FastQCFastQC Report
Thu 26 May 2016
SRR937331_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937331_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345616
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65851.9052937364010925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55081.593676218693579No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40121.1608258876903845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24650.7132192954029906No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15760.45599740752742923No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13240.3830841164760891No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11750.3399726864497014No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10360.29975464098884314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.2659020415721494No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT9020.2609832878107495No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5720.16550159714828017No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT5560.1608721818434332No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.15219202814684504No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.14611592055923336No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC4930.14264385908059812No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA4590.13280635155779824No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG4480.12962362853571593No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG4310.12470487477431601No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4060.11747141336049256No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3890.11255265959909262No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG3750.10850192120735153No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG3710.10734456738113976No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3660.10589787509837507No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA3620.10474052127216332No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT3490.10097912133697515No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT250.00501882756.9985286
CTTACAC652.5845566E-643.845023
CTGGTCG1251.3460522E-1038.0045139
GACGTCG300.003950372331.6658546-47
TATAGGG1252.147026E-730.3992142
AGGGGTC1252.147026E-730.3992145
GTATGGT1102.7174956E-630.2396141
TGTATAC953.484473E-529.9992243
GTATACT804.4878913E-429.6867314
GGTCCTA1153.850728E-628.9164778
GGTACTC1004.9392227E-528.5033878
GTCGGGA700.00837873827.146088
GTGGTAC1056.893314E-527.1421576
GGTATCA47550.026.2830731
ATGGGAG5300.025.0936895
GTATCAA63850.024.7085971
GGTGGTA1351.3394654E-524.6289925
GTACATG29800.024.5570031
TGGGATA1951.5621481E-824.3583436
AGTACTT44200.023.8065612-13
Produced by FastQC (version 0.11.5)