FastQCFastQC Report
Thu 26 May 2016
SRR937333_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937333_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449442
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57390.39594547418937764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.27355354681318744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.2009048999546032No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22870.157784857897039No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17070.11776945886761939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81900.029.4411
GGTATCA56100.026.5344431
GTACATG66650.025.25991
TCAACGC96450.024.7428424
ATCAACG96450.024.7419853
CAACGCA97150.024.4169165
TACATGG66750.024.215352
TATCAAC98650.024.1918872
AACGCAG99050.024.1734496
ACATGGG67300.023.0269053
GAGTACT49200.022.97811112-13
ACGCAGA111650.021.3610487
AGTACTT52150.021.35949512-13
CGCAGAG110900.021.1620628
GTACTTT53100.021.15590514-15
GTACCTG11600.020.9093571
GCAGAGT115400.019.9224159
AGAGTAC89400.019.658910-11
TACCTGG11900.019.5754722
TAAGCGT1507.680096E-419.0155144
Produced by FastQC (version 0.11.5)