##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937332_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1512650 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15086173272072 33.0 31.0 34.0 31.0 34.0 2 32.35092519750108 34.0 31.0 34.0 31.0 34.0 3 32.41307837239282 34.0 31.0 34.0 31.0 34.0 4 36.06495025286748 37.0 35.0 37.0 35.0 37.0 5 35.98369153472383 37.0 35.0 37.0 35.0 37.0 6 36.024893399001755 37.0 35.0 37.0 35.0 37.0 7 35.965096354080586 37.0 35.0 37.0 35.0 37.0 8 35.93829107857072 37.0 35.0 37.0 35.0 37.0 9 37.63560109741183 39.0 37.0 39.0 35.0 39.0 10-11 37.55310613823423 39.0 37.0 39.0 35.0 39.0 12-13 37.456336561663306 39.0 37.0 39.0 34.5 39.0 14-15 38.63959342875087 40.0 38.0 41.0 34.0 41.0 16-17 38.612819555085444 40.0 38.0 41.0 34.0 41.0 18-19 38.604684163554026 40.0 38.0 41.0 34.0 41.0 20-21 38.50977225399134 40.0 38.0 41.0 34.0 41.0 22-23 38.358811027005586 40.0 38.0 41.0 34.0 41.0 24-25 38.302883680957265 40.0 38.0 41.0 34.0 41.0 26-27 38.2606237397944 40.0 38.0 41.0 34.0 41.0 28-29 38.14388391233928 40.0 38.0 41.0 33.5 41.0 30-31 38.230169239414266 40.0 38.0 41.0 34.0 41.0 32-33 38.19213763924239 40.0 38.0 41.0 34.0 41.0 34-35 38.07894489802664 40.0 37.0 41.0 33.5 41.0 36-37 37.91987273989356 40.0 37.0 41.0 33.0 41.0 38-39 37.81110964201898 40.0 37.0 41.0 33.0 41.0 40-41 37.647984993223815 40.0 37.0 41.0 33.0 41.0 42-43 37.44604468978283 40.0 37.0 41.0 32.5 41.0 44-45 37.175901563481304 40.0 36.5 41.0 31.5 41.0 46-47 36.85602287376459 40.0 36.0 41.0 31.0 41.0 48-49 36.555214358906554 39.0 36.0 41.0 30.0 41.0 50-51 36.326521997818396 39.0 36.0 41.0 30.0 41.0 52-53 36.086963937460744 39.0 35.0 40.5 29.5 41.0 54-55 35.863501801474236 39.0 35.0 40.0 29.0 41.0 56-57 35.59578091428949 38.5 35.0 40.0 28.0 41.0 58-59 35.327205566390106 38.0 34.5 40.0 28.0 41.0 60-61 35.00013585429544 38.0 34.0 40.0 27.5 41.0 62-63 34.62685353518659 37.5 34.0 40.0 26.5 41.0 64-65 34.227016163686244 37.0 33.5 39.5 26.0 41.0 66-67 33.813070439295274 36.0 33.0 39.0 26.0 40.5 68-69 33.37687799557069 36.0 33.0 39.0 25.0 40.0 70-71 32.96300300796615 35.0 32.0 38.5 24.5 40.0 72-73 32.48364063068126 35.0 32.0 37.5 23.0 39.5 74-75 32.00737282253 35.0 31.0 37.0 22.0 39.0 76-77 30.739560704723498 33.5 29.5 35.5 20.5 38.0 78-79 31.34924040591016 34.0 31.0 36.0 21.5 38.5 80-81 31.167797243248604 34.0 31.0 36.0 20.5 37.5 82-83 30.82070307076984 34.0 31.0 35.0 20.0 37.0 84-85 30.451130135854292 34.0 30.5 35.0 18.5 36.5 86-87 30.04404819356758 34.0 30.0 35.0 16.0 36.0 88-89 29.58174164545665 34.0 29.5 35.0 9.0 36.0 90-91 29.06144184047863 34.0 29.0 35.0 4.5 35.0 92-93 28.698553201335407 34.0 29.0 35.0 2.0 35.0 94-95 28.246487951608106 33.5 29.0 35.0 2.0 35.0 96-97 27.746071133441312 33.0 27.5 35.0 2.0 35.0 98-99 26.97240934783327 33.0 26.5 35.0 2.0 35.0 100-101 24.455730010246917 30.5 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0011872818826290654 1101 2 -0.020293723053342205 1101 3 0.005072532888448222 1101 4 0.0201848566731897 1101 5 0.09833611231994865 1101 6 0.09474494492588548 1101 7 -0.04463861717022155 1101 8 0.008318110403266132 1101 9 0.08986140304154588 1101 10-11 0.01605993845463871 1101 12-13 0.012859468581801536 1101 14-15 -0.002419028401710932 1101 16-17 0.08847235757680494 1101 18-19 0.10456302887577351 1101 20-21 -0.005828950911222819 1101 22-23 0.11979478139439692 1101 24-25 0.0790238075267169 1101 26-27 0.01878215202642508 1101 28-29 -0.10416491857236565 1101 30-31 0.019624184846733783 1101 32-33 0.0948167174658181 1101 34-35 0.14078502951980454 1101 36-37 0.09781657417482137 1101 38-39 0.174106597948402 1101 40-41 0.29294634685994936 1101 42-43 0.2622926916718171 1101 44-45 0.17161682975878279 1101 46-47 0.17552727426490122 1101 48-49 0.2023739702793037 1101 50-51 0.261252289072047 1101 52-53 0.20113647051699957 1101 54-55 0.1873137518368253 1101 56-57 0.08285107732190511 1101 58-59 0.14001578888800026 1101 60-61 0.21684371966443905 1101 62-63 0.22550442964845985 1101 64-65 0.29642297441501597 1101 66-67 0.32262220466225955 1101 68-69 0.25830024522846173 1101 70-71 0.31370848364431225 1101 72-73 0.35841508671430944 1101 74-75 0.30588552545305703 1101 76-77 0.4213138978665576 1101 78-79 0.17432872908153385 1101 80-81 0.12422097570806656 1101 82-83 0.07712991788770296 1101 84-85 0.16939310479655134 1101 86-87 0.10201524679742491 1101 88-89 0.12344850594152845 1101 90-91 0.07443160669208737 1101 92-93 0.15692436190544257 1101 94-95 0.2658213239193401 1101 96-97 0.11010425857215722 1101 98-99 0.28223304189902976 1101 100-101 1.1269736939505215 1102 1 -0.022925014715561076 1102 2 0.025397392999536805 1102 3 0.016190522101354077 1102 4 0.013256173010354644 1102 5 -0.03523260903503456 1102 6 0.04237547569614719 1102 7 -0.0944016652737929 1102 8 -0.04586998493982719 1102 9 -0.06534681389916841 1102 10-11 -0.03350052431524375 1102 12-13 -0.11791599558231525 1102 14-15 -0.03433500489119723 1102 16-17 -0.06702995623948027 1102 18-19 -0.13958926755000078 1102 20-21 -0.03590209714766246 1102 22-23 0.01442918781107494 1102 24-25 -0.1327279322701571 1102 26-27 -0.17258141305104147 1102 28-29 -0.12972813729854948 1102 30-31 -0.054428877921893104 1102 32-33 -0.03512420122385862 1102 34-35 -0.11648906047915375 1102 36-37 -0.12169706850509954 1102 38-39 -0.02049667465022509 1102 40-41 -0.13418460383555697 1102 42-43 -0.061307040822711656 1102 44-45 0.022042043443200043 1102 46-47 -0.06686359076121562 1102 48-49 0.13178773606877314 1102 50-51 -0.06593496111554487 1102 52-53 -0.07557934602623106 1102 54-55 0.08284520345855384 1102 56-57 -0.032189872435274935 1102 58-59 -0.09707171703455231 1102 60-61 0.04778045634296291 1102 62-63 -0.11583660568527421 1102 64-65 -0.05285635561440216 1102 66-67 -0.22788072752005917 1102 68-69 -0.21595251827090323 1102 70-71 -0.10589978727495009 1102 72-73 -0.23817203011840604 1102 74-75 -0.20588166520560947 1102 76-77 0.04734485330342153 1102 78-79 -0.06367041084260094 1102 80-81 -0.21576976703740414 1102 82-83 0.02108854803949356 1102 84-85 -0.03811810288060258 1102 86-87 -0.19133196357600823 1102 88-89 0.14064073952938116 1102 90-91 0.07248881002642804 1102 92-93 -0.041570803732106754 1102 94-95 -0.0705631677920735 1102 96-97 0.1751553707523783 1102 98-99 0.4574536062666219 1102 100-101 1.0848125693134598 1103 1 -0.09912422047946734 1103 2 0.021082901032464463 1103 3 -0.02926219546027653 1103 4 0.005652803173447296 1103 5 -0.11336107268439122 1103 6 -0.11687620488521588 1103 7 -0.15784949006460636 1103 8 -0.10659491397603915 1103 9 -0.020865538335669953 1103 10-11 -0.13415743579437844 1103 12-13 -0.1299372164220287 1103 14-15 -0.2475278053859924 1103 16-17 -0.2615295605837531 1103 18-19 -0.3083998310428626 1103 20-21 -0.2409582815907001 1103 22-23 -0.148646777092047 1103 24-25 -0.11280140691844309 1103 26-27 -0.2400931423075363 1103 28-29 -0.2705855653069804 1103 30-31 -0.22754030727100627 1103 32-33 -0.054592091626055606 1103 34-35 -0.20917403244093435 1103 36-37 -0.23941436924566517 1103 38-39 -0.15436889297109957 1103 40-41 -0.1701478984164524 1103 42-43 -0.14716632829675547 1103 44-45 -0.27721476853209026 1103 46-47 -0.05890909931259358 1103 48-49 -0.17134038692781672 1103 50-51 -0.09343909963126862 1103 52-53 -0.018281051516787272 1103 54-55 -0.28019260833427495 1103 56-57 -0.28133416929306065 1103 58-59 -0.1816827198199249 1103 60-61 -0.03420668924937331 1103 62-63 0.09425126943574469 1103 64-65 -0.1955074450884311 1103 66-67 0.011641374862549014 1103 68-69 -0.20573044257552908 1103 70-71 -0.21123261500202517 1103 72-73 -0.13188869807567727 1103 74-75 0.09537552630671797 1103 76-77 0.26604260256902634 1103 78-79 0.06374452649889051 1103 80-81 -0.0378077745809513 1103 82-83 -0.07784881472313288 1103 84-85 -0.20665847056624997 1103 86-87 -0.1635901989813604 1103 88-89 -0.09761396837499348 1103 90-91 -0.15915075341957063 1103 92-93 -0.1482539458069141 1103 94-95 -0.06342989877840921 1103 96-97 0.15249232353524533 1103 98-99 0.31500956720820383 1103 100-101 0.8146850588271697 1104 1 -0.08437571935851906 1104 2 -0.04726523627515178 1104 3 -0.11984940826356905 1104 4 -0.04636177245519946 1104 5 -0.09984543417873226 1104 6 -0.13515551585426522 1104 7 -0.07084199405613134 1104 8 -0.05182083180489627 1104 9 -0.006827546468485934 1104 10-11 -0.04862852584531652 1104 12-13 -0.05878694880932045 1104 14-15 -0.161219610789324 1104 16-17 -0.2850709533517062 1104 18-19 -0.1984206742325867 1104 20-21 -0.17496599869932794 1104 22-23 -0.1989492699538431 1104 24-25 -0.1598205115341429 1104 26-27 -0.46580039958897856 1104 28-29 -0.29336567390078727 1104 30-31 -0.2406218966245035 1104 32-33 -0.20215861441347016 1104 34-35 -0.2806787056015878 1104 36-37 -0.3319784076281138 1104 38-39 -0.3448784320333189 1104 40-41 -0.34627556864234066 1104 42-43 -0.29842192924596134 1104 44-45 -0.289008305139447 1104 46-47 -0.43898196387058164 1104 48-49 -0.2949975261363491 1104 50-51 -0.3849363892014921 1104 52-53 -0.4134464639806552 1104 54-55 -0.306079326939674 1104 56-57 -0.37047811772068684 1104 58-59 -0.41769151220213274 1104 60-61 -0.44428736193725626 1104 62-63 -0.36898870710250975 1104 64-65 -0.2261255711576311 1104 66-67 -0.34144402515721595 1104 68-69 -0.15173377707832714 1104 70-71 -0.14788300539154164 1104 72-73 -0.13642277959473148 1104 74-75 -0.10010197361091855 1104 76-77 -0.24989528832569263 1104 78-79 -0.5855563249234983 1104 80-81 -0.31755550983270453 1104 82-83 -0.38921947605713214 1104 84-85 -0.29997377399140035 1104 86-87 -0.13227362057928005 1104 88-89 -0.132963162276436 1104 90-91 -0.20936918746668454 1104 92-93 -0.026725617307103988 1104 94-95 0.053319134205587204 1104 96-97 0.10267210750918565 1104 98-99 0.32412290124571186 1104 100-101 1.0559049882721077 1105 1 0.09063021969537033 1105 2 0.04423504883352081 1105 3 0.06955170674480371 1105 4 0.06198940644273421 1105 5 -0.05416465159377282 1105 6 -0.08591709513475365 1105 7 -0.06759383431074895 1105 8 -0.041648263519412865 1105 9 0.028542629562686272 1105 10-11 -0.05292901436444453 1105 12-13 0.0611294511253746 1105 14-15 0.10177498799478002 1105 16-17 0.13018343413500588 1105 18-19 0.03172797423643914 1105 20-21 -0.055422472506656106 1105 22-23 -0.10566624617274556 1105 24-25 -0.07988203910992553 1105 26-27 -0.002524884408011019 1105 28-29 -0.03852504675032975 1105 30-31 -0.0012447934581132358 1105 32-33 -9.386969569433745E-4 1105 34-35 -0.05307268795108655 1105 36-37 0.03822506077936083 1105 38-39 -0.0879723433868449 1105 40-41 -0.008142139148034744 1105 42-43 -0.1080127940437734 1105 44-45 -0.019116905468898437 1105 46-47 -0.27220958147662344 1105 48-49 -0.20421299501143864 1105 50-51 -0.1088313928637632 1105 52-53 -0.1500011106043928 1105 54-55 -0.02738398285116972 1105 56-57 -0.11303010105793021 1105 58-59 -0.22127447439429915 1105 60-61 -0.15473726784968278 1105 62-63 -0.05904831577594649 1105 64-65 0.10771981030345756 1105 66-67 0.25800845712872444 1105 68-69 0.0852521965294386 1105 70-71 0.24803375446516185 1105 72-73 0.26608471111154586 1105 74-75 0.3208641306662585 1105 76-77 0.3015473138285891 1105 78-79 0.21319086702024492 1105 80-81 0.18803248376913828 1105 82-83 0.11565787608939715 1105 84-85 0.2039299823195897 1105 86-87 0.1636460022933548 1105 88-89 0.41422649787335075 1105 90-91 0.5239938780599118 1105 92-93 0.5575475975768995 1105 94-95 0.7479568416932949 1105 96-97 0.6347902041898266 1105 98-99 0.7839863149354933 1105 100-101 1.4258833350099245 1106 1 -0.06712708981437032 1106 2 -0.007514803499617528 1106 3 0.055623399007060925 1106 4 0.032049580689630375 1106 5 -0.07953455708697987 1106 6 -0.019796672807522953 1106 7 -0.11894535527944328 1106 8 -0.14424978448810322 1106 9 -0.09262024698781346 1106 10-11 -0.2475478492728982 1106 12-13 -0.19674857604533003 1106 14-15 -0.09371600432654503 1106 16-17 -0.012275403726782486 1106 18-19 -0.01008722068382184 1106 20-21 -0.026520744804351182 1106 22-23 -0.16246575148875309 1106 24-25 -0.17196635688902262 1106 26-27 -0.28424907962359924 1106 28-29 -0.19887021804388638 1106 30-31 -0.20319528814211196 1106 32-33 -0.21066662076083986 1106 34-35 -0.1600409026587073 1106 36-37 -0.23740270797432572 1106 38-39 -0.2634286339952254 1106 40-41 -0.18777297198914056 1106 42-43 -0.2246760766781577 1106 44-45 -0.09747429619542203 1106 46-47 -0.3029355486945846 1106 48-49 -0.5349528059978539 1106 50-51 -0.3379553591957176 1106 52-53 -0.3231460456309705 1106 54-55 -0.10435976548554038 1106 56-57 -0.2197779229716943 1106 58-59 -0.21135513741851497 1106 60-61 -0.15480759419345702 1106 62-63 -0.03252826999921865 1106 64-65 -0.12706884149807962 1106 66-67 -0.06264533790966453 1106 68-69 0.04377238997302868 1106 70-71 -0.0484973663263375 1106 72-73 -0.08286482373782889 1106 74-75 -0.09030483714236581 1106 76-77 -0.00876592450572744 1106 78-79 -0.09659372394254362 1106 80-81 -0.287817339030628 1106 82-83 -0.3606534056589794 1106 84-85 -0.3295752598540638 1106 86-87 -0.32300915866352753 1106 88-89 -0.06026552811601604 1106 90-91 0.02228906412015874 1106 92-93 -0.2098394372724819 1106 94-95 -0.05378433521160986 1106 96-97 0.06077366550110597 1106 98-99 0.17602891682562927 1106 100-101 0.897196462594092 1107 1 -0.03543219404490827 1107 2 -0.16291793041561675 1107 3 -0.13534724783536944 1107 4 -0.12170310171379839 1107 5 -0.1102005595394644 1107 6 -0.06576885245191022 1107 7 -0.07910514988397921 1107 8 -0.07584504813004145 1107 9 -0.12549808267144869 1107 10-11 -0.03786264847583709 1107 12-13 -0.05980038475164662 1107 14-15 -0.054789732100914534 1107 16-17 -0.07600260329759578 1107 18-19 -0.04517957200203426 1107 20-21 -0.10559914762470868 1107 22-23 -0.018451534750553833 1107 24-25 -0.06561059355363597 1107 26-27 -0.09337250850159506 1107 28-29 -0.12346364232347184 1107 30-31 -0.06117562995694925 1107 32-33 -0.03652613946102434 1107 34-35 -0.14489739407501645 1107 36-37 -0.014505137465434359 1107 38-39 -0.1048250165826019 1107 40-41 -0.15055347474818603 1107 42-43 -0.19575497765914918 1107 44-45 -0.15497295499370267 1107 46-47 -0.1546586097000997 1107 48-49 -0.21358707944973077 1107 50-51 -0.19348225987015155 1107 52-53 -0.160743761756045 1107 54-55 -0.09734566370750031 1107 56-57 0.03839171988544621 1107 58-59 -0.14029309725910366 1107 60-61 0.10272746865142324 1107 62-63 -0.017281680483584694 1107 64-65 -0.1473024021970346 1107 66-67 -0.14421760952217966 1107 68-69 -0.10496553921151985 1107 70-71 -0.042973722856068264 1107 72-73 0.16468709992863495 1107 74-75 0.19165082656088117 1107 76-77 -0.012779758043432565 1107 78-79 0.06250403050258413 1107 80-81 -0.04724637029978496 1107 82-83 -0.11971839646216864 1107 84-85 -0.005290695169822612 1107 86-87 0.03656721319422829 1107 88-89 0.1847406030918286 1107 90-91 0.30961509109747354 1107 92-93 0.30716163568889243 1107 94-95 0.14033947686687043 1107 96-97 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96-97 0.8527026918887337 2315 98-99 0.8830381297464633 2315 100-101 0.7677043106377717 2316 1 -0.5207370624919676 2316 2 -0.22698201661437167 2316 3 -0.019994770391846828 2316 4 0.05414999052570124 2316 5 0.08556790192940866 2316 6 0.07481739823072786 2316 7 0.0853954917150972 2316 8 0.07746128108566808 2316 9 0.15036472568977644 2316 10-11 0.1491683392516947 2316 12-13 0.214521915757949 2316 14-15 0.14988372244940962 2316 16-17 0.20629529299453253 2316 18-19 0.33143941866043747 2316 20-21 0.2165837633923715 2316 22-23 0.22340685780086744 2316 24-25 0.2741333698869326 2316 26-27 0.467047026933777 2316 28-29 0.5373763666555647 2316 30-31 0.41995532661199064 2316 32-33 0.2630389803320554 2316 34-35 0.16335561373473695 2316 36-37 0.26370897235354107 2316 38-39 0.14762327857307156 2316 40-41 0.03607948702725139 2316 42-43 0.13916442878633006 2316 44-45 0.053821810361959876 2316 46-47 -0.24803288476015695 2316 48-49 -0.7657314945224485 2316 50-51 -0.4860393755891579 2316 52-53 -0.08760984344517908 2316 54-55 -0.13594446493910084 2316 56-57 -0.2372523082175988 2316 58-59 -0.11895247348409299 2316 60-61 0.06129725553331866 2316 62-63 -0.042611944862606776 2316 64-65 -0.23864500270027378 2316 66-67 -0.1202375510244238 2316 68-69 -0.1247197548357164 2316 70-71 -0.08231396468698904 2316 72-73 0.10196953418566324 2316 74-75 0.23940656996145293 2316 76-77 0.14834302358170603 2316 78-79 -0.0014793113742364028 2316 80-81 0.04661572107866263 2316 82-83 0.19259796046124222 2316 84-85 0.18022323741369561 2316 86-87 -0.17988586631379988 2316 88-89 0.021510428168134865 2316 90-91 0.27036966521305317 2316 92-93 0.35299526218107147 2316 94-95 0.2872727003075184 2316 96-97 0.6908829551185924 2316 98-99 0.466278233765518 2316 100-101 0.452172555490268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 2.0 4 8.0 5 33.0 6 42.0 7 125.0 8 344.0 9 641.0 10 1154.0 11 1821.0 12 2718.0 13 3930.0 14 4961.0 15 7136.0 16 9610.0 17 13475.0 18 12267.0 19 7838.0 20 6912.0 21 7156.0 22 7508.0 23 8552.0 24 9770.0 25 11916.0 26 14474.0 27 18081.0 28 22724.0 29 28406.0 30 35888.0 31 45089.0 32 57212.0 33 75327.0 34 102932.0 35 145800.0 36 223270.0 37 336249.0 38 265971.0 39 23300.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69228072078891 20.1392463180569 15.297711115492147 21.87076184566205 2 15.711499685981556 21.12954087197964 39.54549961987241 23.613459822166398 3 19.67890787690477 25.061448451393247 29.819720358311574 25.439923313390405 4 13.032624863649886 17.080553994645157 37.215350543747725 32.671470597957224 5 13.52602998012696 38.21507574365233 33.90495318662807 14.353941089592636 6 31.855088751528776 36.963540805870494 17.103031104353285 14.078339338247448 7 28.694939344858362 33.11493075066935 20.23911678180676 17.951013122665522 8 26.78636829405348 35.39067199947113 19.12973919941824 18.693220507057152 9 26.362608666909065 17.1224671933362 20.180808514858033 36.334115624896704 10-11 24.925387392229446 27.43430140485566 26.909757170627767 20.73055403228712 12-13 25.992007544867068 24.491364426269122 27.84510147257796 21.67152655628585 14-15 22.751760156017586 25.735034542028888 26.367335470862397 25.145869831091133 16-17 21.906653885565067 29.395828512874754 26.794598882755427 21.902918718804745 18-19 21.812878061679832 27.72316795028592 29.407926486629428 21.05602750140482 20-21 22.60152712127723 26.52969953393052 29.697980365583575 21.170792979208674 22-23 22.331589364833185 26.52478246670395 29.569256968932507 21.574371199530358 24-25 22.218189522981262 26.752001226998328 29.60235932738543 21.427449922634977 26-27 22.061451380133978 26.895176191606346 29.585698674439374 21.4576737538203 28-29 21.792185829668036 27.114572087602166 29.662550384732096 21.430691697997702 30-31 21.972659122919577 26.7949123504646 29.625288443707987 21.607140082907836 32-33 21.803986381515884 26.93587412818563 29.549928932667836 21.71021055763065 34-35 22.260734472614285 26.92681717515618 29.426602320431034 21.3858460317985 36-37 22.226695609260442 26.622955284750365 29.495927509975044 21.654421596014146 38-39 22.04864410852685 26.974933551583174 29.51096411273142 21.465458227158553 40-41 22.228980059458685 26.977444175673504 29.374202970025394 21.419372794842417 42-43 22.025164135486985 26.968402679061406 29.160644508651412 21.8457886768002 44-45 22.158838116229365 27.00187155364074 29.0737693592755 21.765520970854404 46-47 22.340197666347137 27.325554490463755 28.581859650282617 21.752388192906487 48-49 22.394175784219748 27.448947211846757 28.299871087164906 21.857005916768586 50-51 22.30724225696625 27.59035467556937 28.292566026509768 21.809837040954616 52-53 22.625458632201763 27.749975209070175 27.934717218127126 21.689848940600932 54-55 22.380259808944565 27.52738571381351 28.08967705682081 22.002677420421115 56-57 22.34889101907249 27.83545433510726 27.987240934783326 21.828413711036923 58-59 22.6313754007867 27.49991736356725 28.021981291111626 21.846725944534427 60-61 22.421677189039105 27.52120450864377 28.460086602981523 21.597031699335602 62-63 22.335437807820714 27.512874756222523 28.483753677321253 21.667933758635506 64-65 22.225068588239182 27.640663735827854 28.25666876012296 21.87759891581 66-67 22.213301160215515 27.692129706144847 28.02320431031633 22.071364823323307 68-69 22.18021353254223 27.668958450401615 28.118170098833172 22.032657918222988 70-71 22.26833702442733 27.630912636763295 28.043466763626746 22.057283575182627 72-73 22.360823719961655 27.455326744455093 28.03831025022312 22.14553928536013 74-75 22.249462863187123 28.040491852047733 27.69292301589925 22.017122268865897 76-77 22.3686907083595 27.613393713020194 27.891052127061776 22.126863451558524 78-79 22.380094536079067 27.624731431593563 27.83981753875649 22.155356493570885 80-81 22.34930244888272 27.84793431919765 27.733036547143453 22.069726684776178 82-83 22.41078455645064 27.7413166147102 27.81932569086788 22.02857313797128 84-85 22.319649223131044 27.748526171914385 27.679474826338673 22.252349778615898 86-87 22.23320001322183 27.669883978448418 27.858658645423596 22.238257362906158 88-89 22.327273328264965 27.6487290516643 27.69821174759528 22.325785872475457 90-91 22.23498952757738 27.598767374701165 27.856280544565976 22.30996255315548 92-93 22.31302896839511 27.498720757575956 27.874923807395675 22.31332646663326 94-95 22.197534128846726 27.432188543284962 27.898952170032725 22.471325157835587 96-97 22.18960103130268 27.358047135821238 27.910653488910192 22.541698343965887 98-99 22.070865131874697 27.453710314627067 27.629296439092244 22.846128114405996 100-101 21.88756188380654 27.46037717386705 28.003315015021368 22.64874592730504 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 164.0 1 118.0 2 110.5 3 185.0 4 179.5 5 160.5 6 320.0 7 780.5 8 1336.5 9 1665.5 10 1647.5 11 1551.0 12 1625.5 13 1761.5 14 1913.0 15 2065.5 16 2167.5 17 2255.5 18 2386.0 19 2520.0 20 2724.5 21 3135.0 22 3591.0 23 4166.5 24 4957.0 25 6080.5 26 7918.0 27 9992.0 28 11767.0 29 14140.5 30 17429.5 31 20503.5 32 23801.0 33 27407.5 34 31366.5 35 34771.0 36 38591.0 37 42836.5 38 45660.0 39 47294.5 40 48013.5 41 49725.5 42 51533.0 43 53348.5 44 55841.0 45 57507.5 46 57740.0 47 60107.5 48 64940.5 49 64016.0 50 58787.0 51 54684.5 52 51176.5 53 48975.0 54 47075.0 55 43315.0 56 38434.5 57 34020.5 58 30439.0 59 26919.5 60 22704.5 61 18200.5 62 14064.0 63 10490.0 64 7729.5 65 5835.0 66 4192.0 67 3069.0 68 2201.0 69 1429.5 70 962.5 71 631.0 72 464.0 73 368.0 74 267.0 75 180.0 76 118.5 77 63.5 78 35.5 79 28.0 80 18.0 81 8.5 82 7.0 83 5.5 84 3.5 85 3.0 86 3.0 87 2.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07166231448120848 2 0.0 3 0.0 4 0.0 5 0.0031732390176180876 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.313820117013189E-4 12-13 0.006511750900737117 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.288731696030146E-4 24-25 0.0010246917661058408 26-27 4.627640234026378E-4 28-29 0.0025782567018146963 30-31 4.2970945030244937E-4 32-33 0.0 34-35 0.0 36-37 3.305457310018841E-5 38-39 6.941460351039566E-4 40-41 1.9832743860113047E-4 42-43 0.011899646316067828 44-45 1.9832743860113047E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.610914620037682E-5 82-83 3.636003041020725E-4 84-85 4.958185965028262E-4 86-87 0.0 88-89 0.0 90-91 0.007007569497239944 92-93 0.0023799292632135658 94-95 0.0 96-97 0.0 98-99 1.3221829240075365E-4 100-101 0.009123062175652001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1512650.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.53017867634165 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4265176022893 45.118915706229565 2 12.288563148831084 14.139264672555196 3 3.8693535019300898 6.678137949838988 4 1.6903236160085984 3.889784785992583 5 0.9298839535404921 2.674819499772375 6 0.5829024372393921 2.012068881915435 7 0.3891774366674882 1.5672613227796863 8 0.28182746869393865 1.2970867703890698 9 0.2040233911202781 1.056375193077246 >10 1.2131342084970071 12.832273804011571 >50 0.08382149083131818 3.256366524805896 >100 0.03897711116387958 3.8098372282347768 >500 9.197742690711748E-4 0.3802588237012053 >1k 3.449153509016905E-4 0.4931657313473972 >5k 2.299435672677937E-4 0.7943831053490129 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6807 0.4500049581859651 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5203 0.3439658876805606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3750 0.24790929825141308 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2418 0.15985191551251116 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 6.610914620037682E-5 16-17 3.305457310018841E-5 0.0 0.0 1.3221829240075365E-4 6.610914620037682E-5 18-19 1.6527286550094207E-4 0.0 0.0 1.3221829240075365E-4 6.610914620037682E-5 20-21 2.644365848015073E-4 0.0 0.0 1.6527286550094207E-4 6.610914620037682E-5 22-23 2.644365848015073E-4 0.0 0.0 2.3138201170131887E-4 6.610914620037682E-5 24-25 2.644365848015073E-4 0.0 0.0 2.644365848015073E-4 1.3221829240075365E-4 26-27 2.644365848015073E-4 0.0 0.0 3.966548772022609E-4 1.3221829240075365E-4 28-29 3.305457310018841E-4 0.0 0.0 8.594189006048986E-4 1.3221829240075365E-4 30-31 3.9665487720226094E-4 0.0 0.0 0.0020824381053118697 1.3221829240075365E-4 32-33 3.9665487720226094E-4 0.0 0.0 0.004297094503024493 1.3221829240075365E-4 34-35 4.6276402340263774E-4 0.0 0.0 0.006908405777939377 1.3221829240075365E-4 36-37 4.6276402340263774E-4 0.0 0.0 0.01014775394175784 1.3221829240075365E-4 38-39 4.6276402340263774E-4 0.0 0.0 0.013982084421379697 1.3221829240075365E-4 40-41 4.6276402340263774E-4 0.0 0.0 0.018973324959508146 1.3221829240075365E-4 42-43 4.6276402340263774E-4 0.0 0.0 0.024790929825141306 1.3221829240075365E-4 44-45 5.288731696030146E-4 0.0 0.0 0.03259180907678577 1.3221829240075365E-4 46-47 5.288731696030146E-4 0.0 0.0 0.041847089544838525 1.6527286550094207E-4 48-49 5.288731696030146E-4 0.0 0.0 0.04928436849238092 2.644365848015073E-4 50-51 5.288731696030146E-4 0.0 0.0 0.06148150596635044 2.644365848015073E-4 52-53 5.288731696030146E-4 0.0 0.0 0.09440386077413811 2.974911579016957E-4 54-55 5.288731696030146E-4 0.0 0.0 0.14137440914950583 3.305457310018841E-4 56-57 5.288731696030146E-4 0.0 0.0 0.1768750206591082 3.305457310018841E-4 58-59 5.288731696030146E-4 0.0 0.0 0.2279443360988993 3.305457310018841E-4 60-61 5.288731696030146E-4 0.0 0.0 0.2866823124979341 3.305457310018841E-4 62-63 5.288731696030146E-4 0.0 0.0 0.3284632928965722 3.305457310018841E-4 64-65 5.288731696030146E-4 0.0 0.0 0.3642283409909761 3.305457310018841E-4 66-67 5.288731696030146E-4 0.0 0.0 0.4574752917066076 3.305457310018841E-4 68-69 5.288731696030146E-4 0.0 0.0 0.5514163884573431 3.305457310018841E-4 70-71 5.288731696030146E-4 0.0 0.0 0.6039070505404422 3.305457310018841E-4 72-73 5.288731696030146E-4 0.0 0.0 0.705615971969722 3.305457310018841E-4 74-75 5.61927742703203E-4 0.0 0.0 0.8504611112947476 3.305457310018841E-4 76-77 5.949823158033914E-4 0.0 0.0 0.9928602122103594 3.305457310018841E-4 78-79 5.949823158033914E-4 0.0 0.0 1.1449112484712258 3.305457310018841E-4 80-81 5.949823158033914E-4 0.0 0.0 1.346643308101676 3.305457310018841E-4 82-83 5.949823158033914E-4 0.0 0.0 1.5618285789839024 3.305457310018841E-4 84-85 5.949823158033914E-4 0.0 0.0 1.7642547846494563 3.305457310018841E-4 86-87 5.949823158033914E-4 0.0 0.0 2.0510362608666908 3.636003041020725E-4 88-89 5.949823158033914E-4 0.0 0.0 2.3977126235414667 3.9665487720226094E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9515 0.0 30.274841 1 GGACCGA 305 0.0 28.03148 6 GGTATCA 6505 0.0 25.284061 1 ATCAACG 11480 0.0 24.908197 3 TCAACGC 11515 0.0 24.87374 4 TATCAAC 11545 0.0 24.809105 2 CAACGCA 11565 0.0 24.7662 5 AACGCAG 11850 0.0 24.20984 6 GTACATG 7075 0.0 23.851732 1 AGGACCG 425 0.0 23.47027 5 TACATGG 7165 0.0 23.269049 2 ACATGGG 7065 0.0 22.858854 3 CATGGGG 2915 0.0 22.48683 4 AGTACTT 6080 0.0 22.305122 12-13 ACGCAGA 13330 0.0 21.416395 7 GAGTACT 5920 0.0 21.34332 12-13 CGCAGAG 13425 0.0 21.194082 8 TAACGCA 115 0.003724108 20.651894 4 GTACTTT 6185 0.0 20.312311 14-15 AGAGTAC 10435 0.0 20.279505 10-11 >>END_MODULE