##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937333_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1449442 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05958844852019 31.0 31.0 34.0 30.0 34.0 2 32.27700246025712 33.0 31.0 34.0 31.0 34.0 3 32.36513982622278 34.0 31.0 34.0 30.0 34.0 4 35.99733483644051 37.0 35.0 37.0 35.0 37.0 5 35.8810783736086 37.0 35.0 37.0 35.0 37.0 6 35.96369016490484 37.0 35.0 37.0 35.0 37.0 7 35.91101334168597 37.0 35.0 37.0 35.0 37.0 8 35.8890531666669 37.0 35.0 37.0 35.0 37.0 9 37.56384318931009 39.0 37.0 39.0 35.0 39.0 10-11 37.486152947134144 39.0 37.0 39.0 35.0 39.0 12-13 37.40533943407188 39.0 37.0 39.0 34.0 39.0 14-15 38.5825565976424 40.0 38.0 41.0 34.0 41.0 16-17 38.5629614706901 40.0 38.0 41.0 34.0 41.0 18-19 38.562745525519475 40.0 38.0 41.0 34.0 41.0 20-21 38.451017701984625 40.0 38.0 41.0 34.0 41.0 22-23 38.327699211144704 40.0 38.0 41.0 34.0 41.0 24-25 38.18877436972297 40.0 38.0 41.0 33.5 41.0 26-27 38.141066700150816 40.0 38.0 41.0 33.0 41.0 28-29 38.05056877060275 40.0 37.0 41.0 33.0 41.0 30-31 38.14672818919281 40.0 38.0 41.0 34.0 41.0 32-33 38.0969300599817 40.0 37.0 41.0 33.5 41.0 34-35 37.99708232547422 40.0 37.0 41.0 33.0 41.0 36-37 37.84957038639698 40.0 37.0 41.0 33.0 41.0 38-39 37.730054738306194 40.0 37.0 41.0 33.0 41.0 40-41 37.57942470274767 40.0 37.0 41.0 33.0 41.0 42-43 37.37522439669887 40.0 37.0 41.0 32.5 41.0 44-45 37.10121619216223 40.0 36.0 41.0 31.5 41.0 46-47 36.77600517992441 39.0 36.0 41.0 31.0 41.0 48-49 36.490094118978206 39.0 36.0 41.0 30.0 41.0 50-51 36.2782360384203 39.0 36.0 41.0 30.0 41.0 52-53 36.05002890767619 39.0 35.0 40.0 29.5 41.0 54-55 35.81867953322727 39.0 35.0 40.0 29.0 41.0 56-57 35.57222745028777 38.5 35.0 40.0 28.0 41.0 58-59 35.325906452276115 38.0 34.5 40.0 28.0 41.0 60-61 34.98816924030075 38.0 34.0 40.0 27.5 41.0 62-63 34.612146605383316 37.5 34.0 40.0 26.0 41.0 64-65 34.25392564862892 37.0 34.0 39.5 26.0 41.0 66-67 33.81585258326997 36.0 33.0 39.0 26.0 40.5 68-69 33.41230590806669 36.0 33.0 39.0 25.0 40.0 70-71 33.00564665574752 35.0 32.0 38.5 24.5 40.0 72-73 32.54270160516944 35.0 32.0 37.5 23.5 40.0 74-75 32.090145725044536 35.0 31.0 37.0 22.0 39.0 76-77 30.771243347439913 33.5 29.5 35.5 21.0 38.5 78-79 31.422898605118384 34.0 31.0 36.0 22.0 38.5 80-81 31.285088675504092 34.0 31.0 36.0 21.5 37.5 82-83 30.9586044146644 34.0 31.0 35.0 20.0 37.0 84-85 30.58393781882959 34.0 31.0 35.0 19.0 36.5 86-87 30.21206643660112 34.0 30.5 35.0 18.0 36.0 88-89 29.81125667670731 34.0 30.0 35.0 13.5 36.0 90-91 29.350602507723664 34.0 29.0 35.0 7.0 35.0 92-93 28.967930072400275 34.0 29.0 35.0 2.0 35.0 94-95 28.526180074815 34.0 29.0 35.0 2.0 35.0 96-97 28.00153265877489 33.0 29.0 35.0 2.0 35.0 98-99 27.45620176592095 33.0 27.5 35.0 2.0 35.0 100-101 25.48177988494883 31.5 22.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22291012460274118 1101 2 0.1699036335245978 1101 3 0.21673392055552654 1101 4 0.11188903378541681 1101 5 -0.01985108187495399 1101 6 0.07362855382281452 1101 7 0.15981312687355853 1101 8 0.10249076310574878 1101 9 0.21803866009059192 1101 10-11 0.1606752664727864 1101 12-13 0.06682623313682257 1101 14-15 0.12251510312957237 1101 16-17 0.07080937870706805 1101 18-19 0.058515157676616525 1101 20-21 0.09379748784034803 1101 22-23 0.13612472398722986 1101 24-25 0.26731655917971153 1101 26-27 0.23363742449151204 1101 28-29 0.07541762233707061 1101 30-31 0.1237298429637903 1101 32-33 0.20801430820707623 1101 34-35 0.07225198241916786 1101 36-37 0.1256124633221276 1101 38-39 0.061734254520686704 1101 40-41 -0.08843885117882877 1101 42-43 0.13225509438596816 1101 44-45 0.1780110987578638 1101 46-47 0.25746962560596387 1101 48-49 0.3028924444112917 1101 50-51 0.2622965753276816 1101 52-53 0.3728175142775427 1101 54-55 0.2867410537352697 1101 56-57 0.42713296316576077 1101 58-59 0.36127014053314355 1101 60-61 0.3537082784538512 1101 62-63 0.33425798397446727 1101 64-65 0.32965969650286553 1101 66-67 0.3996220823701364 1101 68-69 0.2988902332474268 1101 70-71 0.22432494976193595 1101 72-73 0.20428674955535797 1101 74-75 0.1991976265273081 1101 76-77 0.1794812766592564 1101 78-79 0.1131695937143391 1101 80-81 0.19040296862526773 1101 82-83 0.2549641244366043 1101 84-85 0.04252185353037419 1101 86-87 0.055661471458059 1101 88-89 0.012301915008738007 1101 90-91 -0.04044363006716978 1101 92-93 0.15277013772151804 1101 94-95 0.18232060722878174 1101 96-97 0.08904803912967552 1101 98-99 0.20291975582970778 1101 100-101 0.4332919547684888 1102 1 0.02287256945034244 1102 2 0.09483892628423973 1102 3 0.13175120633767534 1102 4 0.07415149116337005 1102 5 0.029551536286440694 1102 6 0.07305984099873797 1102 7 -0.006887588361806252 1102 8 0.0625961329539848 1102 9 0.10696157760173719 1102 10-11 0.069348567883047 1102 12-13 0.0578533880970582 1102 14-15 0.031094337140892492 1102 16-17 -0.026541044255147028 1102 18-19 0.04640146681296642 1102 20-21 0.02226371890075285 1102 22-23 0.07700569749145814 1102 24-25 -0.023580409372691236 1102 26-27 0.007448055328318048 1102 28-29 0.02412306463875069 1102 30-31 0.053599904290713596 1102 32-33 0.0380463665202484 1102 34-35 0.059117911602221795 1102 36-37 0.08210485517244592 1102 38-39 0.013566360729242888 1102 40-41 0.14508777510108217 1102 42-43 0.12348636124045953 1102 44-45 0.14583591941046592 1102 46-47 0.1829664504722217 1102 48-49 0.009571445157007474 1102 50-51 -0.04654123631993912 1102 52-53 0.1865154150306907 1102 54-55 0.08212255986921235 1102 56-57 0.054110846914973365 1102 58-59 -0.07723222030724486 1102 60-61 -0.04785934397827418 1102 62-63 -0.27057169479254384 1102 64-65 -0.2932768794509997 1102 66-67 -0.18823202124833216 1102 68-69 -0.13044450559166876 1102 70-71 -0.08641180262215187 1102 72-73 -0.1842457164430371 1102 74-75 -0.14836633302083868 1102 76-77 -0.14091659253464073 1102 78-79 -0.052528513843473235 1102 80-81 0.11898534779084713 1102 82-83 0.16051668603190095 1102 84-85 0.14416419132783886 1102 86-87 -0.04550945223712333 1102 88-89 0.11733875747492206 1102 90-91 0.10790496647955194 1102 92-93 0.1744192828391533 1102 94-95 0.01635878782166955 1102 96-97 -0.06783322447553886 1102 98-99 -0.14838751173854803 1102 100-101 0.14163400968159223 1103 1 0.08112347467169911 1103 2 0.13200692251337642 1103 3 0.07691978819325129 1103 4 0.09386746233911936 1103 5 -0.0292874950017179 1103 6 -0.0825400333058326 1103 7 -0.027268816891812264 1103 8 0.039342835234357665 1103 9 0.05789000976548664 1103 10-11 -0.022241622159874908 1103 12-13 -0.0031492472219269985 1103 14-15 0.04077913442130665 1103 16-17 0.05872652275266432 1103 18-19 0.1205730196514736 1103 20-21 -0.0054762272501847065 1103 22-23 0.0409087905989125 1103 24-25 -0.02210259533051584 1103 26-27 0.05403178205502712 1103 28-29 -0.055912787126338515 1103 30-31 0.0010176130629631075 1103 32-33 0.04329504350441482 1103 34-35 0.006640961716136928 1103 36-37 -0.007881111218310366 1103 38-39 -0.005473461868795937 1103 40-41 0.08821166366066535 1103 42-43 0.16180436354011363 1103 44-45 -0.027377384308259423 1103 46-47 0.07573967734532516 1103 48-49 0.09937850981159357 1103 50-51 0.06990058312143077 1103 52-53 0.14784889358033837 1103 54-55 0.11141102374249101 1103 56-57 -0.09128395100819375 1103 58-59 -2.8911891531180345E-4 1103 60-61 -0.04545506176346237 1103 62-63 -0.051637749170104996 1103 64-65 -0.12258337633360838 1103 66-67 0.07571999309237043 1103 68-69 0.06375654282248888 1103 70-71 -0.14490892091155416 1103 72-73 0.09156773296812304 1103 74-75 0.07715840969632382 1103 76-77 -0.07752342293865055 1103 78-79 -0.019666657407650945 1103 80-81 -0.06975991184877728 1103 82-83 -0.04711913288097591 1103 84-85 -0.001533775405722082 1103 86-87 -0.0066390328000380805 1103 88-89 0.019485221219646576 1103 90-91 0.11682505703739565 1103 92-93 0.025439129862100174 1103 94-95 0.11831795823182034 1103 96-97 0.16763859760829192 1103 98-99 0.04227888830144977 1103 100-101 0.5356222328766798 1104 1 0.10725966267355602 1104 2 0.02456456910463345 1104 3 0.08412552010072005 1104 4 0.05778065941576216 1104 5 -0.04433338411713095 1104 6 -0.060535705671533435 1104 7 -0.027807563202649987 1104 8 -0.04061940836434985 1104 9 -0.02713512436909582 1104 10-11 -0.0047025593723759584 1104 12-13 -0.008634965292742436 1104 14-15 0.06241491394747101 1104 16-17 0.01653024834531891 1104 18-19 -0.0034698224561182656 1104 20-21 -0.12644814178676 1104 22-23 -0.0561091528916009 1104 24-25 -0.058204302096491745 1104 26-27 -0.1549436431644864 1104 28-29 -0.06473586195467362 1104 30-31 0.011589221426781648 1104 32-33 -0.030662423504729475 1104 34-35 0.015223551083984432 1104 36-37 -0.005189771638853813 1104 38-39 -0.1939226158457359 1104 40-41 -0.06789707183109073 1104 42-43 0.04271290914586956 1104 44-45 0.0373899317488835 1104 46-47 -0.07682047161949868 1104 48-49 -0.20985690466242346 1104 50-51 -0.44551698206907986 1104 52-53 -0.28120605559939804 1104 54-55 -0.3312478217801029 1104 56-57 -0.10748258795058518 1104 58-59 -0.22067941559378568 1104 60-61 -0.29439081700189007 1104 62-63 -0.352620666790493 1104 64-65 -0.4364687897036461 1104 66-67 -0.35979659985240886 1104 68-69 -0.3278316180065275 1104 70-71 -0.28561904301266594 1104 72-73 -0.46308649788320366 1104 74-75 -0.5758521665119893 1104 76-77 -0.37357721661059173 1104 78-79 -0.4072869001378905 1104 80-81 -0.5679839626027992 1104 82-83 -0.28885486015169803 1104 84-85 -0.15327310903835922 1104 86-87 -0.29224026485102783 1104 88-89 -0.24334459529748997 1104 90-91 -0.14778254870628516 1104 92-93 -0.507674264460455 1104 94-95 -0.32213051863226383 1104 96-97 -0.2531391989781753 1104 98-99 0.09620651082575549 1104 100-101 0.24353727729381447 1105 1 0.004596743942293813 1105 2 -0.04477327274250342 1105 3 0.022753920393597582 1105 4 0.035594012077218906 1105 5 0.031153632972937828 1105 6 0.02278962396104589 1105 7 -0.06746729380391514 1105 8 -0.13146886478021713 1105 9 0.06772681082075849 1105 10-11 0.045558413118094165 1105 12-13 -0.02358017526253775 1105 14-15 -0.015009711605706855 1105 16-17 0.026824886928650926 1105 18-19 -0.005300110041076778 1105 20-21 0.010485327434508918 1105 22-23 -0.08184147206855386 1105 24-25 -0.08819912658602647 1105 26-27 0.04487791643339989 1105 28-29 -0.16235178308471632 1105 30-31 -0.04522240749028583 1105 32-33 0.0015441442997001786 1105 34-35 -0.11413718588381272 1105 36-37 -0.17741839466144427 1105 38-39 -0.14113124257788456 1105 40-41 -0.03823645855448632 1105 42-43 -0.10005574398099526 1105 44-45 -0.06690788057723296 1105 46-47 -0.10947732049964998 1105 48-49 -0.019043299641360534 1105 50-51 -0.02197474760951934 1105 52-53 -0.05165855697298838 1105 54-55 -0.008376848305850615 1105 56-57 -0.07878922353049234 1105 58-59 -0.1787914158177557 1105 60-61 -0.08886958554924718 1105 62-63 -0.1559497137694308 1105 64-65 -0.10733159901428024 1105 66-67 -0.11153871711446328 1105 68-69 -0.10578802628781148 1105 70-71 -0.11673467076008848 1105 72-73 -0.17391641878850095 1105 74-75 -0.06939930429138741 1105 76-77 -0.17109343820020584 1105 78-79 -0.006134872725962026 1105 80-81 0.01561200228771753 1105 82-83 -0.22564577925002993 1105 84-85 -0.15345469907493836 1105 86-87 -0.13080808328587779 1105 88-89 -0.18095383609171023 1105 90-91 -0.1762581606199909 1105 92-93 -0.27292773032211315 1105 94-95 -0.16904428514013858 1105 96-97 -0.10108748431328252 1105 98-99 -0.048610960079983556 1105 100-101 0.19385062703894462 1106 1 -0.13536005076971236 1106 2 -0.020129634540275276 1106 3 -0.08037760163670526 1106 4 -0.004075867135746591 1106 5 -0.12314764466668748 1106 6 -0.1397607972818946 1106 7 -0.05149399908200536 1106 8 -0.022240711401444457 1106 9 -0.2937272848527712 1106 10-11 -0.3116167138663499 1106 12-13 -0.34964809783411965 1106 14-15 -0.22570805927907145 1106 16-17 -0.15190490689013814 1106 18-19 -0.15230102048743532 1106 20-21 -0.14256354811686833 1106 22-23 -0.11217540002012072 1106 24-25 -0.07730133344943368 1106 26-27 -0.07863528676151788 1106 28-29 0.13115863795150773 1106 30-31 0.0684748574668319 1106 32-33 -0.0028879292526511335 1106 34-35 -0.18792145574803953 1106 36-37 -0.04028854881714494 1106 38-39 0.0991505823459562 1106 40-41 -0.23330389790903183 1106 42-43 -0.36774183206775746 1106 44-45 -0.25940699123393784 1106 46-47 -0.07013475407453029 1106 48-49 0.019974968718948105 1106 50-51 -0.1279995424145426 1106 52-53 -0.07692775020726828 1106 54-55 -0.06709460907293163 1106 56-57 -0.061179364869317965 1106 58-59 -0.2318671224042319 1106 60-61 -0.036215940189208595 1106 62-63 0.09234223028764887 1106 64-65 0.11301300105687062 1106 66-67 -0.08641746064679978 1106 68-69 0.1861773491873322 1106 70-71 0.1011542220527275 1106 72-73 0.14272812555334724 1106 74-75 -0.06055862895897235 1106 76-77 -0.018600994388187075 1106 78-79 -0.08462663262652725 1106 80-81 0.07091537386783742 1106 82-83 -0.07113196363566132 1106 84-85 -0.29431504935130093 1106 86-87 -0.15348310733906345 1106 88-89 -0.20589097911620158 1106 90-91 -0.3514811855241895 1106 92-93 -0.28280956179220595 1106 94-95 -0.49763122415401284 1106 96-97 -0.1478439007206127 1106 98-99 0.10777198382291076 1106 100-101 0.4733453852969163 1107 1 0.018673157940860108 1107 2 -0.04337346501612416 1107 3 -0.08545289842952997 1107 4 0.03461705866256182 1107 5 -0.06926544805103418 1107 6 -0.0921972187486304 1107 7 0.0033158930821102217 1107 8 -0.06498271438680803 1107 9 0.034607187755796076 1107 10-11 -0.12665567010423473 1107 12-13 -0.10375864357020248 1107 14-15 -0.1490707289497024 1107 16-17 -0.11048382378814381 1107 18-19 -0.2669852029295754 1107 20-21 -0.20179656012808778 1107 22-23 -0.22879605973023587 1107 24-25 -0.3655482853069145 1107 26-27 -0.18672402467705496 1107 28-29 -0.1532593900328152 1107 30-31 -0.11162755523911017 1107 32-33 -0.14780155940206186 1107 34-35 -0.058832271422609494 1107 36-37 -0.12303916611832477 1107 38-39 -0.013837329711300583 1107 40-41 -0.03472648066924222 1107 42-43 -0.002776899657007448 1107 44-45 0.030713225553867574 1107 46-47 -0.1021380707605033 1107 48-49 0.07812726772986167 1107 50-51 -0.18638202152395422 1107 52-53 -0.1143429148906634 1107 54-55 0.048355968469856236 1107 56-57 -0.014366487339088962 1107 58-59 -0.0832710466261588 1107 60-61 -0.01728231924700907 1107 62-63 -0.17446876395087685 1107 64-65 0.01455564366390405 1107 66-67 0.10030001420776813 1107 68-69 0.025398730128415536 1107 70-71 -0.11123530893788569 1107 72-73 -0.22242121250449287 1107 74-75 -0.26733839194321973 1107 76-77 -0.3631051829684644 1107 78-79 -0.25114100841995324 1107 80-81 -0.06599252270457256 1107 82-83 0.05556997406226216 1107 84-85 -0.03257106890963257 1107 86-87 -0.06314831736096238 1107 88-89 0.11496482002725017 1107 90-91 0.2951064817051403 1107 92-93 0.20725738225310408 1107 94-95 0.09739043370070988 1107 96-97 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-0.4343286475338921 2316 78-79 -0.350308262645612 2316 80-81 -0.5404712228970361 2316 82-83 -0.5678722006047359 2316 84-85 -0.6039862841467851 2316 86-87 -0.7763041999380924 2316 88-89 -0.6907089751592146 2316 90-91 -0.6015512207168747 2316 92-93 -0.47757180481932693 2316 94-95 -0.45004106009657363 2316 96-97 -0.3382908503127844 2316 98-99 -0.2554161432109261 2316 100-101 -0.978449766766996 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 8.0 5 29.0 6 53.0 7 148.0 8 346.0 9 613.0 10 1082.0 11 1764.0 12 2716.0 13 3701.0 14 4817.0 15 6999.0 16 9449.0 17 13006.0 18 11585.0 19 7348.0 20 6468.0 21 6552.0 22 6927.0 23 7734.0 24 9085.0 25 10738.0 26 13180.0 27 16960.0 28 21381.0 29 26938.0 30 34058.0 31 42913.0 32 55316.0 33 72325.0 34 98222.0 35 138615.0 36 212672.0 37 322500.0 38 259061.0 39 24126.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65846499632761 20.192551951888934 15.308135585732352 21.840847466051105 2 15.739643255818446 21.182427444492433 39.55211729755313 23.525812002135996 3 19.72910954698429 25.152437972681902 29.75862435337185 25.359828126961965 4 13.132295048715298 17.150117079538195 37.14450112526062 32.57308674648589 5 13.50687205900924 38.12345208742631 33.961848807111025 14.407827046453422 6 31.824453824299283 37.00210149836972 17.138250443963955 14.035194233367049 7 28.734644090622457 33.109707045883866 20.250965543981753 17.90468331951192 8 26.793690261493737 35.363677884316864 19.125083997841926 18.717547856347476 9 26.39243239812286 17.12810861007201 20.22392065360325 36.25553833820187 10-11 24.889371220097114 27.456566044036258 26.894073719403742 20.75998901646289 12-13 26.030847459965916 24.484127805490587 27.851820421004696 21.633204313538798 14-15 22.792840279224695 25.7499782674988 26.36383518623029 25.09334626704621 16-17 21.886008546737294 29.41231867159914 26.78665307062994 21.915019711033626 18-19 21.85127104085572 27.682446072350604 29.47968942530988 20.9865934614838 20-21 22.593936149221562 26.535211481383868 29.710605874536544 21.160246494858022 22-23 22.323603386307195 26.48556272812918 29.628359650082047 21.562474235481574 24-25 22.225729325025295 26.755721780729726 29.63635253489366 21.382196359351322 26-27 22.039310166159396 26.945025998303464 29.627958464808202 21.387705370728945 28-29 21.84791330463634 27.111205556517643 29.698177931926175 21.342703206919843 30-31 21.973352503929096 26.755710128449433 29.696393508674372 21.5745438589471 32-33 21.812118042667457 26.926189526728216 29.603944138502953 21.657748292101374 34-35 22.23855801059994 26.882345067964085 29.46271737675602 21.416379544679952 36-37 22.239420411441095 26.577952067071326 29.520912185516906 21.66171533597067 38-39 22.030106078789657 26.93578871586056 29.584740053323987 21.4493651520258 40-41 22.211858080557896 27.00273622538881 29.36985405418085 21.415551639872447 42-43 22.011351549219775 26.974161097327926 29.222148734896713 21.792338618555583 44-45 22.13224123490281 27.063035292202102 29.057526965549503 21.747196507345585 46-47 22.33221474194897 27.395680544650975 28.580481316258254 21.691623397141797 48-49 22.420835052385677 27.48040280328568 28.31251612689573 21.786246017432916 50-51 22.285990056863263 27.64853647127653 28.26611896164179 21.799354510218414 52-53 22.610597733472606 27.734845547458953 27.94603026544008 21.708526453628362 54-55 22.36036350540415 27.558398335359403 28.15328243558556 21.927955723650893 56-57 22.39399713820905 27.848130521952584 28.021576579124936 21.736295760713435 58-59 22.642058116157806 27.500134534531217 27.99487664908289 21.862930700228087 60-61 22.383406855879713 27.546842164087977 28.491998989956137 21.57775199007618 62-63 22.317657415750336 27.554638267691985 28.48723853731298 21.640465779244703 64-65 22.192471309648816 27.639222542192098 28.27784761308145 21.890458535077638 66-67 22.254253705908894 27.72922269397465 28.02885524222425 21.98766835789221 68-69 22.161114415064556 27.622905918277517 28.20633733533318 22.009642331324745 70-71 22.320900042913067 27.622974910344812 28.102780242327736 21.95334480441439 72-73 22.377124958604703 27.511142234242193 28.01499199690915 22.096740810243958 74-75 22.249113796895635 27.972937171684 27.763787719687986 22.014161311732376 76-77 22.35267088990108 27.63146093462174 27.91143074369309 22.104437431784092 78-79 22.368504569344616 27.680997238937465 27.826156548520053 22.124341643197866 80-81 22.317665114459604 27.907438830749637 27.758933354674888 22.01596270011587 82-83 22.425657392727388 27.826957758800308 27.85672791799304 21.89065693047926 84-85 22.23971194054462 27.752629839598242 27.81579290517675 22.191865314680392 86-87 22.19899106000792 27.725152162004413 27.869207598510325 22.206649179477346 88-89 22.364710005643552 27.66330077367704 27.764374152259975 22.207615068419432 90-91 22.248012646200998 27.65587125825146 27.84972493408837 22.24639116145917 92-93 22.268938146054722 27.519408592042694 27.932814620995604 22.27883864090698 94-95 22.215618148225317 27.52100463488708 27.8572029788015 22.406174238086106 96-97 22.13524238983002 27.41799947841997 27.914155930351125 22.532602201398884 98-99 22.002451294070084 27.45118195770293 27.75015980326175 22.79620694496524 100-101 21.81495947401959 27.559943709028534 27.976640419856952 22.64845639709492 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 173.0 1 130.0 2 120.0 3 183.5 4 176.5 5 176.5 6 340.0 7 785.0 8 1332.0 9 1672.0 10 1640.5 11 1515.5 12 1600.5 13 1746.0 14 1920.0 15 2034.0 16 2101.5 17 2243.0 18 2377.0 19 2489.0 20 2681.0 21 3059.5 22 3515.5 23 4113.0 24 4938.5 25 6018.5 26 7664.5 27 9667.5 28 11475.5 29 13633.0 30 16574.0 31 19477.0 32 22933.5 33 26285.0 34 29950.0 35 33283.0 36 36925.0 37 40882.0 38 43526.0 39 45276.5 40 45838.0 41 47386.5 42 49215.5 43 51265.5 44 53698.0 45 55234.5 46 55745.0 47 57695.0 48 62251.0 49 61416.0 50 56097.5 51 51958.5 52 48664.5 53 46843.0 54 45047.0 55 41600.0 56 36765.0 57 32437.5 58 29117.0 59 25916.0 60 21860.5 61 17220.0 62 13303.0 63 10024.0 64 7431.5 65 5571.0 66 3985.0 67 2993.0 68 2113.5 69 1312.5 70 891.0 71 594.5 72 421.0 73 319.0 74 242.5 75 175.0 76 102.0 77 59.0 78 36.5 79 20.5 80 11.0 81 6.5 82 5.0 83 3.0 84 1.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.054779701429929585 2 0.0 3 0.0 4 0.0 5 0.021594517062428164 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035875874991893432 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011383691103196954 24-25 1.7248016823025688E-4 26-27 0.0014143373794881064 28-29 0.0031046430281446236 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.4496033646051375E-5 40-41 0.0 42-43 0.014660814299571836 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.379841345842055E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.4496033646051375E-5 82-83 2.75968269168411E-4 84-85 0.0013108492785499524 86-87 0.0 88-89 0.0 90-91 0.01055578629569172 92-93 0.0013453453121960038 94-95 0.0 96-97 0.0 98-99 2.4147223552235963E-4 100-101 0.012867020549977163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1449442.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.98828976381415 #Duplication Level Percentage of deduplicated Percentage of total 1 78.75496844394078 45.66865930467278 2 12.16646525092539 14.110250247440748 3 3.6769927860331015 6.396675694078254 4 1.6918173598381172 3.9242238115897514 5 0.9093258439957874 2.6365125265676292 6 0.5708666120803975 1.9862147110682988 7 0.41382496304175775 1.6797901307855596 8 0.2666213410007939 1.2368732463336591 9 0.22329989674050796 1.165390120477652 >10 1.2070119611529027 12.770513997951202 >50 0.0816275760510461 3.2106888651993466 >100 0.03562891972454337 3.554213663526902 >500 9.532587537459207E-4 0.3830754646865279 >1k 4.7662937687296036E-4 0.8321388566413882 >5k 1.1915734421824009E-4 0.4447793589803716 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6437 0.44410193715926544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4984 0.3438564633838401 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3486 0.24050634658027023 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2256 0.15564610381098382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 6.899206729210275E-5 5 0.0 0.0 0.0 0.0 6.899206729210275E-5 6 0.0 0.0 0.0 0.0 6.899206729210275E-5 7 0.0 0.0 0.0 0.0 6.899206729210275E-5 8 0.0 0.0 0.0 0.0 6.899206729210275E-5 9 0.0 0.0 0.0 0.0 6.899206729210275E-5 10-11 0.0 0.0 0.0 0.0 6.899206729210275E-5 12-13 0.0 0.0 0.0 0.0 6.899206729210275E-5 14-15 6.899206729210275E-5 0.0 0.0 0.0 6.899206729210275E-5 16-17 6.899206729210275E-5 0.0 0.0 0.0 6.899206729210275E-5 18-19 1.0348810093815413E-4 0.0 0.0 0.0 6.899206729210275E-5 20-21 1.379841345842055E-4 0.0 0.0 0.0 6.899206729210275E-5 22-23 1.379841345842055E-4 0.0 0.0 2.0697620187630825E-4 1.0348810093815413E-4 24-25 1.379841345842055E-4 0.0 0.0 2.75968269168411E-4 1.379841345842055E-4 26-27 1.379841345842055E-4 0.0 0.0 3.794563701065651E-4 1.379841345842055E-4 28-29 2.0697620187630825E-4 0.0 0.0 0.0010693770430275926 1.379841345842055E-4 30-31 2.0697620187630825E-4 0.0 0.0 0.0031391390617906754 1.379841345842055E-4 32-33 2.4147223552235963E-4 0.0 0.0 0.006105797955351094 1.379841345842055E-4 34-35 2.75968269168411E-4 0.0 0.0 0.008830984613389152 1.379841345842055E-4 36-37 3.4496033646051375E-4 0.0 0.0 0.011349195069550903 1.379841345842055E-4 38-39 4.139524037526165E-4 0.0 0.0 0.01452283016498763 1.379841345842055E-4 40-41 4.139524037526165E-4 0.0 0.0 0.01955925107731113 1.379841345842055E-4 42-43 4.484484373986679E-4 0.0 0.0 0.024940632326095145 1.379841345842055E-4 44-45 4.8294447104471925E-4 0.0 0.0 0.030977438214154135 1.379841345842055E-4 46-47 5.174405046907706E-4 0.0 0.0 0.03835958941440913 2.4147223552235963E-4 48-49 5.51936538336822E-4 0.0 0.0 0.04591422078289438 2.75968269168411E-4 50-51 5.51936538336822E-4 0.0 0.0 0.05712543171786108 2.75968269168411E-4 52-53 5.51936538336822E-4 0.0 0.0 0.08986216764796384 2.75968269168411E-4 54-55 5.51936538336822E-4 0.0 0.0 0.13294771367188202 2.75968269168411E-4 56-57 6.209286056289248E-4 0.0 0.0 0.16306275104488485 2.75968269168411E-4 58-59 6.209286056289248E-4 0.0 0.0 0.21180564658675544 2.75968269168411E-4 60-61 6.209286056289248E-4 0.0 0.0 0.2713113046261941 2.75968269168411E-4 62-63 6.209286056289248E-4 0.0 0.0 0.3063247787769362 2.75968269168411E-4 64-65 6.209286056289248E-4 0.0 0.0 0.33709524078921405 2.75968269168411E-4 66-67 6.899206729210275E-4 0.0 0.0 0.4307864681718896 2.75968269168411E-4 68-69 6.899206729210275E-4 0.0 0.0 0.5177509689935851 2.75968269168411E-4 70-71 6.899206729210275E-4 0.0 0.0 0.5607330269165651 3.4496033646051375E-4 72-73 6.899206729210275E-4 0.0 0.0 0.6601850919181312 5.51936538336822E-4 74-75 6.899206729210275E-4 0.0 0.0 0.8022397584725709 5.51936538336822E-4 76-77 6.899206729210275E-4 0.0 0.0 0.9317033727462016 5.51936538336822E-4 78-79 6.899206729210275E-4 0.0 0.0 1.0730681186277202 5.51936538336822E-4 80-81 6.899206729210275E-4 0.0 0.0 1.2671772999540514 5.51936538336822E-4 82-83 6.899206729210275E-4 0.0 0.0 1.481811621299783 5.51936538336822E-4 84-85 7.934087738591816E-4 0.0 0.0 1.671919262723172 5.51936538336822E-4 86-87 8.968968747973358E-4 0.0 0.0 1.9460592421083422 5.51936538336822E-4 88-89 8.968968747973358E-4 0.0 0.0 2.2804982883068106 5.51936538336822E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGTA 60 0.0039502634 31.668348 7 GGTATCA 6005 0.0 29.362116 1 GTATCAA 8830 0.0 29.225744 1 TCAACGC 10420 0.0 24.527945 4 ATCAACG 10440 0.0 24.52646 3 CAACGCA 10475 0.0 24.489862 5 TATCAAC 10540 0.0 24.474047 2 AACGCAG 10695 0.0 24.026371 6 GTACATG 6475 0.0 23.781086 1 GTACCGT 140 1.7805925E-5 23.748802 6 TACATGG 6520 0.0 23.534313 2 ACATGGG 6495 0.0 22.235199 3 GAGTACT 5460 0.0 22.141743 12-13 ACGCAGA 12125 0.0 20.999052 7 AGTACTT 5695 0.0 20.93614 12-13 GTACTTT 5785 0.0 20.895662 14-15 CGCAGAG 12255 0.0 20.582487 8 GTGTAAG 680 0.0 19.569313 1 AGAGTAC 9600 0.0 19.396862 10-11 GCAGAGT 12855 0.0 19.104475 9 >>END_MODULE