##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937333_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1449442 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.385302757888898 31.0 31.0 34.0 30.0 34.0 2 31.479712192692084 33.0 31.0 34.0 30.0 34.0 3 31.525849947773004 33.0 31.0 34.0 28.0 34.0 4 35.16651580401285 37.0 35.0 37.0 33.0 37.0 5 35.14547391340944 37.0 35.0 37.0 33.0 37.0 6 35.201630006581844 37.0 35.0 37.0 32.0 37.0 7 35.12478112266652 37.0 35.0 37.0 32.0 37.0 8 35.11323805988788 37.0 35.0 37.0 32.0 37.0 9 36.68535891743167 39.0 37.0 39.0 32.0 39.0 10-11 36.57793619889585 39.0 37.0 39.0 32.0 39.0 12-13 36.449383969831146 39.0 37.0 39.0 32.0 39.0 14-15 37.54424047323039 40.0 37.0 41.0 32.0 41.0 16-17 37.485441638920356 40.0 37.0 41.0 32.0 41.0 18-19 37.43812308460773 40.0 37.0 41.0 32.0 41.0 20-21 37.36212383800111 40.0 37.0 41.0 32.0 41.0 22-23 37.21304991852037 39.5 37.0 41.0 31.0 41.0 24-25 37.05010065942618 39.0 36.0 41.0 31.0 41.0 26-27 36.88081654871323 39.0 36.0 41.0 30.5 41.0 28-29 36.69513854297033 39.0 36.0 40.5 30.0 41.0 30-31 36.69790926439278 39.0 36.0 41.0 30.0 41.0 32-33 36.560920685339596 39.0 36.0 41.0 30.0 41.0 34-35 36.35520082900868 39.0 35.0 40.0 30.0 41.0 36-37 36.36588873511324 39.0 35.0 41.0 30.0 41.0 38-39 36.344272140589275 39.0 35.0 41.0 30.0 41.0 40-41 36.186763250961405 39.0 35.0 41.0 29.5 41.0 42-43 35.9516796808703 39.0 35.0 41.0 29.0 41.0 44-45 35.73668453101263 39.0 35.0 40.0 27.5 41.0 46-47 35.42020963929568 39.0 35.0 40.0 26.5 41.0 48-49 35.12042668833938 38.5 34.5 40.0 25.5 41.0 50-51 34.206643315151624 37.5 33.5 39.5 23.5 40.5 52-53 34.35252980112347 38.0 33.5 39.5 24.0 40.5 54-55 34.70580402665301 38.0 34.0 40.0 24.0 41.0 56-57 34.47437393148536 38.0 34.0 40.0 23.5 41.0 58-59 34.224218699333946 38.0 34.0 40.0 23.0 41.0 60-61 33.929708122160115 38.0 33.0 40.0 22.0 41.0 62-63 33.60014060583314 37.0 33.0 40.0 22.0 41.0 64-65 33.23345397746167 37.0 32.5 39.5 20.5 41.0 66-67 32.830119797825645 36.0 32.0 39.0 19.5 41.0 68-69 32.321391956352855 36.0 31.5 39.0 17.5 40.0 70-71 31.881812449204592 35.0 31.0 38.5 13.5 40.0 72-73 31.456323536919726 35.0 31.0 37.5 10.5 40.0 74-75 30.985718642070534 35.0 30.5 37.0 7.5 39.0 76-77 30.53837580255022 34.5 30.0 36.5 7.0 39.0 78-79 30.04192682425375 34.0 29.5 36.0 2.0 38.5 80-81 29.573115723154153 34.0 29.0 35.5 2.0 37.5 82-83 29.11160570757574 34.0 29.0 35.0 2.0 37.0 84-85 28.632194665257387 34.0 28.5 35.0 2.0 36.5 86-87 28.17912272446914 34.0 27.0 35.0 2.0 36.0 88-89 27.772055039111603 33.5 26.5 35.0 2.0 36.0 90-91 27.302199398113203 33.0 26.0 35.0 2.0 35.0 92-93 26.827201088418853 33.0 25.0 35.0 2.0 35.0 94-95 26.240859241004472 32.5 24.5 35.0 2.0 35.0 96-97 25.57257206566389 32.0 21.5 34.5 2.0 35.0 98-99 24.805507222779525 32.0 17.5 34.0 2.0 35.0 100-101 22.951504441019374 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1372245627305908 1101 2 0.14045299879218476 1101 3 0.14161829670182868 1101 4 0.04261891691621145 1101 5 0.22459397213114585 1101 6 -0.007459396943517049 1101 7 0.12187208952111916 1101 8 0.18218459962486122 1101 9 0.06863492223912715 1101 10-11 0.1508428031176905 1101 12-13 0.18241227421883366 1101 14-15 0.27660624565198333 1101 16-17 0.21356511467377715 1101 18-19 0.2324009462496761 1101 20-21 0.26531527492039686 1101 22-23 0.05363522883437355 1101 24-25 0.23788494213442135 1101 26-27 0.32278517948881813 1101 28-29 0.3895752345918666 1101 30-31 0.1800203999842367 1101 32-33 0.07781101270327184 1101 34-35 0.11628049414904496 1101 36-37 0.1753872393094298 1101 38-39 0.2139108973286028 1101 40-41 0.17764798886371125 1101 42-43 0.43373706130836354 1101 44-45 0.2647392960866526 1101 46-47 0.33498827485434646 1101 48-49 0.3107503046646869 1101 50-51 0.39009214989130214 1101 52-53 0.4268729102794637 1101 54-55 0.45189013426728764 1101 56-57 0.5492871283980918 1101 58-59 0.5089127983793205 1101 60-61 0.41806188960740087 1101 62-63 0.39878671588420644 1101 64-65 0.31809049215550544 1101 66-67 0.5182949350896635 1101 68-69 0.37712384871771576 1101 70-71 0.551043914200914 1101 72-73 0.6893129755418812 1101 74-75 0.4721282093988144 1101 76-77 0.572894443777745 1101 78-79 0.7934562468368433 1101 80-81 0.9521627824631054 1101 82-83 0.7390718658334876 1101 84-85 0.6970250347180844 1101 86-87 0.8223710202379024 1101 88-89 0.9697003413415537 1101 90-91 0.9662419158149653 1101 92-93 0.7632427065462615 1101 94-95 0.7747513104264421 1101 96-97 0.7411877488419876 1101 98-99 1.1012589910216342 1101 100-101 1.3512557230364841 1102 1 0.09206431245386071 1102 2 -0.06389765431202221 1102 3 0.06679773385565113 1102 4 -0.2330308751840633 1102 5 -0.021684890313949268 1102 6 -0.028643590604595204 1102 7 0.09248895264622803 1102 8 0.10099617652119264 1102 9 0.05576528134321279 1102 10-11 -0.010668587115894468 1102 12-13 0.13763170437864147 1102 14-15 0.1437879253438794 1102 16-17 0.21301327653576152 1102 18-19 0.14964174119818807 1102 20-21 0.18737756261800342 1102 22-23 0.35667874003288347 1102 24-25 0.2550193906571181 1102 26-27 0.35731473657858004 1102 28-29 0.27638432839609806 1102 30-31 0.30995458511821283 1102 32-33 0.10760937456618791 1102 34-35 0.18074628287725147 1102 36-37 0.1587297699450687 1102 38-39 0.3394328906096007 1102 40-41 0.12148059628634655 1102 42-43 0.1533223246091211 1102 44-45 0.1898399248661775 1102 46-47 0.3842641533522979 1102 48-49 0.24105551747062748 1102 50-51 0.2246028216940985 1102 52-53 0.24987109678926345 1102 54-55 0.4148728176080638 1102 56-57 0.3599696663652452 1102 58-59 0.2638616591046343 1102 60-61 0.2528915647840577 1102 62-63 0.31774819025784495 1102 64-65 0.3827401072510952 1102 66-67 0.4747778124893216 1102 68-69 0.32073726473939956 1102 70-71 0.1093289003618807 1102 72-73 0.11392172517431476 1102 74-75 -0.016347098088484557 1102 76-77 0.002015376754005871 1102 78-79 0.07580744352136293 1102 80-81 0.21793621574724042 1102 82-83 0.2882509841634544 1102 84-85 0.22298458501846952 1102 86-87 0.26519096697851907 1102 88-89 0.2901316008717103 1102 90-91 0.29012279202047253 1102 92-93 0.17849354516430438 1102 94-95 -0.027273436480193425 1102 96-97 0.2964597059501273 1102 98-99 0.5676740573158128 1102 100-101 0.8007526599219297 1103 1 0.09412436058950036 1103 2 -0.011616210814260342 1103 3 0.2543860241835283 1103 4 -0.03795656490203214 1103 5 0.12090836355510959 1103 6 0.1240560044561434 1103 7 0.0820273076072695 1103 8 0.12044290236946154 1103 9 0.24774469871632476 1103 10-11 0.13699194237612033 1103 12-13 0.20379106618865706 1103 14-15 0.28284653083876776 1103 16-17 0.3295393690504582 1103 18-19 0.3643866723156677 1103 20-21 0.25349585915923 1103 22-23 0.2517126992247185 1103 24-25 0.28101834795637615 1103 26-27 0.20640737268315945 1103 28-29 -0.01079420196720804 1103 30-31 0.04759554611691641 1103 32-33 -0.026741722769209275 1103 34-35 0.02520425703826845 1103 36-37 0.11106477683689064 1103 38-39 0.20431083748900392 1103 40-41 0.29297112270738523 1103 42-43 0.2538334979331154 1103 44-45 0.10406832632024532 1103 46-47 0.03561002290043547 1103 48-49 -0.08715257274440802 1103 50-51 0.02560713533276271 1103 52-53 0.08069790134001664 1103 54-55 0.13917202986907284 1103 56-57 0.11173011658343057 1103 58-59 -0.0294759473972519 1103 60-61 0.19529948930966157 1103 62-63 0.3909234639667858 1103 64-65 0.29844650129199124 1103 66-67 0.37538629264587087 1103 68-69 0.3802677994449155 1103 70-71 0.2001142111366896 1103 72-73 0.3312278792512018 1103 74-75 0.4059297389573864 1103 76-77 0.4643744157553016 1103 78-79 0.5153427632234084 1103 80-81 0.49649407492550424 1103 82-83 0.3795033677344932 1103 84-85 0.22914758913927713 1103 86-87 0.4340483169474538 1103 88-89 0.4181291869480859 1103 90-91 0.5182580360195885 1103 92-93 0.5639246590686469 1103 94-95 0.5937248423494133 1103 96-97 0.7955166331253345 1103 98-99 1.0264611321117343 1103 100-101 1.2918884538385562 1104 1 0.07312687032299792 1104 2 -0.01809662238111187 1104 3 0.11353698354939823 1104 4 -0.051009387064233636 1104 5 -0.03328605615181601 1104 6 -0.019297985725714284 1104 7 0.011507108300577329 1104 8 0.07459463452716619 1104 9 0.1916573069991827 1104 10-11 0.03514294590151934 1104 12-13 0.07647029869595912 1104 14-15 0.21589055789383593 1104 16-17 0.12670285500404788 1104 18-19 0.084681658000612 1104 20-21 0.11274507348752394 1104 22-23 0.17361619592379185 1104 24-25 0.1305123532996859 1104 26-27 0.17319719538263456 1104 28-29 -0.05486339259655182 1104 30-31 -0.0926291891098785 1104 32-33 -0.1780563888863398 1104 34-35 -0.05872719264561965 1104 36-37 -0.13986735731977262 1104 38-39 -0.08805710710549874 1104 40-41 -0.2907297774111157 1104 42-43 -0.010744116127277437 1104 44-45 0.08151071652860509 1104 46-47 0.06532497311056318 1104 48-49 0.07646420346242877 1104 50-51 -0.06510151478389048 1104 52-53 -0.0988783143351526 1104 54-55 0.11849359223337075 1104 56-57 -0.01775115609433442 1104 58-59 -0.001054319529636416 1104 60-61 0.16902898272621059 1104 62-63 0.2737437493845718 1104 64-65 0.11080252281240632 1104 66-67 0.22339212170431466 1104 68-69 0.2668567330657723 1104 70-71 0.3186137437816221 1104 72-73 0.30350544400011614 1104 74-75 0.29897626838603486 1104 76-77 0.34778791090879224 1104 78-79 0.19305967853278005 1104 80-81 0.23827156975515962 1104 82-83 0.31633867475364497 1104 84-85 0.3962697123296586 1104 86-87 0.17142549824441744 1104 88-89 0.2519248460659256 1104 90-91 0.3491676856136898 1104 92-93 0.35644513805238986 1104 94-95 0.5155749796119622 1104 96-97 0.5262211248859003 1104 98-99 0.39931792274402866 1104 100-101 0.7240036587019922 1105 1 0.040733074247441436 1105 2 0.09036395683277476 1105 3 0.06116322848130551 1105 4 0.12320622757916055 1105 5 -0.014924464894271239 1105 6 0.040900083509470164 1105 7 0.03851308521802821 1105 8 0.0505131515392776 1105 9 0.08541274838156454 1105 10-11 0.09655284671914188 1105 12-13 0.22250679479371627 1105 14-15 0.20880018632640684 1105 16-17 0.13061151249374348 1105 18-19 0.16364046661443155 1105 20-21 0.1694894324338705 1105 22-23 0.15769576580037636 1105 24-25 0.07067960277833407 1105 26-27 0.13755091566476807 1105 28-29 -0.01561175352288302 1105 30-31 0.013602732085495006 1105 32-33 -0.16445482853409032 1105 34-35 0.017435051636141452 1105 36-37 0.0645960246799504 1105 38-39 0.013641284675586007 1105 40-41 -0.003299190883069514 1105 42-43 0.01611157267639385 1105 44-45 0.11035106389051208 1105 46-47 -0.04216485765297051 1105 48-49 -0.16520258309773084 1105 50-51 -0.19753003460734675 1105 52-53 -0.08514765785412237 1105 54-55 0.011651380754479135 1105 56-57 0.0614570957924343 1105 58-59 0.15584860990260552 1105 60-61 0.15342837659655828 1105 62-63 0.054755303099256025 1105 64-65 -0.020885249645559156 1105 66-67 0.0426437374473565 1105 68-69 0.07151407033368429 1105 70-71 0.035127977213090134 1105 72-73 0.10127285865960545 1105 74-75 -0.030053212581421462 1105 76-77 -0.0014418090420456053 1105 78-79 0.12236710502089565 1105 80-81 0.09391993367558982 1105 82-83 0.19746330423414804 1105 84-85 -0.014437956097044236 1105 86-87 0.13846515680848626 1105 88-89 0.2461448779744444 1105 90-91 0.2543640769880433 1105 92-93 0.3534850649186545 1105 94-95 0.3069540801152435 1105 96-97 0.27592317335371774 1105 98-99 0.20764244552579925 1105 100-101 0.4615162329371252 1106 1 0.20311811639757593 1106 2 0.2591746658181151 1106 3 0.029031018232796413 1106 4 0.16017017752097473 1106 5 0.28206150113927464 1106 6 0.2174246074200994 1106 7 0.23227837955405306 1106 8 0.10565329740018825 1106 9 0.19472531400563042 1106 10-11 0.030273729877954736 1106 12-13 -0.05917256098580026 1106 14-15 -0.05541789357064175 1106 16-17 0.003823243384061925 1106 18-19 0.05613716549270009 1106 20-21 0.07724579802158615 1106 22-23 0.2543277602808445 1106 24-25 0.07422147168266946 1106 26-27 0.24797526808071524 1106 28-29 0.4621654308808303 1106 30-31 0.4668396402199875 1106 32-33 0.5567205430735953 1106 34-35 0.38317823685469676 1106 36-37 0.22429904000593837 1106 38-39 0.1580129259511054 1106 40-41 0.5446230619936188 1106 42-43 0.47491172151757866 1106 44-45 0.46873035739447744 1106 46-47 0.5845331851026998 1106 48-49 0.7310065318743355 1106 50-51 0.6972494556288282 1106 52-53 0.5938808088835827 1106 54-55 0.5336737172681936 1106 56-57 0.5516644139895561 1106 58-59 0.4626776466813638 1106 60-61 0.5689719871494674 1106 62-63 0.8168568320806457 1106 64-65 0.7166667435601255 1106 66-67 0.8064849769636524 1106 68-69 0.9445287675392109 1106 70-71 0.8941496220632139 1106 72-73 1.0659764812369517 1106 74-75 0.8752162218306943 1106 76-77 0.9341019048727972 1106 78-79 0.8907446413719988 1106 80-81 0.8373258730245325 1106 82-83 0.7146681133203501 1106 84-85 0.9787905452669037 1106 86-87 0.7466691657010607 1106 88-89 1.048801538057532 1106 90-91 1.2510119202982466 1106 92-93 1.3204639420462954 1106 94-95 1.548069691298661 1106 96-97 1.8220275482336703 1106 98-99 1.728003023629089 1106 100-101 1.7348140126366687 1107 1 -0.02707331671766866 1107 2 -0.12837189333816923 1107 3 0.019106245274077338 1107 4 -0.13244016942321224 1107 5 -0.08255859707089996 1107 6 0.099195738650657 1107 7 -0.05946443254923395 1107 8 -0.026962699419136982 1107 9 0.07988201085146329 1107 10-11 0.03062161570861832 1107 12-13 0.08423138416158338 1107 14-15 0.17386938514257366 1107 16-17 0.059447920783163966 1107 18-19 0.07804568982901827 1107 20-21 0.2990282464403151 1107 22-23 0.17902530014385576 1107 24-25 0.10894422672920001 1107 26-27 0.06573540646778042 1107 28-29 0.1081615178191555 1107 30-31 0.13657828118088133 1107 32-33 0.1262214184554793 1107 34-35 0.1662496240734228 1107 36-37 0.3310110568021116 1107 38-39 0.2624270652867935 1107 40-41 0.34240287031637706 1107 42-43 0.47789883143206424 1107 44-45 0.36161552542594677 1107 46-47 0.4082143577656723 1107 48-49 0.349865344284531 1107 50-51 0.40362073612769933 1107 52-53 0.4562902190645204 1107 54-55 0.4401198934885002 1107 56-57 0.5001834143104134 1107 58-59 0.44478811917833383 1107 60-61 0.3111942539351773 1107 62-63 0.18878863599510964 1107 64-65 0.35075754960897143 1107 66-67 0.3208154318832328 1107 68-69 0.31693793358973466 1107 70-71 0.4856292092735721 1107 72-73 0.5534456161782941 1107 74-75 0.49808444023673104 1107 76-77 0.49916745388720685 1107 78-79 0.5655904032681214 1107 80-81 0.5269118801670913 1107 82-83 0.5378610251683469 1107 84-85 0.6499516817985764 1107 86-87 0.589943547353613 1107 88-89 0.7298094130069614 1107 90-91 0.5981269963971805 1107 92-93 0.5077965383383045 1107 94-95 0.34017869901317965 1107 96-97 0.4090064170031127 1107 98-99 0.684254418659993 1107 100-101 1.1405897021306934 1108 1 -0.06779362384825305 1108 2 -0.08516823139659024 1108 3 -0.06464321778434368 1108 4 -0.08600814622733566 1108 5 0.046757070884424934 1108 6 0.03454797232762985 1108 7 0.04529286985626868 1108 8 0.07790870307226783 1108 9 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-1.5099217562918525 2316 98-99 -1.7535808472660328 2316 100-101 -2.005412951412765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6701.0 3 1492.0 4 1368.0 5 1485.0 6 1774.0 7 2023.0 8 2558.0 9 3011.0 10 3928.0 11 4697.0 12 5779.0 13 7060.0 14 8184.0 15 9708.0 16 11560.0 17 14680.0 18 13031.0 19 9872.0 20 10178.0 21 10916.0 22 11658.0 23 13261.0 24 14710.0 25 16842.0 26 19536.0 27 22692.0 28 27076.0 29 32399.0 30 39053.0 31 48089.0 32 59345.0 33 75981.0 34 100754.0 35 138219.0 36 197239.0 37 266368.0 38 209329.0 39 26877.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.071489109481284 19.318830183244334 14.835769759145597 22.77391094812878 2 14.8748981680986 20.193759201208763 40.66589930806218 24.265443322630457 3 19.20910250562539 24.74451286658467 29.572276643855954 26.474107983933987 4 12.300824009440982 16.268597315134294 37.71782954321291 33.71274913221181 5 13.473541430939207 37.74089183699598 34.126692479904776 14.658874252160043 6 31.638277436526163 36.31235275528698 17.642812245228182 14.406557562958675 7 28.529787035404198 32.69592639080508 20.604350577276172 18.169935996514557 8 26.482535704505285 34.77624346835072 19.673230818596494 19.067990008547504 9 26.238470698389737 16.880806141769092 20.36480238545382 36.515920774387354 10-11 24.652437390319598 27.034864320478576 27.278093311548535 21.03460497765329 12-13 25.68898005363844 24.23643374933782 27.991677605053468 22.08290859197028 14-15 22.620477408390013 25.388327328218097 26.45562679643524 25.535568466956654 16-17 21.82045888017084 29.06461223118001 26.878645295226555 22.2362835934226 18-19 21.694601172696096 27.502765792676183 29.41793893129771 21.384694103330016 20-21 22.43757094113014 26.437380093715763 29.506842675003103 21.618206290150994 22-23 22.155269397438406 26.44202878007259 29.41362555481262 21.989076267676385 24-25 21.987765216044604 26.673398471205118 29.44095140379212 21.89788490895816 26-27 21.78678388995726 26.796260914482733 29.45083045841692 21.96612473714309 28-29 21.629122769408436 26.95875415345571 29.502795478827316 21.909327598308536 30-31 21.77358032554921 26.683513646503197 29.485638053426914 22.057267974520673 32-33 21.54745557399447 26.832238813402302 29.441969465020605 22.178336147582623 34-35 22.069631739465812 26.728009423730054 29.295851394387736 21.906507442416398 36-37 21.942124316451054 26.531742566226935 29.403434676800664 22.122698440521347 38-39 21.7227929705257 26.890120993877538 29.42950251158829 21.957583524008477 40-41 21.904605191517987 26.866065832698705 29.325726288105088 21.903602687678227 42-43 21.70399265464381 26.804120147460235 29.14075096311005 22.351136234785905 44-45 21.855956052208747 26.90619706592895 29.008329071811055 22.229517810051245 46-47 22.00837207758623 27.19680605378375 28.59038912044784 22.204432748182178 48-49 22.02047108458438 27.279727347254333 28.34667449467996 22.35312707348133 50-51 21.934803431689527 27.361776290082197 28.50096996061372 22.202450317614552 52-53 22.177513065756237 27.475611510592323 28.10344565226413 22.24342977138731 54-55 21.973775714127136 27.310687968207453 28.275952218503207 22.439584099162204 56-57 22.07338403041825 27.5621500172831 28.125337020394053 22.2391289319046 58-59 22.21347813269104 27.208323267261886 28.286161908050193 22.292036691996877 60-61 22.09529530871998 27.250093412201693 28.63065835122366 22.023952927854673 62-63 21.97125739313121 27.354248464513486 28.645823431754025 22.02867071060128 64-65 21.874522577875542 27.355484106898313 28.451178683936014 22.318814631290135 66-67 21.788932549719938 27.498683779776567 28.17931568993274 22.533067980570756 68-69 21.753261566879402 27.439331261268237 28.379503146810602 22.427904025041755 70-71 21.8936874779639 27.33838464144239 28.33355225407711 22.434375626516605 72-73 21.940948984297915 27.159062701168065 28.3311011097044 22.568887204829625 74-75 21.76328023968366 27.73680842833602 28.063238100769045 22.436673231211284 76-77 21.94171877344459 27.403440257386624 28.094236804821364 22.560604164347417 78-79 22.009428942920568 27.347391587380553 28.11940054961258 22.523778920086304 80-81 21.930531959204767 27.579748944429372 27.975222823485087 22.51449627288078 82-83 21.983374789764902 27.4821397395764 28.14548873934996 22.388996731308737 84-85 21.915096589554018 27.49921366528756 27.954665643567438 22.631024101590988 86-87 21.803966789827335 27.469184771769072 28.14693140507209 22.5799170333315 88-89 21.939768345107368 27.33195859181758 27.994530994774713 22.733742068300337 90-91 21.77937544765945 27.236603402073545 28.286822841624655 22.69719830864235 92-93 21.948248104047245 27.20920801947308 28.183380470710006 22.659163405769668 94-95 21.797693224590486 27.06357667725886 28.22083620262897 22.917893895521686 96-97 21.654141160227965 27.086861371144565 28.33184174894578 22.92715571968169 98-99 21.643901916249224 27.084763692556603 28.129038280042728 23.142296111151445 100-101 21.661474099261795 26.790571670909895 28.54569862555379 23.00225560427452 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2059.0 1 1182.0 2 283.0 3 260.0 4 224.5 5 215.5 6 370.0 7 785.0 8 1364.5 9 1647.0 10 1559.0 11 1460.0 12 1472.0 13 1603.0 14 1742.5 15 1854.5 16 1986.0 17 2063.5 18 2168.5 19 2310.0 20 2512.5 21 2902.0 22 3419.5 23 4045.5 24 4779.0 25 5910.0 26 7617.5 27 9629.5 28 11422.5 29 13645.0 30 16557.0 31 19382.5 32 22700.0 33 26075.0 34 29781.5 35 33108.0 36 36747.0 37 40785.5 38 43328.0 39 45189.0 40 46232.5 41 47440.5 42 49002.0 43 51090.5 44 53314.5 45 54815.5 46 55235.0 47 56781.5 48 61103.0 49 60734.5 50 55824.5 51 51750.5 52 48557.0 53 46729.5 54 44832.0 55 41561.5 56 37237.0 57 32944.0 58 29547.0 59 26363.0 60 22464.0 61 18123.0 62 14005.5 63 10351.0 64 7774.5 65 6018.5 66 4307.0 67 3025.5 68 2137.0 69 1482.0 70 1070.5 71 736.5 72 491.5 73 361.5 74 288.5 75 205.0 76 127.5 77 71.0 78 45.5 79 32.0 80 18.5 81 16.5 82 14.0 83 11.0 84 8.5 85 7.0 86 5.5 87 4.0 88 5.0 89 5.0 90 3.0 91 2.0 92 2.5 93 3.5 94 2.5 95 1.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36220835328353945 2 0.3214340415139067 3 0.3210200891101541 4 0.32267589872516456 5 0.30680772324798095 6 0.22732886172747857 7 0.23643581461003615 8 0.23498698119690198 9 0.23070947302479158 10-11 0.2400578981428715 12-13 0.23898852109984392 14-15 0.21904981365242623 16-17 0.23457302879314937 18-19 0.22091259946931302 20-21 0.22494863540590102 22-23 0.2230513535553682 24-25 0.21208161485592383 26-27 0.22857071893873643 28-29 0.23160636989958897 30-31 0.24047185054662415 32-33 0.24330052530560034 34-35 0.22805327843404563 36-37 0.22225794478150904 38-39 0.21018433300539102 40-41 0.21135719814935677 42-43 0.2129095196634291 44-45 0.2319858262696955 46-47 0.22118856773848142 48-49 0.23136489766406657 50-51 0.24195517999340438 52-53 0.25375282350035394 54-55 0.2461981921318687 56-57 0.2029746619733663 58-59 0.18976268108692865 60-61 0.1998355229115756 62-63 0.2010083880555414 64-65 0.198076225195627 66-67 0.21149518228394099 68-69 0.1999390110125138 70-71 0.2036645826462873 72-73 0.22084360740202091 74-75 0.20942542026517794 76-77 0.22167151220952616 78-79 0.21735950800376974 80-81 0.20349210247805707 82-83 0.2169455556000171 84-85 0.19711033625353755 86-87 0.2040440390163939 88-89 0.21453083324479352 90-91 0.20904596389507132 92-93 0.2164626111289724 94-95 0.21346145620176593 96-97 0.20652775343890958 98-99 0.2157381944224053 100-101 0.19476460596560607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1449442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.03931318101695 #Duplication Level Percentage of deduplicated Percentage of total 1 80.23845166181543 48.17461528483627 2 11.29643061159522 13.564598706343844 3 3.4943857897653987 6.294015684210601 4 1.5648544279723793 3.758111403349393 5 0.8448229985625593 2.5361296296611666 6 0.5358640637986212 1.9303746209314718 7 0.3582194560906954 1.5055075078233968 8 0.2557417887196625 1.228364907713063 9 0.18832829682320745 1.0176391434434464 >10 1.1149141798281696 12.257015869247336 >50 0.07610164201995004 3.100462844985012 >100 0.030503816227594755 3.09114441281375 >500 5.755278254718422E-4 0.2439533252267288 >1k 6.906333905662106E-4 0.9014524572967281 >5k 1.1510556509436842E-4 0.3966142021179027 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5739 0.39594547418937764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3965 0.27355354681318744 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2912 0.2009048999546032 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2287 0.157784857897039 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1707 0.11776945886761939 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.899206729210275E-5 7 0.0 0.0 0.0 0.0 6.899206729210275E-5 8 0.0 0.0 0.0 0.0 6.899206729210275E-5 9 0.0 0.0 0.0 0.0 6.899206729210275E-5 10-11 0.0 0.0 0.0 0.0 6.899206729210275E-5 12-13 0.0 0.0 0.0 0.0 6.899206729210275E-5 14-15 0.0 0.0 0.0 0.0 6.899206729210275E-5 16-17 0.0 0.0 0.0 0.0 6.899206729210275E-5 18-19 0.0 0.0 0.0 0.0 1.379841345842055E-4 20-21 0.0 0.0 0.0 0.0 1.379841345842055E-4 22-23 0.0 0.0 0.0 1.379841345842055E-4 1.379841345842055E-4 24-25 0.0 0.0 0.0 2.0697620187630825E-4 1.7248016823025688E-4 26-27 0.0 0.0 0.0 3.104643028144624E-4 2.0697620187630825E-4 28-29 0.0 0.0 0.0 9.313929084433872E-4 2.0697620187630825E-4 30-31 0.0 0.0 0.0 0.0029666588935604184 2.0697620187630825E-4 32-33 0.0 0.0 0.0 0.005933317787120837 2.0697620187630825E-4 34-35 1.0348810093815413E-4 0.0 0.0 0.008624008411512844 2.0697620187630825E-4 36-37 1.379841345842055E-4 0.0 0.0 0.011142218867674594 2.0697620187630825E-4 38-39 2.0697620187630825E-4 0.0 0.0 0.014108877761235013 2.0697620187630825E-4 40-41 2.0697620187630825E-4 0.0 0.0 0.0188693304043901 2.0697620187630825E-4 42-43 2.0697620187630825E-4 0.0 0.0 0.024147223552235964 2.0697620187630825E-4 44-45 2.0697620187630825E-4 0.0 0.0 0.029908061171126543 2.0697620187630825E-4 46-47 2.0697620187630825E-4 0.0 0.0 0.03704874013585918 2.75968269168411E-4 48-49 2.0697620187630825E-4 0.0 0.0 0.044534379437052324 3.4496033646051375E-4 50-51 2.0697620187630825E-4 0.0 0.0 0.05615954277577164 3.4496033646051375E-4 52-53 2.0697620187630825E-4 0.0 0.0 0.08882728663858229 4.139524037526165E-4 54-55 2.0697620187630825E-4 0.0 0.0 0.13218880093166888 4.8294447104471925E-4 56-57 2.75968269168411E-4 0.0 0.0 0.16237283037196382 4.8294447104471925E-4 58-59 2.75968269168411E-4 0.0 0.0 0.2110122378128963 4.8294447104471925E-4 60-61 2.75968269168411E-4 0.0 0.0 0.26975898311212176 4.8294447104471925E-4 62-63 2.75968269168411E-4 0.0 0.0 0.30432400882546523 4.8294447104471925E-4 64-65 2.75968269168411E-4 0.0 0.0 0.33499098273680494 4.8294447104471925E-4 66-67 3.4496033646051375E-4 0.0 0.0 0.42750934497551474 4.8294447104471925E-4 68-69 3.4496033646051375E-4 0.0 0.0 0.5135769489224129 4.8294447104471925E-4 70-71 3.4496033646051375E-4 0.0 0.0 0.5563175346098705 4.8294447104471925E-4 72-73 3.4496033646051375E-4 0.0 0.0 0.6550451829048696 5.51936538336822E-4 74-75 3.4496033646051375E-4 0.0 0.0 0.795651016046175 5.51936538336822E-4 76-77 3.4496033646051375E-4 0.0 0.0 0.9237692850076098 5.51936538336822E-4 78-79 3.4496033646051375E-4 0.0 0.0 1.062822796634843 5.51936538336822E-4 80-81 3.4496033646051375E-4 0.0 0.0 1.2540343111349057 5.51936538336822E-4 82-83 3.4496033646051375E-4 0.0 0.0 1.4645291084431111 5.51936538336822E-4 84-85 3.4496033646051375E-4 0.0 0.0 1.6518770671748162 5.51936538336822E-4 86-87 3.4496033646051375E-4 0.0 0.0 1.922222482858921 5.51936538336822E-4 88-89 3.4496033646051375E-4 0.0 0.0 2.2534878939619523 5.51936538336822E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8190 0.0 29.441 1 GGTATCA 5610 0.0 26.534443 1 GTACATG 6665 0.0 25.2599 1 TCAACGC 9645 0.0 24.742842 4 ATCAACG 9645 0.0 24.741985 3 CAACGCA 9715 0.0 24.416916 5 TACATGG 6675 0.0 24.21535 2 TATCAAC 9865 0.0 24.191887 2 AACGCAG 9905 0.0 24.173449 6 ACATGGG 6730 0.0 23.026905 3 GAGTACT 4920 0.0 22.978111 12-13 ACGCAGA 11165 0.0 21.361048 7 AGTACTT 5215 0.0 21.359495 12-13 CGCAGAG 11090 0.0 21.162062 8 GTACTTT 5310 0.0 21.155905 14-15 GTACCTG 1160 0.0 20.909357 1 GCAGAGT 11540 0.0 19.922415 9 AGAGTAC 8940 0.0 19.6589 10-11 TACCTGG 1190 0.0 19.575472 2 TAAGCGT 150 7.680096E-4 19.015514 4 >>END_MODULE