FastQCFastQC Report
Thu 26 May 2016
SRR937333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937333_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449442
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64370.44410193715926544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49840.3438564633838401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.24050634658027023No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22560.15564610381098382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA600.003950263431.6683487
GGTATCA60050.029.3621161
GTATCAA88300.029.2257441
TCAACGC104200.024.5279454
ATCAACG104400.024.526463
CAACGCA104750.024.4898625
TATCAAC105400.024.4740472
AACGCAG106950.024.0263716
GTACATG64750.023.7810861
GTACCGT1401.7805925E-523.7488026
TACATGG65200.023.5343132
ACATGGG64950.022.2351993
GAGTACT54600.022.14174312-13
ACGCAGA121250.020.9990527
AGTACTT56950.020.9361412-13
GTACTTT57850.020.89566214-15
CGCAGAG122550.020.5824878
GTGTAAG6800.019.5693131
AGAGTAC96000.019.39686210-11
GCAGAGT128550.019.1044759
Produced by FastQC (version 0.11.5)