FastQCFastQC Report
Thu 26 May 2016
SRR937331_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937331_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345616
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53961.56127031155965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43251.2513888245914542No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31830.9209643072079996No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14940.4322716540900884No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13290.38453080875885376No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11830.3422873941021249No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT10550.3052520716633489No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.258668580158326No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.2485417341789732No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG7340.21237442710985602No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6140.17765381232350355No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA5810.1681056432572566No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT5660.16376556640896256No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5610.16231887412619786No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5490.15884681264756262No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.13801444377575112No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.13107032081848063No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.11342067496875145No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG3640.1053191981852692No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG3540.10242581361973983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG1351.1881639E-831.6772612
CGGGTCG600.003943490331.6726656
GAGATCC953.4827768E-530.001339
GTACATG31300.028.6958681
AGGCTTA2053.092282E-1127.8101436
CCCTATA1205.3534004E-627.7176042
GCGTTGC350.00839340627.14012186-87
GTATCAA54350.027.0184751
TACATGG32250.026.9625072
ACATGGG31950.026.4720763
GTGTAGC908.909799E-426.4015481
GAGTACT30600.025.84298512-13
CTTACTA1858.916686E-925.6768119
CATGGGA23800.025.5547664
AGTACTT31500.024.19994212-13
TGGGATG5350.023.9765036
CATGGGG6550.023.9393024
ATGGGAT8750.023.8937055
GTACTTT33150.023.78342414-15
GGGTATG1000.001646701223.7613931
Produced by FastQC (version 0.11.5)