##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937331_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 345616 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.427101175871485 33.0 31.0 34.0 31.0 34.0 2 32.59355469654183 34.0 31.0 34.0 31.0 34.0 3 32.61215915929818 34.0 31.0 34.0 31.0 34.0 4 36.229213925281236 37.0 35.0 37.0 35.0 37.0 5 36.19603259108374 37.0 35.0 37.0 35.0 37.0 6 36.28689643997963 37.0 37.0 37.0 35.0 37.0 7 36.23311710106014 37.0 37.0 37.0 35.0 37.0 8 36.23747453821582 37.0 37.0 37.0 35.0 37.0 9 37.95354960418499 39.0 38.0 39.0 35.0 39.0 10-11 37.88370908754224 39.0 38.0 39.0 35.0 39.0 12-13 37.77333803990556 39.0 37.5 39.0 35.0 39.0 14-15 38.89870549974538 40.0 38.0 41.0 35.5 41.0 16-17 38.88340528216287 40.0 38.0 41.0 35.5 41.0 18-19 39.000920096291836 40.0 38.0 41.0 35.5 41.0 20-21 38.98350336789964 40.0 38.0 41.0 35.0 41.0 22-23 38.906608779686124 40.0 38.0 41.0 35.0 41.0 24-25 38.90991013147539 40.0 38.0 41.0 35.0 41.0 26-27 38.718653071617055 40.0 38.0 41.0 35.0 41.0 28-29 38.534253333179024 40.0 38.0 41.0 35.0 41.0 30-31 38.38865822184158 40.0 37.5 41.0 35.0 41.0 32-33 38.099358825980275 40.0 36.5 41.0 34.0 41.0 34-35 37.89637343178556 40.0 36.0 41.0 34.0 41.0 36-37 37.669513274848384 40.0 35.5 41.0 33.5 41.0 38-39 37.49523170223601 40.0 35.0 41.0 33.0 41.0 40-41 37.225817091801304 40.0 35.0 41.0 33.0 41.0 42-43 36.90437508680154 40.0 35.0 41.0 33.0 41.0 44-45 36.379533933614184 39.0 35.0 41.0 31.5 41.0 46-47 35.66942936669599 39.0 35.0 41.0 29.0 41.0 48-49 35.04580372436462 39.0 35.0 41.0 24.5 41.0 50-51 34.69407087634832 39.0 35.0 40.5 23.0 41.0 52-53 34.40884391926299 39.0 35.0 40.0 20.5 41.0 54-55 34.18851123790565 38.0 35.0 40.0 19.5 41.0 56-57 33.91509218323226 38.0 34.0 40.0 17.0 41.0 58-59 33.64323266283968 38.0 34.0 40.0 13.0 41.0 60-61 33.2852370839313 37.5 34.0 40.0 7.5 41.0 62-63 32.897617587148744 37.0 33.0 40.0 3.5 41.0 64-65 32.45202768390352 36.0 33.0 39.0 2.0 41.0 66-67 31.992752071663347 36.0 32.5 39.0 2.0 40.0 68-69 31.537366614971532 35.0 32.0 39.0 2.0 40.0 70-71 31.069431102726725 35.0 31.0 38.0 2.0 40.0 72-73 30.58075146983936 35.0 31.0 37.0 2.0 39.5 74-75 30.084660432387388 35.0 30.5 37.0 2.0 39.0 76-77 28.786474005833064 33.5 28.5 35.5 2.0 38.0 78-79 29.3130960372205 34.0 30.0 36.0 2.0 38.0 80-81 29.089091650849497 34.0 29.5 35.5 2.0 37.0 82-83 28.6857957964909 34.0 29.0 35.0 2.0 37.0 84-85 28.24807445257164 34.0 29.0 35.0 2.0 36.5 86-87 27.872248391278184 34.0 28.5 35.0 2.0 36.0 88-89 27.417308515809452 34.0 27.0 35.0 2.0 36.0 90-91 26.865195766399705 33.0 25.5 35.0 2.0 35.0 92-93 26.392065182167492 33.0 25.0 35.0 2.0 35.0 94-95 25.933849995370586 33.0 24.0 35.0 2.0 35.0 96-97 25.300450499976854 32.5 19.0 35.0 2.0 35.0 98-99 24.430758413962316 32.0 4.5 34.0 2.0 35.0 100-101 21.739196102032313 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24365925992088222 1101 2 0.2509607179026787 1101 3 0.23706205275678371 1101 4 0.1957921659945896 1101 5 0.09004898371642156 1101 6 0.1875885023631838 1101 7 0.2103246470542075 1101 8 0.06751861963005013 1101 9 0.12223488790044712 1101 10-11 0.2174722272766374 1101 12-13 0.22623942036684497 1101 14-15 0.29610799567071666 1101 16-17 0.1374110785122653 1101 18-19 0.2229461649382145 1101 20-21 0.27049028090265637 1101 22-23 0.1701800486415621 1101 24-25 0.0491212350356065 1101 26-27 0.14320996875571979 1101 28-29 -0.05905198520534327 1101 30-31 0.23073635160264416 1101 32-33 -0.03737251000315922 1101 34-35 -0.02334456715644251 1101 36-37 0.15696979334594374 1101 38-39 0.0584670478017415 1101 40-41 0.057423958623132876 1101 42-43 -0.23285814248413317 1101 44-45 -0.2741051960367713 1101 46-47 0.01073050028296052 1101 48-49 0.00795375208870297 1101 50-51 0.07724278266108797 1101 52-53 -0.05873409955597708 1101 54-55 -0.024568624571557507 1101 56-57 -0.0336778602795178 1101 58-59 -0.2327926360955459 1101 60-61 -0.21888302689367833 1101 62-63 -0.21426630391250967 1101 64-65 -0.10920306497848742 1101 66-67 -0.2865131518130646 1101 68-69 -0.2576804046382648 1101 70-71 -0.4974930251046672 1101 72-73 -0.1068573713731702 1101 74-75 -0.22224857131176634 1101 76-77 -0.29704258660716576 1101 78-79 -0.06021804039848888 1101 80-81 -0.12367504441163035 1101 82-83 -0.3421012747916272 1101 84-85 -0.4550997099136822 1101 86-87 -0.5178533318040088 1101 88-89 0.23495843110632464 1101 90-91 0.34104060367807776 1101 92-93 0.4778288100285373 1101 94-95 0.22773540827869354 1101 96-97 0.31018973239029535 1101 98-99 0.8683459643158429 1101 100-101 1.5893224904568335 1102 1 -0.054174485832582775 1102 2 0.0876737993102239 1102 3 0.04170073077548864 1102 4 0.06153892604994837 1102 5 0.07384926052722562 1102 6 0.15363723784110306 1102 7 -0.007192024505577876 1102 8 -0.006507299927058341 1102 9 -0.05535392223250568 1102 10-11 -0.06478566812243258 1102 12-13 0.12047462566818723 1102 14-15 0.09912670137050128 1102 16-17 0.10533667512342504 1102 18-19 0.1502099320816015 1102 20-21 0.005711310135545489 1102 22-23 0.16075380194441635 1102 24-25 -0.13333273468297335 1102 26-27 -0.22127331774346715 1102 28-29 0.08211628221066292 1102 30-31 0.17044087703987998 1102 32-33 0.12509655848654688 1102 34-35 0.2572042312330183 1102 36-37 0.21304495167333215 1102 38-39 0.22789832742259364 1102 40-41 -0.0010156253218411848 1102 42-43 0.07185393233866932 1102 44-45 0.319816500058522 1102 46-47 0.11372718694151729 1102 48-49 0.18596197253076951 1102 50-51 -0.13659880606516595 1102 52-53 0.06981516018482381 1102 54-55 0.0376980784440093 1102 56-57 0.06935028215222161 1102 58-59 0.10379986484812775 1102 60-61 0.37508585286138896 1102 62-63 0.2674211934661699 1102 64-65 0.5594051219107428 1102 66-67 0.5260681089343286 1102 68-69 0.4715482998032918 1102 70-71 0.6500502790097507 1102 72-73 0.8870244807427561 1102 74-75 0.7003387708851037 1102 76-77 0.7597875102766132 1102 78-79 0.47330332855676005 1102 80-81 0.35763498368886104 1102 82-83 0.3403631801846778 1102 84-85 0.7621338596874558 1102 86-87 0.8481031334059068 1102 88-89 0.9153520476814201 1102 90-91 0.9678597958938262 1102 92-93 1.1011197130189352 1102 94-95 1.2194887530759075 1102 96-97 1.3538398603104405 1102 98-99 1.5774220034048838 1102 100-101 1.9839351650363781 1103 1 0.07108682689869994 1103 2 0.03142501660555297 1103 3 0.14176565319319678 1103 4 -0.08968486152389943 1103 5 -0.009472170362862187 1103 6 -0.17775275112208533 1103 7 -0.10117371638378359 1103 8 -0.15328390188771124 1103 9 0.03995218696699965 1103 10-11 0.010274385113945073 1103 12-13 0.034773788169005115 1103 14-15 -0.18872956039668765 1103 16-17 0.03425311997182945 1103 18-19 0.0648239683083105 1103 20-21 0.03203410439640919 1103 22-23 0.07789007409918725 1103 24-25 0.0658687195791785 1103 26-27 0.11867669199489939 1103 28-29 -0.12518424366091807 1103 30-31 0.0195319631919304 1103 32-33 0.05188679415492459 1103 34-35 0.03346534919705846 1103 36-37 0.03279110502008109 1103 38-39 0.03791910259626263 1103 40-41 0.13655173168652368 1103 42-43 0.3329134661957127 1103 44-45 0.1627204798027293 1103 46-47 0.0692163773589698 1103 48-49 0.41424542380649143 1103 50-51 0.4884774777756462 1103 52-53 0.47090585680236785 1103 54-55 0.7440669676214355 1103 56-57 0.6743655389203838 1103 58-59 0.9304180561095663 1103 60-61 1.0604296170631642 1103 62-63 0.7965259133259366 1103 64-65 0.30899611067916766 1103 66-67 0.5187935520298232 1103 68-69 0.5813061391852301 1103 70-71 0.542132990169911 1103 72-73 0.5120812878582548 1103 74-75 0.48312783794995795 1103 76-77 0.664734598506179 1103 78-79 0.6171064837862588 1103 80-81 1.0239337566551647 1103 82-83 0.4400905060302911 1103 84-85 0.4699699955051564 1103 86-87 0.23915357812446913 1103 88-89 0.29102886388605853 1103 90-91 0.4230990350030872 1103 92-93 0.7120299253152389 1103 94-95 0.5620031983138496 1103 96-97 0.31900898320857607 1103 98-99 0.7262014619519235 1103 100-101 1.1891224662114759 1104 1 0.1317688438033926 1104 2 0.1898846493656805 1104 3 0.25624393940712764 1104 4 0.1353515710030635 1104 5 0.07590381331537088 1104 6 -0.012844561090020079 1104 7 0.18495277734982096 1104 8 0.08665343416591753 1104 9 0.21854443412526336 1104 10-11 0.23692477858524086 1104 12-13 0.13225994380875505 1104 14-15 0.1987157908128765 1104 16-17 0.2336264805081143 1104 18-19 0.15106704612819044 1104 20-21 -0.06878198420749726 1104 22-23 0.09674755005372049 1104 24-25 0.17140282042441157 1104 26-27 0.06291670948251493 1104 28-29 0.11218459924202762 1104 30-31 6.125519415647318E-4 1104 32-33 -0.16319627200654452 1104 34-35 0.037272548264382976 1104 36-37 0.25053738890233035 1104 38-39 0.47203087758897766 1104 40-41 0.3461407727824195 1104 42-43 0.13165654401559124 1104 44-45 0.23274379661998523 1104 46-47 -0.28933069346587104 1104 48-49 -0.5574647190090118 1104 50-51 -0.664896174220857 1104 52-53 -0.7136248922503938 1104 54-55 -0.5528471973450451 1104 56-57 -0.899075148925732 1104 58-59 -0.9215165110720065 1104 60-61 -0.498867963817041 1104 62-63 -0.6799860365429033 1104 64-65 -0.6571107094898814 1104 66-67 -0.4069824607944206 1104 68-69 -0.5094691449884792 1104 70-71 -0.7210738008531123 1104 72-73 -0.26999755213008925 1104 74-75 -0.40751264587400016 1104 76-77 -0.3513285026305155 1104 78-79 -0.20444699916331643 1104 80-81 -0.16307440423087272 1104 82-83 -0.21577148755758557 1104 84-85 -0.011915752090299492 1104 86-87 0.28622075970418237 1104 88-89 0.4241059551131059 1104 90-91 0.30357120055249 1104 92-93 0.8584303916515843 1104 94-95 0.8996780744432549 1104 96-97 0.7607169400724629 1104 98-99 0.7229990428321322 1104 100-101 1.2418949815131342 1105 1 0.023922718094681272 1105 2 0.2014450346610559 1105 3 0.12334904213224007 1105 4 0.045032726889182584 1105 5 0.07293941655867542 1105 6 0.13871308080474876 1105 7 0.06518810015662524 1105 8 0.010440241457267518 1105 9 0.10522674833607937 1105 10-11 0.1470212548395864 1105 12-13 0.18625159167805094 1105 14-15 0.015784314863353188 1105 16-17 0.1520103127339283 1105 18-19 0.18755427407429437 1105 20-21 0.21424551076293596 1105 22-23 0.1849631203762243 1105 24-25 -0.003652809643256205 1105 26-27 -0.2522657266572139 1105 28-29 -0.034408249627077225 1105 30-31 0.00945620858973939 1105 32-33 -0.17305727304294294 1105 34-35 -0.2905523922952682 1105 36-37 -0.16243203742212842 1105 38-39 -0.13207736480570986 1105 40-41 -0.2555784249253179 1105 42-43 -0.3306026463764127 1105 44-45 -0.5456046762203499 1105 46-47 -0.6867046494495241 1105 48-49 -0.5723895803148693 1105 50-51 -1.063047427040459 1105 52-53 -0.7719602577323172 1105 54-55 -0.7670724914985527 1105 56-57 -0.7655096426159034 1105 58-59 -0.7842893270203106 1105 60-61 -0.8797281723154882 1105 62-63 -0.6045691920632379 1105 64-65 -0.4779018757399598 1105 66-67 -0.5377397917704627 1105 68-69 -0.37060735141413303 1105 70-71 -0.9391246147286232 1105 72-73 -0.7623427024074765 1105 74-75 -0.6430526385582631 1105 76-77 -1.1117343735077938 1105 78-79 -0.9006153829955394 1105 80-81 -0.8132527252697095 1105 82-83 -0.7031327934162697 1105 84-85 -0.7188748449391689 1105 86-87 -1.0099102833976907 1105 88-89 -0.5735431029860258 1105 90-91 -0.4116028529743012 1105 92-93 -0.8931394431347393 1105 94-95 -0.6401202856128307 1105 96-97 -0.45406084447914097 1105 98-99 -0.12447169422816273 1105 100-101 0.3507584516100657 1106 1 0.18204573100828725 1106 2 0.194438715235691 1106 3 0.3465601737411461 1106 4 0.21921924806514426 1106 5 0.18093878854124767 1106 6 0.18389108449435554 1106 7 0.12964820142443045 1106 8 0.004935276194480309 1106 9 0.03652752943275317 1106 10-11 -0.039725614886052085 1106 12-13 -0.17515771868031038 1106 14-15 0.11058550809646306 1106 16-17 0.2743216131225168 1106 18-19 0.3021527354315978 1106 20-21 0.33066424138271344 1106 22-23 0.15460240286630977 1106 24-25 0.3271700894421983 1106 26-27 0.2762109385702445 1106 28-29 0.3087198659281256 1106 30-31 0.3236415522330205 1106 32-33 0.3604484379905344 1106 34-35 -0.029890815186504938 1106 36-37 0.4156678173488473 1106 38-39 0.4968232121853049 1106 40-41 0.5070996768920111 1106 42-43 0.5421600415381747 1106 44-45 0.6935423976109476 1106 46-47 0.5346273362630711 1106 48-49 0.3919851498338929 1106 50-51 0.3062856969537293 1106 52-53 0.5582346239256566 1106 54-55 0.7259162826899299 1106 56-57 0.4048449909751781 1106 58-59 0.3358975081643649 1106 60-61 0.2867994800768656 1106 62-63 0.3437861872985408 1106 64-65 0.4637906312271056 1106 66-67 0.6753004013448951 1106 68-69 0.8607581939797484 1106 70-71 0.9736398394849815 1106 72-73 0.9196155344335963 1106 74-75 0.5166894817855763 1106 76-77 0.5565154204239882 1106 78-79 0.379092785156125 1106 80-81 0.035920058025030954 1106 82-83 0.4644055745234432 1106 84-85 0.3151754749572113 1106 86-87 0.6127837151107691 1106 88-89 0.632809685803867 1106 90-91 0.5559757473318534 1106 92-93 0.529153212986472 1106 94-95 0.21097580105357494 1106 96-97 0.4200363804688507 1106 98-99 0.6333932427738418 1106 100-101 1.5555608223758561 1107 1 0.17590573012164867 1107 2 0.09187631775375849 1107 3 0.0010022538049838658 1107 4 0.08849486095692782 1107 5 0.16743965301790809 1107 6 0.12989454240099008 1107 7 0.2134248561214207 1107 8 0.1832502116912238 1107 9 0.2931330177512379 1107 10-11 0.1438870109063899 1107 12-13 0.09312374605347173 1107 14-15 0.16503645458757177 1107 16-17 0.31286286417540765 1107 18-19 0.31050380161948254 1107 20-21 0.3801610716349586 1107 22-23 0.30131960816843417 1107 24-25 0.3751873346432433 1107 26-27 0.29228133800279466 1107 28-29 0.23770532498739527 1107 30-31 0.29442667436806147 1107 32-33 0.4337017518177291 1107 34-35 0.46457320928483625 1107 36-37 0.43177146588367066 1107 38-39 0.1571725494198617 1107 40-41 0.13902103168209123 1107 42-43 0.3005997719987832 1107 44-45 0.25100792937492145 1107 46-47 0.357703321544804 1107 48-49 0.20089701754679368 1107 50-51 0.3017859851126161 1107 52-53 0.6265338215139877 1107 54-55 0.4087697206471077 1107 56-57 0.40718905268551 1107 58-59 0.527456669400344 1107 60-61 0.6869579674643731 1107 62-63 0.49171144331663186 1107 64-65 0.8649964653362545 1107 66-67 0.6521091525086113 1107 68-69 0.8660558842754469 1107 70-71 0.9843460504172867 1107 72-73 0.9474893651734995 1107 74-75 0.6162040448923705 1107 76-77 0.876039293279419 1107 78-79 0.8164681010225934 1107 80-81 0.5704603337709173 1107 82-83 0.3002935472148458 1107 84-85 0.4208632880529244 1107 86-87 0.7820936854082383 1107 88-89 1.0294603929014379 1107 90-91 0.541745131558482 1107 92-93 1.0050254477694658 1107 94-95 1.2136412707525572 1107 96-97 1.131129389290944 1107 98-99 0.7404498105798396 1107 100-101 1.6630330039602867 1108 1 0.07902061685303607 1108 2 0.04369670610326892 1108 3 0.08931131529365643 1108 4 -0.01571225878416982 1108 5 0.08042508991111674 1108 6 0.01516962330713767 1108 7 -0.12577417300479254 1108 8 -0.13701677860004224 1108 9 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-0.6637218963552272 2316 58-59 -1.0203979627670137 2316 60-61 -0.9329766882393145 2316 62-63 -1.3790787271669487 2316 64-65 -1.17362170409438 2316 66-67 -1.2503157092928454 2316 68-69 -0.65313706721183 2316 70-71 -0.8399211489137848 2316 72-73 -1.1274939890638116 2316 74-75 -0.6687172167790152 2316 76-77 -0.8412692030275402 2316 78-79 -0.7234585027772837 2316 80-81 -0.842029407793941 2316 82-83 -0.7655462508681801 2316 84-85 -0.5591772811547351 2316 86-87 -0.5419567318971623 2316 88-89 -0.6864699890994572 2316 90-91 -0.1936801483225956 2316 92-93 0.17320184640064085 2316 94-95 0.05888987328271256 2316 96-97 -0.3625142520277116 2316 98-99 -0.201444864324543 2316 100-101 0.9020738983409871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 6.0 6 15.0 7 43.0 8 76.0 9 140.0 10 320.0 11 577.0 12 1019.0 13 1601.0 14 1955.0 15 3101.0 16 4764.0 17 7788.0 18 6977.0 19 3169.0 20 2461.0 21 2431.0 22 2462.0 23 2512.0 24 2865.0 25 3317.0 26 3579.0 27 4018.0 28 4897.0 29 5754.0 30 7118.0 31 8727.0 32 11305.0 33 15085.0 34 21827.0 35 33702.0 36 49026.0 37 72409.0 38 55868.0 39 4700.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.082147529785 20.3606039203881 16.716935474533688 17.840313075293217 2 15.592738762094346 23.447988519050046 40.19200499976853 20.76726771908708 3 18.53039211147632 29.689308365353455 32.0129855099301 19.767314013240128 4 11.672781352715152 19.72535993703995 38.97620480533309 29.625653904911808 5 11.409838721811036 40.210755351345725 33.65488666547073 14.724519261372516 6 26.873755844636822 40.113015601129575 17.3119878709319 15.701240683301698 7 24.689539836118698 37.77024211842044 21.325401601777696 16.214816443683162 8 26.463763251701312 34.1260242581362 19.691507337623257 19.718705152539233 9 28.21889032915143 17.098456089995835 20.751932780889774 33.93072079996296 10-11 23.618408869959723 29.21305726586732 26.258766955233554 20.9097669089394 12-13 26.810283713595396 27.54423530433021 27.569119923610007 18.07636105846439 14-15 23.685680061108283 27.382268182028607 28.794818526920054 20.137233229943057 16-17 18.747251284662745 31.157267024674784 29.507748483866486 20.58773320679598 18-19 18.456176797370492 27.64643419286144 35.60512244803481 18.292266561733253 20-21 20.208584395086447 26.535702247150372 35.86876167301524 17.386951684747935 22-23 19.86675732762362 26.476519776626834 35.57333410491594 18.0833887908336 24-25 19.3752604046109 26.82673834544697 36.036960094440076 17.76104115550206 26-27 19.32851767245308 26.86985418472057 36.109788966417206 17.691839176409143 28-29 18.70127126920033 26.450414424789386 37.133010898453264 17.715303407557013 30-31 18.698090797432407 27.44046490970457 36.335465278611636 17.525979014251387 32-33 18.51681999215892 27.978924556335 35.63092511765242 17.87333033385366 34-35 18.849387759825934 27.018425072913292 36.04289153279941 18.089295634461365 36-37 19.380323827600574 27.280855053006803 35.307537845470115 18.031283273922504 38-39 19.106320309244943 27.308776214064164 35.7816189065321 17.80328457015879 40-41 18.65495231702236 27.72238553770659 35.858148928290355 17.764513216980696 42-43 18.7065597962904 28.24488555803119 34.83766891403108 18.210885731647327 44-45 18.992610295819638 28.300194435442805 34.02996389056062 18.677231378176938 46-47 19.05959214851164 28.901150409703252 32.9487060784223 19.090551363362806 48-49 19.52108698671358 29.034824776630714 32.55636313133651 18.8877251053192 50-51 20.17036248321837 29.343693579000973 31.580424517383456 18.905519420397205 52-53 19.5996423776677 29.772203833155874 31.547873941021248 19.080279848155175 54-55 19.17894426183973 29.817919309291234 31.659847923707236 19.343288505161798 56-57 19.800298597287163 30.33511180037961 30.529836581639742 19.334753020693487 58-59 19.562896393685477 29.33689412527198 31.362407990370816 19.73780149067173 60-61 19.716679783343363 29.592379982408225 31.474671311513358 19.216268922735058 62-63 20.233293597518635 29.470134484514602 31.577386463589647 18.71918545437711 64-65 20.069383361881393 29.181808712559604 31.88307254293783 18.865735382621175 66-67 19.56578977825101 29.791300171288366 31.598363501689736 19.044546548770892 68-69 19.699030137493633 29.923817184389613 31.39134183602611 18.985810842090643 70-71 19.56347507059858 30.035935836303874 31.151769594000278 19.248819499097262 72-73 19.658812092032775 29.53928637563076 30.701269617147354 20.100631915189112 74-75 19.64665987685755 30.037093190130086 30.546618212119807 19.769628720892552 76-77 19.671253645664553 29.19511828156104 31.56205152539234 19.571576547382065 78-79 19.287590852275358 28.89362760983288 31.697895930743947 20.120885607147816 80-81 19.16447733901208 30.606800611082818 30.6345771029119 19.594144946993193 82-83 19.059041044166133 30.283942705115948 30.26643770317014 20.39057854754778 84-85 18.94255474283598 29.249224572936438 30.305165270126384 21.503055414101198 86-87 19.309001898060274 29.673105411786494 30.295327762603584 20.72256492754965 88-89 19.563041062913754 28.578393361418453 30.826553168834774 21.03201240683302 90-91 18.97193057080727 28.512006296487595 31.2564473168311 21.259615815874035 92-93 19.141207072650623 28.825593944672217 30.865447769615145 21.167751213062008 94-95 18.68822623952595 28.713803759085227 30.944892597564927 21.653077403823897 96-97 19.15029975464099 28.76935674274339 30.0333317901949 22.047011712420723 98-99 19.297166938404093 29.112843607997906 29.71495780715853 21.875031646439467 100-101 19.199339076385293 29.372759182468737 30.221774018525537 21.20612772262043 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1474.0 1 1001.5 2 586.0 3 678.0 4 604.0 5 556.5 6 900.0 7 1880.0 8 2924.5 9 3286.0 10 2756.5 11 2079.0 12 1940.5 13 1916.5 14 1917.5 15 1856.0 16 1755.0 17 1763.0 18 1788.0 19 1765.0 20 1803.0 21 1851.5 22 1937.5 23 2145.5 24 2358.5 25 2635.0 26 2925.0 27 3176.0 28 3416.0 29 4092.0 30 4926.0 31 5481.5 32 6599.5 33 7952.0 34 8954.0 35 9753.0 36 11250.5 37 12226.0 38 12287.0 39 12136.5 40 11619.5 41 11158.0 42 10428.5 43 10520.5 44 11147.5 45 11207.5 46 10734.5 47 10844.5 48 14882.5 49 14606.5 50 10263.5 51 8606.0 52 7156.5 53 6667.5 54 6223.0 55 5777.0 56 5398.0 57 4821.0 58 4312.0 59 3995.0 60 3544.0 61 2825.0 62 2186.0 63 1695.0 64 1179.5 65 868.5 66 641.0 67 490.0 68 349.5 69 208.0 70 147.5 71 103.0 72 98.0 73 94.5 74 66.0 75 55.5 76 44.5 77 18.5 78 14.0 79 11.5 80 4.0 81 3.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04050738391741123 2 0.0 3 0.0 4 0.0 5 5.786769131058747E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006076107587611685 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.4466922827646868E-4 22-23 0.001736030739317624 24-25 0.0 26-27 0.00217003842414703 28-29 0.004774084533123467 30-31 1.4466922827646868E-4 32-33 1.4466922827646868E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.007522799870376372 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.4466922827646868E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007667469098652841 92-93 8.68015369658812E-4 94-95 0.0 96-97 0.0 98-99 1.4466922827646868E-4 100-101 0.011284199805564558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 345616.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.82157353364018 #Duplication Level Percentage of deduplicated Percentage of total 1 77.27873176198673 36.95590553542304 2 11.99316773841738 11.470643058080157 3 4.172285362474835 5.985757537947626 4 1.8677474362809265 3.572744854655051 5 1.0601043621625585 2.5347929354244756 6 0.70171513383332 2.013427312336696 7 0.46832903167101386 1.5677361858195724 8 0.3413543907314961 1.3059283277917748 9 0.2445847561455925 1.0526785111101513 >10 1.6635935625118579 15.222173387969587 >50 0.13526840631609996 4.463436147202348 >100 0.0645876174302099 5.794774426126981 >500 0.0036559028734081075 1.1597124863471304 >1k 0.0036559028734081075 3.376569922560439 >5k 0.0012186342911360358 3.523719371204987 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6585 1.9052937364010925 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5508 1.593676218693579 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4012 1.1608258876903845 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2465 0.7132192954029906 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1576 0.45599740752742923 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1324 0.3830841164760891 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1175 0.3399726864497014 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.29975464098884314 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 919 0.2659020415721494 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 902 0.2609832878107495 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 572 0.16550159714828017 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 556 0.1608721818434332 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 526 0.15219202814684504 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 505 0.14611592055923336 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 493 0.14264385908059812 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 459 0.13280635155779824 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 448 0.12962362853571593 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 431 0.12470487477431601 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 406 0.11747141336049256 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 389 0.11255265959909262 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 375 0.10850192120735153 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 371 0.10734456738113976 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 366 0.10589787509837507 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 362 0.10474052127216332 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 349 0.10097912133697515 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4466922827646868E-4 0.0 12-13 0.0 0.0 0.0 2.8933845655293737E-4 0.0 14-15 0.0 0.0 0.0 2.8933845655293737E-4 0.0 16-17 0.0 0.0 0.0 5.786769131058747E-4 0.0 18-19 0.0 0.0 0.0 5.786769131058747E-4 0.0 20-21 0.0 0.0 0.0 7.233461413823435E-4 0.0 22-23 0.0 0.0 0.0 0.0020253691958705615 0.0 24-25 0.0 0.0 0.0 0.00434007684829406 0.0 26-27 0.0 0.0 0.0 0.005063422989676404 0.0 28-29 0.0 0.0 0.0 0.008390815240035184 0.0 30-31 0.0 0.0 0.0 0.015913615110411554 0.0 32-33 0.0 0.0 0.0 0.027197814915976115 0.0 34-35 0.0 0.0 0.0 0.038192676264987735 0.0 36-37 0.0 0.0 0.0 0.053382945234016946 0.0 38-39 0.0 0.0 0.0 0.07407064487755197 0.0 40-41 0.0 0.0 0.0 0.09736239063006341 0.0 42-43 0.0 0.0 0.0 0.11616939030600434 0.0 44-45 0.0 0.0 0.0 0.12875561316605713 0.0 46-47 0.0 0.0 0.0 0.14886463589648627 0.0 48-49 0.0 0.0 0.0 0.168539650942086 0.0 50-51 0.0 0.0 0.0 0.18691264293319754 0.0 52-53 0.0 0.0 0.0 0.2222119346326559 0.0 54-55 0.0 0.0 0.0 0.2891937873246609 0.0 56-57 0.0 0.0 0.0 0.3981297162168418 0.0 58-59 0.0 0.0 0.0 0.5417862598953752 0.0 60-61 0.0 0.0 0.0 0.7260948567195964 0.0 62-63 0.0 0.0 0.0 0.8285206703393362 0.0 64-65 0.0 0.0 0.0 0.9504768297763992 0.0 66-67 0.0 0.0 0.0 1.1932317948243139 0.0 68-69 0.0 0.0 0.0 1.5126614508587566 0.0 70-71 0.0 0.0 0.0 1.891550159714828 0.0 72-73 0.0 0.0 0.0 2.3228091292069815 0.0 74-75 0.0 0.0 0.0 2.7403245220128696 0.0 76-77 0.0 0.0 0.0 3.1205152539234295 0.0 78-79 0.0 0.0 0.0 3.452531132817925 0.0 80-81 0.0 0.0 0.0 3.7075829822693396 0.0 82-83 0.0 0.0 0.0 4.073596129808806 0.0 84-85 0.0 0.0 0.0 4.7727825100689785 0.0 86-87 0.0 0.0 0.0 5.672625109948614 0.0 88-89 0.0 0.0 0.0 6.56624693301236 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 25 0.005018827 56.998528 6 CTTACAC 65 2.5845566E-6 43.84502 3 CTGGTCG 125 1.3460522E-10 38.004513 9 GACGTCG 30 0.0039503723 31.66585 46-47 TATAGGG 125 2.147026E-7 30.399214 2 AGGGGTC 125 2.147026E-7 30.399214 5 GTATGGT 110 2.7174956E-6 30.239614 1 TGTATAC 95 3.484473E-5 29.999224 3 GTATACT 80 4.4878913E-4 29.686731 4 GGTCCTA 115 3.850728E-6 28.916477 8 GGTACTC 100 4.9392227E-5 28.503387 8 GTCGGGA 70 0.008378738 27.14608 8 GTGGTAC 105 6.893314E-5 27.142157 6 GGTATCA 4755 0.0 26.283073 1 ATGGGAG 530 0.0 25.093689 5 GTATCAA 6385 0.0 24.708597 1 GGTGGTA 135 1.3394654E-5 24.628992 5 GTACATG 2980 0.0 24.557003 1 TGGGATA 195 1.5621481E-8 24.358343 6 AGTACTT 4420 0.0 23.80656 12-13 >>END_MODULE