FastQCFastQC Report
Thu 26 May 2016
SRR937330_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937330_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337722
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55881.6546153345058954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43881.2992935017558822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32450.9608494560614944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14640.43349263595501625No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13230.3917423206068897No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11630.34436607624022125No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT10330.30587287769230315No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.2650108669260516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.2605693440166764No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG7150.2117125920135496No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.1835829469208402No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT5760.1705544797200064No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5190.15367669266438078No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA5180.1533805911370891No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.15160398197333902No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4890.14479364684563042No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.12169772771687956No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3950.11696010328021272No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG3440.10185892538833714No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG3380.10008231622458709No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCC608.2529856E-539.603885
GTATGGT500.001610083938.0197221
GGTATGC550.002574243834.5531238
AGTCCGC300.003945512731.67369510-11
GGCCGAT600.003942735531.6736957
GTATCAA52200.029.7711931
GTACATG29400.029.5816731
CATGGGG6650.028.5820144
GGTATCA35800.028.4085921
TACATGG31050.027.8442362
ATGGGAG5850.027.6211685
TATACGT700.008362127.1569445
TATATGG700.00838029627.144852
CGGTGTG350.00839525827.13880520-21
ACATGGG29700.027.0306033
TTAGGAG908.9305366E-426.3908273
TCTAGAC908.9305366E-426.3908273
ATGGGGG3100.026.0619075
GAGTACT34500.025.95480312-13
CTTACTA1109.442876E-525.9148439
Produced by FastQC (version 0.11.5)