FastQCFastQC Report
Thu 26 May 2016
SRR937330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937330_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337722
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67431.9966125985277832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56381.6694204108704793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42311.2528055619710887No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23620.6993918074629428No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15670.4639910932660591No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12740.3772333457695975No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.3461426854039713No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.2901794967458442No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.27359781121751026No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT9150.27093289747188515No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.18624786066646531No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.1587104186283393No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5220.1545649972462558No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT5170.1530844896097974No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.14420144379104707No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG4610.13650280408146345No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA4520.13383789033583834No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG4260.12613925062625472No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG4120.12199382924417124No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT4100.1214016261895879No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3950.11696010328021272No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA3770.11163027578896252No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3570.10570824524312897No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT853.6280835E-739.1210024
GGTAAGG702.0492412E-433.931483
ACGCCGA352.0531911E-433.92645678-79
GTATAGG1605.2750693E-1132.6832471
CCGCTCG300.003948150731.66938486-87
TATAGGG753.0723333E-431.6693822
GGGACGC300.00395101431.66469676-77
CCGACCG300.00395101431.66469682-83
GACGCCG300.00395101431.66469678-79
GGTGTGC1950.031.6646928
GTAGATA1051.8973406E-631.6646926
ATATAGG1701.07320375E-1030.737932
GGTACTC953.4815843E-530.0025733
GGACTGA953.4851066E-529.9981296
AGGTAAG804.484743E-429.6900442
GTTATAC700.00834959427.1652971
CACTTAG700.00834959427.1652971
GACCGCT350.00838565627.14518484-85
CGATTGC350.00838868627.14317392-93
AGCCCGT350.008391716527.14116544-45
Produced by FastQC (version 0.11.5)