101 46-47 0.37465775987234196 1101 48-49 0.47638862310321173 1101 50-51 0.23889067861406232 1101 52-53 0.26369006120526706 1101 54-55 0.23052040538178176 1101 56-57 0.14059705059225536 1101 58-59 0.4132194248379619 1101 60-61 0.4178839321468715 1101 62-63 0.5355249193784957 1101 64-65 0.43016683225482666 1101 66-67 0.4496991015820129 1101 68-69 0.5151566529309335 1101 70-71 0.41938361411997604 1101 72-73 0.5057641094830316 1101 74-75 0.3354509110609811 1101 76-77 0.3869699939544802 1101 78-79 0.2685983422595797 1101 80-81 0.37329542982050157 1101 82-83 0.4713714384008796 1101 84-85 0.5066588810666595 1101 86-87 0.6889188085010005 1101 88-89 0.5619205888117342 1101 90-91 0.7198094458006743 1101 92-93 1.09471719118147 1101 94-95 1.196995841050903 1101 96-97 0.9628671588523012 1101 98-99 0.8219179252835396 1101 100-101 1.5741806290374996 1102 1 0.32988452972231386 1102 2 0.27597669359262866 1102 3 0.29382178211317367 1102 4 0.0939612932205236 1102 5 0.11946095103780152 1102 6 0.08499274821861746 1102 7 0.1780488828772846 1102 8 0.17260721365328635 1102 9 0.21825379843673431 1102 10-11 0.28950535764285235 1102 12-13 0.3189620108299849 1102 14-15 0.5187854274620349 1102 16-17 0.4919537636669489 1102 18-19 0.5269426761166542 1102 20-21 0.5307336833115315 1102 22-23 0.5952679126523535 1102 24-25 0.41182600574058625 1102 26-27 0.442375093794503 1102 28-29 0.515242884760859 1102 30-31 0.5149177006901482 1102 32-33 0.4509615084371461 1102 34-35 0.5319773448433267 1102 36-37 0.3608319960073416 1102 38-39 0.513526278425914 1102 40-41 0.7247975730725642 1102 42-43 0.7001328609930582 1102 44-45 0.3725162141039604 1102 46-47 0.5836404972336169 1102 48-49 0.5677542211303361 1102 50-51 0.4348717205376076 1102 52-53 0.5904390593556954 1102 54-55 0.6307943727060774 1102 56-57 0.5420470351791877 1102 58-59 0.6292093292769323 1102 60-61 0.7002625542183836 1102 62-63 0.5149280482317593 1102 64-65 0.6251113452018089 1102 66-67 0.6136040029383594 1102 68-69 0.5221179661245188 1102 70-71 0.6950748903221999 1102 72-73 0.5303880305681083 1102 74-75 0.5558659079783652 1102 76-77 0.6506598829803742 1102 78-79 0.7534180093372633 1102 80-81 0.6289450907329517 1102 82-83 0.6656185099175254 1102 84-85 0.8904323859988388 1102 86-87 0.7379597302026042 1102 88-89 0.7364146547796757 1102 90-91 0.8075371739141097 1102 92-93 0.7939534735489175 1102 94-95 0.8876038866735918 1102 96-97 1.1830763912814035 1102 98-99 1.350664842669481 1102 100-101 1.5277148460535344 1103 1 -0.04461619382243498 1103 2 -0.15660445388131095 1103 3 -0.037289189699542646 1103 4 -0.2669522307596566 1103 5 -0.026804403743746263 1103 6 -0.16117949747623328 1103 7 -0.10956111083135767 1103 8 0.01335703993375148 1103 9 0.04392385132850052 1103 10-11 0.011147983869491895 1103 12-13 -0.08240905487155459 1103 14-15 0.0351311174974569 1103 16-17 -0.1430209676888765 1103 18-19 -0.05444139275043369 1103 20-21 -0.27075055809021364 1103 22-23 -0.33849678279761264 1103 24-25 -0.3516530252216299 1103 26-27 -0.1565735685459302 1103 28-29 -0.34146486813771304 1103 30-31 -0.3155939482656649 1103 32-33 -0.22042973089983775 1103 34-35 -0.28294006097546287 1103 36-37 -0.16708620559600007 1103 38-39 -0.216674280687279 1103 40-41 0.048159774299797675 1103 42-43 -0.06638903302006582 1103 44-45 0.09945929295696487 1103 46-47 0.0942826823769849 1103 48-49 0.09935359520488873 1103 50-51 0.07034410390853907 1103 52-53 -0.03269923668685948 1103 54-55 -0.07621529672609029 1103 56-57 0.027157225731748724 1103 58-59 0.016650725209942152 1103 60-61 0.023453329233042552 1103 62-63 -0.1440897349922423 1103 64-65 -0.04097389930127804 1103 66-67 -0.15670627659530112 1103 68-69 -0.21413697695127354 1103 70-71 -0.004565936406986992 1103 72-73 -0.03840204054796814 1103 74-75 -0.049188929298601636 1103 76-77 0.0053875389017825626 1103 78-79 -0.04430230870136498 1103 80-81 -0.016981616056746418 1103 82-83 -0.18669389017940574 1103 84-85 -0.06767330275231487 1103 86-87 -0.010904308361986637 1103 88-89 0.27584669699524156 1103 90-91 0.33453995572007855 1103 92-93 0.2607149438163141 1103 94-95 0.3349139284656566 1103 96-97 0.431737470383613 1103 98-99 0.40044628858174036 1103 100-101 0.6384234994652758 1104 1 0.20338066138202393 1104 2 0.08089253920131156 1104 3 0.059469597833142984 1104 4 0.04897846639536141 1104 5 0.15267072049327624 1104 6 0.04588964304410581 1104 7 -0.031614771838825106 1104 8 0.07851610830762468 1104 9 0.09282894225964355 1104 10-11 0.14561146180214024 1104 12-13 0.10485569662506578 1104 14-15 0.2106204871889048 1104 16-17 0.14222176632752337 1104 18-19 0.06097123454240716 1104 20-21 0.1826522945209632 1104 22-23 0.012757517338698676 1104 24-25 0.2206804013342918 1104 26-27 0.20733442662735513 1104 28-29 0.11431926746399768 1104 30-31 0.1189649687355967 1104 32-33 0.06092992379848283 1104 34-35 0.0642881968117166 1104 36-37 0.27865981304982057 1104 38-39 0.189684738402498 1104 40-41 -0.05732093155816642 1104 42-43 0.11062755734098317 1104 44-45 0.1917023261983175 1104 46-47 0.16991012580294296 1104 48-49 0.19904635180983377 1104 50-51 0.2953082653648451 1104 52-53 0.2907533498488064 1104 54-55 0.405379435350234 1104 56-57 0.29440324144399455 1104 58-59 0.3122720667937955 1104 60-61 0.2355130725254142 1104 62-63 0.07936265597155767 1104 64-65 0.09593970291728482 1104 66-67 0.20123892808042854 1104 68-69 0.41206319867225716 1104 70-71 0.17521760465625746 1104 72-73 0.3677199454451774 1104 74-75 0.14434976752785644 1104 76-77 0.11557409490085035 1104 78-79 0.4685076482532686 1104 80-81 0.5836207452779121 1104 82-83 0.49342388319583463 1104 84-85 0.6792367596155522 1104 86-87 0.5639355671760775 1104 88-89 0.36658245789690724 1104 90-91 0.3726653211949902 1104 92-93 0.5513871438628577 1104 94-95 0.7854244688111542 1104 96-97 0.9101946430794321 1104 98-99 1.1775843290690275 1104 100-101 1.303403815157374 1105 1 0.21349337168696536 1105 2 0.21493852697733828 1105 3 0.1607885219919254 1105 4 0.28224238266770385 1105 5 0.2245218968865501 1105 6 0.19433001830698515 1105 7 0.24737157477000693 1105 8 0.2695498063885964 1105 9 0.23136855516710852 1105 10-11 0.24883853363442654 1105 12-13 0.07595047350466189 1105 14-15 0.18269901472569927 1105 16-17 0.09887471388272928 1105 18-19 0.15726419918487977 1105 20-21 0.08261512147943506 1105 22-23 0.18504142079558505 1105 24-25 0.20517205485689516 1105 26-27 0.2255449040852895 1105 28-29 0.41188741119427874 1105 30-31 0.22705493160362522 1105 32-33 -8.65020667866645E-4 1105 34-35 -0.03042953452371222 1105 36-37 -0.15437757358267135 1105 38-39 -0.025858932733150652 1105 40-41 -0.011338289930925782 1105 42-43 -0.016619460600658442 1105 44-45 -0.2460391958837036 1105 46-47 -0.03786828179433144 1105 48-49 0.024995393936542598 1105 50-51 -0.06039317555261192 1105 52-53 -0.09196098046140122 1105 54-55 -0.04266969878488425 1105 56-57 0.05253715475893017 1105 58-59 0.1226334873379642 1105 60-61 -0.02565773451980391 1105 62-63 -0.0510896639548335 1105 64-65 0.10275797808815668 1105 66-67 0.14949076824868257 1105 68-69 0.1397530070976032 1105 70-71 0.1682898641199806 1105 72-73 0.33956619281636335 1105 74-75 0.0914925777276423 1105 76-77 0.024040306454480742 1105 78-79 0.010603550592914956 1105 80-81 -0.00843634101283186 1105 82-83 -0.058706686599119706 1105 84-85 0.18010940189999403 1105 86-87 0.40850214183433664 1105 88-89 0.4857226721450658 1105 90-91 0.4579474666340069 1105 92-93 0.6529463578481334 1105 94-95 0.48109740355090125 1105 96-97 0.6234530963523035 1105 98-99 0.771279904450207 1105 100-101 1.0680998998708375 1106 1 -0.13565419175017368 1106 2 -0.2108629976766423 1106 3 -0.1603507333425611 1106 4 -0.027851241178893815 1106 5 -0.25185047191391163 1106 6 -0.12019158996867674 1106 7 -0.16681392479746648 1106 8 -0.28237808793090124 1106 9 -0.17054852600359993 1106 10-11 -0.5582307876602712 1106 12-13 -0.6814486488280949 1106 14-15 -0.630333307578212 1106 16-17 -0.6083092718439147 1106 18-19 -0.4360510221242251 1106 20-21 -0.4390127530159589 1106 22-23 -0.3108646760181344 1106 24-25 -0.26868355356294416 1106 26-27 -0.35258392557157237 1106 28-29 -0.1411139134164543 1106 30-31 -0.3019916640566933 1106 32-33 -0.03065096537663692 1106 34-35 -0.2267873055733105 1106 36-37 -0.3030414738854361 1106 38-39 -0.6028512553975105 1106 40-41 -0.3806440407238938 1106 42-43 -0.3823559711975477 1106 44-45 -0.4581661422504837 1106 46-47 -0.13497891832835052 1106 48-49 -0.06633629039159672 1106 50-51 -0.23906131100532235 1106 52-53 -0.3973815218397121 1106 54-55 -0.4623980634763569 1106 56-57 -0.5648776466395731 1106 58-59 -0.48413892291290495 1106 60-61 -0.7546171491680127 1106 62-63 -0.47832348027548477 1106 64-65 -0.06585392899084042 1106 66-67 0.028801610232534358 1106 68-69 -0.24066730900678834 1106 70-71 -0.21028117826756088 1106 72-73 -0.3437860635273502 1106 74-75 -0.47475778271064684 1106 76-77 -0.1303396946268336 1106 78-79 -0.13646653663315433 1106 80-81 0.023660914249564513 1106 82-83 -0.03173193530153995 1106 84-85 -0.4852409891755549 1106 86-87 -0.29621419322910114 1106 88-89 -0.23356708419864702 1106 90-91 -0.14222667011628332 1106 92-93 0.16091745069703833 1106 94-95 0.20886219053187105 1106 96-97 0.15120301525368873 1106 98-99 0.4271471640378657 1106 100-101 0.4896088297295407 1107 1 -0.2213177732994822 1107 2 -0.285977165773339 1107 3 -0.2106175162872077 1107 4 -0.18369702282009825 1107 5 -0.1455344208641307 1107 6 -0.18571423135426102 1107 7 -0.2575562284142734 1107 8 -0.08771327796100081 1107 9 -0.24865028799007405 1107 10-11 -0.16988976497668773 1107 12-13 -0.06519059610915434 1107 14-15 -0.22610486823499798 1107 16-17 -0.10585407168637317 1107 18-19 -0.3208279840806938 1107 20-21 -0.2526475182563388 1107 22-23 -0.19596351230061515 1107 24-25 -0.23137520124065958 1107 26-27 -0.2963867312466846 1107 28-29 -0.09274133626507108 1107 30-31 -0.1663780163977222 1107 32-33 -0.277041659387379 1107 34-35 -0.23988498845325523 1107 36-37 -0.15491481528321316 1107 38-39 -0.11153178164914124 1107 40-41 -0.2011169357642686 1107 42-43 -0.217164112531556 1107 44-45 -0.251607454691289 1107 46-47 -0.3138517252225128 1107 48-49 -0.3147292630756482 1107 50-51 -0.39187659780870376 1107 52-53 -0.4302115309356509 1107 54-55 -0.5066162816100928 1107 56-57 -0.39743055780882486 1107 58-59 -0.6696709884411689 1107 60-61 -0.5350369689371419 1107 62-63 -0.41271949119060736 1107 64-65 -0.2858900783142744 1107 66-67 -0.09187102036921146 1107 68-69 -0.24821224950809295 1107 70-71 -0.39070615552771315 1107 72-73 -0.4722033701917674 1107 74-75 -0.22810007132384413 1107 76-77 -0.438646537210829 1107 78-79 -0.12628986912253382 1107 80-81 -0.06395219890578474 1107 82-83 -0.1074818813823164 1107 84-85 -0.2995309902070531 1107 86-87 -0.10688996521173522 1107 88-89 0.01721615114235675 1107 90-91 0.09303861246733902 1107 92-93 -0.10736444838492432 1107 94-95 -0.13971316978920711 1107 96-97 0.09377247728048843 1107 98-99 0.014766841272699338 1107 100-101 0.20489539868519913 1108 1 4.1167535833608326E-4 1108 2 0.039636048384323885 1108 3 -0.122558015813091 1108 4 -0.04770107421051506 1108 5 -0.08575957697734538 1108 6 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-1.9960924213626932 2316 88-89 -1.683843429156049 2316 90-91 -2.065418636603294 2316 92-93 -1.7398762103055212 2316 94-95 -1.4729847971151457 2316 96-97 -1.583671332473596 2316 98-99 -1.970078667033686 2316 100-101 -2.6191883300703047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5972.0 3 1364.0 4 1291.0 5 1377.0 6 1600.0 7 1780.0 8 2242.0 9 2608.0 10 3383.0 11 4148.0 12 5311.0 13 6465.0 14 6961.0 15 8848.0 16 10601.0 17 13447.0 18 11771.0 19 8745.0 20 8987.0 21 9545.0 22 10586.0 23 11680.0 24 12951.0 25 14726.0 26 17226.0 27 19897.0 28 23837.0 29 28808.0 30 34378.0 31 42605.0 32 53584.0 33 68369.0 34 91819.0 35 126962.0 36 183063.0 37 250909.0 38 197733.0 39 24550.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11306801889497 19.30351186972729 14.838894674091849 22.744525437285883 2 14.822856841176405 20.152348773102148 40.778110045591504 24.24668434012995 3 19.171073549951885 24.74003662263303 29.595476772984924 26.49341305443016 4 12.25362533628685 16.262594494059403 37.77899458245373 33.70478558720002 5 13.399684885437226 37.7166789730795 34.17823959718434 14.705396544298937 6 31.66099482752384 36.28050434656796 17.64964311120221 14.408857714705988 7 28.501205545509343 32.659885473176615 20.560955394816155 18.27795358649789 8 26.40400079569841 34.820621247558655 19.651339425650423 19.12403853109252 9 26.2264807732299 16.830694719457025 20.41135854708969 36.531465960223386 10-11 24.695950936150073 26.969894281812675 27.313244767597844 21.02091001443941 12-13 25.703121585553866 24.139013263686795 28.069855517594238 22.088009633165097 14-15 22.605016974285963 25.383367196509525 26.51262531981017 25.498990509394346 16-17 21.772363261529375 29.02917720317195 26.876301679109538 22.322157856189133 18-19 21.665182972795094 27.508513188048124 29.462317629033336 21.36398621012345 20-21 22.37371865285833 26.425924837679705 29.57781655282774 21.622539956634228 22-23 22.076937739634637 26.386650603753086 29.45325820229477 22.08315345431751 24-25 21.85555764401351 26.60728498498759 29.585585069777302 21.951572301221596 26-27 21.76785932219478 26.77598798254033 29.504400324845076 21.951752370419815 28-29 21.624753746129354 26.952088198915487 29.604791971285543 21.818366083669616 30-31 21.727859264498147 26.580789414090656 29.608209369982703 22.083141951428498 32-33 21.50431735070873 26.74146457617323 29.5554473821234 22.19877069099464 34-35 21.971438260672972 26.757639700064058 29.389577602773276 21.881344436489695 36-37 21.91268932124048 26.46495002601066 29.464933828207606 22.157426824541254 38-39 21.732243779917642 26.866137710431115 29.484651881807512 21.916966627843728 40-41 21.838443154145896 26.84967728934349 29.473624736567533 21.838254819943085 42-43 21.617495214951425 26.805802556521208 29.201698320251126 22.37500390827624 44-45 21.8049800581435 26.87774984582592 29.03434098069405 22.282929115336525 46-47 22.024463672760795 27.11148651830631 28.643225735720453 22.220824073212437 48-49 21.96915692066554 27.268356643356643 28.407056305763202 22.35543013021461 50-51 21.934497870892553 27.332306176992677 28.51760540692686 22.215590545187908 52-53 22.17667425827316 27.468851863423442 28.126317899998078 22.228155978305317 54-55 21.903082910368667 27.24383525307988 28.376166080714132 22.476915755837325 56-57 21.982498371910918 27.573181144826293 28.23594240651341 22.20837807674938 58-59 22.121698229400856 27.213832737534073 28.356027189689403 22.308441843375668 60-61 21.99522127946777 27.258404943836474 28.69623939401244 22.050134382683314 62-63 21.90532942896769 27.333937018373472 28.707973388639317 22.052760164019517 64-65 21.82280353725519 27.408002778098968 28.496003657930746 22.2731900267151 66-67 21.718320458019925 27.50695741485853 28.23021218561694 22.544509941504607 68-69 21.7125037896834 27.370082120791565 28.497552947290917 22.419861142234115 70-71 21.82507397564077 27.321192891106648 28.38009166350274 22.47364146974984 72-73 21.873890026196793 27.234759812497998 28.34444170318712 22.546908458118086 74-75 21.72560269793515 27.71941534693576 28.173541145356793 22.381440809772293 76-77 21.866545521117974 27.373883942865714 28.17334662318084 22.586223912835475 78-79 21.928665089348506 27.401188149245286 28.138856232133218 22.531290529272987 80-81 21.876132422950036 27.574955569467956 28.0696996202216 22.47921238736041 82-83 21.934696552529388 27.465301187575093 28.196867031514245 22.403135228381274 84-85 21.871868196182685 27.45860134324137 28.0631842420531 22.60634621852284 86-87 21.79948936606141 27.46391797617519 28.17506932802939 22.561523329734012 88-89 21.902813773164844 27.295938640634233 28.056753797578725 22.744493788622197 90-91 21.772733967111773 27.232547268775022 28.3320357184963 22.662683045616905 92-93 21.908341082748688 27.14773681290557 28.287983717000014 22.655938387345724 94-95 21.717423563352593 26.988972332909 28.320540536468886 22.973063567269524 96-97 21.61823294437209 27.036510030108023 28.399544123662107 22.945712901857778 98-99 21.589382271014255 27.090953949536782 28.157397624307624 23.162266155141335 100-101 21.593845340375378 26.807439792805983 28.633380396634795 22.965334470183848 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1872.0 1 1024.5 2 199.5 3 238.0 4 222.5 5 191.0 6 310.0 7 693.5 8 1203.0 9 1499.5 10 1435.5 11 1338.0 12 1408.0 13 1507.5 14 1586.5 15 1677.5 16 1766.5 17 1826.5 18 1975.5 19 2151.5 20 2348.5 21 2734.0 22 3180.5 23 3811.0 24 4550.0 25 5467.0 26 7074.0 27 8943.0 28 10497.5 29 12580.5 30 15539.5 31 18110.0 32 21028.0 33 24159.0 34 27384.5 35 30305.0 36 33775.0 37 37496.0 38 39892.0 39 41611.5 40 42397.0 41 43545.5 42 44763.5 43 46761.5 44 49115.5 45 50336.5 46 50910.5 47 52531.5 48 56149.0 49 55403.5 50 50825.0 51 47445.5 52 44559.0 53 42853.0 54 41287.5 55 38126.0 56 33982.0 57 30003.0 58 26956.5 59 24068.5 60 20406.0 61 16477.5 62 12728.0 63 9354.0 64 6996.5 65 5416.0 66 3907.5 67 2766.0 68 1916.0 69 1310.5 70 937.0 71 639.0 72 419.0 73 312.5 74 243.5 75 175.5 76 126.0 77 81.5 78 53.0 79 34.5 80 25.5 81 15.5 82 13.5 83 11.0 84 6.5 85 6.0 86 4.5 87 6.0 88 5.5 89 4.0 90 4.0 91 2.5 92 4.5 93 5.5 94 3.0 95 3.0 96 4.5 97 4.0 98 2.5 99 4.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3695862678703531 2 0.31808777448321074 3 0.31425358008504395 4 0.3201928223880866 5 0.3209446252112566 6 0.23396103857049205 7 0.22072930888270073 8 0.22554084695098844 9 0.21982714549489676 10-11 0.24147906680219164 12-13 0.22960058219610624 14-15 0.22415001172812402 16-17 0.22584156808025646 18-19 0.22031581732995723 20-21 0.21215875669856316 22-23 0.21463970601502402 24-25 0.205580481995826 26-27 0.2055428918546675 28-29 0.22550325680983 30-31 0.23790800339213436 32-33 0.2292246807845213 34-35 0.23952437946194974 36-37 0.21016647921716275 38-39 0.20614433411320346 40-41 0.20362579465558409 42-43 0.21234670740435563 44-45 0.21821076942508136 46-47 0.22437555257507502 48-49 0.23275815405342012 50-51 0.23843426536835333 52-53 0.2594847444171122 54-55 0.2326453836299446 56-57 0.19994196082205126 58-59 0.19903979743424732 60-61 0.1942658495071181 62-63 0.2054677115723505 64-65 0.19618294670620146 66-67 0.19513042275376352 68-69 0.1893415410153548 70-71 0.20088171435101373 72-73 0.2017462875976592 74-75 0.20678336651289794 76-77 0.21103105246380824 78-79 0.20881323413545683 80-81 0.2092267256882003 82-83 0.19855112559918683 84-85 0.1899429832738908 86-87 0.19325091569583863 88-89 0.19640848755315246 90-91 0.20302435239704814 92-93 0.19670920868242048 94-95 0.19291260442541214 96-97 0.19419066922480108 98-99 0.205505301713509 100-101 0.18731167339279592 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1330136.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.24819392532378 #Duplication Level Percentage of deduplicated Percentage of total 1 80.86930367213905 49.530987939170714 2 11.090715841943853 13.585726293160755 3 3.334718945251833 6.127365379357064 4 1.4947944815637257 3.662138491412756 5 0.7850613155596307 2.4041793849333035 6 0.5116291508694727 1.8801816870181323 7 0.32846193739283447 1.408239030696672 8 0.23576962453376726 1.1552370948115964 9 0.18392128026594542 1.0138361616650207 >10 1.0663273640569348 11.95853331770585 >50 0.06863851370113584 2.825096570880156 >100 0.029186316667999215 2.9093561114695885 >500 7.357780269344031E-4 0.3218397459556463 >1k 6.131483557786693E-4 0.8215363223483919 >5k 1.2262967115573388E-4 0.3957464694143058 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5269 0.39612490752825275 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3657 0.27493429243325496 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2598 0.195318373459556 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2037 0.1531422350797212 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1579 0.11870966577853692 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.518028231699615E-5 0.0 20-21 0.0 0.0 0.0 1.1277042347549422E-4 3.7590141158498076E-5 22-23 0.0 0.0 0.0 2.631309881094865E-4 1.1277042347549422E-4 24-25 0.0 0.0 0.0 6.014422585359692E-4 1.503605646339923E-4 26-27 0.0 0.0 0.0 7.518028231699615E-4 1.503605646339923E-4 28-29 0.0 0.0 0.0 0.0012404746582304366 1.503605646339923E-4 30-31 0.0 0.0 0.0 0.002894440869204352 1.503605646339923E-4 32-33 0.0 0.0 0.0 0.005262619762189731 1.503605646339923E-4 34-35 7.518028231699615E-5 0.0 0.0 0.008232240913711079 2.2554084695098847E-4 36-37 7.518028231699615E-5 0.0 0.0 0.011239452206390926 2.2554084695098847E-4 38-39 7.518028231699615E-5 0.0 0.0 0.013382090252425316 2.631309881094865E-4 40-41 7.518028231699615E-5 0.0 0.0 0.017065924085958126 3.007211292679846E-4 42-43 7.518028231699615E-5 0.0 0.0 0.02247890441278185 3.007211292679846E-4 44-45 7.518028231699615E-5 0.0 0.0 0.027628753751496087 3.007211292679846E-4 46-47 7.518028231699615E-5 0.0 0.0 0.034282208736550246 3.007211292679846E-4 48-49 7.518028231699615E-5 0.0 0.0 0.040334221463068434 3.007211292679846E-4 50-51 7.518028231699615E-5 0.0 0.0 0.052889328610006794 3.007211292679846E-4 52-53 7.518028231699615E-5 0.0 0.0 0.08025495137339339 3.383112704264827E-4 54-55 7.518028231699615E-5 0.0 0.0 0.11840894464926895 3.7590141158498077E-4 56-57 1.503605646339923E-4 0.0 0.0 0.14596251811844804 3.7590141158498077E-4 58-59 1.503605646339923E-4 0.0 0.0 0.19246152273151018 3.7590141158498077E-4 60-61 1.503605646339923E-4 0.0 0.0 0.24670409642322289 3.7590141158498077E-4 62-63 1.503605646339923E-4 0.0 0.0 0.2770017501969724 3.7590141158498077E-4 64-65 1.503605646339923E-4 0.0 0.0 0.3051191757835289 3.7590141158498077E-4 66-67 1.503605646339923E-4 0.0 0.0 0.3998839216441026 3.7590141158498077E-4 68-69 1.503605646339923E-4 0.0 0.0 0.4875817209668786 3.7590141158498077E-4 70-71 2.631309881094865E-4 0.0 0.0 0.5321636283808573 3.7590141158498077E-4 72-73 3.7590141158498077E-4 0.0 0.0 0.6314391911804507 5.26261976218973E-4 74-75 3.7590141158498077E-4 0.0 0.0 0.7692822388086632 5.26261976218973E-4 76-77 3.7590141158498077E-4 0.0 0.0 0.8966376370536546 5.26261976218973E-4 78-79 3.7590141158498077E-4 0.0 0.0 1.0405326974083853 5.26261976218973E-4 80-81 4.5108169390197693E-4 0.0 0.0 1.2371667258084886 5.26261976218973E-4 82-83 4.5108169390197693E-4 0.0 0.0 1.4443635838741302 5.26261976218973E-4 84-85 4.88671835060475E-4 0.0 0.0 1.6281041938568688 5.26261976218973E-4 86-87 5.26261976218973E-4 0.0 0.0 1.9141651680730392 5.26261976218973E-4 88-89 6.014422585359692E-4 0.0 0.0 2.2665727414339587 6.014422585359692E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7905 0.0 29.477104 1 GTACATG 5950 0.0 25.495546 1 TATCAAC 9095 0.0 25.243801 2 ATCAACG 9120 0.0 25.121532 3 TCAACGC 9175 0.0 24.976595 4 CAACGCA 9225 0.0 24.738146 5 TACATGG 5845 0.0 24.641575 2 AACGCAG 9490 0.0 24.179401 6 GGTATCA 5475 0.0 23.625196 1 GTACACG 185 2.5143345E-7 23.134567 1 GTAGCAC 395 0.0 22.863909 3 ACATGGG 6155 0.0 22.472889 3 GAGTACT 4515 0.0 22.463642 12-13 CGCAGAG 10405 0.0 21.870466 8 ACGCAGA 10480 0.0 21.804615 7 TAAGGTG 440 0.0 21.610739 5 GTACCTG 1105 0.0 21.517822 1 TACCTGG 1105 0.0 21.508894 2 GTACTTT 4785 0.0 21.194504 14-15 GCAGAGT 10860 0.0 20.079248 9 >>END_MODULE