FastQCFastQC Report
Thu 26 May 2016
SRR937332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937332_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512650
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68070.4500049581859651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52030.3439658876805606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37500.24790929825141308No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24180.15985191551251116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95150.030.2748411
GGACCGA3050.028.031486
GGTATCA65050.025.2840611
ATCAACG114800.024.9081973
TCAACGC115150.024.873744
TATCAAC115450.024.8091052
CAACGCA115650.024.76625
AACGCAG118500.024.209846
GTACATG70750.023.8517321
AGGACCG4250.023.470275
TACATGG71650.023.2690492
ACATGGG70650.022.8588543
CATGGGG29150.022.486834
AGTACTT60800.022.30512212-13
ACGCAGA133300.021.4163957
GAGTACT59200.021.3433212-13
CGCAGAG134250.021.1940828
TAACGCA1150.00372410820.6518944
GTACTTT61850.020.31231114-15
AGAGTAC104350.020.27950510-11
Produced by FastQC (version 0.11.5)