FastQCFastQC Report
Thu 26 May 2016
SRR937334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937334_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330136
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60790.45702093620501966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47010.3534225071721989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33080.24869637390462326No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21730.16336675347483265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87300.027.816011
GGTATCA62300.027.2312981
GTACATG66500.024.510981
ATCAACG104150.023.1701763
TCAACGC104050.023.1467884
TATCAAC104800.023.1171232
TACATGG67250.023.0983182
CAACGCA105350.022.770985
ACATGGG66650.022.450983
AACGCAG108600.022.2182466
GAGTACT53800.021.98578512-13
AGTACTT55400.021.47943512-13
GTACTTT55800.021.36561814-15
TATCACG1404.8499336E-420.358612
ACGCAGA122700.019.3962617
CGCAGAG122250.019.3510868
AGAGTAC95150.019.07126410-11
CATGGGG29050.018.9686644
TACCTGG10650.018.7337462
CCGTGCG1300.00756059618.2705469
Produced by FastQC (version 0.11.5)