FastQCFastQC Report
Thu 26 May 2016
SRR937329_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937329_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415747
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65261.569704652108133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50281.2093893642046727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36370.8748108825800306No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19050.45821136412289204No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16650.4004839481704017No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14510.34901033561276446No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11970.28791548706304554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.24702523409669822No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.23764452900441854No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8310.19988117773549777No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7190.17294171695766897No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6960.16740950626222198No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6340.15249659047449532No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6160.14816703427805852No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6000.14431853988122584No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.13998898368478907No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.12194916619963583No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4650.11184686840795004No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG4210.10126350881666014No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC200.002062826471.339641
GTGGTAC1152.4647306E-937.2206761
CTCCCGC1106.99838E-834.543097
GTCTAGC702.0359848E-433.9712561
TCCCGCT1252.1495907E-730.3979198
TACCTGG2350.030.3463132
CTAGAAC650.005814324629.2569583
ATGGGAT10600.028.246215
CCCGCTG1354.216672E-728.1462239
GTACATG40000.028.0602571
GGTACTC1405.745933E-727.1671753
CATGGGT3500.027.1671734
TACATGG41200.027.117722
GTACCTG3050.026.5087171
AGTACTC908.9100556E-426.4029835
GGTATCA48350.026.3619731
ACATGGG39400.025.5812763
TATGGTG950.001218853425.0223982
GAGTACT38800.024.85010512-13
GTATCAA65350.024.7441731
Produced by FastQC (version 0.11.5)