##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937329_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 415747 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.879626311194066 33.0 31.0 34.0 30.0 34.0 2 31.953055584285636 33.0 31.0 34.0 30.0 34.0 3 31.960064654705867 33.0 31.0 34.0 30.0 34.0 4 35.620329190589466 37.0 35.0 37.0 33.0 37.0 5 35.6167717385814 37.0 35.0 37.0 35.0 37.0 6 35.72281459637713 37.0 35.0 37.0 35.0 37.0 7 35.675706619650896 37.0 35.0 37.0 33.0 37.0 8 35.680981462283555 37.0 35.0 37.0 35.0 37.0 9 37.297260112520355 39.0 37.0 39.0 34.0 39.0 10-11 37.23022054278203 39.0 37.0 39.0 34.0 39.0 12-13 37.1304904184516 39.0 37.0 39.0 34.0 39.0 14-15 38.21004841887013 40.0 38.0 41.0 33.5 41.0 16-17 38.185876266094525 40.0 38.0 41.0 33.5 41.0 18-19 38.26569043192134 40.0 38.0 41.0 34.0 41.0 20-21 38.260733090076414 40.0 38.0 41.0 34.0 41.0 22-23 38.129262508208114 40.0 38.0 41.0 34.0 41.0 24-25 37.988296969070134 40.0 38.0 41.0 33.0 41.0 26-27 37.78956793434469 40.0 37.0 41.0 33.0 41.0 28-29 37.60190813162813 40.0 37.0 41.0 33.0 41.0 30-31 37.45545488001116 39.0 37.0 41.0 33.0 41.0 32-33 37.19518240660787 39.0 35.5 41.0 33.0 41.0 34-35 36.94815837516566 39.0 35.0 41.0 32.0 41.0 36-37 36.8594132970292 39.0 35.0 41.0 32.0 41.0 38-39 36.77740067877819 39.0 35.0 41.0 31.5 41.0 40-41 36.553471221680496 39.0 35.0 41.0 31.0 41.0 42-43 36.24287006280262 39.0 35.0 41.0 30.5 41.0 44-45 35.87319571758786 39.0 35.0 41.0 30.0 41.0 46-47 35.322485790637096 39.0 35.0 41.0 26.5 41.0 48-49 34.85848123979247 39.0 35.0 40.0 24.0 41.0 50-51 33.887381027403684 38.0 34.0 39.5 22.5 40.5 52-53 33.97575328264546 38.0 34.0 39.5 22.0 40.5 54-55 34.31164145501952 38.0 35.0 40.0 22.0 41.0 56-57 34.109388642611975 38.0 34.5 40.0 20.5 41.0 58-59 33.882270948437395 38.0 34.0 40.0 18.5 41.0 60-61 33.60394061773146 38.0 34.0 40.0 16.5 41.0 62-63 33.261816681780026 37.0 33.5 40.0 11.5 41.0 64-65 32.870919092621236 37.0 33.0 40.0 7.5 41.0 66-67 32.468551787505376 36.0 33.0 39.0 4.0 41.0 68-69 31.989037804241523 35.5 32.0 39.0 2.0 40.5 70-71 31.537754932687427 35.0 31.5 38.5 2.0 40.0 72-73 31.09778422935102 35.0 31.0 38.0 2.0 40.0 74-75 30.616869153595815 35.0 31.0 37.0 2.0 39.5 76-77 30.159742583831033 35.0 30.5 37.0 2.0 39.0 78-79 29.689783690561807 35.0 30.0 36.0 2.0 39.0 80-81 29.232052185584024 34.0 29.0 36.0 2.0 37.5 82-83 28.78996120236586 34.0 29.0 35.0 2.0 37.0 84-85 28.32265777023045 34.0 29.0 35.0 2.0 36.5 86-87 27.866465663011397 34.0 27.5 35.0 2.0 36.0 88-89 27.467100183525076 34.0 26.5 35.0 2.0 36.0 90-91 26.996009592372282 34.0 25.5 35.0 2.0 35.5 92-93 26.569084082386645 33.0 25.0 35.0 2.0 35.0 94-95 26.049190974318513 33.0 24.0 35.0 2.0 35.0 96-97 25.4287980430406 33.0 19.5 35.0 2.0 35.0 98-99 24.759493153287938 32.0 10.0 35.0 2.0 35.0 100-101 23.073372748330115 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16514613917220444 1101 2 0.1178501368525886 1101 3 0.08157877611814612 1101 4 -0.2515130015993634 1101 5 0.11087831158172179 1101 6 0.10295891642978461 1101 7 0.10096346547258861 1101 8 0.027596861120294136 1101 9 0.05794246374409795 1101 10-11 0.06555292876097951 1101 12-13 0.10607849645512601 1101 14-15 0.3242352560666504 1101 16-17 0.3441108688914838 1101 18-19 0.2417559095379218 1101 20-21 0.5052627657361768 1101 22-23 0.5299411652471733 1101 24-25 0.378903353621439 1101 26-27 0.28247863424556385 1101 28-29 0.40882041359778754 1101 30-31 0.20283270756391403 1101 32-33 0.530834788824194 1101 34-35 0.40910652835795247 1101 36-37 0.4075730712422896 1101 38-39 0.382569448625695 1101 40-41 0.26142816573035077 1101 42-43 0.09233196884797934 1101 44-45 -0.2552060757889265 1101 46-47 -0.11673426328689374 1101 48-49 -0.006464466404281666 1101 50-51 0.03591290653926649 1101 52-53 0.14855617414270483 1101 54-55 0.2354823787330531 1101 56-57 0.356383738203057 1101 58-59 0.2289002635942694 1101 60-61 0.016305549939865216 1101 62-63 0.3263061033418282 1101 64-65 0.15128130600896839 1101 66-67 0.17148276995434486 1101 68-69 0.26776884884085916 1101 70-71 0.5700948372818821 1101 72-73 0.5269149027693096 1101 74-75 0.7328300742938829 1101 76-77 0.4847740176420814 1101 78-79 0.6861887386743213 1101 80-81 0.6388009880566088 1101 82-83 0.7650041641925753 1101 84-85 0.7070964977408885 1101 86-87 0.719797343932612 1101 88-89 1.0072468272514463 1101 90-91 1.4675192772545564 1101 92-93 1.4424207742716781 1101 94-95 1.2818611301244403 1101 96-97 1.4687785034896876 1101 98-99 1.4647505319709353 1101 100-101 1.8002044858129231 1102 1 0.01920590265895683 1102 2 -0.19571547523428023 1102 3 0.042925916762886374 1102 4 -0.3751222194784489 1102 5 -0.3379330558987803 1102 6 -0.1310778665903598 1102 7 -0.23156816835295047 1102 8 -0.16548544748881255 1102 9 -0.35146311294428756 1102 10-11 -0.2569979030319942 1102 12-13 -0.030259187941709342 1102 14-15 0.0421321865328963 1102 16-17 -0.01634167501762107 1102 18-19 0.0362984055591653 1102 20-21 -0.016438431103658502 1102 22-23 0.12653941387869594 1102 24-25 -0.06258209803037573 1102 26-27 -0.2719911378767108 1102 28-29 -0.1931696805364851 1102 30-31 -0.09925694480300251 1102 32-33 -0.09995582486845223 1102 34-35 -0.05036720468325484 1102 36-37 0.13337042920230857 1102 38-39 0.249154280090373 1102 40-41 -0.10550101589383587 1102 42-43 -0.11504354767726 1102 44-45 0.19852432479914484 1102 46-47 0.23461530386673957 1102 48-49 0.20709507941034389 1102 50-51 -0.08058449923725419 1102 52-53 -0.003601991416573469 1102 54-55 -0.07589759114969041 1102 56-57 -0.12083027274478297 1102 58-59 -0.3364656039897156 1102 60-61 -0.23831016955570306 1102 62-63 0.04786956011786003 1102 64-65 0.18111309184814672 1102 66-67 0.24302017744368953 1102 68-69 0.35449869989603044 1102 70-71 0.3415230518709116 1102 72-73 0.5160188073075993 1102 74-75 0.30892075435868804 1102 76-77 0.19771266265096799 1102 78-79 0.24818960936280732 1102 80-81 0.3975703198388807 1102 82-83 0.18757176744409776 1102 84-85 0.07836127981898144 1102 86-87 0.14558720826221716 1102 88-89 0.1874775597002909 1102 90-91 -0.06107427536857202 1102 92-93 0.02770364097783684 1102 94-95 0.0764464468908983 1102 96-97 0.12867651874814356 1102 98-99 -0.08275016386617651 1102 100-101 -0.2353920428519949 1103 1 -0.1375186790656251 1103 2 -0.17970839672720373 1103 3 -0.22551842043145243 1103 4 -0.2290314858877167 1103 5 -0.31317436864009096 1103 6 -0.3065604920729825 1103 7 -0.14127698431176583 1103 8 -0.23137186962523515 1103 9 -0.02189425837543979 1103 10-11 -0.08512290303199421 1103 12-13 -0.09618582886834304 1103 14-15 -0.03105394602823708 1103 16-17 -0.15852314219907981 1103 18-19 -0.15267762404187124 1103 20-21 -0.06957549674071828 1103 22-23 -0.1172226517583681 1103 24-25 -0.1404858946841614 1103 26-27 -0.2417184544790274 1103 28-29 -0.3597197127115166 1103 30-31 -0.33434381738987184 1103 32-33 -0.24119617235880497 1103 34-35 -0.29673142604747227 1103 36-37 -0.04974170078482132 1103 38-39 -0.1399987121876194 1103 40-41 -0.13184432348715092 1103 42-43 -0.10220168796040241 1103 44-45 0.13908497473479287 1103 46-47 0.13253599241243563 1103 48-49 0.026737936553203667 1103 50-51 -0.1536781285808786 1103 52-53 0.06002413470955048 1103 54-55 0.396443946816845 1103 56-57 0.520739868825359 1103 58-59 0.44502973063061546 1103 60-61 0.24720704408651528 1103 62-63 0.26959977247307876 1103 64-65 0.16406434667440095 1103 66-67 0.4753721722956854 1103 68-69 0.5406393047866409 1103 70-71 0.3947405550884149 1103 72-73 0.46099145853024837 1103 74-75 0.20527693196486396 1103 76-77 0.38173373729703997 1103 78-79 0.37605318722638614 1103 80-81 0.24632755922111826 1103 82-83 0.5667062590785861 1103 84-85 0.4205226376053375 1103 86-87 0.636292645842655 1103 88-89 0.5098673602150932 1103 90-91 0.09555378125948621 1103 92-93 0.16096860236779875 1103 94-95 0.5518164597609143 1103 96-97 0.6989829859296108 1103 98-99 0.755518015027004 1103 100-101 0.7400342763243231 1104 1 -0.08378704720174213 1104 2 0.014655485716726702 1104 3 0.011222685523691212 1104 4 0.04292327049279265 1104 5 0.06626298634830619 1104 6 -0.11618926237461125 1104 7 0.049147416306951186 1104 8 -0.04199155253320441 1104 9 -0.026283276163525215 1104 10-11 -0.020338970317375527 1104 12-13 -0.12130183814909401 1104 14-15 -0.11440419738168117 1104 16-17 -0.14419599602738487 1104 18-19 0.026032256081876426 1104 20-21 -0.025810995580627605 1104 22-23 0.041760816919129695 1104 24-25 -0.07810673768493359 1104 26-27 0.023355501437450243 1104 28-29 0.05172411946172417 1104 30-31 0.11052277193727633 1104 32-33 0.07865591580824827 1104 34-35 0.24837684928122172 1104 36-37 0.15844903780759978 1104 38-39 -0.038947378080891326 1104 40-41 -0.26208890972070975 1104 42-43 -0.21143651007950837 1104 44-45 0.09343290590261688 1104 46-47 0.23077893518118486 1104 48-49 0.0532170548826727 1104 50-51 -0.29925527475258207 1104 52-53 -0.021520722212336807 1104 54-55 0.14091658796147044 1104 56-57 0.179479234641299 1104 58-59 0.07394746071569358 1104 60-61 0.16404047409424294 1104 62-63 0.28980713906239686 1104 64-65 0.35705303576953185 1104 66-67 0.28756281421370744 1104 68-69 0.06766463347959828 1104 70-71 0.083913022219118 1104 72-73 0.161247645691887 1104 74-75 0.379218347278087 1104 76-77 0.6159395570959596 1104 78-79 0.47609185698663126 1104 80-81 0.5754676404276147 1104 82-83 0.6701188654204273 1104 84-85 0.6285296948365833 1104 86-87 0.836261226662451 1104 88-89 0.6192761962553099 1104 90-91 1.0806272538039572 1104 92-93 0.6752884979594462 1104 94-95 0.6817371867524074 1104 96-97 0.3574589140038569 1104 98-99 0.6462203545474985 1104 100-101 1.2900874472446624 1105 1 0.0392512324388008 1105 2 0.17630192775657605 1105 3 0.17770798074849026 1105 4 0.09888149346331687 1105 5 0.19888315348380559 1105 6 0.11069614509515446 1105 7 0.17666180624870975 1105 8 0.35485600648095783 1105 9 0.371837305046391 1105 10-11 0.3993442798588589 1105 12-13 0.25064307968622757 1105 14-15 0.5142908179835572 1105 16-17 0.6809311350870573 1105 18-19 0.40345253820001403 1105 20-21 0.470284385265181 1105 22-23 0.4967430562062347 1105 24-25 0.7204101230149504 1105 26-27 0.9062443036330734 1105 28-29 0.7296700365510205 1105 30-31 0.4942328787753141 1105 32-33 0.5503414382606664 1105 34-35 0.5911004323596103 1105 36-37 0.5520558655868584 1105 38-39 0.6890296801427596 1105 40-41 0.4858732281352687 1105 42-43 0.6141423385882732 1105 44-45 0.5660115971536683 1105 46-47 0.7793556826779167 1105 48-49 0.6755948687065967 1105 50-51 0.6145556472303255 1105 52-53 0.34892531465055043 1105 54-55 0.819738563336017 1105 56-57 1.1158726121881912 1105 58-59 0.8388626952323861 1105 60-61 1.193621129632234 1105 62-63 1.3361834745379682 1105 64-65 1.0642579307451996 1105 66-67 1.222385446631968 1105 68-69 1.2411002192409164 1105 70-71 1.044593062463047 1105 72-73 1.1561795116275952 1105 74-75 1.2807747195754828 1105 76-77 1.1934501827247708 1105 78-79 1.4499469925361197 1105 80-81 1.3758906123184644 1105 82-83 1.1784687959517441 1105 84-85 1.4501594370153654 1105 86-87 1.0440821001199403 1105 88-89 1.1775203838139134 1105 90-91 1.4055832797845618 1105 92-93 1.1255242808338757 1105 94-95 1.2397589376370206 1105 96-97 1.3694568059886087 1105 98-99 0.7945205961479509 1105 100-101 1.0756721819290398 1106 1 0.3735206813279781 1106 2 0.09742874964425852 1106 3 0.12024572470999928 1106 4 0.14444637389087944 1106 5 0.20910422914588622 1106 6 0.20653913892332554 1106 7 -0.0020165292072107377 1106 8 -0.027351266919211525 1106 9 -0.04978478728072844 1106 10-11 0.30942276117465184 1106 12-13 0.10003188330877322 1106 14-15 -0.181449856237343 1106 16-17 0.04322962532859265 1106 18-19 -0.23623005331616298 1106 20-21 -0.1401935779215293 1106 22-23 -0.26658842666611093 1106 24-25 -0.1163084654098725 1106 26-27 -0.16331670171149426 1106 28-29 0.11260116674727527 1106 30-31 0.07981669921529289 1106 32-33 0.11620696417256227 1106 34-35 0.11695246079139565 1106 36-37 -0.28935962821410044 1106 38-39 -0.3630499114496146 1106 40-41 -0.4281619741636504 1106 42-43 -0.7162737974314979 1106 44-45 -0.7035080449454014 1106 46-47 -0.41293986859617604 1106 48-49 -0.7115923217493787 1106 50-51 -0.4608506378059758 1106 52-53 -0.6410060005918012 1106 54-55 -0.7356555304279269 1106 56-57 -0.5914987535608631 1106 58-59 -0.7589178400950871 1106 60-61 -0.5683333925678582 1106 62-63 -0.659516924943901 1106 64-65 -1.1341713544326097 1106 66-67 -0.9610823172492111 1106 68-69 -1.0965762548689568 1106 70-71 -1.1278916712338507 1106 72-73 -0.8785764996493342 1106 74-75 -0.8667941012454676 1106 76-77 -1.0028565148554804 1106 78-79 -1.1468865421709111 1106 80-81 -0.9294681049855242 1106 82-83 -0.507448107465077 1106 84-85 -0.7056112799838381 1106 86-87 -0.6699073849076527 1106 88-89 -0.8148981686901955 1106 90-91 -0.6950981252595803 1106 92-93 -0.21847097327795595 1106 94-95 -0.1019504529082127 1106 96-97 -0.395839431044557 1106 98-99 -0.40785452494839447 1106 100-101 -0.5302993645120857 1107 1 0.38730210946484434 1107 2 0.21433282191078717 1107 3 0.3078821172029542 1107 4 0.32621044959615375 1107 5 0.5285371008939634 1107 6 0.42981943624242547 1107 7 0.41483860708608944 1107 8 0.5503709619159807 1107 9 0.6482670319471424 1107 10-11 0.5118193012690782 1107 12-13 0.5671654256119467 1107 14-15 0.7715782224305414 1107 16-17 0.8148639545751095 1107 18-19 0.7092063365316079 1107 20-21 0.8596807756607134 1107 22-23 0.534916685159196 1107 24-25 0.6067139261043621 1107 26-27 0.7115857569905089 1107 28-29 0.8006409503709264 1107 30-31 0.5245719532194428 1107 32-33 0.18965060183474947 1107 34-35 0.4881252047768996 1107 36-37 0.45141197305029834 1107 38-39 0.5722884741923124 1107 40-41 0.46952216396805113 1107 42-43 0.5000496561256185 1107 44-45 0.8493560320824614 1107 46-47 0.7323007773586667 1107 48-49 1.049004961642808 1107 50-51 0.9441937352183984 1107 52-53 0.9567535253905604 1107 54-55 0.7514954701143353 1107 56-57 0.833463883162274 1107 58-59 0.9175095693402966 1107 60-61 0.6778410046599888 1107 62-63 0.8489344498924325 1107 64-65 0.8965128233769093 1107 66-67 0.9842879429050058 1107 68-69 0.7855541793386074 1107 70-71 0.6789699457694205 1107 72-73 0.6896428922220075 1107 74-75 0.6755950323232867 1107 76-77 0.4381293466160372 1107 78-79 0.7203184202012949 1107 80-81 0.7140134911207632 1107 82-83 0.22132095441909172 1107 84-85 0.5212730856340109 1107 86-87 0.5320923770254495 1107 88-89 0.3652325035842665 1107 90-91 0.3647294084996311 1107 92-93 0.5222477242315975 1107 94-95 0.5749795421981894 1107 96-97 0.586001355105239 1107 98-99 0.5862863842026336 1107 100-101 0.4699849931702005 1108 1 0.016235899688954447 1108 2 -0.07416810368690818 1108 3 -0.046099922262953896 1108 4 -0.004168848525083035 1108 5 0.05536958740386666 1108 6 0.10332961781712413 1108 7 -0.15375409053886813 1108 8 -0.07846436046772709 1108 9 0.02142075993958059 1108 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550.0 10 631.0 11 988.0 12 1514.0 13 2255.0 14 2663.0 15 3602.0 16 4988.0 17 7223.0 18 5815.0 19 3265.0 20 3003.0 21 3125.0 22 3312.0 23 3595.0 24 3955.0 25 4421.0 26 4916.0 27 5545.0 28 6520.0 29 7430.0 30 9187.0 31 11319.0 32 14604.0 33 18802.0 34 26494.0 35 40853.0 36 55883.0 37 77236.0 38 68661.0 39 10323.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.320729355949545 20.016506070324546 15.936321318168984 19.726443255556923 2 14.580720171359102 22.294050674437006 41.022944366183594 22.102284788020302 3 17.73576075707831 29.356082992997074 31.391346091218963 21.516810158705653 4 10.98435871704813 18.21664286541356 39.27592728395538 31.523071133582924 5 11.261806674449343 40.186350085384326 33.94822911943193 14.6036141207344 6 26.49082928169737 39.13554921863889 18.61406329470921 15.759558204954526 7 24.949495923513446 36.81494052813523 21.6193607799083 16.616202768443028 8 25.722461140895593 33.64060631773826 20.849163034674277 19.787769506691866 9 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33.67347676731151 19.41710359790273 34-35 18.67259324967338 27.914519324870916 33.94712356636311 19.465763859092597 36-37 19.069932809063246 27.99795112838591 33.4996534996535 19.432462562897346 38-39 18.98779024086919 28.11148816381226 33.83050463469539 19.070216960623164 40-41 18.40637920708201 28.378529827517017 34.173635036953755 19.04145592844722 42-43 18.423069507374915 28.736262412031234 33.11517882965391 19.72548925093994 44-45 18.491970991190566 29.15609530914968 32.46014422445307 19.891789475206675 46-47 18.66019424495979 29.224357869644496 31.751904427334093 20.363543458061624 48-49 18.989682048982846 29.57941182496725 31.168021944778896 20.26288418127101 50-51 19.535899002828913 29.689735514019816 30.796109670739487 19.978255812411785 52-53 18.99279049275614 30.1624037637461 30.907944681707665 19.936861061790093 54-55 18.786199281830356 29.90746243109706 31.042575478327745 20.263762808744843 56-57 19.23577010441583 30.594983460966073 29.896186683215742 20.273059751402354 58-59 19.070006735240007 29.57661968063637 30.763058112229096 20.59031547189453 60-61 19.303471436472677 29.617646739857356 30.8745648503953 20.204316973274675 62-63 19.704174097459873 29.730081457560132 30.932182966211986 19.633561478768012 64-65 19.544143890127977 29.493644499229436 31.30564602710411 19.656565583538477 66-67 19.03466709728889 29.932863906908473 30.85066768537744 20.181801310425197 68-69 18.8780053428317 30.27568481389859 30.830813944381045 20.01549589888866 70-71 18.941664286772973 29.98352752060868 30.642185694368628 20.432622498249724 72-73 18.82326143077179 29.846071150513296 30.15677313452263 21.173894284192283 74-75 18.784163541536117 30.082474524964088 30.46954082272074 20.66382111077905 76-77 18.97439605666733 29.367343372441262 31.125573211369623 20.532687359521788 78-79 18.586947090355174 29.260947321738463 31.378345416682734 20.773760171223632 80-81 18.43760806933789 30.48944038307907 30.60800788522087 20.464943662362167 82-83 18.666708445513557 30.037022485857456 30.087036586218286 21.2092324824107 84-85 18.33740006386273 29.433007392412385 30.203818509347457 22.02577403437743 86-87 18.897831354918164 29.58055027697009 30.35476512671216 21.16685324139959 88-89 19.30553948789141 28.60160708776801 30.563348372666976 21.52950505167361 90-91 18.6361083863753 28.559877374838223 31.10782000515758 21.69619423362889 92-93 18.529279908988364 29.11721604658562 30.924915399670276 21.428588644755745 94-95 18.094526523824445 28.637294835024463 31.12241208936878 22.145766551782316 96-97 18.344409565526508 28.52257322062288 30.44784932307451 22.6851678907761 98-99 18.31536527814935 28.742742279520755 30.45446767075678 22.487424771573114 100-101 18.492367492954283 28.567831052236496 31.022354708851395 21.917446745957825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1286.0 1 871.0 2 554.0 3 693.0 4 618.5 5 649.5 6 1188.0 7 2376.0 8 3680.0 9 4035.5 10 3143.0 11 2246.5 12 2092.5 13 2091.0 14 2030.0 15 1905.5 16 1816.5 17 1815.0 18 1865.0 19 1909.0 20 1975.0 21 2068.0 22 2169.0 23 2424.0 24 2666.0 25 2946.0 26 3374.0 27 3782.0 28 4131.5 29 4701.0 30 5766.5 31 6737.5 32 8024.5 33 9613.5 34 10860.5 35 11834.5 36 13628.0 37 15117.0 38 14891.0 39 14315.5 40 13748.5 41 13279.0 42 12605.0 43 12740.5 44 13466.5 45 13593.0 46 13100.0 47 13260.0 48 18129.5 49 17799.5 50 12344.5 51 10315.0 52 8777.0 53 8307.5 54 8054.0 55 7357.0 56 6569.0 57 5920.5 58 5326.0 59 4765.0 60 4255.5 61 3744.5 62 3032.0 63 2180.0 64 1519.0 65 1151.5 66 860.5 67 639.5 68 466.0 69 296.5 70 196.5 71 154.5 72 126.5 73 115.0 74 92.0 75 69.5 76 51.0 77 28.5 78 20.5 79 12.5 80 8.5 81 5.5 82 2.5 83 2.0 84 2.5 85 3.0 86 2.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3256788383319663 2 0.28358593086660877 3 0.29008026516126395 4 0.2893586724618578 5 0.27685106567215156 6 0.21503462442302648 7 0.223453205916098 8 0.22008577331886942 9 0.22080736601827552 10-11 0.22429506406540517 12-13 0.22754223121273276 14-15 0.20962267917748056 16-17 0.22176948961748372 18-19 0.20745790107926215 20-21 0.2139522353739173 22-23 0.2128698463248081 24-25 0.20276754853312232 26-27 0.21756019887094794 28-29 0.21407250082381832 30-31 0.2211681623679786 32-33 0.22176948961748372 34-35 0.21479409352322446 36-37 0.21274958087490708 38-39 0.20036223953510188 40-41 0.201204097684409 42-43 0.1989190541362896 44-45 0.2182817915703541 46-47 0.206495777480054 48-49 0.21347117357431322 50-51 0.22213028596718676 52-53 0.2279030275624358 54-55 0.22670037306342558 56-57 0.1971150723877743 58-59 0.18412640379846398 60-61 0.1932665779909416 62-63 0.19410843614024875 64-65 0.19038020719331708 66-67 0.20288781398302333 68-69 0.19206392349193138 70-71 0.19543135608916 72-73 0.21166719182579788 74-75 0.19988117773549777 76-77 0.20914161737787645 78-79 0.20469179573153864 80-81 0.19086126899292116 82-83 0.20781869742896522 84-85 0.19110179989272322 86-87 0.1949502942895559 88-89 0.20084330133470596 90-91 0.1981974614368835 92-93 0.2056539193307468 94-95 0.20132436313431007 96-97 0.1971150723877743 98-99 0.20300807943292434 100-101 0.18725330549589053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 415747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.64064696277222 #Duplication Level Percentage of deduplicated Percentage of total 1 77.85186269564784 38.64616831468871 2 11.751503555592057 11.667044785698156 3 4.045211803002162 6.024207930074088 4 1.8761223093980994 3.7252770087924785 5 1.040758043255389 2.583195129945319 6 0.6467297355663758 1.9262449490146498 7 0.4350393410002674 1.5116944039057947 8 0.30678257684217886 1.2183108473081714 9 0.24101277747956892 1.0767627180342414 >10 1.6077197491860855 15.281645665648488 >50 0.12877195422743343 4.411181777473462 >100 0.06028649542480605 5.07890421857779 >500 0.004340627670586036 1.4778955284849955 >1k 0.0028937517803906904 2.605290643281018 >5k 9.645839267968968E-4 2.7661760790726775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6526 1.569704652108133 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5028 1.2093893642046727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3637 0.8748108825800306 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1905 0.45821136412289204 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1665 0.4004839481704017 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1451 0.34901033561276446 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1197 0.28791548706304554 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1027 0.24702523409669822 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 988 0.23764452900441854 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 831 0.19988117773549777 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 719 0.17294171695766897 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 696 0.16740950626222198 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 634 0.15249659047449532 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 616 0.14816703427805852 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 600 0.14431853988122584 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.13998898368478907 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 507 0.12194916619963583 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 465 0.11184686840795004 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 421 0.10126350881666014 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4053089980204307E-4 0.0 18-19 0.0 0.0 0.0 2.4053089980204307E-4 0.0 20-21 0.0 0.0 0.0 2.4053089980204307E-4 0.0 22-23 0.0 0.0 0.0 9.621235992081723E-4 0.0 24-25 0.0 0.0 0.0 0.002285043548119409 0.0 26-27 0.0 0.0 0.0 0.004329556196436775 0.0 28-29 0.0 0.0 0.0 0.008057785143368443 0.0 30-31 0.0 0.0 0.0 0.014311588538221563 0.0 32-33 0.0 0.0 0.0 0.022489639131491025 0.0 34-35 0.0 0.0 0.0 0.030667689724760493 0.0 36-37 0.0 0.0 0.0 0.044017154663773886 0.0 38-39 0.0 0.0 0.0 0.061455644899422006 0.0 40-41 0.0 0.0 0.0 0.07889413513507013 0.0 42-43 0.0 0.0 0.0 0.09861766891883766 0.0 44-45 0.0 0.0 0.0 0.10932129396002857 0.0 46-47 0.0 0.0 0.0 0.12519633334696342 0.0 48-49 0.0 0.0 0.0 0.1402295145845911 0.0 50-51 0.0 0.0 0.0 0.1512939359754851 0.0 52-53 0.0 0.0 0.0 0.17907525490262105 0.0 54-55 0.0 0.0 0.0 0.23319470735808076 0.0 56-57 0.0 0.0 0.0 0.32447618383295607 0.0 58-59 0.0 0.0 0.0 0.4382472994393225 0.0 60-61 0.0 0.0 0.0 0.6042136203027322 0.0 62-63 0.0 0.0 0.0 0.6982612021253309 0.0 64-65 0.0 0.0 0.0 0.8000057727415952 0.0 66-67 0.0 0.0 0.0 0.9947155361313491 0.0 68-69 0.0 0.0 0.0 1.240057053929433 0.0 70-71 0.0 0.0 0.0 1.5406004132320859 0.0 72-73 0.0 0.0 0.0 1.8992319848369323 0.0 74-75 0.0 0.0 0.0 2.2439127642532597 0.0 76-77 0.0 0.0 0.0 2.540848160058882 0.0 78-79 0.0 0.0 0.0 2.794969055699741 0.0 80-81 0.0 0.0 0.0 2.9979771351326647 0.0 82-83 0.0 0.0 0.0 3.317041373720075 0.0 84-85 0.0 0.0 0.0 3.90982977628221 0.0 86-87 0.0 0.0 0.0 4.639359995381806 0.0 88-89 0.0 1.2026544990102154E-4 0.0 5.416876128991911 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 20 0.0020628264 71.33964 1 GTGGTAC 115 2.4647306E-9 37.220676 1 CTCCCGC 110 6.99838E-8 34.54309 7 GTCTAGC 70 2.0359848E-4 33.971256 1 TCCCGCT 125 2.1495907E-7 30.397919 8 TACCTGG 235 0.0 30.346313 2 CTAGAAC 65 0.0058143246 29.256958 3 ATGGGAT 1060 0.0 28.24621 5 CCCGCTG 135 4.216672E-7 28.146223 9 GTACATG 4000 0.0 28.060257 1 GGTACTC 140 5.745933E-7 27.167175 3 CATGGGT 350 0.0 27.167173 4 TACATGG 4120 0.0 27.11772 2 GTACCTG 305 0.0 26.508717 1 AGTACTC 90 8.9100556E-4 26.402983 5 GGTATCA 4835 0.0 26.361973 1 ACATGGG 3940 0.0 25.581276 3 TATGGTG 95 0.0012188534 25.022398 2 GAGTACT 3880 0.0 24.850105 12-13 GTATCAA 6535 0.0 24.744173 1 >>END_MODULE