##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937331_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 345616 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00217582519328 33.0 31.0 34.0 31.0 34.0 2 32.10493148465349 33.0 31.0 34.0 31.0 34.0 3 32.094234641914724 34.0 31.0 34.0 30.0 34.0 4 35.73644738669506 37.0 35.0 37.0 35.0 37.0 5 35.75359937039952 37.0 35.0 37.0 35.0 37.0 6 35.84544986343225 37.0 35.0 37.0 35.0 37.0 7 35.796077149206056 37.0 35.0 37.0 35.0 37.0 8 35.80004687282996 37.0 35.0 37.0 35.0 37.0 9 37.41850203694273 39.0 37.0 39.0 35.0 39.0 10-11 37.32591951761493 39.0 37.0 39.0 35.0 39.0 12-13 37.195948682931345 39.0 37.0 39.0 34.0 39.0 14-15 38.24268263043378 40.0 38.0 41.0 33.5 41.0 16-17 38.2079301884172 40.0 38.0 41.0 33.5 41.0 18-19 38.253064094254896 40.0 38.0 41.0 34.0 41.0 20-21 38.2175998796352 40.0 38.0 41.0 34.0 41.0 22-23 38.262052393407714 40.0 38.0 41.0 34.0 41.0 24-25 38.2241302485996 40.0 38.0 41.0 34.0 41.0 26-27 38.094555807601495 40.0 38.0 41.0 34.0 41.0 28-29 37.93150924725707 40.0 37.5 41.0 34.0 41.0 30-31 37.5840889889357 39.5 37.0 41.0 33.0 41.0 32-33 37.23672225822878 39.0 35.5 41.0 33.0 41.0 34-35 37.140523008194066 39.0 35.0 41.0 33.0 41.0 36-37 36.96417700569418 39.0 35.0 41.0 32.5 41.0 38-39 36.765864427572794 39.0 35.0 41.0 32.0 41.0 40-41 36.457909934725244 39.0 35.0 41.0 31.0 41.0 42-43 36.10588340817554 39.0 35.0 40.5 30.0 41.0 44-45 35.69375115735383 39.0 35.0 40.0 29.5 41.0 46-47 35.05604630572658 38.0 35.0 40.0 25.5 41.0 48-49 34.53978837785287 38.0 35.0 40.0 23.5 41.0 50-51 33.53525299754641 37.5 33.5 39.5 21.0 40.5 52-53 33.59209932410536 38.0 33.5 39.5 20.0 40.5 54-55 33.85703497523263 38.0 34.0 40.0 18.5 41.0 56-57 33.600555240498124 38.0 34.0 40.0 14.5 41.0 58-59 33.329555055321514 38.0 33.5 40.0 10.0 41.0 60-61 32.9796537197352 37.0 33.0 40.0 7.5 41.0 62-63 32.56378755613166 37.0 33.0 40.0 2.0 41.0 64-65 32.10870590481922 36.0 32.0 39.0 2.0 41.0 66-67 31.653777602888756 35.5 31.5 39.0 2.0 40.5 68-69 31.145197270959677 35.0 31.0 39.0 2.0 40.0 70-71 30.640159888431093 35.0 30.5 38.0 2.0 40.0 72-73 30.10147823017453 35.0 29.5 37.5 2.0 39.5 74-75 29.52978450071756 34.5 29.0 37.0 2.0 39.0 76-77 28.967879091245777 34.0 28.5 36.0 2.0 39.0 78-79 28.41611933475302 34.0 27.5 36.0 2.0 38.5 80-81 27.82838178788019 34.0 26.0 35.0 2.0 37.0 82-83 27.25039205360863 33.5 26.0 35.0 2.0 37.0 84-85 26.66519345169205 33.0 24.5 35.0 2.0 36.0 86-87 26.049874716448315 33.0 23.0 35.0 2.0 36.0 88-89 25.437839972686447 32.0 20.0 35.0 2.0 35.5 90-91 24.829406046016388 32.0 16.5 35.0 2.0 35.0 92-93 24.142040588398686 31.0 4.5 34.0 2.0 35.0 94-95 23.360126556640896 31.0 2.0 34.0 2.0 35.0 96-97 22.473453196611267 31.0 2.0 34.0 2.0 35.0 98-99 21.321657562149902 30.0 2.0 34.0 2.0 35.0 100-101 18.39381133975279 24.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.10419375972077916 1101 2 0.07185334554213085 1101 3 -0.02940963283839082 1101 4 -0.2772360845742412 1101 5 -0.059075791735317296 1101 6 -0.2897904852750628 1101 7 -0.0898220517124031 1101 8 0.029864315412645226 1101 9 -0.07363767430099699 1101 10-11 0.06513882006606764 1101 12-13 0.07839707489286951 1101 14-15 0.19204456580481377 1101 16-17 0.2259489622099693 1101 18-19 0.10001674362856505 1101 20-21 0.1394208679987443 1101 22-23 0.13270174889795783 1101 24-25 0.2750094179099989 1101 26-27 0.2746263079746427 1101 28-29 0.2216556058213328 1101 30-31 0.2636137534059415 1101 32-33 0.4753849886597479 1101 34-35 0.4372200749940447 1101 36-37 0.17442066727147676 1101 38-39 0.02306513152064582 1101 40-41 0.05892602839896455 1101 42-43 0.3066438795969759 1101 44-45 0.07340019485269522 1101 46-47 -0.039260519652764 1101 48-49 0.25125691308048914 1101 50-51 0.38318265494856973 1101 52-53 0.39429939482393905 1101 54-55 0.5638093833705966 1101 56-57 0.5743281082226375 1101 58-59 0.769293068799243 1101 60-61 0.7099768361001892 1101 62-63 0.7135273826691702 1101 64-65 0.6577119814277204 1101 66-67 1.1761521355005264 1101 68-69 0.8281220836782062 1101 70-71 0.8668468485533936 1101 72-73 0.7508740318828409 1101 74-75 1.0836028777258555 1101 76-77 1.2096545998319677 1101 78-79 1.3375581363003697 1101 80-81 1.4673865018315517 1101 82-83 1.3094282849586243 1101 84-85 1.2945328425297298 1101 86-87 1.3414472489528357 1101 88-89 1.6298604986119187 1101 90-91 1.7965444558323753 1101 92-93 2.018711160692856 1101 94-95 2.404988839594406 1101 96-97 2.2061196528639435 1101 98-99 2.4387687825516373 1101 100-101 2.339330800950062 1102 1 0.08669035721081642 1102 2 0.2544331383534342 1102 3 -0.07995906034332023 1102 4 0.1436421606130196 1102 5 0.11511579489388168 1102 6 0.1326290927981617 1102 7 -0.3112182686314142 1102 8 -0.134848598230775 1102 9 -0.09064476233522356 1102 10-11 -0.20219855358657668 1102 12-13 0.0227098711014051 1102 14-15 -0.13019885993858793 1102 16-17 -0.042505091390673044 1102 18-19 0.24793751702117106 1102 20-21 0.1635684165454805 1102 22-23 -0.02373829639835634 1102 24-25 -0.018189843927864047 1102 26-27 -0.13819925927780474 1102 28-29 0.18164910496154363 1102 30-31 0.42576302153495504 1102 32-33 0.5000672853506032 1102 34-35 0.6237213541954887 1102 36-37 0.4636722295748754 1102 38-39 0.46178928536034647 1102 40-41 0.4375591702015882 1102 42-43 0.37189363843604895 1102 44-45 0.3725026567912053 1102 46-47 0.3653865465721253 1102 48-49 0.4635728968073636 1102 50-51 0.07966065687784862 1102 52-53 0.2023615094907214 1102 54-55 0.3364313269179604 1102 56-57 0.3464887004299939 1102 58-59 0.5706918021801073 1102 60-61 0.3872615737590479 1102 62-63 0.4250569720664714 1102 64-65 0.4708384755769437 1102 66-67 0.6687233304004927 1102 68-69 0.8502669479989713 1102 70-71 1.0638700838845203 1102 72-73 1.2199844626172265 1102 74-75 1.0857418703022894 1102 76-77 0.9067145335651396 1102 78-79 0.822684798213718 1102 80-81 0.8521063357450736 1102 82-83 1.044062139758772 1102 84-85 1.0428552012933103 1102 86-87 1.0279432855254136 1102 88-89 0.9541485496122135 1102 90-91 0.9262575791809446 1102 92-93 0.9071689889862746 1102 94-95 0.39730314568004843 1102 96-97 1.060631459264144 1102 98-99 0.51378031633514 1102 100-101 0.6382497708783426 1103 1 0.02759797261041541 1103 2 -0.1918695299579305 1103 3 0.12137834146152215 1103 4 -0.029096916190198385 1103 5 -0.23585479525089426 1103 6 -0.20716834925858052 1103 7 -0.060903394905515995 1103 8 0.01075290796931938 1103 9 0.0071478754626070895 1103 10-11 -0.07203235219724036 1103 12-13 -0.2579042949701389 1103 14-15 -0.25086366549594885 1103 16-17 -0.022226574218684902 1103 18-19 0.029538503841919805 1103 20-21 -0.2835879815587745 1103 22-23 -0.1966169563996587 1103 24-25 -0.19517484684936903 1103 26-27 -0.05459541587067207 1103 28-29 -0.3674127661025395 1103 30-31 -0.25724028950834565 1103 32-33 -0.18802269712034558 1103 34-35 -0.02253262230259878 1103 36-37 0.2501359054821748 1103 38-39 0.21583395198373267 1103 40-41 0.3992581898730876 1103 42-43 0.006377180603166721 1103 44-45 -0.12963653687236132 1103 46-47 -0.07376167130750133 1103 48-49 -0.0426271940840266 1103 50-51 0.3607162953898353 1103 52-53 0.07048135627380958 1103 54-55 0.4766442724480555 1103 56-57 0.44821888286198686 1103 58-59 0.8229965480088453 1103 60-61 0.36959618389992244 1103 62-63 0.6279654963799146 1103 64-65 0.446901580166962 1103 66-67 0.888641528154178 1103 68-69 0.8364806725980749 1103 70-71 1.0378524249869017 1103 72-73 0.8860722377259762 1103 74-75 0.9315965739314542 1103 76-77 0.5276325365515717 1103 78-79 0.2983745989018942 1103 80-81 0.5947019340051511 1103 82-83 0.9769485904501956 1103 84-85 0.9498668224060793 1103 86-87 1.1267454669186492 1103 88-89 1.4741446542671497 1103 90-91 2.066662045843284 1103 92-93 1.474724010735283 1103 94-95 1.5783977376426535 1103 96-97 1.0955759505970022 1103 98-99 0.9797252621249193 1103 100-101 1.7082815828630231 1104 1 0.21424909473224218 1104 2 0.29594014652122524 1104 3 0.06409912257490191 1104 4 0.347622699082315 1104 5 0.2945299322338073 1104 6 0.24796135053740187 1104 7 0.3879599102533291 1104 8 0.12040752761373597 1104 9 0.2531840165293531 1104 10-11 0.4184150392338353 1104 12-13 0.39754601106307774 1104 14-15 0.6506446411201949 1104 16-17 0.7257842098092908 1104 18-19 0.5272432546434302 1104 20-21 0.46002903971781706 1104 22-23 0.7034158328746116 1104 24-25 0.5081222968405186 1104 26-27 0.6325038263327656 1104 28-29 0.44416549096537494 1104 30-31 0.5139197221500638 1104 32-33 0.8169284832935304 1104 34-35 0.9225453433050959 1104 36-37 1.0226366341327164 1104 38-39 1.0863418214095546 1104 40-41 1.021915578389546 1104 42-43 0.9165454988777171 1104 44-45 0.9773730813147594 1104 46-47 1.376150890627791 1104 48-49 1.6036858341876226 1104 50-51 1.4568034419943174 1104 52-53 1.7366901875183558 1104 54-55 2.0851330512297466 1104 56-57 2.189996061527104 1104 58-59 2.5114437937098018 1104 60-61 1.9371821646635539 1104 62-63 1.9719650591895999 1104 64-65 2.2841829850051987 1104 66-67 1.9299414407161173 1104 68-69 2.060234680176041 1104 70-71 1.997248373689903 1104 72-73 2.0505468806872074 1104 74-75 2.369471828959142 1104 76-77 2.3245576312761393 1104 78-79 2.7461558580100274 1104 80-81 2.677469348753039 1104 82-83 2.514674472117349 1104 84-85 2.587191946276672 1104 86-87 2.217094490526925 1104 88-89 2.186382340073038 1104 90-91 2.331930917892251 1104 92-93 2.208482390216485 1104 94-95 1.9321612176776313 1104 96-97 2.5737528669479204 1104 98-99 2.2758051222240177 1104 100-101 1.8846259431078494 1105 1 0.09274693049399829 1105 2 0.06297209189707331 1105 3 0.3340878441502255 1105 4 0.15627812796727625 1105 5 0.016608210597517825 1105 6 0.06788758354355906 1105 7 0.13671013887004335 1105 8 0.10747544201964132 1105 9 0.2694158033976066 1105 10-11 0.3914916105927375 1105 12-13 0.3198010863541825 1105 14-15 0.12398031142441113 1105 16-17 0.23720742507479287 1105 18-19 0.1030754194913257 1105 20-21 0.40810256285382707 1105 22-23 0.47202142331538255 1105 24-25 0.37814226270042184 1105 26-27 0.3973219998376152 1105 28-29 0.32600875134457397 1105 30-31 0.4760760350196591 1105 32-33 0.5884070238044075 1105 34-35 0.6801011257839136 1105 36-37 0.6226640681386826 1105 38-39 0.5298544410523007 1105 40-41 0.7573184010439391 1105 42-43 0.7139644161489471 1105 44-45 0.39386701535121205 1105 46-47 1.0856095262432248 1105 48-49 0.6238955519221818 1105 50-51 0.6214455806274941 1105 52-53 0.6938436045762089 1105 54-55 0.6930630030678273 1105 56-57 0.7956970003797537 1105 58-59 0.9004389470354113 1105 60-61 1.02390791605049 1105 62-63 0.9152795443447559 1105 64-65 0.9279943304907796 1105 66-67 0.5178521887500089 1105 68-69 0.5233511832547428 1105 70-71 0.5793408262900392 1105 72-73 0.26547326610451094 1105 74-75 0.26468916725091773 1105 76-77 0.39001821775422485 1105 78-79 0.5994029774394001 1105 80-81 0.6070935028711872 1105 82-83 0.9191317360324867 1105 84-85 0.8135948908599779 1105 86-87 0.48464082351479476 1105 88-89 0.5975304532230723 1105 90-91 0.9911586506240688 1105 92-93 0.9870213769871974 1105 94-95 1.2502547066735445 1105 96-97 1.3455857633693071 1105 98-99 1.608869195869083 1105 100-101 1.887784664073525 1106 1 -0.0587033972526001 1106 2 -0.15214350256067277 1106 3 -0.14163535716861375 1106 4 -0.05032979290252371 1106 5 -0.0714712336070562 1106 6 -0.07497656843666078 1106 7 0.07471304345065022 1106 8 0.03541044221589118 1106 9 -0.055180891660683073 1106 10-11 -0.17408714671778114 1106 12-13 -0.08084950044959527 1106 14-15 -0.09367188467403054 1106 16-17 3.761655073404313E-4 1106 18-19 0.09803165452685647 1106 20-21 -0.01406743361356888 1106 22-23 0.10612276962773848 1106 24-25 0.13667446821912677 1106 26-27 0.19197992659508145 1106 28-29 0.4435461380070578 1106 30-31 0.31365012145055715 1106 32-33 0.30204579603033466 1106 34-35 -0.03109426613821853 1106 36-37 -0.24335724520276614 1106 38-39 -0.13759070555051522 1106 40-41 -0.048344549852949115 1106 42-43 -0.23951323035574035 1106 44-45 -0.14573242728332048 1106 46-47 0.006032849240440896 1106 48-49 0.08750979221735378 1106 50-51 0.03742862415695214 1106 52-53 -0.1302035752330326 1106 54-55 -0.3394516179629079 1106 56-57 -0.2685619390558216 1106 58-59 -0.9715239999363661 1106 60-61 -0.3687599804836381 1106 62-63 -0.5179249145789839 1106 64-65 0.16539473085189016 1106 66-67 0.22391550075692024 1106 68-69 0.22141217944738756 1106 70-71 0.09093461676772208 1106 72-73 -0.07454420063019285 1106 74-75 -0.05401986442470985 1106 76-77 0.35982431737349074 1106 78-79 0.1367307632854562 1106 80-81 0.25977042715583565 1106 82-83 0.38448283702554065 1106 84-85 0.5567161374745737 1106 86-87 0.6853071107542661 1106 88-89 0.833733695363037 1106 90-91 0.7204291691309557 1106 92-93 0.7000664764887112 1106 94-95 1.261274449971424 1106 96-97 0.9448910190901536 1106 98-99 0.6978759470564277 1106 100-101 0.006911719849323816 1107 1 0.14435995337026597 1107 2 0.0016225124235873523 1107 3 0.11433839820665526 1107 4 -0.03415964616315392 1107 5 0.11166482171945091 1107 6 0.16170162458546145 1107 7 -0.07422076866976113 1107 8 0.009609138851082832 1107 9 0.19116170708915092 1107 10-11 -0.08596594265696211 1107 12-13 0.2086559745692469 1107 14-15 0.28674771899666496 1107 16-17 0.3334324229883876 1107 18-19 0.40842310729096454 1107 20-21 0.25168709934737166 1107 22-23 0.4376606514382644 1107 24-25 -0.029133263305460844 1107 26-27 -0.05049469325685152 1107 28-29 -0.10927560247262136 1107 30-31 0.22009268916789182 1107 32-33 0.2688022352731494 1107 34-35 0.2000147022529717 1107 36-37 0.18312344815185355 1107 38-39 0.0016787008421843552 1107 40-41 -0.07446171968936 1107 42-43 -0.17706033325062975 1107 44-45 -0.035107122499887566 1107 46-47 0.08617981027249044 1107 48-49 0.22325479376897306 1107 50-51 0.41890665758338486 1107 52-53 0.6803097909060867 1107 54-55 0.9915487366941846 1107 56-57 0.8598516354443362 1107 58-59 0.679293927979586 1107 60-61 0.5103462836472374 1107 62-63 0.426360673043483 1107 64-65 0.45872939414931224 1107 66-67 1.1510390100888337 1107 68-69 1.198980118446368 1107 70-71 0.9656132087790716 1107 72-73 1.0379763029828766 1107 74-75 0.7418638967137383 1107 76-77 0.9464160627296998 1107 78-79 1.2270353250622854 1107 80-81 0.98430161481377 1107 82-83 0.9846402161096357 1107 84-85 0.8610119214884016 1107 86-87 0.5180696678318881 1107 88-89 1.1795354420492146 1107 90-91 0.9526575239653745 1107 92-93 0.789517644640501 1107 94-95 0.8661753676466439 1107 96-97 1.0037862622519178 1107 98-99 0.977295861052081 1107 100-101 1.1686796854475858 1108 1 0.11070299544146778 1108 2 0.2743975933297378 1108 3 0.06081898073092873 1108 4 0.13574326645820634 1108 5 0.21979588968061137 1108 6 0.10984078316151624 1108 7 0.23281806628169477 1108 8 0.17599263399671372 1108 9 0.22809536404708552 1108 10-11 0.21943454277993624 1108 12-13 0.28256374155953523 1108 14-15 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0.1776387266611792 2316 54-55 0.14431812707216096 2316 56-57 -0.5505554502310162 2316 58-59 -0.6039550152080437 2316 60-61 -0.6527279951654208 2316 62-63 -0.36470016528357974 2316 64-65 -0.3974125051709194 2316 66-67 -0.020226532408184283 2316 68-69 0.04939277851666901 2316 70-71 0.20256370505506638 2316 72-73 -0.032555277512933856 2316 74-75 0.4898780185142684 2316 76-77 0.0024309897206080677 2316 78-79 -0.603781788330199 2316 80-81 -0.2818989704976964 2316 82-83 -0.24308155964484257 2316 84-85 -0.009220809500586569 2316 86-87 0.54757524996905 2316 88-89 0.47522612506743656 2316 90-91 0.8924076052586365 2316 92-93 0.7879769438151847 2316 94-95 1.0773752561587457 2316 96-97 0.6367046128503908 2316 98-99 1.1600818197705927 2316 100-101 1.6786361741981466 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1412.0 3 177.0 4 177.0 5 195.0 6 216.0 7 226.0 8 328.0 9 427.0 10 566.0 11 765.0 12 1276.0 13 2058.0 14 2246.0 15 3163.0 16 4571.0 17 6595.0 18 5402.0 19 3024.0 20 2972.0 21 3003.0 22 3302.0 23 3566.0 24 4127.0 25 4537.0 26 5146.0 27 5768.0 28 6943.0 29 7974.0 30 9480.0 31 11789.0 32 14644.0 33 19239.0 34 25637.0 35 36982.0 36 47244.0 37 57173.0 38 39409.0 39 3857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.075878902565385 20.08974050135104 16.146034381176552 19.688346214907025 2 14.40678457860409 22.142144233224244 41.41424691873825 22.03682426943342 3 17.541894902980225 29.354574784142606 31.623688608300064 21.479841704577105 4 10.808850409966867 18.20798347366405 39.53554496869396 31.447621147675115 5 11.091994208835878 40.07653853184125 34.28305330134073 14.548413957982145 6 26.306019931088088 39.32155411441233 18.65117115444853 15.721254800051046 7 24.578230089008894 36.8033944610887 21.881860921063698 16.736514528838708 8 25.60653388903874 33.73938112515843 20.901890152470944 19.75219483333188 9 27.547166527429685 16.478116587765708 21.21072356817971 34.7639933166249 10-11 23.013516629994765 28.502263408209767 27.42748898755089 21.056730974244577 12-13 25.722171139839716 26.945489457853167 28.251474218222008 19.080865184085113 14-15 22.858017091488627 27.095795575006925 28.81973598537087 21.226451348133583 16-17 18.573986934738947 30.924356343757026 29.427289139592382 21.074367581911645 18-19 18.419018980413533 28.237334187797497 34.525475162785575 18.818171669003394 20-21 20.05617114073524 27.075243809429907 34.18392351213175 18.6846615377031 22-23 19.523370394214094 26.944536275004495 34.2056269900649 19.32646634071651 24-25 19.161298087031163 27.346043925928825 34.52174329126919 18.970914695770823 26-27 18.901284367487435 27.442951638300773 34.51842744005174 19.137336554160058 28-29 18.460978467678316 27.07825769177224 35.5273156526985 18.933448187850942 30-31 18.393533640039685 28.124836832609112 34.72057040419101 18.761059123160198 32-33 18.131164516171136 28.511263452984707 34.04255936281327 19.315012668030878 34-35 18.47430736031186 27.877297196955634 34.34106413588268 19.30733130684982 36-37 18.86638971659743 27.873501968375493 33.92000348000087 19.34010483502621 38-39 18.81465457872218 27.961804956482528 34.21460499359888 19.00893547119641 40-41 18.244501017739402 28.364049153043652 34.44888396611015 18.942565863106804 42-43 18.208365715876532 28.807462097924812 33.3828254590588 19.601346727139855 44-45 18.286559416528313 29.223028571842853 32.80476753206267 19.685644479566168 46-47 18.399090464817146 29.174298090295146 32.09403364062049 20.332577804267217 48-49 18.763487017657845 29.51031974383368 31.541783650834166 20.184409587674317 50-51 19.43293003071907 29.588642936499422 31.139970470242535 19.83845656253898 52-53 18.840314827225072 29.95950066290104 31.25658186755324 19.943602642320645 54-55 18.505122869096734 29.770214123601452 31.473631544981906 20.2510314623199 56-57 19.017634615635554 30.42409778998236 30.284041564512133 20.27422602986996 58-59 18.838003922802265 29.386514896066036 31.063926020530204 20.711555160601495 60-61 19.1279098423633 29.481280461109804 31.23769595862073 20.15311373790616 62-63 19.54944009835248 29.565039636745322 31.187478615874415 19.698041649027783 64-65 19.383771449778127 29.209824748589824 31.681958339192445 19.7244454624396 66-67 18.883278314914122 29.703857378339706 31.247127661579455 20.165736645166714 68-69 18.744119356938544 30.08961048939998 31.16099297874777 20.0052771749137 70-71 18.829168291653918 29.682101750056905 31.051585431802003 20.437144526487174 72-73 18.706859181699954 29.544891097750337 30.47494226143789 21.27330745911182 74-75 18.659927739211625 29.701590781240032 30.930633122814342 20.707848356733997 76-77 18.781365957070417 28.964055211334593 31.569178746623244 20.685400084971747 78-79 18.48568292318646 28.884432399355596 31.664034824420597 20.965849853037348 80-81 18.277527501765302 29.979425330187926 31.083990760972796 20.659056407073976 82-83 18.42333036122045 29.662846414254545 30.518524962123685 21.395298262401322 84-85 18.29619020836293 28.84247971309871 30.5693668515367 22.291963227001656 86-87 18.659522711919447 29.007239632712583 30.82149680928482 21.511740846083146 88-89 19.161774747985845 27.991150327005855 31.02851351880338 21.81856140620492 90-91 18.42710736037555 27.88473108801454 31.57513979479849 22.113021756811417 92-93 18.398190570086413 28.51910920373485 31.350403062112157 21.732297164066576 94-95 18.00358677041335 28.06988548140867 31.44484216905374 22.481685579124242 96-97 18.095681348284632 28.104293713553314 30.866920107988044 22.933104830174017 98-99 18.123424067417655 28.325969918990356 30.691735624667416 22.858870388924572 100-101 18.605996378571234 27.657973594594242 31.53919421035311 22.196835816481414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 916.0 1 598.0 2 365.0 3 495.5 4 482.0 5 499.0 6 846.0 7 1812.0 8 2868.0 9 3163.5 10 2547.0 11 1817.0 12 1664.5 13 1729.5 14 1742.5 15 1642.5 16 1561.0 17 1570.5 18 1533.0 19 1549.0 20 1707.5 21 1886.5 22 1962.0 23 2067.5 24 2287.0 25 2537.0 26 2957.5 27 3299.5 28 3549.0 29 4134.0 30 5025.0 31 5791.0 32 6847.5 33 8360.0 34 9470.5 35 10162.0 36 11689.5 37 12846.5 38 12632.0 39 12142.5 40 11553.0 41 11183.0 42 10682.0 43 10785.0 44 11280.0 45 11263.0 46 10942.0 47 11130.5 48 14353.0 49 14206.0 50 10392.5 51 8523.5 52 7227.5 53 6660.5 54 6379.0 55 5915.5 56 5326.0 57 4734.5 58 4251.5 59 3873.0 60 3377.5 61 2882.5 62 2313.0 63 1658.5 64 1159.5 65 893.0 66 651.5 67 490.5 68 351.0 69 220.5 70 157.0 71 119.5 72 95.0 73 79.0 74 66.5 75 48.5 76 31.0 77 20.5 78 11.5 79 6.5 80 4.5 81 5.5 82 4.0 83 2.0 84 3.5 85 2.5 86 0.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30872413314198416 2 0.27545021063839636 3 0.2731355029859729 4 0.27602888755150223 5 0.2757395490949493 6 0.23957224202583216 7 0.2369681959168557 8 0.23986158048238507 9 0.25461784176658486 10-11 0.25823457247349657 12-13 0.24796305726586731 14-15 0.23855955742789683 16-17 0.23393014212304986 18-19 0.22004189620850886 20-21 0.22481598074163234 22-23 0.22510531919818524 24-25 0.2272753576223323 26-27 0.22481598074163234 28-29 0.2407295958520439 30-31 0.254039164853479 32-33 0.24608235729827324 34-35 0.2453590111568909 36-37 0.2281433729919911 38-39 0.21830586546919126 40-41 0.21237442710985602 42-43 0.22568399611129114 44-45 0.2266966807092264 46-47 0.23855955742789683 48-49 0.24304430350446737 50-51 0.25432850331003193 52-53 0.2659020415721494 54-55 0.24261029581963797 56-57 0.21830586546919126 58-59 0.20528563492430907 60-61 0.2051409656960326 62-63 0.21237442710985602 64-65 0.2058643118374149 66-67 0.21165108096847365 68-69 0.2125190963381325 70-71 0.21454446553400308 72-73 0.214833803990556 74-75 0.21816119624091476 76-77 0.23016874218786168 78-79 0.23161543447062635 80-81 0.22409263460025 82-83 0.21512314244710892 84-85 0.19935419656497383 86-87 0.2051409656960326 88-89 0.21541248090366188 90-91 0.21280843479468542 92-93 0.21873987315402066 94-95 0.21338711170779132 96-97 0.22076524234989123 98-99 0.2258286653395676 100-101 0.20962571177260308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 345616.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.07470691737035 #Duplication Level Percentage of deduplicated Percentage of total 1 78.64679212454739 40.16861857752608 2 11.519952165384133 11.767563610982409 3 3.829559268361525 5.867808517627923 4 1.7279133068771952 3.5301066290950778 5 0.9613722335072806 2.4550902532441037 6 0.6227935170642013 1.908539781245542 7 0.4222181449913903 1.509526760744173 8 0.30666207571225423 1.2530140511740655 9 0.23164020057285062 1.0647859819085304 >10 1.5503758724948045 14.876067512427351 >50 0.11727042548841211 4.160279587142038 >100 0.05495280807911099 4.690080110445493 >500 0.004532190357040082 1.5503392702675645 >1k 0.0033991427677800613 3.636844771928521 >5k 5.665237946300102E-4 1.561334584241093 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5396 1.56127031155965 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4325 1.2513888245914542 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3183 0.9209643072079996 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1494 0.4322716540900884 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1329 0.38453080875885376 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1183 0.3422873941021249 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1055 0.3052520716633489 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 894 0.258668580158326 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 859 0.2485417341789732 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 734 0.21237442710985602 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 614 0.17765381232350355 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 581 0.1681056432572566 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 566 0.16376556640896256 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 561 0.16231887412619786 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 549 0.15884681264756262 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 477 0.13801444377575112 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 453 0.13107032081848063 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 392 0.11342067496875145 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 364 0.1053191981852692 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 354 0.10242581361973983 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4466922827646868E-4 0.0 12-13 0.0 0.0 0.0 2.8933845655293737E-4 0.0 14-15 0.0 0.0 0.0 2.8933845655293737E-4 0.0 16-17 0.0 0.0 0.0 5.786769131058747E-4 0.0 18-19 0.0 0.0 0.0 5.786769131058747E-4 0.0 20-21 0.0 0.0 0.0 7.233461413823435E-4 0.0 22-23 0.0 0.0 0.0 0.0020253691958705615 0.0 24-25 0.0 0.0 0.0 0.003761399935188186 0.0 26-27 0.0 0.0 0.0 0.004629415304846998 0.0 28-29 0.0 0.0 0.0 0.008101476783482246 0.0 30-31 0.0 0.0 0.0 0.015768945882135087 0.0 32-33 0.0 0.0 0.0 0.02661913800287024 0.0 34-35 0.0 0.0 0.0 0.03732466089532892 0.0 36-37 0.0 0.0 0.0 0.05237026063608166 0.0 38-39 0.0 0.0 0.0 0.07233461413823433 0.0 40-41 0.0 0.0 0.0 0.0956263598907458 0.0 42-43 0.0 0.0 0.0 0.11443335956668672 0.0 44-45 0.0 0.0 0.0 0.1270195824267395 0.0 46-47 0.0 0.0 0.0 0.14727327438544513 0.0 48-49 0.0 0.0 0.0 0.16694828943104487 0.0 50-51 0.0 0.0 0.0 0.18459793528077403 0.0 52-53 0.0 0.0 0.0 0.21931855006712653 0.0 54-55 0.0 0.0 0.0 0.2860110643025786 0.0 56-57 0.0 0.0 0.0 0.3939343085968242 0.0 58-59 0.0 0.0 0.0 0.5346974677098282 0.0 60-61 0.0 0.0 0.0 0.715534003055414 0.0 62-63 0.0 0.0 0.0 0.8189725012730893 0.0 64-65 0.0 0.0 0.0 0.939915976112217 0.0 66-67 0.0 0.0 0.0 1.1793435489097726 0.0 68-69 0.0 0.0 0.0 1.494577797324198 0.0 70-71 0.0 0.0 0.0 1.8678244062774874 0.0 72-73 0.0 0.0 0.0 2.295611314291005 0.0 74-75 0.0 0.0 0.0 2.7099439840748114 0.0 76-77 0.0 0.0 0.0 3.0829012545715475 0.0 78-79 0.0 0.0 0.0 3.4079730105087727 0.0 80-81 0.0 0.0 0.0 3.6579614369705107 0.0 82-83 0.0 0.0 0.0 4.0178984769223645 0.0 84-85 0.0 0.0 0.0 4.696252488310726 0.0 86-87 0.0 0.0 0.0 5.578011434655803 0.0 88-89 0.0 0.0 0.0 6.44501411971668 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATATAGG 135 1.1881639E-8 31.677261 2 CGGGTCG 60 0.0039434903 31.672665 6 GAGATCC 95 3.4827768E-5 30.00133 9 GTACATG 3130 0.0 28.695868 1 AGGCTTA 205 3.092282E-11 27.810143 6 CCCTATA 120 5.3534004E-6 27.717604 2 GCGTTGC 35 0.008393406 27.140121 86-87 GTATCAA 5435 0.0 27.018475 1 TACATGG 3225 0.0 26.962507 2 ACATGGG 3195 0.0 26.472076 3 GTGTAGC 90 8.909799E-4 26.401548 1 GAGTACT 3060 0.0 25.842985 12-13 CTTACTA 185 8.916686E-9 25.676811 9 CATGGGA 2380 0.0 25.554766 4 AGTACTT 3150 0.0 24.199942 12-13 TGGGATG 535 0.0 23.976503 6 CATGGGG 655 0.0 23.939302 4 ATGGGAT 875 0.0 23.893705 5 GTACTTT 3315 0.0 23.783424 14-15 GGGTATG 100 0.0016467012 23.761393 1 >>END_MODULE