##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937330_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 337722 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39033879936753 33.0 31.0 34.0 31.0 34.0 2 32.577036734355474 34.0 31.0 34.0 31.0 34.0 3 32.60776318984254 34.0 31.0 34.0 31.0 34.0 4 36.220095226251175 37.0 35.0 37.0 35.0 37.0 5 36.13612971615708 37.0 35.0 37.0 35.0 37.0 6 36.26946423389652 37.0 37.0 37.0 35.0 37.0 7 36.233718857521865 37.0 37.0 37.0 35.0 37.0 8 36.2299109918809 37.0 37.0 37.0 35.0 37.0 9 37.94805194805195 39.0 38.0 39.0 35.0 39.0 10-11 37.887776040648816 39.0 38.0 39.0 35.0 39.0 12-13 37.802260439059346 39.0 38.0 39.0 35.0 39.0 14-15 38.90584563635179 40.0 38.0 41.0 35.5 41.0 16-17 38.908728184719976 40.0 38.0 41.0 35.5 41.0 18-19 39.06156394904685 40.0 38.5 41.0 36.0 41.0 20-21 39.060176713391485 40.0 38.5 41.0 35.5 41.0 22-23 38.9310512788625 40.0 38.0 41.0 35.0 41.0 24-25 38.79314495354167 40.0 38.0 41.0 35.0 41.0 26-27 38.53733396106857 40.0 38.0 41.0 34.5 41.0 28-29 38.44830067333487 40.0 38.0 41.0 34.5 41.0 30-31 38.36467419948952 40.0 37.5 41.0 35.0 41.0 32-33 38.12066581389426 40.0 36.5 41.0 34.5 41.0 34-35 37.933323858084464 40.0 36.0 41.0 34.0 41.0 36-37 37.72012187538863 40.0 35.5 41.0 33.5 41.0 38-39 37.52295379039565 40.0 35.0 41.0 33.0 41.0 40-41 37.271183103262445 40.0 35.0 41.0 33.0 41.0 42-43 36.93713764575598 40.0 35.0 41.0 33.0 41.0 44-45 36.42052931109019 40.0 35.0 41.0 31.5 41.0 46-47 35.71953115284168 39.0 35.0 41.0 29.0 41.0 48-49 35.09897045498961 39.0 35.0 41.0 24.5 41.0 50-51 34.77444170057029 39.0 35.0 41.0 22.5 41.0 52-53 34.480053120614 39.0 35.0 41.0 20.5 41.0 54-55 34.270361421524214 38.5 35.0 40.0 19.5 41.0 56-57 34.027889802855604 38.0 35.0 40.0 15.5 41.0 58-59 33.786695862277256 38.0 34.0 40.0 10.0 41.0 60-61 33.446959333416245 38.0 34.0 40.0 6.5 41.0 62-63 33.05207389509715 37.0 33.5 40.0 2.0 41.0 64-65 32.68075221631993 36.5 33.0 40.0 2.0 41.0 66-67 32.2724074830778 36.0 33.0 39.0 2.0 41.0 68-69 31.838235294117645 35.5 32.5 39.0 2.0 40.0 70-71 31.437538863325457 35.0 32.0 38.5 2.0 40.0 72-73 30.9695844511166 35.0 31.5 37.5 2.0 40.0 74-75 30.505630370541454 35.0 31.0 37.0 2.0 39.0 76-77 29.211727397089916 33.5 29.5 35.5 2.0 38.5 78-79 29.696718010671496 34.5 31.0 36.0 2.0 38.5 80-81 29.476508785332314 35.0 31.0 36.0 2.0 37.5 82-83 29.123529115663178 34.5 30.0 35.0 2.0 37.0 84-85 28.768592214898646 34.0 30.0 35.0 2.0 37.0 86-87 28.413597574336286 34.0 29.0 35.0 2.0 36.0 88-89 28.0153499031748 34.0 29.0 35.0 2.0 36.0 90-91 27.567568888020325 34.0 28.0 35.0 2.0 35.5 92-93 27.177493619012086 34.0 27.0 35.0 2.0 35.0 94-95 26.79806616092526 34.0 26.0 35.0 2.0 35.0 96-97 26.392417432089115 33.5 25.0 35.0 2.0 35.0 98-99 25.828286282800647 33.0 23.5 35.0 2.0 35.0 100-101 24.44717844854644 31.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.003765347897392246 1101 2 0.24204878903803717 1101 3 -0.02373169542013187 1101 4 -5.584953528909864E-4 1101 5 -0.017126949595095198 1101 6 0.031359650929971394 1101 7 0.0582830699436272 1101 8 -0.1411856526269446 1101 9 0.02261613754773606 1101 10-11 0.004751230089780734 1101 12-13 -0.027039743959811346 1101 14-15 0.10488739856384655 1101 16-17 0.13613444831995736 1101 18-19 0.060738477132204594 1101 20-21 0.014656928908550526 1101 22-23 -0.13831031965269602 1101 24-25 0.14066675617844737 1101 26-27 -0.13420404018511078 1101 28-29 -0.16799786349005785 1101 30-31 0.13785546886972355 1101 32-33 0.19448200770223423 1101 34-35 0.2386714953530955 1101 36-37 0.1068743057652739 1101 38-39 0.04200399580959413 1101 40-41 0.081116028032163 1101 42-43 0.3167542926600504 1101 44-45 0.4654033432805349 1101 46-47 0.8109646564614934 1101 48-49 0.7532362750761834 1101 50-51 0.6890642385335397 1101 52-53 0.6757049060483311 1101 54-55 0.4742159216846318 1101 56-57 0.6873410814722192 1101 58-59 0.705783160236912 1101 60-61 0.8499727041899163 1101 62-63 0.7575113091148182 1101 64-65 0.965331998844043 1101 66-67 1.1047041888241402 1101 68-69 1.0356963601128122 1101 70-71 0.9002033771547602 1101 72-73 1.0383583862427201 1101 74-75 0.8128051537834331 1101 76-77 0.8985603827198894 1101 78-79 1.0267808852510676 1101 80-81 0.9120003512168289 1101 82-83 0.8064031696628824 1101 84-85 0.9071914665685057 1101 86-87 0.7901442264991161 1101 88-89 1.0306908541887339 1101 90-91 1.249984631075531 1101 92-93 1.0578994500299075 1101 94-95 0.6936965341691526 1101 96-97 0.6551328443115025 1101 98-99 0.31183964724547764 1101 100-101 0.3344743325513235 1102 1 0.10064204728253401 1102 2 0.07270247012090181 1102 3 -0.013002081686224187 1102 4 -0.04613459307491041 1102 5 -0.10567102354689695 1102 6 0.012170149444330036 1102 7 0.04500310956066045 1102 8 0.026584238426202944 1102 9 0.09287860040676321 1102 10-11 0.036420097702851706 1102 12-13 0.07202347822573074 1102 14-15 -0.04618556280954067 1102 16-17 -0.027941979544024775 1102 18-19 0.009958516749243529 1102 20-21 0.0963835713648109 1102 22-23 0.0906348767817704 1102 24-25 0.23055203184698314 1102 26-27 0.19737816516319384 1102 28-29 0.17086397209924797 1102 30-31 0.044652266492704484 1102 32-33 0.19312017211293409 1102 34-35 0.03607575418438813 1102 36-37 0.014228217947518829 1102 38-39 0.12735807306281544 1102 40-41 0.26608631525567006 1102 42-43 0.37665442471683974 1102 44-45 0.3962509827655225 1102 46-47 0.4629331279042148 1102 48-49 0.41860438336274086 1102 50-51 0.09952148514958736 1102 52-53 0.07570077927382357 1102 54-55 -0.06689087923976444 1102 56-57 -0.02035122622009311 1102 58-59 0.2632361150074658 1102 60-61 0.2406957150846054 1102 62-63 0.4105238545093428 1102 64-65 0.3099836990751399 1102 66-67 -0.023205187207555156 1102 68-69 -0.042898060488045786 1102 70-71 0.12096930650438154 1102 72-73 0.08586581443684693 1102 74-75 0.028944968903939383 1102 76-77 -0.1775786070457137 1102 78-79 0.005552757155985688 1102 80-81 0.30367252024950986 1102 82-83 0.3213503469491208 1102 84-85 0.42898083599736125 1102 86-87 0.435629535181846 1102 88-89 0.2858369420064939 1102 90-91 0.3659098539213552 1102 92-93 0.5330191264907853 1102 94-95 0.4708754711120342 1102 96-97 0.3717926330206467 1102 98-99 0.4218388218905744 1102 100-101 0.5547161615377867 1103 1 0.1429957857058426 1103 2 0.11935832161073279 1103 3 0.3303352902258041 1103 4 0.2588636614376867 1103 5 0.07153701801623669 1103 6 -0.04174487612192479 1103 7 0.05371001142319898 1103 8 0.13421920567265033 1103 9 0.149309181346041 1103 10-11 0.05150316236802155 1103 12-13 0.21000939111564065 1103 14-15 0.13670558038203495 1103 16-17 0.16701869565157068 1103 18-19 0.2698615908605859 1103 20-21 0.2675645476056445 1103 22-23 0.26458809771940395 1103 24-25 0.09057934359103825 1103 26-27 0.024965080168222187 1103 28-29 0.19310353511134792 1103 30-31 0.016282962428796566 1103 32-33 0.137387786134326 1103 34-35 0.46728301541197936 1103 36-37 0.2747524802224035 1103 38-39 0.06849921112059576 1103 40-41 0.16892427241935337 1103 42-43 0.08310964783120056 1103 44-45 0.22874066385470115 1103 46-47 0.3550620064798977 1103 48-49 0.3975866615318324 1103 50-51 0.061377451636225544 1103 52-53 -0.18613259855232656 1103 54-55 0.041693838504656355 1103 56-57 0.13634733837320567 1103 58-59 0.08168489008601654 1103 60-61 0.2588795867810134 1103 62-63 0.2785547393687793 1103 64-65 -0.015308413375322516 1103 66-67 0.18569885205564773 1103 68-69 0.05333990078266737 1103 70-71 0.0911676760137965 1103 72-73 0.31769221031338546 1103 74-75 0.38464449864909867 1103 76-77 0.17363399331981455 1103 78-79 0.5281978892996477 1103 80-81 0.8748730048789533 1103 82-83 0.8373472256069405 1103 84-85 0.917588963808111 1103 86-87 0.5784309397858252 1103 88-89 0.8399059811670178 1103 90-91 0.8012498132617658 1103 92-93 1.0340818202912239 1103 94-95 0.9497602073380875 1103 96-97 1.0223255200568104 1103 98-99 1.1264099453684082 1103 100-101 0.9156162169826807 1104 1 0.1705163944566337 1104 2 0.18166880757372184 1104 3 0.26662975036300196 1104 4 -0.06566507551971057 1104 5 -0.08394808027164657 1104 6 -0.05471541857883011 1104 7 -0.0721849560063248 1104 8 0.1943333464462711 1104 9 2.574721167789562E-4 1104 10-11 0.11084483527976374 1104 12-13 -0.020517501142386152 1104 14-15 0.07838137446746174 1104 16-17 0.13672527315777216 1104 18-19 0.08734876443526218 1104 20-21 0.12114441732374814 1104 22-23 0.08854324846593187 1104 24-25 -0.015554281819696314 1104 26-27 -0.28713499477269266 1104 28-29 -0.21226338712304482 1104 30-31 -0.3623646423443674 1104 32-33 -0.06411391444790127 1104 34-35 -0.2875449087247617 1104 36-37 -0.250563136310717 1104 38-39 -0.3704960041904073 1104 40-41 -0.3711777625146411 1104 42-43 -0.3384704524743327 1104 44-45 -0.4079400302134317 1104 46-47 -0.4004000330658428 1104 48-49 -0.2498221123195563 1104 50-51 -0.0920525362579312 1104 52-53 -0.13026126633349833 1104 54-55 -0.1084416316054444 1104 56-57 0.005552388237738626 1104 58-59 0.005389277011708771 1104 60-61 0.06572803319826193 1104 62-63 -0.045014177446809356 1104 64-65 0.05124488114246617 1104 66-67 -0.2541581837430549 1104 68-69 -0.014755447116105103 1104 70-71 0.11053701941611038 1104 72-73 0.14141677363845773 1104 74-75 0.27542100179084983 1104 76-77 0.23292090171895907 1104 78-79 0.23679015309165763 1104 80-81 0.4156379786496345 1104 82-83 0.4429879704043138 1104 84-85 0.06701537759723664 1104 86-87 0.013151640771589257 1104 88-89 -0.050576523012377805 1104 90-91 0.10356433450462887 1104 92-93 -0.1091417918607327 1104 94-95 -0.25827288195689135 1104 96-97 -0.5835256357626406 1104 98-99 -0.39598009325498396 1104 100-101 -0.02434170081290077 1105 1 -0.06476044698113981 1105 2 -0.14014809994555577 1105 3 -0.08882864832872173 1105 4 -0.04559365409996019 1105 5 -0.08635771882586596 1105 6 0.015671391820660574 1105 7 -0.05067175639346999 1105 8 -0.00428494945200697 1105 9 0.1651742027512242 1105 10-11 0.1343538297062281 1105 12-13 0.0930992929036023 1105 14-15 0.060843077234203236 1105 16-17 -0.07637087876293691 1105 18-19 -0.01992687552068162 1105 20-21 -0.09122417060135746 1105 22-23 0.07175253992539865 1105 24-25 0.16574932596323322 1105 26-27 0.10693435742835788 1105 28-29 -0.007223838346220646 1105 30-31 -0.3040393745140406 1105 32-33 -0.11286403960225755 1105 34-35 -0.19841831926442666 1105 36-37 -0.11475897784860933 1105 38-39 -0.1156483587610424 1105 40-41 -0.10311047953229746 1105 42-43 0.07158862038219382 1105 44-45 -0.09460358192721685 1105 46-47 -0.5239357270884781 1105 48-49 -0.7140980526459586 1105 50-51 -0.753845414140649 1105 52-53 -0.5793584862380499 1105 54-55 -0.6710989089141322 1105 56-57 -0.5667501181868531 1105 58-59 -0.5487608414677538 1105 60-61 -0.8172976985375442 1105 62-63 -0.8027220171157339 1105 64-65 -0.575494764420398 1105 66-67 -0.5422034395585591 1105 68-69 -0.3287426980589352 1105 70-71 -0.3334105158774143 1105 72-73 -0.245830831741511 1105 74-75 -0.4483950379915491 1105 76-77 -0.6020522318123973 1105 78-79 -0.5736026647169723 1105 80-81 -0.47434633533761783 1105 82-83 -0.6114691934310841 1105 84-85 -0.5000640203268709 1105 86-87 -0.6382651065501079 1105 88-89 -0.687668936989617 1105 90-91 -0.6980576659626117 1105 92-93 -0.6399297636926562 1105 94-95 -0.49439743557301696 1105 96-97 -0.36724889444835185 1105 98-99 -0.7227464384140134 1105 100-101 -0.7203067243419206 1106 1 -0.09288354677169508 1106 2 0.014984999169371349 1106 3 0.05858537775060313 1106 4 0.09916007875592925 1106 5 0.005105695808282462 1106 6 -0.05236455169666954 1106 7 0.015928473320734327 1106 8 0.03794192711038846 1106 9 -0.34670844031342796 1106 10-11 -0.20061931400027788 1106 12-13 -0.1590753912393552 1106 14-15 0.07836300831994691 1106 16-17 0.10405189672520976 1106 18-19 -0.01454294875897233 1106 20-21 -0.436492226813769 1106 22-23 0.12508086233604843 1106 24-25 -0.10412686483468292 1106 26-27 0.17391040634397115 1106 28-29 0.33847493200713075 1106 30-31 0.37401869588660475 1106 32-33 0.3684970170831008 1106 34-35 0.4501865047339564 1106 36-37 0.6873152063281296 1106 38-39 0.6615161909315432 1106 40-41 0.7824293488576757 1106 42-43 0.550572303917086 1106 44-45 0.6474155384024911 1106 46-47 0.3931644688442333 1106 48-49 0.884662166258174 1106 50-51 0.9174648013853286 1106 52-53 1.0367274201196253 1106 54-55 0.8780151712155941 1106 56-57 1.168673164023808 1106 58-59 1.0668056743082062 1106 60-61 0.9761453120698462 1106 62-63 0.8747955492649169 1106 64-65 0.657217552314961 1106 66-67 0.8237473407941245 1106 68-69 1.0158792869420843 1106 70-71 1.0946686679615105 1106 72-73 1.0977861535973155 1106 74-75 1.0635087185113896 1106 76-77 1.1862010956654778 1106 78-79 1.393971316770763 1106 80-81 1.4816532592843679 1106 82-83 1.3685279351413122 1106 84-85 1.25557326769421 1106 86-87 1.6661526692758528 1106 88-89 1.4587865389917347 1106 90-91 0.9287369762912938 1106 92-93 0.9345880053770017 1106 94-95 0.24850422647684312 1106 96-97 0.07157280678804767 1106 98-99 0.06008073542558634 1106 100-101 -0.18949377251434285 1107 1 0.11625691280010386 1107 2 0.24250637004224274 1107 3 0.20938727564096382 1107 4 0.11449220956919959 1107 5 0.06476768310339764 1107 6 0.08691864177835384 1107 7 0.10154920894931507 1107 8 0.08531076092734224 1107 9 -0.056093978702577374 1107 10-11 0.03924912769056732 1107 12-13 0.055618554333534576 1107 14-15 0.2836655336120799 1107 16-17 0.18290355541864045 1107 18-19 0.12357857112182558 1107 20-21 0.31977688933397985 1107 22-23 0.31520810725800885 1107 24-25 0.4444232488818898 1107 26-27 0.5723350397060614 1107 28-29 0.3240593959707425 1107 30-31 0.35138811789272495 1107 32-33 0.5069665488845203 1107 34-35 0.28761102045820763 1107 36-37 0.36624234929730903 1107 38-39 0.1938126774816169 1107 40-41 0.24638315640218167 1107 42-43 0.24306210863829136 1107 44-45 -0.04275582070659567 1107 46-47 0.05142656593465489 1107 48-49 0.2623384269436073 1107 50-51 0.08435857442272976 1107 52-53 0.18092689467064105 1107 54-55 -0.061484671933968116 1107 56-57 -0.11279495612362922 1107 58-59 -0.016285847925338715 1107 60-61 -0.2270740432748326 1107 62-63 -0.058077362663567556 1107 64-65 -0.3000897177030808 1107 66-67 -0.23589994177195095 1107 68-69 -0.3997939935849395 1107 70-71 -0.8020845278662669 1107 72-73 -0.8077212575861203 1107 74-75 -0.6759872033770868 1107 76-77 -0.4334022687765291 1107 78-79 -0.42513600814492847 1107 80-81 -0.559029824395342 1107 82-83 -0.35989042420305495 1107 84-85 -0.26860620842530736 1107 86-87 -0.18011919175465607 1107 88-89 -0.38450516361982423 1107 90-91 -0.5039202662779196 1107 92-93 -0.16725204638364488 1107 94-95 0.038496187891635 1107 96-97 0.3183377663990612 1107 98-99 0.14706410927862024 1107 100-101 0.3804272140749454 1108 1 0.062282821185917214 1108 2 0.08121074529264405 1108 3 0.11844082854153015 1108 4 0.15413553266382252 1108 5 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-1.395076815342172 2316 50-51 -1.7755443984435146 2316 52-53 -1.6196785847298898 2316 54-55 -1.5901509249190582 2316 56-57 -0.9867254952259472 2316 58-59 -1.024907348085172 2316 60-61 -0.8551555009382881 2316 62-63 -1.0692655689778476 2316 64-65 -0.7393913479845686 2316 66-67 -0.5814462835105481 2316 68-69 -0.7548738153257837 2316 70-71 -0.8894277519500555 2316 72-73 -0.9028022075494526 2316 74-75 -0.9154618277730187 2316 76-77 -1.1154409668077783 2316 78-79 -1.2393459703494756 2316 80-81 -1.1239308228722429 2316 82-83 -1.4020201321814696 2316 84-85 -1.2325971074305961 2316 86-87 -1.7450671995017828 2316 88-89 -1.5234645664140558 2316 90-91 -1.486761882644668 2316 92-93 -1.1796342881617257 2316 94-95 -1.257709223815553 2316 96-97 -1.773527749480671 2316 98-99 -2.4371200918458413 2316 100-101 -2.3668133417626684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 10.0 6 15.0 7 38.0 8 73.0 9 149.0 10 305.0 11 573.0 12 1019.0 13 1588.0 14 1914.0 15 2994.0 16 4642.0 17 7778.0 18 6836.0 19 3249.0 20 2231.0 21 2335.0 22 2301.0 23 2370.0 24 2568.0 25 2774.0 26 3133.0 27 3612.0 28 4237.0 29 4994.0 30 6093.0 31 7516.0 32 9655.0 33 12809.0 34 18398.0 35 29686.0 36 43918.0 37 71240.0 38 68611.0 39 8053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.08558529200908 20.31176864420337 16.82525669662695 17.777389367160605 2 15.651334529583504 23.47404077910234 40.01575260125192 20.858872090062242 3 18.549280177187157 29.694541664445907 32.069275913325164 19.68690224504178 4 11.719698450204605 19.564316212743023 38.82157514168458 29.894410195367787 5 11.320419459062466 40.32718043787925 33.47558140567946 14.876818697378823 6 27.052723837949554 39.86414861927858 17.262719041104816 15.820408501667051 7 24.627060126376133 37.91106294526267 21.077098915676206 16.38477801268499 8 26.60087290730246 33.977946358247316 19.60606652809115 19.815114206359077 9 28.225285886024597 17.026133920798763 20.682691681323693 34.06588851185294 10-11 23.625348659548386 29.23721285554391 26.151982991928275 20.985455492979433 12-13 26.89280426413977 27.58010068107788 27.45158424637252 18.07551080840983 14-15 23.94972788269642 27.30855555752957 28.83658748911827 19.905129070655747 16-17 18.770616068837683 31.077779949188976 29.546490900800066 20.605113081173272 18-19 18.45452768845382 27.509016291506033 35.78742279152676 18.249033228513394 20-21 20.25230811140524 26.421583432527346 36.07123610543583 17.254872350631583 22-23 19.7721434652256 26.37524521597513 35.81448717474183 18.038124144057445 24-25 19.39618797765019 26.658395539513386 36.302450839598364 17.642965643238057 26-27 19.224488285153793 26.684235851500908 36.33800940148795 17.753266461857347 28-29 18.658825654441735 26.425810195980887 37.34341294270405 17.571951206873322 30-31 18.6276062910755 27.399312737162678 36.58453170040552 17.3885492713563 32-33 18.37931790052173 27.99832406535553 35.761217806361444 17.861140227761293 34-35 18.71198796643393 26.885870627320696 36.327956129597716 18.074185276647654 36-37 19.173963123406597 27.26895869667566 35.55123904050977 18.00583913940797 38-39 19.044890671428 27.28883795919999 36.04060162175527 17.625669747616744 40-41 18.579038380679968 27.708144568609683 36.02489621641469 17.687920834295664 42-43 18.585550182273696 28.168023477918382 35.08094965929181 18.165476680516115 44-45 18.95923274172248 28.247789602098766 34.300400921467954 18.4925767347108 46-47 19.04969175831009 28.71488976140139 33.28003505842083 18.955383421867687 48-49 19.48836617099271 29.0721362540788 32.58123545401247 18.85826212091602 50-51 20.098809225945047 29.477246784702782 31.675803879824056 18.748140109528116 52-53 19.485701257247083 29.770343655432573 31.69352307519202 19.05043201212832 54-55 19.008089493725606 29.85872996132914 31.74267592872244 19.390504616222813 56-57 19.675946488531988 30.33841443554166 30.778865457388026 19.206773618538325 58-59 19.512350394703336 29.310201882021307 31.50357394543441 19.673873777840946 60-61 19.609915847945945 29.68565861862715 31.627788536133266 19.076636997293633 62-63 20.289616903843992 29.403473863118183 31.744600588649835 18.562308644387986 64-65 20.03304493044575 29.16925755503047 32.0749018423437 18.722795672180077 66-67 19.622056010564904 29.848218357110284 31.69396722748296 18.83575840484185 68-69 19.652702518639593 29.9597005821356 31.426291446811284 18.961305452413523 70-71 19.62531312736511 30.188735113495714 31.12204712751908 19.06390463162009 72-73 19.64396752358449 29.63532135898757 30.762727924150635 19.95798319327731 74-75 19.465862454918543 30.155867843966337 30.656427475852922 19.721842225262197 76-77 19.657588193839903 29.35743007562433 31.553911205073998 19.43107052546177 78-79 19.30048975192614 28.983601897418588 31.802340386471712 19.91356796418356 80-81 19.171241435263322 30.68662983163667 30.73445022829428 19.407678504805727 82-83 19.121785979192794 30.24358972460874 30.35033511538422 20.284289180814255 84-85 18.96444259545217 29.318980284050618 30.560831928082756 21.15574519241446 86-87 19.306707883999266 29.76027620350466 30.394821776490723 20.53819413600535 88-89 19.493103795429377 28.70511841100076 30.85644405753845 20.94533373603141 90-91 18.989753916313777 28.668897509550177 31.260919778495072 21.080428795640973 92-93 19.141816954311867 29.02001252540985 30.939835096909068 20.898335423369215 94-95 18.728717702725913 28.69993663427316 30.959339338272308 21.612006324728622 96-97 19.065977342311133 28.878337804466398 30.02972859334008 22.02595625988239 98-99 19.19283892163351 29.216690429341945 29.90884312044641 21.681627528578133 100-101 18.86509154016099 29.817186213595683 30.14132308257272 21.17639916367061 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1440.0 1 966.0 2 604.5 3 694.0 4 579.0 5 583.0 6 967.5 7 2013.0 8 3060.5 9 3386.0 10 2848.0 11 2091.5 12 1966.5 13 1999.0 14 1931.5 15 1860.5 16 1866.0 17 1816.5 18 1743.0 19 1754.5 20 1778.0 21 1857.5 22 1964.0 23 2168.5 24 2341.5 25 2525.0 26 2818.5 27 3151.0 28 3439.0 29 3914.5 30 4759.0 31 5390.5 32 6344.0 33 7628.0 34 8525.5 35 9195.0 36 10727.0 37 11860.5 38 11874.5 39 11614.0 40 11173.5 41 10855.0 42 10197.0 43 10222.0 44 10836.5 45 10964.0 46 10472.0 47 10560.5 48 14717.0 49 14337.0 50 9909.5 51 8427.5 52 6982.5 53 6435.5 54 6101.5 55 5630.0 56 5143.5 57 4730.5 58 4349.0 59 3951.5 60 3466.0 61 2864.5 62 2214.0 63 1654.5 64 1124.0 65 801.5 66 638.5 67 494.5 68 339.0 69 184.0 70 116.0 71 109.0 72 93.5 73 87.0 74 73.0 75 52.0 76 39.0 77 23.0 78 15.5 79 7.5 80 3.0 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04856065047583516 2 0.0 3 0.0 4 0.0 5 0.01450897483729221 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00651423360041691 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0028129645092709386 24-25 2.9610152729167777E-4 26-27 0.0028129645092709386 28-29 0.0031090660365626164 30-31 0.0016285584001042275 32-33 0.0 34-35 0.0 36-37 2.9610152729167777E-4 38-39 4.4415229093751666E-4 40-41 0.0 42-43 0.012732365673542143 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.4805076364583889E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.402538182291944E-4 84-85 1.4805076364583889E-4 86-87 0.0 88-89 0.0 90-91 0.009475248873333689 92-93 0.002516862981979261 94-95 0.0 96-97 0.0 98-99 0.0022207614546875835 100-101 0.016581685528333955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 337722.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.07499089068245 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49021061648428 37.25341169504547 2 11.98276069858537 11.521422228594389 3 4.010041125380272 5.783480717217558 4 1.8960797547501727 3.646160677504879 5 1.0752058862979443 2.5845256594690915 6 0.663221349797499 1.9130616210012532 7 0.46597507555723516 1.5681223258889405 8 0.3370932351960298 1.2964603369087848 9 0.25800180877053414 1.116309114578072 >10 1.631782257071305 15.255900733985023 >50 0.1171232951263852 3.9983937972765586 >100 0.06382909734400885 5.718885875308906 >500 0.004337899819495747 1.4964557070054507 >1k 0.003098499871068391 3.158853247338004 >5k 0.0012393999484273566 3.68855626287766 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6743 1.9966125985277832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5638 1.6694204108704793 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4231 1.2528055619710887 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2362 0.6993918074629428 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1567 0.4639910932660591 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1274 0.3772333457695975 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1169 0.3461426854039713 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 980 0.2901794967458442 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 924 0.27359781121751026 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 915 0.27093289747188515 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 629 0.18624786066646531 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 536 0.1587104186283393 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 522 0.1545649972462558 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 517 0.1530844896097974 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 487 0.14420144379104707 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 461 0.13650280408146345 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 452 0.13383789033583834 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 426 0.12613925062625472 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 412 0.12199382924417124 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 410 0.1214016261895879 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 395 0.11696010328021272 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 377 0.11163027578896252 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 357 0.10570824524312897 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.4805076364583889E-4 0.0 18-19 0.0 0.0 0.0 2.9610152729167777E-4 0.0 20-21 0.0 0.0 0.0 2.9610152729167777E-4 0.0 22-23 0.0 0.0 0.0 8.883045818750333E-4 0.0 24-25 0.0 0.0 0.0 0.0026649137456251 0.0 26-27 0.0 0.0 0.0 0.0042934721457293275 0.0 28-29 0.0 0.0 0.0 0.00932719810968785 0.0 30-31 0.0 0.0 0.0 0.018358294692084023 0.0 32-33 0.0 0.0 0.0 0.02812964509270939 0.0 34-35 0.0 0.0 0.0 0.04056590923895985 0.0 36-37 0.0 0.0 0.0 0.05359437643979367 0.0 38-39 0.0 0.0 0.0 0.07106436655000267 0.0 40-41 0.0 0.0 0.0 0.09119927040583675 0.0 42-43 0.0 0.0 0.0 0.10881731127969158 0.0 44-45 0.0 0.0 0.0 0.12391848917156714 0.0 46-47 0.0 0.0 0.0 0.1431650884455262 0.0 48-49 0.0 0.0 0.0 0.15856236786469347 0.0 50-51 0.0 0.0 0.0 0.1763284595021941 0.0 52-53 0.0 0.0 0.0 0.21304504888636216 0.0 54-55 0.0 0.0 0.0 0.265603069980635 0.0 56-57 0.0 0.0 0.0 0.3683502999508471 0.0 58-59 0.0 0.0 0.0 0.5011518349411646 0.0 60-61 0.0 0.0 0.0 0.6841425788074215 0.0 62-63 0.0 0.0 0.0 0.782004133577321 0.0 64-65 0.0 0.0 0.0 0.8983720338029504 0.0 66-67 0.0 0.0 0.0 1.1123053872711877 0.0 68-69 0.0 0.0 0.0 1.3888642137616145 0.0 70-71 0.0 0.0 0.0 1.7360432545111069 0.0 72-73 0.0 0.0 0.0 2.1499931896648725 0.0 74-75 0.0 0.0 0.0 2.5372939873623865 0.0 76-77 0.0 0.0 0.0 2.8810678605480247 0.0 78-79 0.0 0.0 0.0 3.1770213370760567 0.0 80-81 0.0 0.0 0.0 3.3942118073445022 0.0 82-83 0.0 0.0 0.0 3.7590088889678492 0.0 84-85 0.0 0.0 0.0 4.468912300649647 0.0 86-87 0.0 0.0 0.0 5.338266384778013 0.0 88-89 0.0 0.0 0.0 6.223017748325546 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 85 3.6280835E-7 39.121002 4 GGTAAGG 70 2.0492412E-4 33.93148 3 ACGCCGA 35 2.0531911E-4 33.926456 78-79 GTATAGG 160 5.2750693E-11 32.683247 1 CCGCTCG 30 0.0039481507 31.669384 86-87 TATAGGG 75 3.0723333E-4 31.669382 2 GGGACGC 30 0.003951014 31.664696 76-77 CCGACCG 30 0.003951014 31.664696 82-83 GACGCCG 30 0.003951014 31.664696 78-79 GGTGTGC 195 0.0 31.664692 8 GTAGATA 105 1.8973406E-6 31.664692 6 ATATAGG 170 1.07320375E-10 30.73793 2 GGTACTC 95 3.4815843E-5 30.002573 3 GGACTGA 95 3.4851066E-5 29.998129 6 AGGTAAG 80 4.484743E-4 29.690044 2 GTTATAC 70 0.008349594 27.165297 1 CACTTAG 70 0.008349594 27.165297 1 GACCGCT 35 0.008385656 27.145184 84-85 CGATTGC 35 0.008388686 27.143173 92-93 AGCCCGT 35 0.0083917165 27.141165 44-45 >>END_MODULE