FastQCFastQC Report
Thu 26 May 2016
SRR937332_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937332_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512650
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56840.37576438700294185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.27051862625194195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.1959475093379169No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24470.16176908075232208No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17610.11641820645886358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77050.031.5804791
GGTATCA51950.027.810541
GTACATG72900.026.337541
ATCAACG93100.025.8774853
TACATGG72600.025.790052
TATCAAC94050.025.5672682
TCAACGC94700.025.4402734
CAACGCA95800.024.9985285
AACGCAG98250.024.5637236
ACATGGG74350.023.9670243
TACCTGG11950.023.0649592
GTACCTG14000.022.065141
ACGCAGA111100.021.7212037
CGCAGAG111650.021.4433028
TCGCGCA1856.5527947E-620.54119
GAGTACT48250.020.18189612-13
AGTACTT50900.020.06439612-13
CATGGGG31050.020.0481134
GCAGAGT116900.019.910729
CATGGGA43250.019.8864044
Produced by FastQC (version 0.11.5)