##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937330_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 337722 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02742196244248 33.0 31.0 34.0 31.0 34.0 2 32.127684900598716 33.0 31.0 34.0 31.0 34.0 3 32.11770331811371 34.0 31.0 34.0 30.0 34.0 4 35.743087509845374 37.0 35.0 37.0 35.0 37.0 5 35.764996061849686 37.0 35.0 37.0 35.0 37.0 6 35.85521227518492 37.0 36.0 37.0 35.0 37.0 7 35.817216527202845 37.0 35.0 37.0 35.0 37.0 8 35.831666281734684 37.0 35.0 37.0 35.0 37.0 9 37.47344265401721 39.0 37.0 39.0 35.0 39.0 10-11 37.383439633781634 39.0 37.0 39.0 35.0 39.0 12-13 37.263876798076524 39.0 37.0 39.0 34.5 39.0 14-15 38.372996132914054 40.0 38.0 41.0 34.0 41.0 16-17 38.3895215591522 40.0 38.0 41.0 34.0 41.0 18-19 38.48424443773281 40.0 38.0 41.0 34.5 41.0 20-21 38.49881411338319 40.0 38.0 41.0 34.0 41.0 22-23 38.354680772943425 40.0 38.0 41.0 34.0 41.0 24-25 38.20416200306761 40.0 38.0 41.0 34.0 41.0 26-27 38.01313950527357 40.0 38.0 41.0 34.0 41.0 28-29 38.017640248488405 40.0 38.0 41.0 34.0 41.0 30-31 37.74766968098021 40.0 37.0 41.0 34.0 41.0 32-33 37.434283227032886 39.5 36.0 41.0 33.0 41.0 34-35 37.237845032304676 39.5 35.0 41.0 33.0 41.0 36-37 37.17635658914729 40.0 35.0 41.0 33.0 41.0 38-39 37.03968204617999 40.0 35.0 41.0 33.0 41.0 40-41 36.80016255973848 39.5 35.0 41.0 32.5 41.0 42-43 36.47007746015954 39.0 35.0 41.0 31.0 41.0 44-45 36.08010434617822 39.0 35.0 41.0 30.5 41.0 46-47 35.49931452496432 39.0 35.0 41.0 28.0 41.0 48-49 34.99141749723145 39.0 35.0 41.0 24.0 41.0 50-51 34.04907290611805 38.0 34.0 40.0 22.5 40.5 52-53 34.09304990495141 38.0 34.5 40.0 22.0 40.5 54-55 34.38999976311878 38.5 35.0 40.0 21.5 41.0 56-57 34.201190624241235 38.0 35.0 40.0 19.5 41.0 58-59 33.982737280958894 38.0 34.5 40.0 18.0 41.0 60-61 33.68587477274208 38.0 34.0 40.0 13.5 41.0 62-63 33.325218670977904 37.5 34.0 40.0 8.0 41.0 64-65 32.92590799533344 37.0 33.0 40.0 4.5 41.0 66-67 32.54822309473472 36.0 33.0 39.5 2.0 41.0 68-69 32.09028135567123 36.0 33.0 39.0 2.0 41.0 70-71 31.620508880084806 35.0 32.0 39.0 2.0 40.0 72-73 31.182638383048783 35.0 31.5 38.0 2.0 40.0 74-75 30.70055992798811 35.0 31.0 37.0 2.0 39.5 76-77 30.24069056798195 35.0 31.0 37.0 2.0 39.0 78-79 29.770377707108214 35.0 30.0 36.0 2.0 39.0 80-81 29.290103990856387 34.5 29.5 36.0 2.0 37.5 82-83 28.81374177578008 34.0 29.0 35.0 2.0 37.0 84-85 28.371438638880498 34.0 29.0 35.0 2.0 37.0 86-87 27.919140594927185 34.0 28.0 35.0 2.0 36.0 88-89 27.48256554207307 34.0 26.5 35.0 2.0 36.0 90-91 27.059702950947823 34.0 26.0 35.0 2.0 35.5 92-93 26.62154375492269 33.5 25.0 35.0 2.0 35.0 94-95 26.121483054109593 33.0 24.5 35.0 2.0 35.0 96-97 25.527953464683968 33.0 21.5 35.0 2.0 35.0 98-99 24.84918364808926 32.5 10.0 35.0 2.0 35.0 100-101 23.107779475426533 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12879600926802937 1101 2 0.026420400198567506 1101 3 -0.05446460022655586 1101 4 -0.07745360960890935 1101 5 -0.04173430387762522 1101 6 -0.1874237385467552 1101 7 -0.09753938230512915 1101 8 0.06468740155438724 1101 9 0.15521136202358576 1101 10-11 -0.021456152272406825 1101 12-13 -0.1112938299310855 1101 14-15 -0.06800137398091977 1101 16-17 0.1545339570689208 1101 18-19 -0.002855689820684404 1101 20-21 0.006702506782318096 1101 22-23 0.09637919940189477 1101 24-25 -0.14394781591215633 1101 26-27 -0.18872642769637338 1101 28-29 -0.3983307704874477 1101 30-31 -0.032399967403435426 1101 32-33 0.18035528772853837 1101 34-35 0.08577943982785285 1101 36-37 0.026270680916823608 1101 38-39 0.03552638659691354 1101 40-41 0.035431987889161576 1101 42-43 0.09808131947167453 1101 44-45 -0.11677557759396251 1101 46-47 -0.11492840105125168 1101 48-49 0.37009475947306925 1101 50-51 0.48626154792329146 1101 52-53 0.48507280683072196 1101 54-55 0.4685441354343709 1101 56-57 0.6207377306531541 1101 58-59 0.8181117252446555 1101 60-61 0.6871534373300463 1101 62-63 0.7077235490842568 1101 64-65 0.5189725665222156 1101 66-67 0.5641100337245533 1101 68-69 0.9320704045897124 1101 70-71 0.9717476717477282 1101 72-73 0.717936002598826 1101 74-75 0.8190781234980875 1101 76-77 0.8877599125970299 1101 78-79 0.8051994505300399 1101 80-81 1.2052918668869204 1101 82-83 1.1129233892723072 1101 84-85 1.079574392814152 1101 86-87 0.9538132863235802 1101 88-89 0.8793698473046092 1101 90-91 1.0247824897813835 1101 92-93 0.86202581651062 1101 94-95 1.4039158675378012 1101 96-97 1.399869873221892 1101 98-99 1.268451656607521 1101 100-101 1.536133776028631 1102 1 0.014278016531150683 1102 2 0.02652769633590424 1102 3 0.11927260677245499 1102 4 -0.11240738973436493 1102 5 0.06312703649873441 1102 6 -0.10716622781714591 1102 7 -0.06705902575181 1102 8 -0.08418186222903756 1102 9 -4.4231905927460957E-4 1102 10-11 0.0787295525806826 1102 12-13 -0.07951353940616457 1102 14-15 0.2260065494261454 1102 16-17 0.10714620533567398 1102 18-19 0.08496619859133148 1102 20-21 0.10312046650499695 1102 22-23 0.2035432799565342 1102 24-25 0.31941880673558387 1102 26-27 0.21925062743734003 1102 28-29 0.11207695483444269 1102 30-31 0.3072211946962682 1102 32-33 0.40089837125445626 1102 34-35 0.3308231836376976 1102 36-37 0.3980022755024919 1102 38-39 0.5963013948504567 1102 40-41 0.932675302514447 1102 42-43 0.4249012270319241 1102 44-45 0.23794957922347493 1102 46-47 0.5193155738579591 1102 48-49 0.055758344814762495 1102 50-51 0.2632011319444274 1102 52-53 0.5242441505085083 1102 54-55 0.49569005818114675 1102 56-57 0.6513006226967306 1102 58-59 0.5325843234618901 1102 60-61 0.5035656195684126 1102 62-63 0.576327048589043 1102 64-65 0.5504227150860928 1102 66-67 0.5976978514861884 1102 68-69 0.4362054326517786 1102 70-71 0.3129576420349487 1102 72-73 0.4405177127341844 1102 74-75 0.5557403882719392 1102 76-77 0.6879579977736583 1102 78-79 0.44607102530719445 1102 80-81 0.26409427692323817 1102 82-83 0.5505967138018555 1102 84-85 0.5648624416751602 1102 86-87 0.47125303541568897 1102 88-89 0.6634198638117077 1102 90-91 0.4946240216400817 1102 92-93 0.5828907884485552 1102 94-95 0.6264109154083854 1102 96-97 0.9752082687319579 1102 98-99 1.1079779028934276 1102 100-101 1.3977754069299166 1103 1 0.29593807772588576 1103 2 0.35811896479186345 1103 3 0.2111776522578026 1103 4 0.3311312266075035 1103 5 0.2157647759898751 1103 6 0.23171501743410516 1103 7 0.2983844307239494 1103 8 0.3208430879732376 1103 9 0.2471602026566373 1103 10-11 0.32654255954209077 1103 12-13 0.2538745543162406 1103 14-15 0.36930079753177836 1103 16-17 0.25198058938397594 1103 18-19 0.0737591427151969 1103 20-21 -0.02471081673626685 1103 22-23 0.2032249851950496 1103 24-25 0.13935637989204253 1103 26-27 0.18168671179571305 1103 28-29 0.42595152616326715 1103 30-31 0.4194817035571816 1103 32-33 0.25841012762548843 1103 34-35 0.614151725436983 1103 36-37 0.8309909606371022 1103 38-39 0.676265252993673 1103 40-41 0.9023808285222117 1103 42-43 1.0142342454930215 1103 44-45 1.0862102523655537 1103 46-47 1.2748857321839324 1103 48-49 1.2157425327524223 1103 50-51 1.205271485354288 1103 52-53 1.035661691630139 1103 54-55 0.9983288244295849 1103 56-57 1.309631731389267 1103 58-59 1.1506614123996073 1103 60-61 1.1247178466049803 1103 62-63 1.0061225185358538 1103 64-65 1.115370247788313 1103 66-67 1.1834834234926745 1103 68-69 1.3626280048841544 1103 70-71 1.2573844034370865 1103 72-73 0.9822348616345238 1103 74-75 1.088971388128197 1103 76-77 1.5597878846250062 1103 78-79 1.274227790611011 1103 80-81 1.456842290147872 1103 82-83 1.071522027476206 1103 84-85 1.3082963287729257 1103 86-87 1.4348493555175459 1103 88-89 1.1199982978014802 1103 90-91 1.4481170499580465 1103 92-93 1.5368509913357933 1103 94-95 1.4148424409643745 1103 96-97 1.1309519490408064 1103 98-99 1.5389798126619958 1103 100-101 1.8742318621530316 1104 1 0.26871723262298985 1104 2 0.09599871345157851 1104 3 0.1286215906909618 1104 4 0.039668725886919276 1104 5 0.10930832818910119 1104 6 0.13305818916408896 1104 7 -0.03861908573422568 1104 8 -0.07588257064208648 1104 9 0.18456724710236472 1104 10-11 0.08316618322342606 1104 12-13 0.22094667053229955 1104 14-15 0.21851391795605934 1104 16-17 0.1967837253729101 1104 18-19 0.291572021022688 1104 20-21 0.39613716850613656 1104 22-23 0.31148577956872003 1104 24-25 0.46060501077922567 1104 26-27 0.5390655092823167 1104 28-29 0.4935366993920738 1104 30-31 0.5355680585465237 1104 32-33 0.2468288743828495 1104 34-35 0.6529365297259133 1104 36-37 0.5465440822143179 1104 38-39 0.552359102074206 1104 40-41 0.7524384753775806 1104 42-43 0.8445247856440616 1104 44-45 0.7391905947878783 1104 46-47 0.6092374932953675 1104 48-49 0.6002384110022589 1104 50-51 0.3490975071447906 1104 52-53 0.22441479014861443 1104 54-55 0.4995682318199144 1104 56-57 0.30560798088485086 1104 58-59 0.4571409189425282 1104 60-61 0.34835593964700706 1104 62-63 0.1104575620592314 1104 64-65 0.067895180053263 1104 66-67 0.17820873622687117 1104 68-69 0.3722233239594992 1104 70-71 0.4372944743427212 1104 72-73 0.48591561334951905 1104 74-75 0.4478547685397949 1104 76-77 0.8355819700576426 1104 78-79 0.7158574672121532 1104 80-81 0.7166797260157445 1104 82-83 0.9431713040081746 1104 84-85 1.0505312973553913 1104 86-87 0.9084240370186656 1104 88-89 0.560475037295344 1104 90-91 0.5529103859815656 1104 92-93 1.3257885597914374 1104 94-95 1.3688950148964665 1104 96-97 1.4544574543154951 1104 98-99 1.4208844647632226 1104 100-101 1.4408024507274249 1105 1 0.019937070422940906 1105 2 -0.06520009841639407 1105 3 -0.1432670719930158 1105 4 -0.131976302982018 1105 5 -0.1343989107389092 1105 6 0.13778401768593085 1105 7 0.1425042997745649 1105 8 0.26770785755268633 1105 9 -0.205874297469272 1105 10-11 0.010690129423736039 1105 12-13 -0.06921786572908672 1105 14-15 0.21291701510921257 1105 16-17 0.0851460388929155 1105 18-19 -0.12719779508384477 1105 20-21 0.19796609084368555 1105 22-23 0.1315805631351168 1105 24-25 0.3998701043946866 1105 26-27 0.16626238542956173 1105 28-29 -0.07984046577830384 1105 30-31 0.014608875554159795 1105 32-33 0.012376382976782452 1105 34-35 0.24384197978098143 1105 36-37 -0.08275872458072797 1105 38-39 -0.013485439527102017 1105 40-41 0.17857070512760487 1105 42-43 0.2257049077947073 1105 44-45 0.3265149615068239 1105 46-47 0.5085800157397102 1105 48-49 0.11394197873580225 1105 50-51 0.3356542604739019 1105 52-53 0.5185375415419955 1105 54-55 0.3217989830797947 1105 56-57 0.44612128062119183 1105 58-59 0.2769520192996282 1105 60-61 0.5731565270381225 1105 62-63 0.5024710453552999 1105 64-65 0.6573621893647399 1105 66-67 0.5004061129915485 1105 68-69 0.8450631597569789 1105 70-71 0.7811819355448719 1105 72-73 0.9227410313650708 1105 74-75 1.017767661107296 1105 76-77 1.0130742104875061 1105 78-79 1.0145324997522849 1105 80-81 0.826144732857518 1105 82-83 0.7860775550511292 1105 84-85 0.8235947422707852 1105 86-87 1.046030418222152 1105 88-89 0.8780567213723707 1105 90-91 1.127605843712768 1105 92-93 1.2647532829500001 1105 94-95 0.938954755242893 1105 96-97 0.7579350767164534 1105 98-99 0.8182545545406121 1105 100-101 0.8102681250590997 1106 1 -0.042170219625031535 1106 2 -0.09431130711850955 1106 3 -0.09620944075188476 1106 4 -0.05517405989034074 1106 5 0.14447582745445686 1106 6 0.09367382242885469 1106 7 0.05541558955228254 1106 8 -0.20121316129739597 1106 9 -0.02199314079066994 1106 10-11 -0.4479570903587131 1106 12-13 -0.43500396126317753 1106 14-15 -0.29776216197341654 1106 16-17 -0.43765647445077605 1106 18-19 0.02275406627686749 1106 20-21 -0.20593820616327463 1106 22-23 -0.07010766738984842 1106 24-25 -0.18520016112790927 1106 26-27 -0.5245144201916787 1106 28-29 -0.13064784365817417 1106 30-31 -0.2610819561463984 1106 32-33 0.26088999692178305 1106 34-35 0.06650176628189541 1106 36-37 0.2521384107479605 1106 38-39 0.3389738537638891 1106 40-41 -0.23262617346168213 1106 42-43 -0.5296156786521564 1106 44-45 -0.3418224819091549 1106 46-47 -0.012676993921800772 1106 48-49 0.10133303339426902 1106 50-51 0.2315945685611922 1106 52-53 0.33436924210277397 1106 54-55 0.19171486714169106 1106 56-57 0.082205835718824 1106 58-59 0.33867926745854504 1106 60-61 0.06311497579158498 1106 62-63 -0.19366012821405576 1106 64-65 0.19901478040588927 1106 66-67 0.15552654404350363 1106 68-69 0.15976740271354117 1106 70-71 0.18537337531245512 1106 72-73 0.2881048581335328 1106 74-75 0.25695335801966124 1106 76-77 0.7217889932724546 1106 78-79 0.759866429892142 1106 80-81 0.7555498406205068 1106 82-83 0.5380875543754939 1106 84-85 0.42361754478412905 1106 86-87 0.4028517478620408 1106 88-89 0.8194964889556395 1106 90-91 0.7474372740215323 1106 92-93 0.9980483305819163 1106 94-95 0.9543380063745737 1106 96-97 1.1222432315708595 1106 98-99 1.3048728573579886 1106 100-101 1.3477191418822905 1107 1 0.34123108223364795 1107 2 0.20222425871946825 1107 3 0.29917131369874994 1107 4 0.15600545544179312 1107 5 0.28843512476447586 1107 6 0.32511645437929104 1107 7 0.3598622996142353 1107 8 0.17550239042404314 1107 9 0.4599788614052329 1107 10-11 0.5123646491126976 1107 12-13 0.4343004070216665 1107 14-15 0.5545190316584581 1107 16-17 0.4330987604327561 1107 18-19 0.2575542780249762 1107 20-21 0.42733075313403646 1107 22-23 0.6869555041261108 1107 24-25 0.6367255701872807 1107 26-27 0.494018439976152 1107 28-29 0.5589843420527458 1107 30-31 0.7386827681889514 1107 32-33 0.836903642910336 1107 34-35 0.8078700903349088 1107 36-37 0.8890149302366268 1107 38-39 0.7528433541952282 1107 40-41 1.0934953072065028 1107 42-43 1.1697020219203509 1107 44-45 1.4341117585425707 1107 46-47 1.6600839660029152 1107 48-49 1.670768763925203 1107 50-51 1.1807211076561686 1107 52-53 1.0443233633481626 1107 54-55 1.4887209843089622 1107 56-57 1.6031208619912718 1107 58-59 1.8181024499540328 1107 60-61 1.9453497024796818 1107 62-63 1.5554232770090621 1107 64-65 1.5482422919736152 1107 66-67 1.6060745402790957 1107 68-69 1.6821136892793014 1107 70-71 1.8119467813198327 1107 72-73 1.6890341970089153 1107 74-75 1.3957414922836442 1107 76-77 1.6049501178901302 1107 78-79 2.0856595049450775 1107 80-81 2.0426743798723273 1107 82-83 2.209878002183512 1107 84-85 1.749822352250213 1107 86-87 1.7977472462543211 1107 88-89 1.797243332340475 1107 90-91 1.942059264453654 1107 92-93 1.9310339787663722 1107 94-95 2.542071321408688 1107 96-97 1.6006860987969596 1107 98-99 1.699189969741333 1107 100-101 2.545257570240846 1108 1 0.03912409970794073 1108 2 0.20062167815383702 1108 3 0.2363316642118818 1108 4 0.22093051425444798 1108 5 0.058320992263936944 1108 6 0.19979670873758693 1108 7 0.1419113426887293 1108 8 0.2267849685241643 1108 9 -0.03131481148341919 1108 10-11 0.14589762015324936 1108 12-13 0.20186972376760792 1108 14-15 0.39907898974480815 1108 16-17 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-2.8275828204664357 2316 94-95 -2.711553093325893 2316 96-97 -2.9949119531388853 2316 98-99 -3.4373625584172203 2316 100-101 -3.4253428321585027 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1285.0 3 206.0 4 168.0 5 176.0 6 184.0 7 229.0 8 273.0 9 346.0 10 459.0 11 738.0 12 1245.0 13 1840.0 14 1970.0 15 2836.0 16 4226.0 17 6138.0 18 5057.0 19 2798.0 20 2387.0 21 2456.0 22 2621.0 23 2784.0 24 2954.0 25 3306.0 26 3683.0 27 4226.0 28 4786.0 29 5716.0 30 6774.0 31 8476.0 32 10786.0 33 14464.0 34 20445.0 35 31737.0 36 45019.0 37 65369.0 38 60358.0 39 9200.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25436081491011 20.037911087210354 16.05635560362116 19.65137249425837 2 14.37060839505586 22.19427302047386 41.37983262156913 22.05528596290115 3 17.62539829605015 29.49068588584308 31.628195648365093 21.255720169741675 4 10.887028518312837 18.30867962854623 39.31090395597673 31.493387897164205 5 11.10533646550111 40.15163889392108 34.04896041529931 14.694064225278494 6 26.21893677256999 39.14990474042508 18.934695258387887 15.696463228617045 7 24.695933415426612 36.87461982404344 21.666097948162545 16.7633488123674 8 25.703645454680334 33.45212836985712 20.905651419160524 19.93857475630202 9 27.508946215884205 16.384185820173645 21.24748526767434 34.85938269626781 10-11 23.08353243207147 28.551501031356196 27.331829581373263 21.033136955199076 12-13 25.8420097543894 26.893863518672667 28.208346872853152 19.055779854084783 14-15 22.861674458491656 27.009170663964376 28.996716602596155 21.132438274947813 16-17 18.49707637913153 30.913229069144638 29.508615074637273 21.081079477086558 18-19 18.48230062892522 28.012320346821323 34.72960963313437 18.77576939111908 20-21 19.964484506106174 26.857220000237366 34.471985188525856 18.706310305130607 22-23 19.466130185661594 26.89289073650415 34.35807733278736 19.2829017450469 24-25 19.03182404136992 27.344970353635073 34.774733612910595 18.848471992084413 26-27 18.88406287270591 27.339628911001533 34.76378233084714 19.01252588544541 28-29 18.39022819763776 27.10316147419739 35.6350914882474 18.87151883991745 30-31 18.351470295839725 28.09987743825173 34.964374000290825 18.58427826561772 32-33 18.034056531408893 28.38885039547654 34.30816457640019 19.268928496714377 34-35 18.47559157000684 27.818888395924183 34.49483401021179 19.210686023857185 36-37 18.83635058132031 27.84685247355983 34.0178824423019 19.29891450281796 38-39 18.7232407509057 27.995626541021206 34.375565597434075 18.90556711063902 40-41 18.1115701559846 28.32348076338256 34.76461382128897 18.80033525934387 42-43 18.113802659767018 28.818593444938312 33.517248132169414 19.550355763125257 44-45 18.320091984718665 29.194169355736065 32.923852972812576 19.561885686732687 46-47 18.375746281943243 29.226062598663518 32.130479163452065 20.267711955941177 48-49 18.779322521773967 29.5737198985118 31.56001008946986 20.086947490244373 50-51 19.366881623423065 29.60141497535367 31.210029468761036 19.82167393246223 52-53 18.811607882247134 29.941467874114373 31.3599517970728 19.88697244656569 54-55 18.530806038799657 29.89502577988798 31.507845246485406 20.06632293482696 56-57 19.08198443025657 30.35373104217597 30.30077374029858 20.26351078726889 58-59 18.777108612454384 29.511377458688102 31.19559735366541 20.515916575192097 60-61 19.13684538422173 29.437443911266698 31.36548716541448 20.06022353909709 62-63 19.617360448712148 29.63090429264197 31.211365554726477 19.540369703919406 64-65 19.335789745369752 29.255778353313932 31.697801985896394 19.71062991541992 66-67 18.834113310539706 29.77550006304095 31.27765458011021 20.112732046309137 68-69 18.644623391956856 30.195390313398768 31.285885708606937 19.874100586037446 70-71 18.77451358519899 29.768170463243713 31.140931234386958 20.316384717170337 72-73 18.610584903673132 29.674489809541754 30.620328106202095 21.09459718058302 74-75 18.52488662274488 29.87774393726533 31.03547517568468 20.561894264305117 76-77 18.701766878810805 29.07324165146053 31.662586971679502 20.562404498049165 78-79 18.407041311181626 28.975980560142062 31.870596769814814 20.7463813588615 80-81 18.263897233682407 30.148811775579205 31.090994595540838 20.496296395197554 82-83 18.385432041261645 29.655396455023237 30.693761060437268 21.265410443277847 84-85 18.17521095591322 29.049100183478615 30.691063241534692 22.08462561907347 86-87 18.625820815601838 29.15376822407172 30.8294409331674 21.39097002715904 88-89 19.09259871812177 28.195536120872127 31.064854850504396 21.647010310501702 90-91 18.29208956242342 27.983955426465833 31.798695191671538 21.925259819439212 92-93 18.35940049484096 28.568016898166004 31.5573843442764 21.51519826271664 94-95 17.862816441379636 28.258157724158494 31.53252039152923 22.346505442932642 96-97 17.85661310992975 28.3096698784887 30.891933263717487 22.941783747864058 98-99 18.06284703009733 28.55975185169252 30.771695546592337 22.605705571617815 100-101 18.129540580066983 28.300291605730067 31.529609995876584 22.040557818326366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 903.0 1 618.0 2 418.5 3 528.0 4 473.5 5 511.0 6 923.0 7 1939.5 8 3038.5 9 3307.0 10 2627.5 11 1886.5 12 1762.0 13 1782.5 14 1743.0 15 1679.5 16 1582.0 17 1537.5 18 1526.5 19 1522.0 20 1596.0 21 1743.0 22 1862.0 23 2066.0 24 2276.0 25 2518.0 26 2854.5 27 3220.0 28 3557.5 29 4084.0 30 4885.0 31 5523.5 32 6544.0 33 8043.0 34 9193.5 35 9827.0 36 11119.5 37 12357.0 38 12300.5 39 11724.0 40 11125.0 41 10817.5 42 10341.0 43 10496.0 44 10955.0 45 10988.0 46 10700.5 47 10759.5 48 14595.0 49 14180.5 50 9782.0 51 8278.5 52 7054.5 53 6565.5 54 6278.0 55 5759.5 56 5204.5 57 4668.0 58 4150.5 59 3692.0 60 3302.5 61 2895.0 62 2316.5 63 1615.0 64 1035.0 65 815.5 66 627.0 67 437.0 68 308.0 69 207.0 70 144.5 71 116.5 72 88.5 73 76.0 74 62.5 75 41.0 76 29.0 77 14.5 78 9.0 79 5.0 80 4.0 81 5.5 82 3.5 83 2.5 84 3.0 85 1.5 86 1.0 87 0.5 88 0.0 89 2.0 90 2.0 91 1.5 92 2.5 93 1.0 94 0.0 95 1.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33903624874897104 2 0.29610152729167777 3 0.28869898910938585 4 0.29343661354605266 5 0.29639762881896947 6 0.22148394241417496 7 0.20934377979521618 8 0.21526581034104972 9 0.20963988132250785 10-11 0.2330319019785504 12-13 0.2207436885959458 14-15 0.21422945499552887 16-17 0.21482165805011222 18-19 0.21274894735907046 20-21 0.20312564772209094 22-23 0.21052818590438288 24-25 0.19705556641261154 26-27 0.19542700801250734 28-29 0.2106762366680287 30-31 0.22178004394146664 32-33 0.21422945499552887 34-35 0.2245930084507376 36-37 0.20356980001302846 38-39 0.20401395230396602 40-41 0.19823997252177827 42-43 0.20727106910417445 44-45 0.21008403361344538 46-47 0.21260089659542464 48-49 0.21822682561396653 50-51 0.22355665310521672 52-53 0.24073054168813404 54-55 0.2174865717957373 56-57 0.19483480495792396 58-59 0.1930581957941739 60-61 0.19083743433948633 62-63 0.1979438709944866 64-65 0.19098548510313218 66-67 0.18935692670302792 68-69 0.1858037083755278 70-71 0.19661141412167402 72-73 0.19735166793990325 74-75 0.20238539390386176 76-77 0.2034217492493826 78-79 0.20208929237657008 80-81 0.2029775969584451 82-83 0.19350234808511144 84-85 0.18521150532094446 86-87 0.18758031753927787 88-89 0.18906082517573625 90-91 0.19572310953979902 92-93 0.19068938357584048 94-95 0.18758031753927787 96-97 0.18772836830292372 98-99 0.19705556641261154 100-101 0.18447125150271526 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 337722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.387949875371966 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81392769517369 40.50086165880776 2 11.403990519406063 11.720553863809117 3 3.8355749127253755 5.913069940750975 4 1.7388529019798074 3.5742434307033366 5 0.9697187548346701 2.491592938332607 6 0.5777998444737962 1.7815169667490292 7 0.3947147702807452 1.4198507981179125 8 0.3175049794560316 1.3052743975574046 9 0.24650110070724127 1.1400467586630958 >10 1.5233486266991219 14.647581356744507 >50 0.11871059617565417 4.181366680738571 >100 0.05071132263814353 4.383031745156628 >500 0.00461012023983123 1.5502446581916727 >1k 0.0034575901798734225 3.7359859804672673 >5k 5.762650299789038E-4 1.6547788252101368 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5588 1.6546153345058954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4388 1.2992935017558822 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3245 0.9608494560614944 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1464 0.43349263595501625 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1323 0.3917423206068897 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1163 0.34436607624022125 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1033 0.30587287769230315 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 895 0.2650108669260516 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 880 0.2605693440166764 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 715 0.2117125920135496 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.1835829469208402 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 576 0.1705544797200064 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 519 0.15367669266438078 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 518 0.1533805911370891 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 512 0.15160398197333902 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 489 0.14479364684563042 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 411 0.12169772771687956 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 395 0.11696010328021272 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 344 0.10185892538833714 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 338 0.10008231622458709 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.4805076364583889E-4 0.0 18-19 0.0 0.0 0.0 2.9610152729167777E-4 0.0 20-21 0.0 0.0 0.0 2.9610152729167777E-4 0.0 22-23 0.0 0.0 0.0 5.922030545833555E-4 0.0 24-25 0.0 0.0 0.0 0.002368812218333422 0.0 26-27 0.0 0.0 0.0 0.003849319854791811 0.0 28-29 0.0 0.0 0.0 0.008734995055104494 0.0 30-31 0.0 0.0 0.0 0.01746999011020899 0.0 32-33 0.0 0.0 0.0 0.026945238983542674 0.0 34-35 0.0 0.0 0.0 0.039529553893438985 0.0 36-37 0.0 0.0 0.0 0.052854122621564484 0.0 38-39 0.0 0.0 0.0 0.07032411273177347 0.0 40-41 0.0 0.0 0.0 0.09031096582396173 0.0 42-43 0.0 0.0 0.0 0.10866926051604575 0.0 44-45 0.0 0.0 0.0 0.12421459069885882 0.0 46-47 0.0 0.0 0.0 0.1431650884455262 0.0 48-49 0.0 0.0 0.0 0.15826626633740176 0.0 50-51 0.0 0.0 0.0 0.17425574881115236 0.0 52-53 0.0 0.0 0.0 0.21067623666802873 0.0 54-55 0.0 0.0 0.0 0.2630862069986557 0.0 56-57 0.0 0.0 0.0 0.36420487856876366 0.0 58-59 0.0 0.0 0.0 0.4955259059226227 0.0 60-61 0.0 0.0 0.0 0.6777763959706504 0.0 62-63 0.0 0.0 0.0 0.7735652400495081 0.0 64-65 0.0 0.0 0.0 0.8900811910387834 0.0 66-67 0.0 0.0 0.0 1.102682087634208 0.0 68-69 0.0 0.0 0.0 1.3793889648882809 0.0 70-71 0.0 0.0 0.0 1.7224225842556895 0.0 72-73 0.0 0.0 0.0 2.1317829457364343 0.0 74-75 0.0 0.0 0.0 2.515678575870094 0.0 76-77 0.0 0.0 0.0 2.855751179964586 0.0 78-79 0.0 0.0 0.0 3.1468189812923058 0.0 80-81 0.0 0.0 0.0 3.3607523347605426 0.0 82-83 0.0 0.0 0.0 3.7200715381289937 0.0 84-85 0.0 0.0 0.0 4.419463345591937 0.0 86-87 0.0 0.0 0.0 5.275640911755823 0.0 88-89 0.0 0.0 0.0 6.144254742065959 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCC 60 8.2529856E-5 39.60388 5 GTATGGT 50 0.0016100839 38.019722 1 GGTATGC 55 0.0025742438 34.553123 8 AGTCCGC 30 0.0039455127 31.673695 10-11 GGCCGAT 60 0.0039427355 31.673695 7 GTATCAA 5220 0.0 29.771193 1 GTACATG 2940 0.0 29.581673 1 CATGGGG 665 0.0 28.582014 4 GGTATCA 3580 0.0 28.408592 1 TACATGG 3105 0.0 27.844236 2 ATGGGAG 585 0.0 27.621168 5 TATACGT 70 0.0083621 27.156944 5 TATATGG 70 0.008380296 27.14485 2 CGGTGTG 35 0.008395258 27.138805 20-21 ACATGGG 2970 0.0 27.030603 3 TTAGGAG 90 8.9305366E-4 26.390827 3 TCTAGAC 90 8.9305366E-4 26.390827 3 ATGGGGG 310 0.0 26.061907 5 GAGTACT 3450 0.0 25.954803 12-13 CTTACTA 110 9.442876E-5 25.914843 9 >>END_MODULE